目前,临床诊断癌症的金标准是病理学的检测。但是该操作需要进行活检或内镜活检,这是一种侵入性检查,且一定程度上受制于医师的经验水平,因此不能作为肺癌的早期诊断和常规体格检查。近年来,太赫兹光谱技术已应用于多种癌种的肿瘤组织...目前,临床诊断癌症的金标准是病理学的检测。但是该操作需要进行活检或内镜活检,这是一种侵入性检查,且一定程度上受制于医师的经验水平,因此不能作为肺癌的早期诊断和常规体格检查。近年来,太赫兹光谱技术已应用于多种癌种的肿瘤组织与正常组织之间的检测。因此,探索一种高分辨率的检测方法来区分癌组织和癌旁组织具有重要意义,可以实现癌症的早发现、早诊断、早治疗。在这项研究中,我们利用太赫兹时域光谱技术研究胃肠道肿瘤组织与正常组织之间的关系,以及胃肠道不同部位肿瘤组织之间的关系。通过测量折射率、吸收系数,我们发现肿瘤组织与正常组织的太赫兹光谱曲线存在显著差异。消化道不同部位肿瘤的太赫兹光谱曲线也表现出不同的趋势。因此,太赫兹时域光谱技术具有区分肿瘤和正常组织的潜力。At present, the golden standard for clinical diagnosis of cancer is pathological examination. However, this procedure requires a biopsy or endoscopic biopsy, which is invasive examinations and limited by physician’s level of experience. Therefore, it can not be used as an early diagnosis and routine physical examination for lung cancer. In recent years, terahertz spectroscopy has been applied to detect tumor tissues and normal tissues of many kinds of cancers. Therefore, it is of great significance to explore a high-resolution detection method to distinguish cancer tissues from para-cancer tissue, which can realize early detection, early diagnosis and early treatment of cancer. In this study, we use terahertz time-domain spectroscopy to investigate the relationship between gastrointestinal tumor tissue and normal tissue, as well as the relationship between tumor tissue at different sites of the gastrointestinal tract. By measuring the refractive index and absorption constant, we find that the terahertz spectra of tumor tissues are significantly different from those of normal tissues. The terahertz spectroscopy of tumors in different parts of digestive tract also shows different trends. Therefore, terahertz time-domain spectroscopy has the potential to differentiate tumor from normal tissue.展开更多
背景与目的:非小细胞肺癌(non-small cell lung cancer, NSCLC)是目前全球发病率最高和死亡人数最多的恶性肿瘤,而其具体的发病机制仍然未知。相关文献和既往实验研究发现m6A的阅读蛋白YTHDF2可能通过分子之间的相互作用抑制NSCLC的发...背景与目的:非小细胞肺癌(non-small cell lung cancer, NSCLC)是目前全球发病率最高和死亡人数最多的恶性肿瘤,而其具体的发病机制仍然未知。相关文献和既往实验研究发现m6A的阅读蛋白YTHDF2可能通过分子之间的相互作用抑制NSCLC的发生、发展。本研究拟通过对NSCLC患者肿瘤组织进行病理观察以及生信分析,探索NSCLC的发生发展机制,对未来产生更加有效的NSCLC治疗手段和改善NSCLC患者的预后提供参考。方法:回顾性收集2021年5月至2022年5月于青岛大学胸外科确诊肺癌并住院手术的64例NSCLC患者的肿瘤及癌旁组织(根据世界卫生组织形态学标准)。对上诉样本分别进行病理染色及生信分析。结果:64例标本的HE染色可见肿瘤组织中病理损伤明显,能明确采集样本的诊断并与癌旁组织区分。TUNEL染色可见:肿瘤组织细胞凋亡水平升高。qRT-PCR实验结果表明在肿瘤组织中YTHDF2和p14基因表达水平明显高于癌旁组织(P ARF蛋白的表达,达到延缓NSCLC发生、发展和改善NSCLC患者预后的作用。Background and objective: Non-small cell lung cancer (NSCLC) is currently the most prevalent and deadly malignancy worldwide. However, the precise mechanisms underlying its pathogenesis remain largely unknown. Previous literature and experimental studies suggest that YTHDF2, an m6A reader protein, may inhibit the initiation and progression of NSCLC through molecular interactions. This study aims to explore the mechanisms involved in the development and progression of NSCLC by conducting pathological observations and bioinformatic analyses of tumor tissues from NSCLC patients. The findings are expected to contribute to the development of more effective treatment strategies for NSCLC and to improve the prognosis of NSCLC patients. Methods: We retrospectively collected tumor and adjacent normal tissues from 64 NSCLC patients who were diagnosed and underwent surgery between May 2021 and May 2022 in the Department of Thoracic Surgery at Qingdao University, following the World Health Organization (WHO) morphological criteria. Pathological staining and bioinformatic analyses were performed on the collected samples. Results: Hematoxylin and eosin staining of the 64 specimens revealed significant pathological damage within the tumor tissue, which could clearly diagnose the collected samples and distinguish them from the adjacent tissues. TUNEL staining showed that the level of apoptosis in tumor tissues was increased compared with adjacent tissues. The results of qRT-PCR showed that the expression levels of YTHDF2 and p14 genes in tumor tissues of NSCLC patients were significantly higher than those in adjacent tissues (P ARF protein expression. It can delay the occurrence and development of NSCLC and improve the prognosis of NSCLC patients.展开更多
目的探究重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)治疗前后肿瘤患者外周血淋巴细胞亚群的变化及临床意义。方法 选取2018年3月—2018年10月在我院就诊的30例化疗或放疗后的恶性肿瘤患者作为研究对象。患者放化疗后出现白细胞降低时...目的探究重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)治疗前后肿瘤患者外周血淋巴细胞亚群的变化及临床意义。方法 选取2018年3月—2018年10月在我院就诊的30例化疗或放疗后的恶性肿瘤患者作为研究对象。患者放化疗后出现白细胞降低时使用rhGM-CSF治疗,采用流式细胞术检测治疗前后患者外周血中淋巴细胞亚群水平的变化。结果 患者治疗后外周血淋巴细胞亚群中CD3 + CD4 +细胞百分率较治疗前明显升高( t =2.826, P <0.05),CD3 + CD8 +细胞百分率明显下降( t =2.932, P <0.05),CD4/CD8比值明显升高( t =3.509, P <0.05)。结论 rhGM-CSF能够增强肿瘤患者机体的免疫应答,提高患者的免疫功能,抑制肿瘤的发生与发展。展开更多
文摘目前,临床诊断癌症的金标准是病理学的检测。但是该操作需要进行活检或内镜活检,这是一种侵入性检查,且一定程度上受制于医师的经验水平,因此不能作为肺癌的早期诊断和常规体格检查。近年来,太赫兹光谱技术已应用于多种癌种的肿瘤组织与正常组织之间的检测。因此,探索一种高分辨率的检测方法来区分癌组织和癌旁组织具有重要意义,可以实现癌症的早发现、早诊断、早治疗。在这项研究中,我们利用太赫兹时域光谱技术研究胃肠道肿瘤组织与正常组织之间的关系,以及胃肠道不同部位肿瘤组织之间的关系。通过测量折射率、吸收系数,我们发现肿瘤组织与正常组织的太赫兹光谱曲线存在显著差异。消化道不同部位肿瘤的太赫兹光谱曲线也表现出不同的趋势。因此,太赫兹时域光谱技术具有区分肿瘤和正常组织的潜力。At present, the golden standard for clinical diagnosis of cancer is pathological examination. However, this procedure requires a biopsy or endoscopic biopsy, which is invasive examinations and limited by physician’s level of experience. Therefore, it can not be used as an early diagnosis and routine physical examination for lung cancer. In recent years, terahertz spectroscopy has been applied to detect tumor tissues and normal tissues of many kinds of cancers. Therefore, it is of great significance to explore a high-resolution detection method to distinguish cancer tissues from para-cancer tissue, which can realize early detection, early diagnosis and early treatment of cancer. In this study, we use terahertz time-domain spectroscopy to investigate the relationship between gastrointestinal tumor tissue and normal tissue, as well as the relationship between tumor tissue at different sites of the gastrointestinal tract. By measuring the refractive index and absorption constant, we find that the terahertz spectra of tumor tissues are significantly different from those of normal tissues. The terahertz spectroscopy of tumors in different parts of digestive tract also shows different trends. Therefore, terahertz time-domain spectroscopy has the potential to differentiate tumor from normal tissue.
文摘背景与目的:非小细胞肺癌(non-small cell lung cancer, NSCLC)是目前全球发病率最高和死亡人数最多的恶性肿瘤,而其具体的发病机制仍然未知。相关文献和既往实验研究发现m6A的阅读蛋白YTHDF2可能通过分子之间的相互作用抑制NSCLC的发生、发展。本研究拟通过对NSCLC患者肿瘤组织进行病理观察以及生信分析,探索NSCLC的发生发展机制,对未来产生更加有效的NSCLC治疗手段和改善NSCLC患者的预后提供参考。方法:回顾性收集2021年5月至2022年5月于青岛大学胸外科确诊肺癌并住院手术的64例NSCLC患者的肿瘤及癌旁组织(根据世界卫生组织形态学标准)。对上诉样本分别进行病理染色及生信分析。结果:64例标本的HE染色可见肿瘤组织中病理损伤明显,能明确采集样本的诊断并与癌旁组织区分。TUNEL染色可见:肿瘤组织细胞凋亡水平升高。qRT-PCR实验结果表明在肿瘤组织中YTHDF2和p14基因表达水平明显高于癌旁组织(P ARF蛋白的表达,达到延缓NSCLC发生、发展和改善NSCLC患者预后的作用。Background and objective: Non-small cell lung cancer (NSCLC) is currently the most prevalent and deadly malignancy worldwide. However, the precise mechanisms underlying its pathogenesis remain largely unknown. Previous literature and experimental studies suggest that YTHDF2, an m6A reader protein, may inhibit the initiation and progression of NSCLC through molecular interactions. This study aims to explore the mechanisms involved in the development and progression of NSCLC by conducting pathological observations and bioinformatic analyses of tumor tissues from NSCLC patients. The findings are expected to contribute to the development of more effective treatment strategies for NSCLC and to improve the prognosis of NSCLC patients. Methods: We retrospectively collected tumor and adjacent normal tissues from 64 NSCLC patients who were diagnosed and underwent surgery between May 2021 and May 2022 in the Department of Thoracic Surgery at Qingdao University, following the World Health Organization (WHO) morphological criteria. Pathological staining and bioinformatic analyses were performed on the collected samples. Results: Hematoxylin and eosin staining of the 64 specimens revealed significant pathological damage within the tumor tissue, which could clearly diagnose the collected samples and distinguish them from the adjacent tissues. TUNEL staining showed that the level of apoptosis in tumor tissues was increased compared with adjacent tissues. The results of qRT-PCR showed that the expression levels of YTHDF2 and p14 genes in tumor tissues of NSCLC patients were significantly higher than those in adjacent tissues (P ARF protein expression. It can delay the occurrence and development of NSCLC and improve the prognosis of NSCLC patients.
文摘目的探究重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)治疗前后肿瘤患者外周血淋巴细胞亚群的变化及临床意义。方法 选取2018年3月—2018年10月在我院就诊的30例化疗或放疗后的恶性肿瘤患者作为研究对象。患者放化疗后出现白细胞降低时使用rhGM-CSF治疗,采用流式细胞术检测治疗前后患者外周血中淋巴细胞亚群水平的变化。结果 患者治疗后外周血淋巴细胞亚群中CD3 + CD4 +细胞百分率较治疗前明显升高( t =2.826, P <0.05),CD3 + CD8 +细胞百分率明显下降( t =2.932, P <0.05),CD4/CD8比值明显升高( t =3.509, P <0.05)。结论 rhGM-CSF能够增强肿瘤患者机体的免疫应答,提高患者的免疫功能,抑制肿瘤的发生与发展。
