目的:建立A549与A549/DDP两组细胞株的双向凝胶电泳图谱,识别并鉴定其差异表达的蛋白质,筛选肺腺癌耐药相关蛋白质。方法:收集A549与A549/DDP两组细胞株的总蛋白质,应用二维凝胶电泳(two-dimensional gel electrophoresis,2-DE)进行分...目的:建立A549与A549/DDP两组细胞株的双向凝胶电泳图谱,识别并鉴定其差异表达的蛋白质,筛选肺腺癌耐药相关蛋白质。方法:收集A549与A549/DDP两组细胞株的总蛋白质,应用二维凝胶电泳(two-dimensional gel electrophoresis,2-DE)进行分离应用,图像分析识别差异表达的蛋白质点,采用基质辅助激光解吸电离飞行时间质谱鉴定差异蛋白质点。使用Western免疫印迹对其中4个蛋白质进行验证。结果:建立了A549与A549/DDP细胞蛋白质的2-DE图谱,识别了40个差异表达的蛋白质点,鉴定了23个差异表达蛋白质。Western免疫印迹证实了差异蛋白质热休克蛋白β1,膜联蛋白A4,丝切蛋白l和波形蛋白在A549与A549/DDP细胞中的差异表达水平。结论:23个差异表达蛋白质为研究肺腺癌耐药机制提供了实验依据。展开更多
Objective: To study the effects of platelet activation and endothelial cell injury on the patients with malignant tumor and their prognoses.Methods: Radioimmunity and ELISA methods were employed to detect the TXB2, GM...Objective: To study the effects of platelet activation and endothelial cell injury on the patients with malignant tumor and their prognoses.Methods: Radioimmunity and ELISA methods were employed to detect the TXB2, GMP-140, vWF, cGMP and FN in 78 cases of malignant tumor and 40 healthy control persons.Results: The levels of TXB2, MP-140 and cGMP were increased in intestinal cancer group, lung cancer group and hepatic cancer group, while FN decreased in intestinal cancer and lung cancer group. cGMP was positively related to TXB2, GMP-140, vWF in malignant tumor group. FN was decreased in the group complicated with infection and the group with metastasis, while the other indexes increased. GMP-140, vWF and cGMP was decreased after operation except for the increasing of FN.Conclusion: Activations of platelet and injury of endothelial cells developed in patients with malignant tumor, and both of them affected the metastasis and prognosis of malignant tumor. Key words platelet activation - epithelium injury - malignant tumor - metastasis This work was supported by grants from Guangdong Medical Science foundation (A2000633).展开更多
文摘目的:建立A549与A549/DDP两组细胞株的双向凝胶电泳图谱,识别并鉴定其差异表达的蛋白质,筛选肺腺癌耐药相关蛋白质。方法:收集A549与A549/DDP两组细胞株的总蛋白质,应用二维凝胶电泳(two-dimensional gel electrophoresis,2-DE)进行分离应用,图像分析识别差异表达的蛋白质点,采用基质辅助激光解吸电离飞行时间质谱鉴定差异蛋白质点。使用Western免疫印迹对其中4个蛋白质进行验证。结果:建立了A549与A549/DDP细胞蛋白质的2-DE图谱,识别了40个差异表达的蛋白质点,鉴定了23个差异表达蛋白质。Western免疫印迹证实了差异蛋白质热休克蛋白β1,膜联蛋白A4,丝切蛋白l和波形蛋白在A549与A549/DDP细胞中的差异表达水平。结论:23个差异表达蛋白质为研究肺腺癌耐药机制提供了实验依据。
基金This work was supported by grants from Guangdong Medical Science foundation(A2000633).
文摘Objective: To study the effects of platelet activation and endothelial cell injury on the patients with malignant tumor and their prognoses.Methods: Radioimmunity and ELISA methods were employed to detect the TXB2, GMP-140, vWF, cGMP and FN in 78 cases of malignant tumor and 40 healthy control persons.Results: The levels of TXB2, MP-140 and cGMP were increased in intestinal cancer group, lung cancer group and hepatic cancer group, while FN decreased in intestinal cancer and lung cancer group. cGMP was positively related to TXB2, GMP-140, vWF in malignant tumor group. FN was decreased in the group complicated with infection and the group with metastasis, while the other indexes increased. GMP-140, vWF and cGMP was decreased after operation except for the increasing of FN.Conclusion: Activations of platelet and injury of endothelial cells developed in patients with malignant tumor, and both of them affected the metastasis and prognosis of malignant tumor. Key words platelet activation - epithelium injury - malignant tumor - metastasis This work was supported by grants from Guangdong Medical Science foundation (A2000633).
