[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ t...[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. [Result] All strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of A factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mat- ing types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isozyme test, Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing the results of mating genotype analysis and esterase isozyme analysis, it showed great similarity. [Conclusion] Mating genotype analysis could be used as an important supplementary method for strain identification and genetic diversity research.展开更多
[Objective] This study aimed to screen out the methods and indices that could quickly and accurately detect the maturity of materials through evaluating the mature status of rapeseed meal composting. [Method] The phys...[Objective] This study aimed to screen out the methods and indices that could quickly and accurately detect the maturity of materials through evaluating the mature status of rapeseed meal composting. [Method] The physicochemical proper- ties variation of rapeseed meal composting was analyzed by testing emission of carbon dioxide, degradation of organic matter, conductivity values, coefficient of seed germination and free amino acids during the fermentation process. [Result] In the process of fermentation, the release amount of carbon dioxide per unit time was drastically reduced at the beginning and then the change became smoothly later. The organic matter was degraded quickly and then slowed down, and the total amount of degradation was 7.34%, total degradation rated of 9.06%. The seed ger- mination coefficient showed a trend of gradual decline, and after the start of fermen- tation, the germination coefficient was below the critical coefficient of 0.8. The con- ductivity value and the free amino acid content changed significantly, which showed strong regularity, and the increase amount was close to 1 times. [Conclusion] The detection of conductivity value and free amino acid content in rapeseed meal com- posting can be used as the methods and indices for the quick and accurate detec- tion of the maturity of organic fertilizer fermentation.展开更多
基金Supported by National Science and Technology Support Program"Regional Development of Chinese Traditional Medicine Industry and Research of Characteristic Product"(2006BIA06A20)Personnel Services Business Action Plan of Ministry ofScience and Technology "Development of Series Products of Rare Edible Fungus"(2009GJD20012)~~
文摘[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. [Result] All strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of A factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mat- ing types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isozyme test, Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing the results of mating genotype analysis and esterase isozyme analysis, it showed great similarity. [Conclusion] Mating genotype analysis could be used as an important supplementary method for strain identification and genetic diversity research.
基金Supported by the Research and Development of Featured Sound Flavored Tobacco of Hunan Province(2011NK4057)~~
文摘[Objective] This study aimed to screen out the methods and indices that could quickly and accurately detect the maturity of materials through evaluating the mature status of rapeseed meal composting. [Method] The physicochemical proper- ties variation of rapeseed meal composting was analyzed by testing emission of carbon dioxide, degradation of organic matter, conductivity values, coefficient of seed germination and free amino acids during the fermentation process. [Result] In the process of fermentation, the release amount of carbon dioxide per unit time was drastically reduced at the beginning and then the change became smoothly later. The organic matter was degraded quickly and then slowed down, and the total amount of degradation was 7.34%, total degradation rated of 9.06%. The seed ger- mination coefficient showed a trend of gradual decline, and after the start of fermen- tation, the germination coefficient was below the critical coefficient of 0.8. The con- ductivity value and the free amino acid content changed significantly, which showed strong regularity, and the increase amount was close to 1 times. [Conclusion] The detection of conductivity value and free amino acid content in rapeseed meal com- posting can be used as the methods and indices for the quick and accurate detec- tion of the maturity of organic fertilizer fermentation.
