目的:通过实证干预的方法,探索正念认知疗法对医学生焦虑情绪的干预效果;方法:通过SAS焦虑自评量表筛选出40名焦虑分值大于50的医学生,按照随机化原则分成两组,分为实验组和对照组,一组20人。对照组学生不进行任何处理,实验组学生由专...目的:通过实证干预的方法,探索正念认知疗法对医学生焦虑情绪的干预效果;方法:通过SAS焦虑自评量表筛选出40名焦虑分值大于50的医学生,按照随机化原则分成两组,分为实验组和对照组,一组20人。对照组学生不进行任何处理,实验组学生由专业老师指导,进行为期8周的正念认知训练,一周练习1次,每次练习45分钟,有具体的主题和内容。8周练习结束后,再次通过SAS量表对两组被试的焦虑水平进行测量,检验正念认知疗法对医学生焦虑情绪的干预效果如何。结果:实验组学生在进行8周正念认知训练后,实验组的焦虑得分显著下降。结论:正念认知疗法对医学生焦虑情绪的干预效果显著。Purpose: To explore the effect of Mindfulness-Based Cognitive Therapy (MBCT) on the anxiety of medical students through empirical intervention methods. Method: Forty medical students with an anxiety score greater than 50, as measured by the Self-Rating Anxiety Scale (SAS), were randomly divided into two groups: an experimental group and a control group, with 20 students in each group. The control group did not receive any intervention, while the experimental group received an 8-week mindfulness-based cognitive training guided by a professional instructor, with one session per week, each lasting 45 minutes, and having specific themes and content. After the 8-week training, the anxiety levels of both groups were measured again using the SAS to test the effect of MBCT on the anxiety of medical students. Results: The anxiety scores of the experimental group significantly decreased after the 8-week MBCT training. Conclusion: Mindfulness-Based Cognitive Therapy has a significant intervention effect on the anxiety of medical students.展开更多
目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UV...目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UVA和H2O2分别处理细胞使其衰老,建立早衰模型。此后进行CPE的慢病毒转染,获得CPE稳定敲除的细胞株。通过qRT-PCR和WB技术,检测CPE和衰老标志物蛋白及mRNA的表达。分别采用cck8和SA-β-gal染色检测CPE敲降后NHSFs的增殖和衰老程度。最后采用qRT-PCR检测CPE敲降后NHSFs促炎症因子的表达水平。结果:N1组CPE蛋白和mRNA表达最高,其次是N2组,N3组最少,早衰细胞模型中CPE蛋白和mRNA的表达减低,CPE敲降后NHSFs蛋白和mRNA的表达均升高、细胞活性降低、细胞衰老程度明显加快和促炎症因子mRNA表达升高。结论:CPE可作为潜在衰老标志物且其高表达可延缓衰老。Objective: To explore the correlation between the expression of CPE and cellular senescence. Methods: Human foreskin fibroblasts (NHSF cells) isolated from healthy human donors (5~20 years old) who underwent surgical defibrillation were selected for passage. Here, we defined “NHSFs of less than 15 generations as group N1, NHSFs of 15~25 generations as group N2, and NHSF of more than 45 generations as group N3”. Then the cells were treated with UVA and H2O2 respectively to make them senescent, and the premature aging model was established. Then lentiviral transfection of CPE was performed to obtain stable CPE knockout cell lines. The expression of CPE and aging marker protein and mRNA were detected by qRT-PCR and WB. The proliferation and senescence of NHSFs after CPE knockdown were detected by cck8 and SA-β-gal staining, respectively. Finally, the expression level of NHSFs proinflammatory factors after CPE knock-down was detected by qRT-PCR. Results: The expressions of CPE protein and mRNA were the highest in N1 group, followed by N2 group and N3 group, and the expressions of CPE protein and mRNA were decreased in the progeria cell model. After CPE knockdown, the expressions of NHSFs protein and mRNA were both increased, the cell activity was decreased, the degree of cell senility was significantly accelerated, and the mRNA expression of proinflammatory factors was increased. Conclusion: CPE can be used as a potential senescence marker and its high expression can delay senescence.展开更多
转化生长因子-β (Transforming growth factor-beta, TGF-β)作为一种细胞因子,隶属于TGF-β超家族,它在细胞生长和分化过程中担任着关键的调节角色。近年来许多研究表明TGF-β在结直肠癌发生和发展过程中的作用具有两面性,它既有可以...转化生长因子-β (Transforming growth factor-beta, TGF-β)作为一种细胞因子,隶属于TGF-β超家族,它在细胞生长和分化过程中担任着关键的调节角色。近年来许多研究表明TGF-β在结直肠癌发生和发展过程中的作用具有两面性,它既有可以促进肿瘤的生长和转移,也有可以在某些特定情况下通过抑制TGF-β的活性来增强结直肠癌的治疗效果。本文旨在综述近年来TGF-β在结直肠癌发生和发展过程中的双重作用及潜在的机制,以期为结直肠癌的治疗提供参考依据。Transforming growth factor-beta (TGF-β), a cytokine belonging to the TGF-β superfamily, plays a key regulatory role in the process of cell growth and differentiation. In recent years, many studies have shown that the role of TGF-β in the occurrence and development of colorectal cancer has two sides, which can promote the growth and metastasis of tumors, and can enhance the therapeutic effect of colorectal cancer by inhibiting the activity of TGF-β in some specific cases. This article aims to review the dual role of TGF-β in the occurrence and development of colorectal cancer in recent years and its potential mechanism, in order to provide a reference for the treatment of colorectal cancer.展开更多
食品化学是食品卫生与营养学专业最早接触的一门专业核心课程,但该课程理论性较强,学生在传统教学方法中参与度低,难以消化复杂知识。为提高学生课堂参与度,促进学生的主动学习和深度学习,本文以“淀粉糊化与老化”为例,将BOPPPS模型融...食品化学是食品卫生与营养学专业最早接触的一门专业核心课程,但该课程理论性较强,学生在传统教学方法中参与度低,难以消化复杂知识。为提高学生课堂参与度,促进学生的主动学习和深度学习,本文以“淀粉糊化与老化”为例,将BOPPPS模型融入教学环节,构建知识点与生活现象的关联,使案例讨论可测评,全过程运用在线资源,以期提高课程质量。Food chemistry is a core professional course for food hygiene and nutrition majors. However, this course emphasizes theory significantly, and students participate little in traditional teaching methods, which makes it difficult to digest complex knowledge. In order to improve students’ classroom participation and promote students’ active learning and deep learning, this paper takes “Starch Gelatinization and Aging” as an example, integrates the BOPPPS model into the teaching process, builds the correlation between knowledge points and life phenomena, makes case discussion evaluable, and utilizes online resources in the whole process to improve the course quality.展开更多
文章旨在探讨如何在生物化学与分子生物学的教学过程中融入科研元素,以培养学生创新思维和科研实践能力。通过对课程内容整合,结合前沿科研成果,改革教学手段,开展了一种全新的教学模式。文中介绍了该模式的设计思路、具体实施过程以及...文章旨在探讨如何在生物化学与分子生物学的教学过程中融入科研元素,以培养学生创新思维和科研实践能力。通过对课程内容整合,结合前沿科研成果,改革教学手段,开展了一种全新的教学模式。文中介绍了该模式的设计思路、具体实施过程以及教学效果评估,结果显示,科研导向的教学方法显著提高了学生对生物化学与分子生物学理论知识的理解深度,增强了其动手能力和科研素养。This paper aims to explore how to integrate scientific research elements into the teaching process of biochemistry and molecular biology, so as to cultivate students’ innovative thinking and scientific research practice ability. Through the integration of the curriculum content, combining the advanced scientific research results and the reform of teaching methods, a new teaching model is developed. This paper introduces the design idea, implementation process, and teaching effect evaluation of the model. The results show that the research-oriented teaching method significantly improves students’ understanding of the theoretical knowledge of biochemistry and molecular biology, and enhances their hands-on ability and scientific research accomplishment.展开更多
基础营养学实践教学存在内容陈旧、教学理念落后、教学方法单一、实践教学环节薄弱、考核评价体系不完善等问题。因此,本研究在基础营养学实践教学中更新教学内容,融入“双创”理念,增加综合性实验、设计性实验和探索性实验的比例,创新...基础营养学实践教学存在内容陈旧、教学理念落后、教学方法单一、实践教学环节薄弱、考核评价体系不完善等问题。因此,本研究在基础营养学实践教学中更新教学内容,融入“双创”理念,增加综合性实验、设计性实验和探索性实验的比例,创新教学模式,加强实践教学环节,构建多元化的教学评价体系,从而为提高基础营养学实践教学效果提供强有力的支撑。There are some problems in the practical teaching of Fundamental Nutrition, such as old content, backward teaching idea, single teaching method, weak practice teaching link and imperfect evaluation system. Therefore, this study updates the teaching content in the practical teaching of Fundamental Nutrition, integrates the concept of “double creation”, increases the proportion of comprehensive experiment, designed experiment and exploratory experiment, innovates the teaching mode, strengthens the practical teaching links, and builds a diversified teaching evaluation system, thus providing strong support for improving the practical teaching effect of basic nutrition.展开更多
文摘目的:通过实证干预的方法,探索正念认知疗法对医学生焦虑情绪的干预效果;方法:通过SAS焦虑自评量表筛选出40名焦虑分值大于50的医学生,按照随机化原则分成两组,分为实验组和对照组,一组20人。对照组学生不进行任何处理,实验组学生由专业老师指导,进行为期8周的正念认知训练,一周练习1次,每次练习45分钟,有具体的主题和内容。8周练习结束后,再次通过SAS量表对两组被试的焦虑水平进行测量,检验正念认知疗法对医学生焦虑情绪的干预效果如何。结果:实验组学生在进行8周正念认知训练后,实验组的焦虑得分显著下降。结论:正念认知疗法对医学生焦虑情绪的干预效果显著。Purpose: To explore the effect of Mindfulness-Based Cognitive Therapy (MBCT) on the anxiety of medical students through empirical intervention methods. Method: Forty medical students with an anxiety score greater than 50, as measured by the Self-Rating Anxiety Scale (SAS), were randomly divided into two groups: an experimental group and a control group, with 20 students in each group. The control group did not receive any intervention, while the experimental group received an 8-week mindfulness-based cognitive training guided by a professional instructor, with one session per week, each lasting 45 minutes, and having specific themes and content. After the 8-week training, the anxiety levels of both groups were measured again using the SAS to test the effect of MBCT on the anxiety of medical students. Results: The anxiety scores of the experimental group significantly decreased after the 8-week MBCT training. Conclusion: Mindfulness-Based Cognitive Therapy has a significant intervention effect on the anxiety of medical students.
文摘目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UVA和H2O2分别处理细胞使其衰老,建立早衰模型。此后进行CPE的慢病毒转染,获得CPE稳定敲除的细胞株。通过qRT-PCR和WB技术,检测CPE和衰老标志物蛋白及mRNA的表达。分别采用cck8和SA-β-gal染色检测CPE敲降后NHSFs的增殖和衰老程度。最后采用qRT-PCR检测CPE敲降后NHSFs促炎症因子的表达水平。结果:N1组CPE蛋白和mRNA表达最高,其次是N2组,N3组最少,早衰细胞模型中CPE蛋白和mRNA的表达减低,CPE敲降后NHSFs蛋白和mRNA的表达均升高、细胞活性降低、细胞衰老程度明显加快和促炎症因子mRNA表达升高。结论:CPE可作为潜在衰老标志物且其高表达可延缓衰老。Objective: To explore the correlation between the expression of CPE and cellular senescence. Methods: Human foreskin fibroblasts (NHSF cells) isolated from healthy human donors (5~20 years old) who underwent surgical defibrillation were selected for passage. Here, we defined “NHSFs of less than 15 generations as group N1, NHSFs of 15~25 generations as group N2, and NHSF of more than 45 generations as group N3”. Then the cells were treated with UVA and H2O2 respectively to make them senescent, and the premature aging model was established. Then lentiviral transfection of CPE was performed to obtain stable CPE knockout cell lines. The expression of CPE and aging marker protein and mRNA were detected by qRT-PCR and WB. The proliferation and senescence of NHSFs after CPE knockdown were detected by cck8 and SA-β-gal staining, respectively. Finally, the expression level of NHSFs proinflammatory factors after CPE knock-down was detected by qRT-PCR. Results: The expressions of CPE protein and mRNA were the highest in N1 group, followed by N2 group and N3 group, and the expressions of CPE protein and mRNA were decreased in the progeria cell model. After CPE knockdown, the expressions of NHSFs protein and mRNA were both increased, the cell activity was decreased, the degree of cell senility was significantly accelerated, and the mRNA expression of proinflammatory factors was increased. Conclusion: CPE can be used as a potential senescence marker and its high expression can delay senescence.
文摘转化生长因子-β (Transforming growth factor-beta, TGF-β)作为一种细胞因子,隶属于TGF-β超家族,它在细胞生长和分化过程中担任着关键的调节角色。近年来许多研究表明TGF-β在结直肠癌发生和发展过程中的作用具有两面性,它既有可以促进肿瘤的生长和转移,也有可以在某些特定情况下通过抑制TGF-β的活性来增强结直肠癌的治疗效果。本文旨在综述近年来TGF-β在结直肠癌发生和发展过程中的双重作用及潜在的机制,以期为结直肠癌的治疗提供参考依据。Transforming growth factor-beta (TGF-β), a cytokine belonging to the TGF-β superfamily, plays a key regulatory role in the process of cell growth and differentiation. In recent years, many studies have shown that the role of TGF-β in the occurrence and development of colorectal cancer has two sides, which can promote the growth and metastasis of tumors, and can enhance the therapeutic effect of colorectal cancer by inhibiting the activity of TGF-β in some specific cases. This article aims to review the dual role of TGF-β in the occurrence and development of colorectal cancer in recent years and its potential mechanism, in order to provide a reference for the treatment of colorectal cancer.
文摘食品化学是食品卫生与营养学专业最早接触的一门专业核心课程,但该课程理论性较强,学生在传统教学方法中参与度低,难以消化复杂知识。为提高学生课堂参与度,促进学生的主动学习和深度学习,本文以“淀粉糊化与老化”为例,将BOPPPS模型融入教学环节,构建知识点与生活现象的关联,使案例讨论可测评,全过程运用在线资源,以期提高课程质量。Food chemistry is a core professional course for food hygiene and nutrition majors. However, this course emphasizes theory significantly, and students participate little in traditional teaching methods, which makes it difficult to digest complex knowledge. In order to improve students’ classroom participation and promote students’ active learning and deep learning, this paper takes “Starch Gelatinization and Aging” as an example, integrates the BOPPPS model into the teaching process, builds the correlation between knowledge points and life phenomena, makes case discussion evaluable, and utilizes online resources in the whole process to improve the course quality.
文摘文章旨在探讨如何在生物化学与分子生物学的教学过程中融入科研元素,以培养学生创新思维和科研实践能力。通过对课程内容整合,结合前沿科研成果,改革教学手段,开展了一种全新的教学模式。文中介绍了该模式的设计思路、具体实施过程以及教学效果评估,结果显示,科研导向的教学方法显著提高了学生对生物化学与分子生物学理论知识的理解深度,增强了其动手能力和科研素养。This paper aims to explore how to integrate scientific research elements into the teaching process of biochemistry and molecular biology, so as to cultivate students’ innovative thinking and scientific research practice ability. Through the integration of the curriculum content, combining the advanced scientific research results and the reform of teaching methods, a new teaching model is developed. This paper introduces the design idea, implementation process, and teaching effect evaluation of the model. The results show that the research-oriented teaching method significantly improves students’ understanding of the theoretical knowledge of biochemistry and molecular biology, and enhances their hands-on ability and scientific research accomplishment.
文摘基础营养学实践教学存在内容陈旧、教学理念落后、教学方法单一、实践教学环节薄弱、考核评价体系不完善等问题。因此,本研究在基础营养学实践教学中更新教学内容,融入“双创”理念,增加综合性实验、设计性实验和探索性实验的比例,创新教学模式,加强实践教学环节,构建多元化的教学评价体系,从而为提高基础营养学实践教学效果提供强有力的支撑。There are some problems in the practical teaching of Fundamental Nutrition, such as old content, backward teaching idea, single teaching method, weak practice teaching link and imperfect evaluation system. Therefore, this study updates the teaching content in the practical teaching of Fundamental Nutrition, integrates the concept of “double creation”, increases the proportion of comprehensive experiment, designed experiment and exploratory experiment, innovates the teaching mode, strengthens the practical teaching links, and builds a diversified teaching evaluation system, thus providing strong support for improving the practical teaching effect of basic nutrition.
