喹诺酮类药物作为一种具备高效良好性的抗菌灭毒药物,在具备抗病毒和抗真菌的作用的同时,还具有很好的利用度和耐受性,在水产养殖病害防治领域中广泛使用。随着应用越来越普遍,喹诺酮的耐药性、药物残留问题屡见不鲜,给水产养殖业的发...喹诺酮类药物作为一种具备高效良好性的抗菌灭毒药物,在具备抗病毒和抗真菌的作用的同时,还具有很好的利用度和耐受性,在水产养殖病害防治领域中广泛使用。随着应用越来越普遍,喹诺酮的耐药性、药物残留问题屡见不鲜,给水产养殖业的发展及人类的生活都带来了极大的危害。本文将现有关于喹诺酮类药物残留的检测技术及研究进展做一简单综述和分析,希望能为今后的水产品安全检测工作提供一些帮助和思考。Quinolones, as highly efficient and effective antibacterial and antiviral drugs, not only have antiviral and antifungal effects, but also have affordable properties with good utilization and tolerance. They are widely used in the field of aquaculture disease prevention and control. With the increasing popularity of quinolone applications, the problems of quinolone resistance and drug residue are not uncommon, which have brought great harm to the development of aquaculture and human life. This article provides a brief review and analysis of the existing detection techniques and research progress on quinolone drug residues, hoping to provide some help and reflection for future aquatic product safety testing work.展开更多
文摘喹诺酮类药物作为一种具备高效良好性的抗菌灭毒药物,在具备抗病毒和抗真菌的作用的同时,还具有很好的利用度和耐受性,在水产养殖病害防治领域中广泛使用。随着应用越来越普遍,喹诺酮的耐药性、药物残留问题屡见不鲜,给水产养殖业的发展及人类的生活都带来了极大的危害。本文将现有关于喹诺酮类药物残留的检测技术及研究进展做一简单综述和分析,希望能为今后的水产品安全检测工作提供一些帮助和思考。Quinolones, as highly efficient and effective antibacterial and antiviral drugs, not only have antiviral and antifungal effects, but also have affordable properties with good utilization and tolerance. They are widely used in the field of aquaculture disease prevention and control. With the increasing popularity of quinolone applications, the problems of quinolone resistance and drug residue are not uncommon, which have brought great harm to the development of aquaculture and human life. This article provides a brief review and analysis of the existing detection techniques and research progress on quinolone drug residues, hoping to provide some help and reflection for future aquatic product safety testing work.
文摘目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定罗非鱼中氯霉素残留量。方法样品采用乙酸乙酯为溶剂提取,无水硫酸钠脱水,提取液氮气吹干,以10%甲醇溶液溶解,冷冻除去脂肪,经C18固相萃取小柱净化,采用C18固相萃取柱分离,流动相甲醇-水,梯度洗脱,流速为1 m L/min,紫外检测波长280 nm。结果在0.1~5μg/m L范围内,氯霉素浓度和峰面积线性关系良好,精密度较好,相对标准偏差(relative standard deviation,RSD)在3.12%~7.53%之间,检测限为0.02μg/g,回收率80.5%~107.1%。结论该方法前处理方法简单、快捷,对实验员身体危害小,准确度较好,适用于罗非鱼中氯霉素含量的测定。
