IC 50 values of 13 flavonoids to lipid peroxidation induced by OH · in liver microsomer from rat were evaluated and the relationship between structure and activity was studied. The flavonoids with catechol group,...IC 50 values of 13 flavonoids to lipid peroxidation induced by OH · in liver microsomer from rat were evaluated and the relationship between structure and activity was studied. The flavonoids with catechol group, which usually have a higher energy in the highest occupied molecular orbital and lower ΔHOF values, have higher inhibitory activities than those without catechol group. The flavonoids without catechol group in B ring not being suitable structure to chelate with Fe 2+ have little inhibitory activities. The results suggest the anti-lipid peroxidation activity of flavonoids is related with the structure of flavonoids.展开更多
目的:分离紫心甘薯的多糖(Polysaccharide of purple sweet potato,PPSP)组分,并对其组分的抑癌活性进行初步研究。方法:采用DEAE-Cellulose和CM-Cellulose离子交换柱分离紫心甘薯多糖,收集分离PPSP组分;MTT法检测PPSP各组分对肿瘤细胞...目的:分离紫心甘薯的多糖(Polysaccharide of purple sweet potato,PPSP)组分,并对其组分的抑癌活性进行初步研究。方法:采用DEAE-Cellulose和CM-Cellulose离子交换柱分离紫心甘薯多糖,收集分离PPSP组分;MTT法检测PPSP各组分对肿瘤细胞的体外抑制活性;薄层层析法分析PPSP各组分的单糖组成;利用傅里叶红外光谱技术检测PPSP组分的结构特征。结果:选用弱阴离子交换柱DEAE-Cellulose分离,并经NaCl溶液梯度分段洗脱,得到4个紫心甘薯多糖组分,分别命名为PPSPⅠ、PPSPⅡ、PPSPⅢ和PPSPⅣ;观察PPSPⅠ、PPSPⅡ、PPSPⅢ对Hela和HepG2肿瘤细胞的抑制作用,检测发现PPSPⅡ、PPSPⅢ组分有一定的抑癌活性。结构分析结果表明,PPSPⅠ主要由葡萄糖和半乳糖这两种单糖组成;PPSPⅡ由葡萄糖组成,并具有β-D-葡萄吡喃聚糖的红外特征吸收峰;PPSPⅢ具有蛋白吸收峰。结论:成功分离得到PPSP的4种多糖组分,其中检测到PPSPⅡ、PPSPⅢ组分对Hela和HepG2肿瘤细胞具有一定的体外抑制作用。推测PPSPⅡ、PPSPⅢ组分抑癌活性可能与其含有β-D-葡萄吡喃聚糖或糖蛋白的结构有关。展开更多
A new binuclear copper ?complex with 1,10-phen and fluoroquinolone ligands, [Cu2(cfc)2(phen)2(pip)](NO3)2(CO)2,was synthesized and its crystal structure was solved.The formula of the complex is C28H24ClCuFN5O7, <IM...A new binuclear copper ?complex with 1,10-phen and fluoroquinolone ligands, [Cu2(cfc)2(phen)2(pip)](NO3)2(CO)2,was synthesized and its crystal structure was solved.The formula of the complex is C28H24ClCuFN5O7, <IMG SRC="IMAGE/09150030. JPG" HEIGHT=11 WIDTH=12>and the crystallography data are as follows, triclinic, space group , a=0.80372(6)nm, b=0.94712 (7)nm, c=1.67826(13)nm, α=88.5650(10)° , β=80.9920(10)°, γ=70.2780(10)°, V=1.18722(6)nm3, Z=2, D=1.848g·cm-3, F(0 00)=676. R1=0.0627 and wR2=0.1684 (I >2σ(I)). The copper atom is five-coordina te with square-based pyramidal geometry and involves two nitrogen atoms from ph en, two oxygen atoms from cfc and one nitrogen atom from pip anion. The stacking effect between cfc and phen ligands from two neighboring molecules was observed . FT-IR spectrum of the complex was also discussed. Both ligand and complex wer e assayed against gram-positive and gram-negative bacteria by doubling dilutio ns method; the complex shows the same minimal inhibitory concentration (MIC) as the corresponding ligand against S. Aureus, E. Coli and M. Lutens bacteria. The inhibitory effect of the ligand and complex on leukemia HL-60 cell line and liv er cancer BEL-7402 cell line have been measured by using MTT (Methyl-Thiazol- Tetrozolium) and SRB (Sulphurhodamin B) assay methods, respectively. The results indicated that the complex has strong inhibitory effect on HL-60 and BEL-7402 cell line. CCDC: 203964.展开更多
文摘IC 50 values of 13 flavonoids to lipid peroxidation induced by OH · in liver microsomer from rat were evaluated and the relationship between structure and activity was studied. The flavonoids with catechol group, which usually have a higher energy in the highest occupied molecular orbital and lower ΔHOF values, have higher inhibitory activities than those without catechol group. The flavonoids without catechol group in B ring not being suitable structure to chelate with Fe 2+ have little inhibitory activities. The results suggest the anti-lipid peroxidation activity of flavonoids is related with the structure of flavonoids.
文摘目的:分离紫心甘薯的多糖(Polysaccharide of purple sweet potato,PPSP)组分,并对其组分的抑癌活性进行初步研究。方法:采用DEAE-Cellulose和CM-Cellulose离子交换柱分离紫心甘薯多糖,收集分离PPSP组分;MTT法检测PPSP各组分对肿瘤细胞的体外抑制活性;薄层层析法分析PPSP各组分的单糖组成;利用傅里叶红外光谱技术检测PPSP组分的结构特征。结果:选用弱阴离子交换柱DEAE-Cellulose分离,并经NaCl溶液梯度分段洗脱,得到4个紫心甘薯多糖组分,分别命名为PPSPⅠ、PPSPⅡ、PPSPⅢ和PPSPⅣ;观察PPSPⅠ、PPSPⅡ、PPSPⅢ对Hela和HepG2肿瘤细胞的抑制作用,检测发现PPSPⅡ、PPSPⅢ组分有一定的抑癌活性。结构分析结果表明,PPSPⅠ主要由葡萄糖和半乳糖这两种单糖组成;PPSPⅡ由葡萄糖组成,并具有β-D-葡萄吡喃聚糖的红外特征吸收峰;PPSPⅢ具有蛋白吸收峰。结论:成功分离得到PPSP的4种多糖组分,其中检测到PPSPⅡ、PPSPⅢ组分对Hela和HepG2肿瘤细胞具有一定的体外抑制作用。推测PPSPⅡ、PPSPⅢ组分抑癌活性可能与其含有β-D-葡萄吡喃聚糖或糖蛋白的结构有关。
文摘A new binuclear copper ?complex with 1,10-phen and fluoroquinolone ligands, [Cu2(cfc)2(phen)2(pip)](NO3)2(CO)2,was synthesized and its crystal structure was solved.The formula of the complex is C28H24ClCuFN5O7, <IMG SRC="IMAGE/09150030. JPG" HEIGHT=11 WIDTH=12>and the crystallography data are as follows, triclinic, space group , a=0.80372(6)nm, b=0.94712 (7)nm, c=1.67826(13)nm, α=88.5650(10)° , β=80.9920(10)°, γ=70.2780(10)°, V=1.18722(6)nm3, Z=2, D=1.848g·cm-3, F(0 00)=676. R1=0.0627 and wR2=0.1684 (I >2σ(I)). The copper atom is five-coordina te with square-based pyramidal geometry and involves two nitrogen atoms from ph en, two oxygen atoms from cfc and one nitrogen atom from pip anion. The stacking effect between cfc and phen ligands from two neighboring molecules was observed . FT-IR spectrum of the complex was also discussed. Both ligand and complex wer e assayed against gram-positive and gram-negative bacteria by doubling dilutio ns method; the complex shows the same minimal inhibitory concentration (MIC) as the corresponding ligand against S. Aureus, E. Coli and M. Lutens bacteria. The inhibitory effect of the ligand and complex on leukemia HL-60 cell line and liv er cancer BEL-7402 cell line have been measured by using MTT (Methyl-Thiazol- Tetrozolium) and SRB (Sulphurhodamin B) assay methods, respectively. The results indicated that the complex has strong inhibitory effect on HL-60 and BEL-7402 cell line. CCDC: 203964.
