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光合细菌类胡萝卜素的提取工艺优化及抗氧化活性研究 被引量:4
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作者 李尽哲 王伟 黄雅琴 《中国食品添加剂》 CAS 北大核心 2016年第6期80-86,共7页
为了建立一套相对简单、操作性强、提取率高的光合细菌类胡萝卜素提取工艺,利用单因素和正交试验优化了光合细菌类胡萝卜素的提取工艺。通过超声波辅助提取,分别考察了不同有机溶剂、超声时间、浸提时间、料液比4个因素对光合细菌类胡... 为了建立一套相对简单、操作性强、提取率高的光合细菌类胡萝卜素提取工艺,利用单因素和正交试验优化了光合细菌类胡萝卜素的提取工艺。通过超声波辅助提取,分别考察了不同有机溶剂、超声时间、浸提时间、料液比4个因素对光合细菌类胡萝卜素的提取效果,最终获得的最佳提取条件为丙酮石油醚混合液(体积比1∶1)为提取溶剂,超声时间30 min,浸提时间4.5 h,料液比是0.1(g)/6.5(mL)。在该条件下提取,类胡萝卜素的提取量达到9432.56μg/g。通过不同浓度的类胡萝卜素及不同抗氧化剂对猪油POV值的影响试验,证明光合细菌类胡萝卜素具有较强的抗氧化活性,是一种来源于光合细菌、抗氧化效果较好的天然色素类食品添加剂。 展开更多
关键词 光合细菌 类胡萝卜素 提取工艺 优化 抗氧化活性
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乳酸菌对苜蓿青贮发酵品质和细菌菌群的影响 被引量:5
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作者 王媛 王雁萍 +3 位作者 赵珊珊 杨逢源 郝湘妹 范小苗 《中国饲料》 北大核心 2021年第5期36-39,共4页
为探究乳酸菌作为青贮添加剂对苜蓿青贮发酵品质和细菌菌群的影响,试验选取萎焉苜蓿为原料分为三个处理组,分别为无添加剂对照组(CK组)和分别添加1×106cfu/g乳酸菌YX(LPI组)、乳酸菌ZZU A341(LPA组)。结果表明:LPI或LPA组乳酸积累... 为探究乳酸菌作为青贮添加剂对苜蓿青贮发酵品质和细菌菌群的影响,试验选取萎焉苜蓿为原料分为三个处理组,分别为无添加剂对照组(CK组)和分别添加1×106cfu/g乳酸菌YX(LPI组)、乳酸菌ZZU A341(LPA组)。结果表明:LPI或LPA组乳酸积累量显著高于CK组(P<0.05),pH和氨态氮含量显著低于CK组(P<0.05),且pH均降至5.00以下;CK组或LPI组的优势菌属为Pediococcus属(56.40%、98.63%),LPA组为Lactobacillus属(98.88%)。综上所述,乳酸菌作为青贮添加剂可通过调控青贮环境中细菌菌群的组成或相对丰度,从而提高青贮苜蓿的发酵品质。 展开更多
关键词 苜蓿青贮 细菌菌群 高通量测序 乳酸菌
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Rapid Identification for Drought Resistance of Wheat Using Near-infrared Diffuse Reflectance Spectroscopy 被引量:3
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作者 冯伟森 吴少辉 +7 位作者 谷运红 张园 高海涛 王卫东 张灵帅 张学品 马飞 张灿军 《Agricultural Science & Technology》 CAS 2012年第12期2615-2619,共5页
[Objective] This study aimed to establish an identification system for drought-resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] In 2006-2007, 36 wheat varieties with different drou... [Objective] This study aimed to establish an identification system for drought-resistance in wheat by using near-infrared diffuse reflectance spectroscopy. [Method] In 2006-2007, 36 wheat varieties with different drought resistance were selected and were classified according to their drought resistance grades determined by the Technical Specification of Identification and Evaluation for Drought Resistance in Wheat (GB/T 21127-2007). In addition, the harvested wheat seed samples were spectrally analyzed with FOSS NIRSystems5000 near-infrared spectrum analyzer for grain quality (full spectrum analyzer) and then the forecasted regression equations were established. [Result] After the establishment of a database and validation, dis- criminated functions were obtained. The determination coefficient (RSQ) and coeffi- cients of determination for cross validation (1-VR) in the discriminant function built with seed samples from water stress area were 0.846 0 and 0.781 8, respectively, which indicated that the consistency between drought resistance and spectral charac- teristics in wheat varieties was good, and there was high correlation between the near-infrared diffuse reflectance spectra of seeds and the drought resistance in wheat. [Conclusiou] Under water stress condition, it is feasible to establish a conve- nient, rapid and no-damage identification system for the drought resistance in wheat by using the near-infrared diffuse reflectance spectrum technique to scan wheat seeds. 展开更多
关键词 Near-infrared diffuse reflectance spectroscopy Identification for wheat drought resistance Discriminant function
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