目的探究内皮素3(EDN3)、黑色素瘤优先表达抗原(PRAME)表达水平与子宫内膜癌临床特征及预后的关系。方法选取郑州大学第二附属医院2018年9月至2020年9月收治的80例子宫内膜癌患者为研究对象,在术中收集癌组织和癌旁组织;采用定量反转录...目的探究内皮素3(EDN3)、黑色素瘤优先表达抗原(PRAME)表达水平与子宫内膜癌临床特征及预后的关系。方法选取郑州大学第二附属医院2018年9月至2020年9月收治的80例子宫内膜癌患者为研究对象,在术中收集癌组织和癌旁组织;采用定量反转录聚合酶链反应(qRT-PCR)和免疫组化法检测EDN3、PRAME表达;EDN3、PRAME与子宫内膜癌患者预后的关系采用Kaplan-Meier生存曲线分析;子宫内膜癌患者预后的影响因素采用COX回归分析。绘制受试者操作特征曲线分析EDN3、PRAME对子宫内膜癌预后不良的预测价值。结果子宫内膜癌组织EDN3信使核糖核酸(mRNA)表达水平和阳性表达率显著低于癌旁组织(P<0.05),PRAME mRNA表达水平和阳性表达率显著高于癌旁组织(P<0.05)。子宫内膜癌组织中EDN3、PRAME表达与国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)分期、分化程度、淋巴结转移有关(P<0.05)。EDN3阳性患者生存率高于阴性患者(P<0.05);PRAME阳性患者生存率低于阴性患者(P<0.05)。预后不良组患者EDN3 mRNA表达水平显著低于预后良好组,PRAME mRNA表达水平显著高于预后良好组(P<0.05)。根据受试者操作特征曲线得知,EDN3和PRAME二者联合预测子宫内膜癌预后不良优于各自单独预测(Z_(联合vsEDN3)=2.667、Z_(联合vsPRAME)=2.682,P<0.05)。多因素COX回归分析结果显示,PRAME、FIGO分期、分化程度、淋巴结转移是影响子宫内膜癌患者预后的危险因素(P<0.05),EDN3是保护因素(P<0.05)。结论子宫内膜癌组织中EDN3表达显著降低,PRAME表达显著升高,二者与临床特征及预后密切相关。展开更多
目的:探讨阴道微生态指标与HR-HPV阳性上皮内瘤变(CIN)的相关性。方法:选取HR-HPV阳性且宫颈活检病理提示CIN的215例患者为观察组,病理提示宫颈良性病变的333例患者为对照组。将两组一般情况、阴道微生态指标采用卡方检验或Mann-Whitney...目的:探讨阴道微生态指标与HR-HPV阳性上皮内瘤变(CIN)的相关性。方法:选取HR-HPV阳性且宫颈活检病理提示CIN的215例患者为观察组,病理提示宫颈良性病变的333例患者为对照组。将两组一般情况、阴道微生态指标采用卡方检验或Mann-WhitneyU检验进行单因素分析,将有统计差异的年龄、HPV分型、清洁度、白细胞计数异常率、LE、SNa、NAG、BV进行二元Logistic多因素回归,分析HR-HPV阳性CIN发生的独立危险因素。结果:与年龄 ≤ 25岁相比,年龄在26~45、46~65岁的患者HR-HPV感染均是CIN发生的保护因素[B = −0.821, OR (95%CI): 0.44 (0.259~0.746), B = −3.399, OR (95%CI): 0.033 (0.012~0.094)];与HR-HPV单一型相比,HR-HPV混合型是CIN的危险因素[(B = 0.459, OR (95%CI): 1.583 (1.017~2.464)];BV(+)、LE(+)是CIN发生的独立危险因素[(B = 4.436, OR (95%CI): 84.457 (8.485~840.682), B = 0.519, OR (95%CI): 1.68 (1.049~2.692)]。结论:阴道微生态与HR-HPV阳性CIN有相关性,阴道微生态失衡可能参与宫颈病变的发生。Aim: To investigate the correlation between vaginal microecological indicators and HR-HPV-positive intraepithelial neoplasia (CIN). Methods: 215 patients with HR-HPV-positive cervical biopsy indicating CIN were selected as the observation group, and 333 patients with cervical benign lesions were selected as the control group. Chi-square test or Mann-WhitneyU test was used for univariate analysis of the general conditions and vaginal microecological indicators of the two groups, and binary Logistic multivariate regression was performed for age, HPV type, cleanliness, abnormal rate of white blood cell count, LE, SNa, NAG and BV with statistical differences. Independent risk factors for HR-HPV-positive CIN were analyzed. Results: Compared with patients aged ≤ 25 years, HR-HPV infection in patients aged 26~45 and 46~65 years was a protective factor for CIN [B = −0.821, OR (95%CI): 0.44 (0.259~0.746), B = −3.399, OR (95%CI): 0.033 (0.012~0.094)];compared with HR-HPV single type, HR-HPV mixed type was a risk factor for CIN [(B = 0.459, OR (95%CI): 1.583 (1.017~2.464)]. BV(+) and LE(+) were independent risk factors for CIN [(B = 4.436, OR (95%CI): 84.457 (8.485~840.682), B = 0.519, OR (95%CI): 1.68 (1.049~2.692)]. Conclusion: Vaginal microecology is correlated with HR-HPV-positive CIN, and the imbalance of vaginal microecology may be involved in the occurrence of cervical lesions.展开更多
目的:探讨MARCH2和CFTR在结直肠癌中的蛋白表达及与临床病理参数的相关性。方法:采用免疫组织化学SP法检测125例结直肠癌和30例正常结直肠黏膜组织中MARCH2和CFTR的表达,并复习相关文献。结果:在125例结直肠癌组织中,MARCH2和CFTR的阳...目的:探讨MARCH2和CFTR在结直肠癌中的蛋白表达及与临床病理参数的相关性。方法:采用免疫组织化学SP法检测125例结直肠癌和30例正常结直肠黏膜组织中MARCH2和CFTR的表达,并复习相关文献。结果:在125例结直肠癌组织中,MARCH2和CFTR的阳性表达率分别为84.8%和8.8%,而在非肿瘤性肠组织中,这两种表达率的阳性率分别为6.7%和90.0%。MARCH2的阳性表达与肿瘤大小、深部浸润、淋巴结转移和TNM分期密切相关。此外,CFTR表达的缺失与分化不良、淋巴结转移和TNM分期显著相关。此外,MARCH2和CFTR表达之间存在显著负相关。结论:MARCH2在结直肠癌中高度表达,与肿瘤大小、深部浸润、淋巴结转移和TNM分期等预后不良参数有关。相比之下,CFTR在结直肠癌中几乎不表达,CFTR表达缺失与分化不良、淋巴结转移和TNM分期显著相关。MARCH2可能通过与CFTR的相互作用在结直肠癌的发展中发挥重要作用。它们可能成为结直肠癌诊断、治疗和预后评估的分子标志物。Aims: This study aimed to investigate whether the expressions of MARCH2 and CFTR in colorectal carcinomas were associated with clinicopathological parameters. Methods: The expressions of MARCH2 and CFTR were examined in 125 cases of colorectal carcinomas and 30 normal colorectal tissues using immunohistochemical staining. Results: The positive rates of MARCH2 and CFTR expressions in 125 cases of colorectal carcinoma were 84.8% and 8.8% respectively, whereas the positive expression rates of MARCH2 and CFTR in non-tumor colorectal tissues were 6.7% and 90.0% respectively. The positive expressions of MARCH2 were closely related to tumor size, deep invasion, lymph node metastasis and advanced TNM stage. In addition, loss of expression of CFTR was significantly associated with poor differentiation, lymph node metastasis and advanced TNM stage. Furthermore, there were significant negative correlations between MARCH2 and CFTR expression. Conclusions: MARCH2 is highly expressed in colorectal carcinomas, and is associated with poor prognostic parameters such as tumor size, deep invasion, lymph node metastasis and advanced TNM stage. In contrast, CFTR barely expressed in colorectal carcinomas, and loss of expression of CFTR was significantly associated with poor differentiation, lymph node metastasis and advanced TNM stage. MARCH2 may play an important role in the development of colorectal carcinoma through interaction with CFTR. They may become molecular markers for diagnosis, treatment and prognostic evaluation of colorectal carcinomas.展开更多
文摘目的探究内皮素3(EDN3)、黑色素瘤优先表达抗原(PRAME)表达水平与子宫内膜癌临床特征及预后的关系。方法选取郑州大学第二附属医院2018年9月至2020年9月收治的80例子宫内膜癌患者为研究对象,在术中收集癌组织和癌旁组织;采用定量反转录聚合酶链反应(qRT-PCR)和免疫组化法检测EDN3、PRAME表达;EDN3、PRAME与子宫内膜癌患者预后的关系采用Kaplan-Meier生存曲线分析;子宫内膜癌患者预后的影响因素采用COX回归分析。绘制受试者操作特征曲线分析EDN3、PRAME对子宫内膜癌预后不良的预测价值。结果子宫内膜癌组织EDN3信使核糖核酸(mRNA)表达水平和阳性表达率显著低于癌旁组织(P<0.05),PRAME mRNA表达水平和阳性表达率显著高于癌旁组织(P<0.05)。子宫内膜癌组织中EDN3、PRAME表达与国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)分期、分化程度、淋巴结转移有关(P<0.05)。EDN3阳性患者生存率高于阴性患者(P<0.05);PRAME阳性患者生存率低于阴性患者(P<0.05)。预后不良组患者EDN3 mRNA表达水平显著低于预后良好组,PRAME mRNA表达水平显著高于预后良好组(P<0.05)。根据受试者操作特征曲线得知,EDN3和PRAME二者联合预测子宫内膜癌预后不良优于各自单独预测(Z_(联合vsEDN3)=2.667、Z_(联合vsPRAME)=2.682,P<0.05)。多因素COX回归分析结果显示,PRAME、FIGO分期、分化程度、淋巴结转移是影响子宫内膜癌患者预后的危险因素(P<0.05),EDN3是保护因素(P<0.05)。结论子宫内膜癌组织中EDN3表达显著降低,PRAME表达显著升高,二者与临床特征及预后密切相关。
文摘目的:探讨阴道微生态指标与HR-HPV阳性上皮内瘤变(CIN)的相关性。方法:选取HR-HPV阳性且宫颈活检病理提示CIN的215例患者为观察组,病理提示宫颈良性病变的333例患者为对照组。将两组一般情况、阴道微生态指标采用卡方检验或Mann-WhitneyU检验进行单因素分析,将有统计差异的年龄、HPV分型、清洁度、白细胞计数异常率、LE、SNa、NAG、BV进行二元Logistic多因素回归,分析HR-HPV阳性CIN发生的独立危险因素。结果:与年龄 ≤ 25岁相比,年龄在26~45、46~65岁的患者HR-HPV感染均是CIN发生的保护因素[B = −0.821, OR (95%CI): 0.44 (0.259~0.746), B = −3.399, OR (95%CI): 0.033 (0.012~0.094)];与HR-HPV单一型相比,HR-HPV混合型是CIN的危险因素[(B = 0.459, OR (95%CI): 1.583 (1.017~2.464)];BV(+)、LE(+)是CIN发生的独立危险因素[(B = 4.436, OR (95%CI): 84.457 (8.485~840.682), B = 0.519, OR (95%CI): 1.68 (1.049~2.692)]。结论:阴道微生态与HR-HPV阳性CIN有相关性,阴道微生态失衡可能参与宫颈病变的发生。Aim: To investigate the correlation between vaginal microecological indicators and HR-HPV-positive intraepithelial neoplasia (CIN). Methods: 215 patients with HR-HPV-positive cervical biopsy indicating CIN were selected as the observation group, and 333 patients with cervical benign lesions were selected as the control group. Chi-square test or Mann-WhitneyU test was used for univariate analysis of the general conditions and vaginal microecological indicators of the two groups, and binary Logistic multivariate regression was performed for age, HPV type, cleanliness, abnormal rate of white blood cell count, LE, SNa, NAG and BV with statistical differences. Independent risk factors for HR-HPV-positive CIN were analyzed. Results: Compared with patients aged ≤ 25 years, HR-HPV infection in patients aged 26~45 and 46~65 years was a protective factor for CIN [B = −0.821, OR (95%CI): 0.44 (0.259~0.746), B = −3.399, OR (95%CI): 0.033 (0.012~0.094)];compared with HR-HPV single type, HR-HPV mixed type was a risk factor for CIN [(B = 0.459, OR (95%CI): 1.583 (1.017~2.464)]. BV(+) and LE(+) were independent risk factors for CIN [(B = 4.436, OR (95%CI): 84.457 (8.485~840.682), B = 0.519, OR (95%CI): 1.68 (1.049~2.692)]. Conclusion: Vaginal microecology is correlated with HR-HPV-positive CIN, and the imbalance of vaginal microecology may be involved in the occurrence of cervical lesions.
文摘目的:探讨MARCH2和CFTR在结直肠癌中的蛋白表达及与临床病理参数的相关性。方法:采用免疫组织化学SP法检测125例结直肠癌和30例正常结直肠黏膜组织中MARCH2和CFTR的表达,并复习相关文献。结果:在125例结直肠癌组织中,MARCH2和CFTR的阳性表达率分别为84.8%和8.8%,而在非肿瘤性肠组织中,这两种表达率的阳性率分别为6.7%和90.0%。MARCH2的阳性表达与肿瘤大小、深部浸润、淋巴结转移和TNM分期密切相关。此外,CFTR表达的缺失与分化不良、淋巴结转移和TNM分期显著相关。此外,MARCH2和CFTR表达之间存在显著负相关。结论:MARCH2在结直肠癌中高度表达,与肿瘤大小、深部浸润、淋巴结转移和TNM分期等预后不良参数有关。相比之下,CFTR在结直肠癌中几乎不表达,CFTR表达缺失与分化不良、淋巴结转移和TNM分期显著相关。MARCH2可能通过与CFTR的相互作用在结直肠癌的发展中发挥重要作用。它们可能成为结直肠癌诊断、治疗和预后评估的分子标志物。Aims: This study aimed to investigate whether the expressions of MARCH2 and CFTR in colorectal carcinomas were associated with clinicopathological parameters. Methods: The expressions of MARCH2 and CFTR were examined in 125 cases of colorectal carcinomas and 30 normal colorectal tissues using immunohistochemical staining. Results: The positive rates of MARCH2 and CFTR expressions in 125 cases of colorectal carcinoma were 84.8% and 8.8% respectively, whereas the positive expression rates of MARCH2 and CFTR in non-tumor colorectal tissues were 6.7% and 90.0% respectively. The positive expressions of MARCH2 were closely related to tumor size, deep invasion, lymph node metastasis and advanced TNM stage. In addition, loss of expression of CFTR was significantly associated with poor differentiation, lymph node metastasis and advanced TNM stage. Furthermore, there were significant negative correlations between MARCH2 and CFTR expression. Conclusions: MARCH2 is highly expressed in colorectal carcinomas, and is associated with poor prognostic parameters such as tumor size, deep invasion, lymph node metastasis and advanced TNM stage. In contrast, CFTR barely expressed in colorectal carcinomas, and loss of expression of CFTR was significantly associated with poor differentiation, lymph node metastasis and advanced TNM stage. MARCH2 may play an important role in the development of colorectal carcinoma through interaction with CFTR. They may become molecular markers for diagnosis, treatment and prognostic evaluation of colorectal carcinomas.
