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云南木霉Trichoderma yunnanense不同处理发酵液对茶炭疽菌抑制活性的稳定性
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作者 李群 冯月玲 +2 位作者 王梅 吴元竞 刘辉 《农技服务》 2024年第9期55-61,共7页
为云南木霉在生物农药中的开发应用提供参考,在发酵条件为甘露醇18.85 g/L、酵母浸膏4.73 g/L、装液量93.15 mL/250 mL,发酵温度25℃、12 h光照/12 h黑暗、pH 6.6、转速150 r/min,发酵天数5 d的情况下,研究传代次数、金属元素、pH、温... 为云南木霉在生物农药中的开发应用提供参考,在发酵条件为甘露醇18.85 g/L、酵母浸膏4.73 g/L、装液量93.15 mL/250 mL,发酵温度25℃、12 h光照/12 h黑暗、pH 6.6、转速150 r/min,发酵天数5 d的情况下,研究传代次数、金属元素、pH、温度、储藏时间、氧化还原剂等对云南木霉T.yunnanense发酵液的茶炭疽菌抑菌活性的影响。结果表明:云南木霉可稳定传代10次,其抑菌率可维持在85%以上,在Ca^(2+)、Zn^(2+)、Fe^(3+)等金属离子、pH为2.32~12.03、温度为-4℃及25℃储藏5~40 d、不同浓度氧化还原剂等条件下对云南木霉发酵液的抑菌活性影响不显著,其抑菌率均可维持在85%以上。云南木霉T.yunnanense的环境适应性较好,可在极端环境下维持较高的茶炭疽菌抑菌效果,具有较好的应用价值。 展开更多
关键词 木霉菌 Trichoderma yunnanense 发酵稳定性 茶炭疽病 生物防治
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干季中云南松对纵坑切梢小蠹伴生菌(Leptographium yunnanense)抗性的变化 被引量:10
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作者 廖周瑜 叶辉 +1 位作者 吕军 林雁 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2004年第9期84-88,共5页
利用云南半帚孢测试了干季中云南松抗性的变化规律.结果表明,云南松抗性的相对强弱与降雨量及环境温度均表现出一定程度的负相关关系,相关系数分别为r=-0.779 8和r=-0.462 7,|r0.05|=0.811,P>0.05.在干季前期,温度较低,降雨量偏少,... 利用云南半帚孢测试了干季中云南松抗性的变化规律.结果表明,云南松抗性的相对强弱与降雨量及环境温度均表现出一定程度的负相关关系,相关系数分别为r=-0.779 8和r=-0.462 7,|r0.05|=0.811,P>0.05.在干季前期,温度较低,降雨量偏少,松树的针叶及韧皮含水量较低,伴生菌的生长力较低,云南松的抗性相对增强,这一时期内纵坑切梢小蠹危害较轻;而干季后期,温度逐渐升高,降雨量也有所增加,松树含水量较高,伴生菌的生长力增强,云南松的抗性相对减弱,因此该时期内纵坑切梢小蠹的蛀干危害较严重. 展开更多
关键词 云南松 纵坑切梢小蠹 云南半帚孢(Leptographiurn yunnanense) 抗性变化
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Mutation Classification and Genetic Diversity Analysis of Triticum aestivum ssp. yunnanense King Based on Agronomic Traits
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作者 王志伟 杨金华 +3 位作者 程加省 胡银星 程耿 于亚雄 《Agricultural Science & Technology》 CAS 2010年第11期119-123,共5页
[Objective] The present study was conducted to provide basis for researches on breeding new wheat varieties and conserving rare species in Yunnan Province.[Method] 29 materials of Triticum aestivum ssp.yunnanense King... [Objective] The present study was conducted to provide basis for researches on breeding new wheat varieties and conserving rare species in Yunnan Province.[Method] 29 materials of Triticum aestivum ssp.yunnanense King(Yunnan Hulled Wheat)were taken for the research on classification and genetic diversity analysis based on 14 agronomic traits.[Result] Yunnan hulled wheat A14 could be an unnamed white seed variety,and other 28 materials were divided into 10 named mutation types;Yunnan hulled wheat was rich in variability of agronomic traits,in which the coefficient of variance(CV)of sterile spikelet number was the largest(22.59%),while the CV of earing period was the lowest(3.71%);the diversity indexes of seven qualitative characters ranged from 1.55-2.04.Moreover,the 29 Yunnan hulled wheat could be divided into 3 clusters by the UPGMA analysis.However,Yunnan hulled wheat varieties originating from similar mutation types did not completely cluster together.The genetic relationship was relatively close among A13(YT-35),A14(YT-36)and A21(YT-37),but the faster genetic relationship was observed between them and other Yunnan hulled wheat varieties.[Conclusion] Yunnan hulled wheat had an extensive genetic diversity in agronomic traits. 展开更多
关键词 Triticum aestivum ssp.yunnanense King(Yunnan hulled wheat) Agronomic traits Genetic diversity
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云南杜鹃(Rhododendron yunnanense Franch.)无菌叶片高频再生体系的建立 被引量:7
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作者 汪玲敏 李世峰 +4 位作者 解玮佳 王继华 关文灵 宋杰 彭绿春 《分子植物育种》 CAS CSCD 北大核心 2015年第8期1877-1883,共7页
本研究以云南杜鹃(Rhododendron yunnanense Franch.)无菌苗叶片为材料,研究云南杜鹃叶片直接和间接再生植株的方法。结果表明:ZT是理想的云南杜鹃无菌叶片直接分化不定芽和愈伤组织分化不定芽的细胞分裂素,其中以WPM+ZT 4 mg/L+NAA 0.0... 本研究以云南杜鹃(Rhododendron yunnanense Franch.)无菌苗叶片为材料,研究云南杜鹃叶片直接和间接再生植株的方法。结果表明:ZT是理想的云南杜鹃无菌叶片直接分化不定芽和愈伤组织分化不定芽的细胞分裂素,其中以WPM+ZT 4 mg/L+NAA 0.01 mg/L效果最好,叶片分化不定芽率达74%,平均分化不定芽数6.91个,愈伤组织分化不定芽率达76.67%;附加TDZ诱导出的愈伤组织状态好,体积大,疏松,适宜的叶片诱导愈伤组织的培养基为WPM+TDZ 0.5mg/L+2,4-D 1~2 mg/L+水解酪蛋白500 mg/L,愈伤组织诱导率达91.33%以上,较好的生根培养基为1/2 WPM+IAA 1.5 mg/L,生根率达89.33%以上。研究结果为云南杜鹃遗传转化研究奠定了基础。 展开更多
关键词 云南杜鹃(Rhododendron yunnanense) 无菌叶片 高频再生
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Three new species of Batrachospermum Roth (Batrachosper-maceae,Rhodophyta) in China 被引量:5
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作者 谢树莲 施之新 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2005年第2期204-209,共6页
Three new species of Batrachospermum Roth (Batrachospermales, Rhodophyta) from China are described in this paper. B. yunnanense sp. nov. has long-cylindrical trichogynes with long stalks and is diagnostic of section V... Three new species of Batrachospermum Roth (Batrachospermales, Rhodophyta) from China are described in this paper. B. yunnanense sp. nov. has long-cylindrical trichogynes with long stalks and is diagnostic of section Virescentia. Within this section, B. yunnanense is similar to B. helminthosum Bory emend. Sheath et al., but it is dioecious and has curved carpogonial branches, while the latter is monoecious and has straight carpogonial branches. It is also similar to B. transtaganum Reis, but it differs from the latter in long carpogonia, big carposporophytes and carposporangia. It is considered that B. nothocladoideum sp. nov. is assigned to section Contorta, subsection Kushiroense, because its carpogonial branches are twisted and gonimoblast filaments are loosely agglomerated. This new species similar to B. iriomotense Kumano, but with short fascicles, long-ovoid or subpyriform cells, numerous terminal hairs, long-ellipsoid trichogynes, big carposporophytes and small carpo- sporangia. The plant is quite tough and cartilaginous and similar to Nothocladus in gross morphology, but its carposporophytes are compact instead of diffuse. This shows that it may be a transitional species between section Contorta and genus Nothocladus. So, B. transitorium sp. nov. should belong to section Contorta, subsection Kushiroense, because of its curved or twisted carpogonial branches and loosely agglomerated gonimoblast filaments, with globose or subglobose cells in fascicles similar to B. spermatiophorum Vis et Sheath, but no colourless spermatiophores. In terms of small and numerous carposporophytes, B. transitorium sp. nov. is similar to some species of section Batrachospermum. However, their other features are unique, indicating its transitional nature between section Contorta and Batrachospermum. 展开更多
关键词 freshwater red algae Batrachospermum B. yunnanense S. L. Xie et Z. X. Shi B. nothocladoideum S. L. Xie et Z. X. Shi B. transitorium S. L. Xie et Z. X. Shi new species China
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The Reduction of a Natural Diterpene Containing a, B-Unsaturated Keto Group
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作者 QingZHAO XinHONG +6 位作者 ChengZOU YueMaoSHEN XiaoJiangHAO QingZHAO XinHONG YueMaoSHEN XiaoJiangHAO 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第10期1015-1017,共3页
Hedychenone (1), a diterpene containing a, B-unsaturated keto group, was reduced by aluminum-mercury alloy, and a dimerized product (2) was obtained as the major product. The coupling occurred at B position of the ket... Hedychenone (1), a diterpene containing a, B-unsaturated keto group, was reduced by aluminum-mercury alloy, and a dimerized product (2) was obtained as the major product. The coupling occurred at B position of the keto group. 展开更多
关键词 Hedychenone Hedychium yunnanense DITERPENE reduction.
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Allelopathy of Gaseous Volatiles of Eupatorium adenophorum on Seed Germination and Seedling Growth of Pinus yunanensis 被引量:1
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作者 Cao Zilin Wang Xiaoli +3 位作者 Li Genqian Li Xiaoting Gao Hui Yuan Sian 《Plant Diseases and Pests》 CAS 2013年第4期1-4,16,共5页
[ Objective] The paper was to understand allelopathy of gaseous volatiles of Eupatorium adenophorum stems and leaves on seed germination and seedling growth of P/nus yunnanens/s. [ Method] P. yunnanens/s seeds were tr... [ Objective] The paper was to understand allelopathy of gaseous volatiles of Eupatorium adenophorum stems and leaves on seed germination and seedling growth of P/nus yunnanens/s. [ Method] P. yunnanens/s seeds were treated by fresh stems and leaves of E. adenophorum in translucent airtight container under high concentration (0.069 4 g/cm3 ), middle concentration (0.023 1 g/cm3 ) and lower concentration (0. 007 7 g/cm3 ), respectively. [ Result] Gaseous volatiles affected seed germination process; with the increasing processing concentration, the day on which seeds began to germinate and the day reaching germination peak were delayed, and the germination quantity on the day reaching germination peak was decreased ; the day on which the germination quantity began to significantly re- duce was delayed ; the process curve was developed from sudden increase and sudden decrease to gentle change, and nearly became a beeline at high concentration. The effects of gaseous volatiles on seed germination ( seed germination rate, germination potential, germination index) and seedling growth (fresh weight, dry weight, radicle length, hypceotyl length) of P. yunnanens/s were basically consistent, showing a regularity that high concentration had stronger inhibition effect and the inhibition effect became weaker with the decreasing concentration of E. adenophorum. E. adenophorum was widely distributed under P. yunnanens/s forests, and might affect natural regeneration and growth of P. yunnanens/s via allelopathy. [ Conclusion ] The paper provides reference for scientific management of P. yunnanens/s forests. 展开更多
关键词 Eupator/um adenophorum Gaseous volatiles Pinus yunnanens/s Seed germination Seedling growth ALLELOPATHY
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Comparative authentication of crude drugs from subgenus Asarum species in China based on microscopic features
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作者 Yaoli Li Zhong Liu +4 位作者 Jie Yu Mingying Shang Baibo Xie Feng Xu Shao-Qing Cai 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2020年第12期855-867,共13页
According to the Chinese Pharmacopoeia,the Chinese medicinal material(Asari Radix et Rhizoma)Xixin is derived from the species Asarum heterotropoides var.mandshuricum,A.sieboldii,and A.sieboldii var.seoulense.However,... According to the Chinese Pharmacopoeia,the Chinese medicinal material(Asari Radix et Rhizoma)Xixin is derived from the species Asarum heterotropoides var.mandshuricum,A.sieboldii,and A.sieboldii var.seoulense.However,many species of the genus Asarum are labeled as"Xixin","Tu-Xixin",or"Nan-Xixin"for sale in producing areas.To distinguish Chinese Asarum species and to ensure their safety and efficacy,we examined the microscopic characteristics of subg.Asarum plants in China(including subg.Asarum plants in the classification system of Flora Reipublicae Popularis Sinicae and the later published Asarum yunnanense)using a hand microtome and a microscopic digital imaging system based on our previous research.Microscopic features of the blades,petioles,and roots of A.yunnanense were investigated for the first time.Moreover,we compared nine species and one variety of the subg.Asarum(i.e.,A.yunnanense,A.caudigerellum,A.himalaicum,A.pulchellum,A.debile,A.caulescens,A.renicordatum,A.caudigerum,A.caudigerum var.cardiophyllum and A.geophilum)in China based on microscopic characteristics of the blades.Several parameters,such as morphological characteristics and distribution of hairs on both upper and lower leaf epidermises;morphological characteristics of hair cells;anticlinal walls of lower epidermal cells;size relationship between outer periclinal walls and cavity outlines of oil cells on lower leaf epidermis;presence or absence of the irregular cells containing red to brown substances among mesophyll cells;and the diameter of the largest vessels in midribs of the blades,were used for discrimination among species.To the best of our knowledge,we provided the first identification key for the 10 taxa based on blade microscopic characteristics that could be used to distinguish these taxa.Based on the key,23 commercial samples collected from seven provinces of China were authenticated as whole dried herbs of A.himalaicum,A.caudigerellum,A.caudigerum,A.pulchellum and A.caulescens.These findings indicated that microscopic approach and related techniques were convenient and feasible and could be used for the authentication of crude drugs from Chinese subg.Asarum species. 展开更多
关键词 AUTHENTICATION Microscopic characteristics Xixin Subg.Asarum Asarum yunnanense
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