[Objective] The paper was to understand the occurrence of gray mold of protected tomato and resistance of introduced tomato varieties to gray mold in Shihezi area. [Method] The occurrence status of gray mold in protec...[Objective] The paper was to understand the occurrence of gray mold of protected tomato and resistance of introduced tomato varieties to gray mold in Shihezi area. [Method] The occurrence status of gray mold in protected tomato in Shawan County, Manas County, the experimental field of Shihezi University and Shihezi Huayu base were investigated during the occurrence period of tomato gray mold, and the resistance of intro-duced variety against tomato gray mold was identified at seedling stage through indoor artificial inoculation method. [Result] The diseased leaf rate of tomato gray mold was 17.52%-100%, the diseased fruit rate was 3.1%-55.69%, and the disease index was 15.98-100. In resistance identifica-tion of different tomato varieties, the disease indexes of Kenfan25, Shifan28, Shifan15, KENFAN-3, KENFAN-5, Shifan35, KENFAN-2 and Shifan-9 were 1.03, 3.1, 1.17, 4.3, 5.32, 5.12, 4.32 and 4.11, respectively. From September to March of the following year, tomato gray mold occurred and was prevalent in greenhouses in Shihezi area. [Conclusion] Shifan28, Kenfan25 and Shifan15 showed resistance to tomato gray mold, while the other five varieties were susceptible to the disease.展开更多
This study addresses the challenge posed by the small spore size of tomato gray mold,which hinders its identification and enumeration by conventional techniques.This work presents a novel approach for quantifying spor...This study addresses the challenge posed by the small spore size of tomato gray mold,which hinders its identification and enumeration by conventional techniques.This work presents a novel approach for quantifying spore counts of tomato gray mold using diffraction imaging technology and image processing techniques.To construct a device for acquiring diffraction images of tomato gray mold spores,initially,the hyperspectral data pertaining to the gray mold spores of tomatoes was obtained.The characteristic wavelength of the light source of the diffraction image acquisition device was obtained by smoothing,principal component analysis,and comprehensive coefficient weight calculation.Then,the key parameters of the system were simulated,and the diffraction image acquisition device was built.Finally,tomato gray mold spores were counted based on angular spectrum reconstruction and image processing.The findings indicated that the combined contribution rate of the initial and secondary principal components of the original spectral data obtained from tomato gray mold spore samples amounted to 92.271%.The visible range of 435 nm,475 nm,and 720 nm can be selected as the light source for tomato gray mold’s spore diffraction imaging system.CMOS image sensor was installed 45 mm below the micropore with a diameter of 100μm,and the diffraction image obtained by simulation has a clear diffraction fingerprint.The diffraction imaging system can collect diffraction images of disease spores,and the collected diffraction images have clear diffraction fingerprints.The experimental error range was 5.13%-8.57%,and the average error was 6.42%.The error was within a 95%consistency.Therefore,this study can provide a research basis for the classification and recognition of greenhouse disease spores.展开更多
The growth rate method was adopted to measure the inhibitory effect of curcumin,tetrahydrocurcumin,demethoxycurcumin,and bisdemethoxycurcumin on the mycelial growth of Botrytis cinerea and Colletotrichum gloeosporioid...The growth rate method was adopted to measure the inhibitory effect of curcumin,tetrahydrocurcumin,demethoxycurcumin,and bisdemethoxycurcumin on the mycelial growth of Botrytis cinerea and Colletotrichum gloeosporioides.The results showed that the four curcuminoids inhibited the mycelial growth of the two pathogens in a concentration-dependent manner.Bisdemethoxycurcumin at 600 mg/L exerted the strongest inhibitory effect on the mycelial growth of B.cinerea and C.gloeosporioides,with the relative inhibition rates of 98.19%and 100%,respectively;followed by demethoxycurcumin;curcumin exerted the worst inhibitory effect.Toxicity test results also showed that four curcuminoids all had a certain toxicity to B.cinerea and C.gloeosporioides,among which,bisdemethoxycurcumin exhibited the strongest toxicity,with the EC_(50)of 131.125 and 122.235 mg/L,respectively;while curcumin had the lowest toxicity,with the EC_(50)of 273.143 and 194.943 mg/L,respectively.展开更多
Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential st...Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.展开更多
基金Supported by Scientific and Technological Aid Program for Xinjiang(2016AB028)
文摘[Objective] The paper was to understand the occurrence of gray mold of protected tomato and resistance of introduced tomato varieties to gray mold in Shihezi area. [Method] The occurrence status of gray mold in protected tomato in Shawan County, Manas County, the experimental field of Shihezi University and Shihezi Huayu base were investigated during the occurrence period of tomato gray mold, and the resistance of intro-duced variety against tomato gray mold was identified at seedling stage through indoor artificial inoculation method. [Result] The diseased leaf rate of tomato gray mold was 17.52%-100%, the diseased fruit rate was 3.1%-55.69%, and the disease index was 15.98-100. In resistance identifica-tion of different tomato varieties, the disease indexes of Kenfan25, Shifan28, Shifan15, KENFAN-3, KENFAN-5, Shifan35, KENFAN-2 and Shifan-9 were 1.03, 3.1, 1.17, 4.3, 5.32, 5.12, 4.32 and 4.11, respectively. From September to March of the following year, tomato gray mold occurred and was prevalent in greenhouses in Shihezi area. [Conclusion] Shifan28, Kenfan25 and Shifan15 showed resistance to tomato gray mold, while the other five varieties were susceptible to the disease.
基金partially supported by the National Natural Science Foundation of China(Grant Nos.32071905,3217895,and 32201686)Priority Academic Program Development of Jiangsu Higher Education Institutions(Grant No.PAPD-2023-87)+3 种基金Agricultural Equipment Department of Jiangsu University(Grant No.NZXB20210106)National Key Research and Development Program for Young Scientists(Grant No.2022YFD2000013)General Program of Basic Science(Natural Science)Research in Higher Education Institutions of Jiangsu Province(Grant No.23KJB210004)Natural Science Foundation of Jiangsu Province for Youth(Grant No.BK20240880).
文摘This study addresses the challenge posed by the small spore size of tomato gray mold,which hinders its identification and enumeration by conventional techniques.This work presents a novel approach for quantifying spore counts of tomato gray mold using diffraction imaging technology and image processing techniques.To construct a device for acquiring diffraction images of tomato gray mold spores,initially,the hyperspectral data pertaining to the gray mold spores of tomatoes was obtained.The characteristic wavelength of the light source of the diffraction image acquisition device was obtained by smoothing,principal component analysis,and comprehensive coefficient weight calculation.Then,the key parameters of the system were simulated,and the diffraction image acquisition device was built.Finally,tomato gray mold spores were counted based on angular spectrum reconstruction and image processing.The findings indicated that the combined contribution rate of the initial and secondary principal components of the original spectral data obtained from tomato gray mold spore samples amounted to 92.271%.The visible range of 435 nm,475 nm,and 720 nm can be selected as the light source for tomato gray mold’s spore diffraction imaging system.CMOS image sensor was installed 45 mm below the micropore with a diameter of 100μm,and the diffraction image obtained by simulation has a clear diffraction fingerprint.The diffraction imaging system can collect diffraction images of disease spores,and the collected diffraction images have clear diffraction fingerprints.The experimental error range was 5.13%-8.57%,and the average error was 6.42%.The error was within a 95%consistency.Therefore,this study can provide a research basis for the classification and recognition of greenhouse disease spores.
文摘The growth rate method was adopted to measure the inhibitory effect of curcumin,tetrahydrocurcumin,demethoxycurcumin,and bisdemethoxycurcumin on the mycelial growth of Botrytis cinerea and Colletotrichum gloeosporioides.The results showed that the four curcuminoids inhibited the mycelial growth of the two pathogens in a concentration-dependent manner.Bisdemethoxycurcumin at 600 mg/L exerted the strongest inhibitory effect on the mycelial growth of B.cinerea and C.gloeosporioides,with the relative inhibition rates of 98.19%and 100%,respectively;followed by demethoxycurcumin;curcumin exerted the worst inhibitory effect.Toxicity test results also showed that four curcuminoids all had a certain toxicity to B.cinerea and C.gloeosporioides,among which,bisdemethoxycurcumin exhibited the strongest toxicity,with the EC_(50)of 131.125 and 122.235 mg/L,respectively;while curcumin had the lowest toxicity,with the EC_(50)of 273.143 and 194.943 mg/L,respectively.
基金supported by the National Key Research and Development Program(2022YFD1402100,2022YFD1401500)major Basic Research Project of Natural Science Foundation of Shandong Province(ZR2022ZD23)+3 种基金the National Natural Science Foundation(32072500,32272557)Shandong Modern Agricultural Industry Technology System(SDAIT-04-08)Shandong Province Key Research and Development Plan(2022TZXD0025,2021TZXD007-04-4)Taishan Scholar Program of Shandong Province.
文摘Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.
