Objective To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of ...Objective To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia.Methods The chemical synthetic miRNA-展开更多
MicroRNAs（miRNAs） are small,non-coding RNAs that negatively adjust gene expression in multifarious biological processes.However,the regulatory effects of miRNAs on Schwann cells remain poorly understood.Previous mic...MicroRNAs（miRNAs） are small,non-coding RNAs that negatively adjust gene expression in multifarious biological processes.However,the regulatory effects of miRNAs on Schwann cells remain poorly understood.Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction,thereby affecting proliferation and migration of Schwann cells.This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1（Cand1）.Up-regulated expression of miR-148b-3p promoted Schwann cell migration,whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro.Further experiments confirmed that Candl was a direct target of miR-148b-3p,and Candl knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration.These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Candl in vitro.展开更多
Ubiquitin（Ub）is a well-known eukaryotic protein that exerts diverse signaling functions inside the cells,including targeting its substrates for proteasomal degradation（Hershko and Ciechanover,1998）.
Development of effective treatments for neurodegenerative disorders is a clinical conundrum that has puzzled many researchers.Currently available drugs target symptomatic relief rather than suppressing,ceasing or repa...Development of effective treatments for neurodegenerative disorders is a clinical conundrum that has puzzled many researchers.Currently available drugs target symptomatic relief rather than suppressing,ceasing or repairing the devastating neural damages.For Alzheimer’s disease,two classes of procognitive compounds are approved as a treatment.展开更多
Cardiac arrhythmias are among the most common causes of death in the world. Foundational studies established the critical role of ion channel disorders in arrhythmias, yet defects in ion channels themselves, such as m...Cardiac arrhythmias are among the most common causes of death in the world. Foundational studies established the critical role of ion channel disorders in arrhythmias, yet defects in ion channels themselves, such as mutations, may not account for all arrhythmias. Despite the progress made in recent decades, the antiarrhythmic drugs currently available have limited effectiveness,and the majority of these drugs can have proarrhythmic effects. This review describes novel knowledge on cellular mechanisms that cause cardiac arrhythmias, focuses on the dysfunction of subcellular organelles and intracellular logistics, and discusses potential strategies and challenges for developing novel, safe and effective treatments for arrhythmias.展开更多
文摘Objective To investigate the K562 cells biological function and related molecular changes in PTEN-PI3K/AKT signaling pathway of leukemia K562 cells by inhibiting the miRNA-21 expression to explore its pathogenesis of leukemia.Methods The chemical synthetic miRNA-
基金supported by the National Key Basic Research Program of China,No.2014CB542202the National High-Tech R&D Program of China(863 Program),No.2012AA020502+2 种基金the National Natural Science Foundation of China,No.81130080,81371389 and 81571198the Natural Science Foundation of Nantong University of China,No.13040397the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘MicroRNAs（miRNAs） are small,non-coding RNAs that negatively adjust gene expression in multifarious biological processes.However,the regulatory effects of miRNAs on Schwann cells remain poorly understood.Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction,thereby affecting proliferation and migration of Schwann cells.This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1（Cand1）.Up-regulated expression of miR-148b-3p promoted Schwann cell migration,whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro.Further experiments confirmed that Candl was a direct target of miR-148b-3p,and Candl knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration.These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Candl in vitro.
基金supported by a grant from the Korean Health Technology R&D Projects,Ministry of Health&Welfare,Republic of Korea(HI14C2300)to KYR
文摘Ubiquitin（Ub）is a well-known eukaryotic protein that exerts diverse signaling functions inside the cells,including targeting its substrates for proteasomal degradation（Hershko and Ciechanover,1998）.
文摘Development of effective treatments for neurodegenerative disorders is a clinical conundrum that has puzzled many researchers.Currently available drugs target symptomatic relief rather than suppressing,ceasing or repairing the devastating neural damages.For Alzheimer’s disease,two classes of procognitive compounds are approved as a treatment.
基金supported by the National Key Basic Research Program of China (2013CB531100 to Yi-Han Chen)the Major International Joint Research Program of China (81120108004 to Yi-Han Chen)+3 种基金the Key Program of National Natural Science Foundation of China (81530017 to Yi-Han Chen)the National Innovative Research Groups Program of the National Natural Science Foundation of China (81221001 to Yi-Han Chen)the General Program of National Natural Science Foundation of China (81170224, 81270313 to Jun Li, 31271214 to Yi-Han Chen)the National Natural Science Foundation of China (81670295 to Li Lin)
文摘Cardiac arrhythmias are among the most common causes of death in the world. Foundational studies established the critical role of ion channel disorders in arrhythmias, yet defects in ion channels themselves, such as mutations, may not account for all arrhythmias. Despite the progress made in recent decades, the antiarrhythmic drugs currently available have limited effectiveness,and the majority of these drugs can have proarrhythmic effects. This review describes novel knowledge on cellular mechanisms that cause cardiac arrhythmias, focuses on the dysfunction of subcellular organelles and intracellular logistics, and discusses potential strategies and challenges for developing novel, safe and effective treatments for arrhythmias.
