This study investigated the preventive effects of soybean meal peptides(SPs)and their purification peptides(GTYW)on acute alcoholic liver injury.We combined the gut microbiota,metabolites,liver inflammation,and oxidat...This study investigated the preventive effects of soybean meal peptides(SPs)and their purification peptides(GTYW)on acute alcoholic liver injury.We combined the gut microbiota,metabolites,liver inflammation,and oxidative stress indicators to explore the prevention mechanism of SPs and GTYW.Results showed that SPs,GTYW effectively improved the hepatic oxidative stress and inflammatory.Additionally,SPs and GTYW reversed the effects of alcohol on the gut microbiota,which were evident in the increased abundance of Alloprevotella,Parasutterella in the GTYW group and norank_f__Muribaculaceae in the SPs group.Nontargeted metabolomic analysis showed that SPs ameliorated metabolic disorders by regulating phenylalanine,tyrosine and tryptophan biosynthesis,while GTYW regulated metabolites throughα-linolenic acid metabolism and phenylalanine metabolism.Furthermore,significant correlations were observed between gut microbiota,metabolites and liver indicators.These findings confirmed that SPs and GTYW can prevent acute alcoholic liver injury.展开更多
This study investigated the optimal process of solid-state fermentation(SSF)using soybean meal(SBM)as substrate by Aspergillus oryzae and proteolysis of SBM to prepare SBM-derived peptide(SBMP)for chelating with Cu(Ⅱ...This study investigated the optimal process of solid-state fermentation(SSF)using soybean meal(SBM)as substrate by Aspergillus oryzae and proteolysis of SBM to prepare SBM-derived peptide(SBMP)for chelating with Cu(Ⅱ)/Zn(Ⅱ).During the SSF of SBM,the alkaline protease activity of A.oryzae was higher than that of two other fungi,reaching maximum activity(574 U/g)at the fermentation time of 86 h,43%inoculation ratio,and liq-uid-substrate(I/S)ratio of 0.8.The SBMP yield reached a maximum value at 55℃ for 6 h hydrolysis according to the analysis of the degree of hydrolysis(DH)and total Kjeldahl nitrogen(TKN)content in hydrolysates.After ultrafiltration,the SBMP of 1-5 kDa had the best ability to chelate with Cu(Ⅱ)and Zn(Ⅱ).Moreover,the SBMP displayed a higher capacity to chelate with Zn(Ⅱ)(222.7 mg/g)than Cu(Ⅱ)(68.3 mg/g)under optimal condi-tions.Aspartic acid,glutamic acid,lysine,arginine and histidine in SBMP played a critical role at the chelating Cu(Ⅱ)/Zn(Ⅱ).UV and FTIR spectrum analysis revealed that the-COOH and-NH_(2) in SBMP were the main bioactive groups for chelating with Cu(Ⅱ)/Zn(Ⅱ).SEM showed that the surface of SBMP-Cu(Ⅱ)/Zn(Ⅱ)chelate was rough and formed spherical aggregates.These results indicated that combining SSF and proteolysis exhibited great potential for preparing SBMP-metal chelates for developing organometallic supplements.展开更多
基金funded by the National Key R&D Program of China(2022YFD2101002)Jilin Province Science and Technology Youth Talent Support Project(QT202021)Fundamental Research Funds for the Central Universities。
文摘This study investigated the preventive effects of soybean meal peptides(SPs)and their purification peptides(GTYW)on acute alcoholic liver injury.We combined the gut microbiota,metabolites,liver inflammation,and oxidative stress indicators to explore the prevention mechanism of SPs and GTYW.Results showed that SPs,GTYW effectively improved the hepatic oxidative stress and inflammatory.Additionally,SPs and GTYW reversed the effects of alcohol on the gut microbiota,which were evident in the increased abundance of Alloprevotella,Parasutterella in the GTYW group and norank_f__Muribaculaceae in the SPs group.Nontargeted metabolomic analysis showed that SPs ameliorated metabolic disorders by regulating phenylalanine,tyrosine and tryptophan biosynthesis,while GTYW regulated metabolites throughα-linolenic acid metabolism and phenylalanine metabolism.Furthermore,significant correlations were observed between gut microbiota,metabolites and liver indicators.These findings confirmed that SPs and GTYW can prevent acute alcoholic liver injury.
基金supported by the research project of State Key Labo-ratory of Food Science and Technology(Project No.SKLF-ZZB-202122)in Chinathe National Natural Science Foundation of China(22166026,21466022,and 21878139)in ChinaDaily Postdoctoral Support of Jiangxi Province(2020RC08)in China.
文摘This study investigated the optimal process of solid-state fermentation(SSF)using soybean meal(SBM)as substrate by Aspergillus oryzae and proteolysis of SBM to prepare SBM-derived peptide(SBMP)for chelating with Cu(Ⅱ)/Zn(Ⅱ).During the SSF of SBM,the alkaline protease activity of A.oryzae was higher than that of two other fungi,reaching maximum activity(574 U/g)at the fermentation time of 86 h,43%inoculation ratio,and liq-uid-substrate(I/S)ratio of 0.8.The SBMP yield reached a maximum value at 55℃ for 6 h hydrolysis according to the analysis of the degree of hydrolysis(DH)and total Kjeldahl nitrogen(TKN)content in hydrolysates.After ultrafiltration,the SBMP of 1-5 kDa had the best ability to chelate with Cu(Ⅱ)and Zn(Ⅱ).Moreover,the SBMP displayed a higher capacity to chelate with Zn(Ⅱ)(222.7 mg/g)than Cu(Ⅱ)(68.3 mg/g)under optimal condi-tions.Aspartic acid,glutamic acid,lysine,arginine and histidine in SBMP played a critical role at the chelating Cu(Ⅱ)/Zn(Ⅱ).UV and FTIR spectrum analysis revealed that the-COOH and-NH_(2) in SBMP were the main bioactive groups for chelating with Cu(Ⅱ)/Zn(Ⅱ).SEM showed that the surface of SBMP-Cu(Ⅱ)/Zn(Ⅱ)chelate was rough and formed spherical aggregates.These results indicated that combining SSF and proteolysis exhibited great potential for preparing SBMP-metal chelates for developing organometallic supplements.
