目的观察β-catenin/Slug信号特异性抑制剂FH535与EMT的关系,探讨LPCAT1在调节子宫颈癌细胞侵袭、转移和生长中的作用。方法采用sh-NC和sh-LPCAT1转染Hela细胞,利用载体(Vector)组和LPCAT1过表达质粒转染SiHa细胞,将SiHa细胞分为对照组(...目的观察β-catenin/Slug信号特异性抑制剂FH535与EMT的关系,探讨LPCAT1在调节子宫颈癌细胞侵袭、转移和生长中的作用。方法采用sh-NC和sh-LPCAT1转染Hela细胞,利用载体(Vector)组和LPCAT1过表达质粒转染SiHa细胞,将SiHa细胞分为对照组(Con)、LPCAT1组、LPCAT1+FH535组和FH535组。运用CCK-8法和集落形成试验检测子宫颈癌细胞的增殖。通过伤口愈合试验和Transwell实验检测子宫颈癌细胞的转移、侵袭能力。应用Western blot分析细胞中LPCAT1、β-catenin/Slug信号通路和EMT相关蛋白的表达。结果与Vector组相比,LPCAT1组SiHa细胞的活力、集落数、迁移和侵袭细胞数均显著增加(P<0.05);与sh-NC组相比,sh-LPCAT1组Hela细胞的活力、集落数、迁移和侵袭细胞数均显著降低(P<0.05)。与LPCAT1组相比,LPCAT1+FH535组SiHa细胞中Wnt4(1.18±0.05 vs 0.80±0.06)、β-catenin(1.05±0.08 vs 0.77±0.05)、Slug(1.13±0.06 vs 0.28±0.02)、Cyclin D1(0.99±0.06 vs 0.44±0.02)、N-cadherin(0.91±0.07 vs 0.46±0.03)和vimentin(0.95±0.06 vs 0.49±0.03)表达降低(P<0.05),E-cadherin(0.44±0.03 vs 0.58±0.03)表达增加(P<0.05)。此外,与LPCAT1组相比,LPCAT1+FH535组SiHa细胞的集落数(224±15 vs 146±11)、迁移数(85±3 vs 51±4)和侵袭数(166±10 vs 90±5)均降低(P<0.05)。结论LPCAT1表达增加可能通过激活β-catenin/Slug信号通路促进子宫颈癌的转移和进展,LPCAT1的靶向治疗有望提高子宫颈癌患者的预后。展开更多
目的探究E-钙黏蛋白(E-cadherin)、wnt-1诱导分泌蛋白2(wnt-1 inducible secreted protein-2,WISP2)和Slug蛋白与食管胃交界腺癌(adenocarcinoma of the esophagogastric junction,AEG)临床病理因素及预后的关系。方法选取2021年1月至2...目的探究E-钙黏蛋白(E-cadherin)、wnt-1诱导分泌蛋白2(wnt-1 inducible secreted protein-2,WISP2)和Slug蛋白与食管胃交界腺癌(adenocarcinoma of the esophagogastric junction,AEG)临床病理因素及预后的关系。方法选取2021年1月至2022年3月在本院胃肠外科行手术治疗的114例AEG确诊患者,术中留取其癌灶组织与癌旁组织,通过免疫组化染色检测组织中E-cadherin、WISP2、Slug蛋白的表达情况,分析上述指标表达与AEG病理因素的关系,绘制KM曲线探究不同E-cadherin、WISP2、Slug蛋白表达患者的预后情况。结果癌灶组织E-cadherin、WISP2蛋白表达阳性率均低于癌旁组织(P<0.05),但其Slug蛋白表达阳性率高于癌旁组织(P<0.05)。在E-cadherin表达阳性率上,年龄≥50岁患者低于<50岁患者(P<0.05);TNM分期Ⅲ期患者低于Ⅰ~Ⅱ期患者(P<0.05);浸润深度>1/2患者低于≤1/2患者(P<0.05)。在WISP2表达阳性率上,高分化患者低于低、中分化患者(P<0.05);浸润深度>1/2患者低于≤1/2患者(P<0.05)。在Slug蛋白表达阳性率上,高分化高于低、中分化患者(P<0.05);浸润深度>1/2患者高于≤1/2患者(P<0.05);淋巴结转移患者高于无淋巴结转移患者(P<0.05)。Slug蛋白均分别与E-cadherin、WISP2呈负相关(r=-0.301、-0.289,P<0.05)。E-cadherin表达阳性组、阴性组生存时间分别为为(19.71±0.84)个月、(15.85±1.02)个月,阳性组生存时间长于阴性组(P<0.05)。WISP2表达阳性组、阴性组生存时间分别为(19.84±0.83)个月、(18.52±1.82)个月,阳性组生存时间长于阴性组(P<0.05)。Slug表达阳性组、阴性组生存时间分别为为(16.06±0.93)个月、(19.66±0.92)个月,阳性组生存时间短于阴性组(P<0.05)。结论E-cadherin、WISP2、Slug蛋白在AEG患者中异常表达,其表达与AEG的发生、转移以及侵袭等病理因素相关,可作为临床预测AEG患者预后的潜在指标。展开更多
The liquid slug length distribution is crucial for designing the downstream processing system with mul-tiphase pipeline. Experiments were conducted in a 133m long horizontal test loop. The measurements were per-formed...The liquid slug length distribution is crucial for designing the downstream processing system with mul-tiphase pipeline. Experiments were conducted in a 133m long horizontal test loop. The measurements were per-formed by conductivity probes to determine the liquid slug length distribution. The data covered both the slug and plug flow regimes. From experimental results, the mean liquid slug lengths were relatively insensitive to gas and liquid flow rates in the higher mixture velocity range. But in the lower mixture velocity range, the mean liquid slug length decreased and then increased with mixture velocity. It shows that the development length of slug flow was longer than x/D=1157. A slug tracking model was adapted to study the evolution of liquid slug length distribution in a horizontal pipeline. In the present model, the wake effect of elongated bubble and the pressure drop due to accel-eration are taken into account and random slug lengths are introduced at the entrance. The results of the model are compared with the measured slug length distributions of slug flow regime. It shows that the predicted mean and maximum slug lengths are in agreement with the experimental data at x/D=1157 and the form of the slug length distributions is also predicted well by the model.展开更多
Slug initiation and subsequem evolution along a 5.0 cm ID, 16m long horizomal pipe are experimentally studied. The transient characteristics of interfacial structures are described by using simultaneous measuremeres o...Slug initiation and subsequem evolution along a 5.0 cm ID, 16m long horizomal pipe are experimentally studied. The transient characteristics of interfacial structures are described by using simultaneous measuremeres of the liquid height at multiple locations along the pipe. Various effects of superficial gas and liquid velocities and pressure oscillation on the slug initiation and evolution along the pipe are illustrated. It is found that the slug is initiated by a deterministic orocess with reolenishmem and deoletion of liquid near the inlet for the superficial gas velocity USG〈3.0m·s^-1 and by a stochastic process with wave coalescence along the pipe for USG〉3.0m·s^-1.The evolution of the slugs is strongly attected by superhclal gas and liquid veloclties for USG〈3.0m·s^-1 but weakly affected by the superficial gas velocity for USG〉3.0 m·s . The suppression of pressure oscillation at the pipe inlet significantly delays the onset of slugging, with slugs forming postponed further downstream. The slug frequency at the outlet is, however, not affected by the variation in the pressure oscillation.展开更多
目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-...目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-钙黏蛋白和波形蛋白的表达情况变化,另外使用穿透小室法检测细胞的侵袭能力在转染前后的变化情况。结果:转染pc DNA 3.1-Slug和miR-140到Eca-109中后细胞变得细长。转染pc DNA 3.1-Slug的Eca-109中E-钙黏素表达下调,N-钙黏蛋白和波形蛋白表达上调,细胞穿透基质胶的数量明显增多。转染miR-140的Eca-109中E-钙黏素表达上调,N-钙黏蛋白和波形蛋白表达下调,细胞穿透基质胶的数量明显减少。在食管癌细胞中miR-140和Slug的表达呈负相关(r=-0.96)。结论:转录因子Slug能够提高食管癌上皮细胞的间质转化能力和侵袭能力,而miR-140是通过调控Slug来调节食管癌细胞侵袭性的。展开更多
目的研究ROCK/SLUG信号通路在内皮素-1(endo-thelin-1,ET-1)促进人卵巢癌细胞上皮向间充质转分化(epi-thelial to mesenchymal transition,EMT)中的作用。方法 ET-1处理人卵巢癌细胞株SK-OV-3和CaOV3,或共用ROCK的活化突变体转染细胞或...目的研究ROCK/SLUG信号通路在内皮素-1(endo-thelin-1,ET-1)促进人卵巢癌细胞上皮向间充质转分化(epi-thelial to mesenchymal transition,EMT)中的作用。方法 ET-1处理人卵巢癌细胞株SK-OV-3和CaOV3,或共用ROCK的活化突变体转染细胞或加入ROCK的抑制剂Y27632,并转染含SLUG启动子的pGL3质粒与Renilla质粒。实验末用Boyden小室体外侵袭实验检测细胞侵袭能力,细胞免疫荧光染色观察细胞形态,报告基因检测试剂盒检测SLUG启动子活性,用实时定量PCR和Western bot方法检测EMT相关基因的表达。结果 ET-1诱导SK-OV-3和CaOV3发生与EMT相一致的形态和基因变化,促进其细胞侵袭力;ET-1与内皮素A受体(endothelin A receptor,ETAR)结合,促进转录因子SLUG的转录;ET-1促进ROCK及fibronectin的表达,同时转染ROCK的活化突变体,促进ET-1诱导的fibronectin表达以及细胞侵袭力的增加。相反,ROCK抑制剂Y27632抑制ET-1对fibronectin表达以及细胞侵袭力的促进作用;转染ROCK的活化突变体,上调SLUG基因转录启动子活性促进其转录,抑制E-cadherin的转录。相反,ROCK的抑制剂Y27632抑制SLUG基因启动子的活性。结论 ET-1通过活化ROCK/SLUG通路促进人卵巢癌细胞发生EMT。展开更多
文摘目的观察β-catenin/Slug信号特异性抑制剂FH535与EMT的关系,探讨LPCAT1在调节子宫颈癌细胞侵袭、转移和生长中的作用。方法采用sh-NC和sh-LPCAT1转染Hela细胞,利用载体(Vector)组和LPCAT1过表达质粒转染SiHa细胞,将SiHa细胞分为对照组(Con)、LPCAT1组、LPCAT1+FH535组和FH535组。运用CCK-8法和集落形成试验检测子宫颈癌细胞的增殖。通过伤口愈合试验和Transwell实验检测子宫颈癌细胞的转移、侵袭能力。应用Western blot分析细胞中LPCAT1、β-catenin/Slug信号通路和EMT相关蛋白的表达。结果与Vector组相比,LPCAT1组SiHa细胞的活力、集落数、迁移和侵袭细胞数均显著增加(P<0.05);与sh-NC组相比,sh-LPCAT1组Hela细胞的活力、集落数、迁移和侵袭细胞数均显著降低(P<0.05)。与LPCAT1组相比,LPCAT1+FH535组SiHa细胞中Wnt4(1.18±0.05 vs 0.80±0.06)、β-catenin(1.05±0.08 vs 0.77±0.05)、Slug(1.13±0.06 vs 0.28±0.02)、Cyclin D1(0.99±0.06 vs 0.44±0.02)、N-cadherin(0.91±0.07 vs 0.46±0.03)和vimentin(0.95±0.06 vs 0.49±0.03)表达降低(P<0.05),E-cadherin(0.44±0.03 vs 0.58±0.03)表达增加(P<0.05)。此外,与LPCAT1组相比,LPCAT1+FH535组SiHa细胞的集落数(224±15 vs 146±11)、迁移数(85±3 vs 51±4)和侵袭数(166±10 vs 90±5)均降低(P<0.05)。结论LPCAT1表达增加可能通过激活β-catenin/Slug信号通路促进子宫颈癌的转移和进展,LPCAT1的靶向治疗有望提高子宫颈癌患者的预后。
文摘目的探究E-钙黏蛋白(E-cadherin)、wnt-1诱导分泌蛋白2(wnt-1 inducible secreted protein-2,WISP2)和Slug蛋白与食管胃交界腺癌(adenocarcinoma of the esophagogastric junction,AEG)临床病理因素及预后的关系。方法选取2021年1月至2022年3月在本院胃肠外科行手术治疗的114例AEG确诊患者,术中留取其癌灶组织与癌旁组织,通过免疫组化染色检测组织中E-cadherin、WISP2、Slug蛋白的表达情况,分析上述指标表达与AEG病理因素的关系,绘制KM曲线探究不同E-cadherin、WISP2、Slug蛋白表达患者的预后情况。结果癌灶组织E-cadherin、WISP2蛋白表达阳性率均低于癌旁组织(P<0.05),但其Slug蛋白表达阳性率高于癌旁组织(P<0.05)。在E-cadherin表达阳性率上,年龄≥50岁患者低于<50岁患者(P<0.05);TNM分期Ⅲ期患者低于Ⅰ~Ⅱ期患者(P<0.05);浸润深度>1/2患者低于≤1/2患者(P<0.05)。在WISP2表达阳性率上,高分化患者低于低、中分化患者(P<0.05);浸润深度>1/2患者低于≤1/2患者(P<0.05)。在Slug蛋白表达阳性率上,高分化高于低、中分化患者(P<0.05);浸润深度>1/2患者高于≤1/2患者(P<0.05);淋巴结转移患者高于无淋巴结转移患者(P<0.05)。Slug蛋白均分别与E-cadherin、WISP2呈负相关(r=-0.301、-0.289,P<0.05)。E-cadherin表达阳性组、阴性组生存时间分别为为(19.71±0.84)个月、(15.85±1.02)个月,阳性组生存时间长于阴性组(P<0.05)。WISP2表达阳性组、阴性组生存时间分别为(19.84±0.83)个月、(18.52±1.82)个月,阳性组生存时间长于阴性组(P<0.05)。Slug表达阳性组、阴性组生存时间分别为为(16.06±0.93)个月、(19.66±0.92)个月,阳性组生存时间短于阴性组(P<0.05)。结论E-cadherin、WISP2、Slug蛋白在AEG患者中异常表达,其表达与AEG的发生、转移以及侵袭等病理因素相关,可作为临床预测AEG患者预后的潜在指标。
基金Supported by the National Natural Science Foundation of China (No.50536020 and 50323001).
文摘The liquid slug length distribution is crucial for designing the downstream processing system with mul-tiphase pipeline. Experiments were conducted in a 133m long horizontal test loop. The measurements were per-formed by conductivity probes to determine the liquid slug length distribution. The data covered both the slug and plug flow regimes. From experimental results, the mean liquid slug lengths were relatively insensitive to gas and liquid flow rates in the higher mixture velocity range. But in the lower mixture velocity range, the mean liquid slug length decreased and then increased with mixture velocity. It shows that the development length of slug flow was longer than x/D=1157. A slug tracking model was adapted to study the evolution of liquid slug length distribution in a horizontal pipeline. In the present model, the wake effect of elongated bubble and the pressure drop due to accel-eration are taken into account and random slug lengths are introduced at the entrance. The results of the model are compared with the measured slug length distributions of slug flow regime. It shows that the predicted mean and maximum slug lengths are in agreement with the experimental data at x/D=1157 and the form of the slug length distributions is also predicted well by the model.
基金Supported by the National Natural Science Foundation of China （50521604） and the State Key Development Program for Basic Research of China （2007CB029804）.
文摘Slug initiation and subsequem evolution along a 5.0 cm ID, 16m long horizomal pipe are experimentally studied. The transient characteristics of interfacial structures are described by using simultaneous measuremeres of the liquid height at multiple locations along the pipe. Various effects of superficial gas and liquid velocities and pressure oscillation on the slug initiation and evolution along the pipe are illustrated. It is found that the slug is initiated by a deterministic orocess with reolenishmem and deoletion of liquid near the inlet for the superficial gas velocity USG〈3.0m·s^-1 and by a stochastic process with wave coalescence along the pipe for USG〉3.0m·s^-1.The evolution of the slugs is strongly attected by superhclal gas and liquid veloclties for USG〈3.0m·s^-1 but weakly affected by the superficial gas velocity for USG〉3.0 m·s . The suppression of pressure oscillation at the pipe inlet significantly delays the onset of slugging, with slugs forming postponed further downstream. The slug frequency at the outlet is, however, not affected by the variation in the pressure oscillation.
文摘目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-钙黏蛋白和波形蛋白的表达情况变化,另外使用穿透小室法检测细胞的侵袭能力在转染前后的变化情况。结果:转染pc DNA 3.1-Slug和miR-140到Eca-109中后细胞变得细长。转染pc DNA 3.1-Slug的Eca-109中E-钙黏素表达下调,N-钙黏蛋白和波形蛋白表达上调,细胞穿透基质胶的数量明显增多。转染miR-140的Eca-109中E-钙黏素表达上调,N-钙黏蛋白和波形蛋白表达下调,细胞穿透基质胶的数量明显减少。在食管癌细胞中miR-140和Slug的表达呈负相关(r=-0.96)。结论:转录因子Slug能够提高食管癌上皮细胞的间质转化能力和侵袭能力,而miR-140是通过调控Slug来调节食管癌细胞侵袭性的。
文摘目的研究ROCK/SLUG信号通路在内皮素-1(endo-thelin-1,ET-1)促进人卵巢癌细胞上皮向间充质转分化(epi-thelial to mesenchymal transition,EMT)中的作用。方法 ET-1处理人卵巢癌细胞株SK-OV-3和CaOV3,或共用ROCK的活化突变体转染细胞或加入ROCK的抑制剂Y27632,并转染含SLUG启动子的pGL3质粒与Renilla质粒。实验末用Boyden小室体外侵袭实验检测细胞侵袭能力,细胞免疫荧光染色观察细胞形态,报告基因检测试剂盒检测SLUG启动子活性,用实时定量PCR和Western bot方法检测EMT相关基因的表达。结果 ET-1诱导SK-OV-3和CaOV3发生与EMT相一致的形态和基因变化,促进其细胞侵袭力;ET-1与内皮素A受体(endothelin A receptor,ETAR)结合,促进转录因子SLUG的转录;ET-1促进ROCK及fibronectin的表达,同时转染ROCK的活化突变体,促进ET-1诱导的fibronectin表达以及细胞侵袭力的增加。相反,ROCK抑制剂Y27632抑制ET-1对fibronectin表达以及细胞侵袭力的促进作用;转染ROCK的活化突变体,上调SLUG基因转录启动子活性促进其转录,抑制E-cadherin的转录。相反,ROCK的抑制剂Y27632抑制SLUG基因启动子的活性。结论 ET-1通过活化ROCK/SLUG通路促进人卵巢癌细胞发生EMT。
