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生物信息学和分子对接分析HuNoVs非结构蛋白NS3
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作者 王道 肖亚梅 刘丹 《微生物学杂志》 北大核心 2025年第1期85-95,共11页
为了深入探究人类诺如病毒(Human noroviruses,HuNoVs)的NS3非结构蛋白的结构特征和小分子药物,运用多种生物信息学方法ProtParam、ProtScale、SignalP 5.0、TMHMM 2.0、NCBI Conserved Domains、SOPMA、SWISS-MODEL、YinOYang 1.2、Net... 为了深入探究人类诺如病毒(Human noroviruses,HuNoVs)的NS3非结构蛋白的结构特征和小分子药物,运用多种生物信息学方法ProtParam、ProtScale、SignalP 5.0、TMHMM 2.0、NCBI Conserved Domains、SOPMA、SWISS-MODEL、YinOYang 1.2、NetPhos 3.1、ABCpred、SYFPEITHI、Prankweb和Discovery Studio等预测NS3蛋白的同源性、理化性质、翻译后修饰位点、二级/三级结构、B/T细胞抗原表位、活性口袋位点及抑制化合物。HuNoVs的NS3蛋白是由363个氨基酸组成的稳定亲水性蛋白,相对分子量为39748.79 Da,理论等电点为6.37;NS3蛋白与其他病毒解旋酶序列相似性为32.52%,无信号肽和跨膜结构,二级结构以α-螺旋和无规则卷曲为主,具有2个功能结构域,38个O-糖基化位点和21个磷酸化位点,7个B-细胞抗原表位和19个T-细胞抗原表位;还存在6个活性口袋位置和5个潜在的小分子抑制化合物。本文为阐明人类诺如病毒非结构蛋白NS3的结构和在病毒性肠胃炎中的作用机制提供了理论依据,也为研发和筛选新疫苗和药物奠定了基础。 展开更多
关键词 人类诺如病毒 非结构蛋白ns3 生物信息学 分子对接
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Integrated high-performance and accurate shaping technology of low-cost powder metallurgy titanium alloys: A comprehensive review 被引量:1
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作者 Xuemeng Gan Shaofu Li +1 位作者 Shunyuan Xiao Yafeng Yang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第3期413-426,共14页
The practical engineering applications of powder metallurgy (PM) Ti alloys produced through cold compaction and pressure-less sintering are impeded by poor sintering densification, embrittlement caused by excessive O ... The practical engineering applications of powder metallurgy (PM) Ti alloys produced through cold compaction and pressure-less sintering are impeded by poor sintering densification, embrittlement caused by excessive O impurities, and severe sintering deforma-tion resulting from the use of heterogeneous powder mixtures. This review presents a summary of our previous work on addressing the above challenges. Initially, we proposed a novel strategy using reaction-induced liquid phases to enhance sintering densification. Near- complete density (relative density exceeding 99%) was achieved by applying the above strategy and newly developed sintering aids. By focusing on the O-induced embrittlement issue, we determined the onset dissolution temperature of oxide films in the Ti matrix. On the basis of this finding, we established a design criterion for effective O scavengers that require reaction with oxide films before their dissol-ution. Consequently, a ductile PM Ti alloy was successfully obtained by introducing 0.3wt% NdB6 as the O scavenger. Lastly, a powder- coating strategy was adopted to address the sintering deformation issue. The ultrafine size and shell-like distribution characteristics of coating particles ensured rapid dissolution and homogeneity in the Ti matrix, thereby facilitating linear shrinkage during sintering. As a result, geometrically complex Ti alloy parts with high dimensional accuracy were fabricated by using the coated powder. Our fundament-al findings and related technical achievements enabled the development of an integrated production technology for the high-performance and accurate shaping of low-cost PM Ti alloys. Additionally, the primary engineering applications and progress in the industrialization practice of our developed technology are introduced in this review. 展开更多
关键词 powder metallurgy titanium sintering densification oxygen scavenging accurate shaping
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猪细小病毒NS1基因编码蛋白的原核表达及多克隆抗体的制备
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作者 帅文娜 郭子强 +8 位作者 李佳乐 罗梦 李丽薇 周艳君 姜一峰 童武 童光志 李兆龙 高飞 《中国兽医科学》 北大核心 2025年第3期314-320,共7页
本研究旨在制备PPV-NS1的多克隆抗体,并用猪细小病毒(PPV)和真核质粒pcDNA3.1(+)-NS1-3×Flag进行双重验证。首先将PPV的非结构蛋白编码基因NS1克隆至原核表达载体pETDuet,构建原核质粒pET-PPV-NS1。接着,将原核质粒pET-PPV-NS1转... 本研究旨在制备PPV-NS1的多克隆抗体,并用猪细小病毒(PPV)和真核质粒pcDNA3.1(+)-NS1-3×Flag进行双重验证。首先将PPV的非结构蛋白编码基因NS1克隆至原核表达载体pETDuet,构建原核质粒pET-PPV-NS1。接着,将原核质粒pET-PPV-NS1转化至感受态E.coli BL21细胞中,经由1 mmol/LIPTG诱导以后,通过SDS-PAGE和Western-blot对NS1表达进行检测,结果表明,NS1基因在感受态细胞中得到了良好的表达。其次,将纯化后获得的NS1蛋白采用多点注射的方式对大白兔进行免疫,制备获得针对重组NS1蛋白的多克隆抗体。然后,将真核质粒pcDNA3.1(+)-NS1-3×Flag转染HEK 293T细胞;同时PPV感染ST细胞后收取蛋白样品,用上述制备的NS1多克隆抗体进行验证。结果显示,制备的NS1多克隆抗体具有良好的反应性和特异性。 展开更多
关键词 猪细小病毒 ns1蛋白 多克隆抗体 原核表达
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ROE格式的物理增强图神经网络求解Euler与层流不可压NS方程
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作者 宋尚校 姜龙祥 +2 位作者 王丽媛 褚新坤 张浩 《应用数学和力学》 北大核心 2025年第1期55-71,共17页
近年来,融合物理信息的深度学习方法为偏微分方程的求解提供了一个新的思路.然而,到目前为止,大多数工作在解空间存在间断的问题上的计算精度不高,时间外推能力差.针对以上两个问题,该文提出了使用图神经网络结合流体计算领域的ROE格式... 近年来,融合物理信息的深度学习方法为偏微分方程的求解提供了一个新的思路.然而,到目前为止,大多数工作在解空间存在间断的问题上的计算精度不高,时间外推能力差.针对以上两个问题,该文提出了使用图神经网络结合流体计算领域的ROE格式融合方程或数据信息的模型———ROE-PIGNN.数值实验表明,该模型在求解由Euler方程控制的激波管问题时,可达到与传统ROE格式相当的计算精度,并具备一定时间范围的外推能力.最后,对由Navier-Stokes(NS)方程控制的二维圆柱绕流问题进行了求解,实验结果表明:模型可以预测后续的周期性流动,并实现对部分关键位置流动结构的更精确的复现,相比纯数据驱动,误差降低了60%. 展开更多
关键词 深度学习 图神经网络 ROE格式 EULER方程 ns方程
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猫杯状病毒NS2蛋白的截短表达及多克隆抗体的制备与应用
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作者 董宁宁 张琦 +4 位作者 谈晓梅 李娜 朱雯琪 刘光清 孟春春 《畜牧与兽医》 北大核心 2025年第2期78-83,共6页
旨在鉴定并深入研究NS2蛋白在猫杯状病毒(FCV)感染中的作用。根据FCV SH2014株的基因序列,利用生物信息学技术对NS2蛋白进行了分析,将其在110 aa处截短为2个基因片段(NS2-N和NS2-C),将2个NS2-N相连接。筛选出成功表达的重组蛋白,使用弗... 旨在鉴定并深入研究NS2蛋白在猫杯状病毒(FCV)感染中的作用。根据FCV SH2014株的基因序列,利用生物信息学技术对NS2蛋白进行了分析,将其在110 aa处截短为2个基因片段(NS2-N和NS2-C),将2个NS2-N相连接。筛选出成功表达的重组蛋白,使用弗氏佐剂乳化后免疫新西兰大白兔。通过Western blot和间接免疫荧光试验(IFA)验证多克隆抗体的特异性识别能力。结果:成功构建了pET-32a-NS2-N和pET-32a-NS2-N-N重组质粒,并在上清液和包涵体中稳定表达了预期大小的重组蛋白。制备的多克隆抗体能特异性识别多个病毒株的NS2蛋白。结论:成功制备兔抗NS2蛋白的多克隆抗体,为深入研究FCV NS2蛋白的结构和功能提供了试验材料,为进一步阐明该病毒的致病机制奠定基础。 展开更多
关键词 猫杯状病毒 ns2蛋白截短 原核表达 多克隆抗体
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C、N含量对NS1402耐蚀合金晶间腐蚀性能的影响
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作者 舒玮 谷宇 《金属热处理》 北大核心 2025年第4期85-88,共4页
研究了不同C、N含量对NS1402合金晶间腐蚀性能的影响。结果表明,NS1402合金试样经675℃和750℃敏化处理后的微观组织中无析出物产生。675℃敏化条件下,当N含量小于0.01%时,降低C含量对合金晶间腐蚀速率的影响较小。敏化温度升高至750℃... 研究了不同C、N含量对NS1402合金晶间腐蚀性能的影响。结果表明,NS1402合金试样经675℃和750℃敏化处理后的微观组织中无析出物产生。675℃敏化条件下,当N含量小于0.01%时,降低C含量对合金晶间腐蚀速率的影响较小。敏化温度升高至750℃后,C、N含量均在0.01%以上的晶间腐蚀试样表现出较为严重的腐蚀特征,平均腐蚀率为0.03 mm/月,腐蚀深度达24μm。C、N含量降低至0.01%以下后,合金在750℃敏化条件下表现出优良的耐晶间腐蚀性能,平均腐蚀率只有0.01 mm/月。 展开更多
关键词 ns1402耐蚀合金 C含量 N含量 晶间腐蚀
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Joint Optimization of Precoding and Constellation Shaping for Faster-than-Nyquist Signaling
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作者 Kang Weimin 《China Communications》 SCIE CSCD 2024年第11期141-149,共9页
Faster-than-Nyquist(FTN)signaling is a potential scheme for the sixth generation(6G)communication system to improve the spectral efficiency(SE).In this paper,we propose a joint optimization algorithm of precoding and ... Faster-than-Nyquist(FTN)signaling is a potential scheme for the sixth generation(6G)communication system to improve the spectral efficiency(SE).In this paper,we propose a joint optimization algorithm of precoding and constellation shaping for FTN signaling,which is based on simulated optimization via the bare bones particle swarm optimization(BBPSO).The information-theoretical analysis and simulated error performance show that the proposed method is efficient,which can get a significant improvement in terms of average mutual information(AMI)and bit error rate(BER)performance.The simulated BER results verify the theoretical AMI analysis.Compared with the conventional regular 16QAM FTN scheme,when BER is at 10-5,the joint optimized scheme can obtain 0.50 dB and 0.60 dB performance gain with SE at 3.077 bits/s/Hz and 3.282 bits/s/Hz,respectively.Therefore,the proposed scheme is reliable,and thus suitable for the 6G communication. 展开更多
关键词 constellation shaping faster-than-Nyquist PRECODING spectral efficiency
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Asia-Pacific Regional Economic Cooperation Under the Reshaping of Global Production and Supply Chains
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作者 Liu Chenyang 《Contemporary World》 2024年第3期20-25,共6页
As part of the important trends of the current development of the world economy,the reshaping of global industry and supply chains interacts with the intensified changes unprecedented in the past century in an increas... As part of the important trends of the current development of the world economy,the reshaping of global industry and supply chains interacts with the intensified changes unprecedented in the past century in an increasingly close manner.“Benefit-sharing and risk-sharing”,the characteristics of global industry and supply chains,have a profound influence on the distribution of benefits among countries and the effectiveness of global economic governance. 展开更多
关键词 CHAIns intensified shaping
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A study on the treatment of chloasma with moxibustion based on the theory of“Yang transforming Qi,and Yin shaping”
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作者 Mi Yang Hong-Yi Lan +2 位作者 Hong Wu Xu Wang Xin-Ju Li 《Clinical Research Communications》 2024年第3期23-28,共6页
Background:“Yang Transforming Qi and Yin shaping”comes from“Plain Question Yin and Yang should be like the theory”.Chinese medicine believes that the imbalance of yin and yang is the cause of human diseases.One of... Background:“Yang Transforming Qi and Yin shaping”comes from“Plain Question Yin and Yang should be like the theory”.Chinese medicine believes that the imbalance of yin and yang is the cause of human diseases.One of the strengths of TCM in treating diseases is holistic regulation.The theory of“Yang transforming Qi,and Yin shaping”implies a strong holistic view.Chloasma,one of the common skin diseases,seriously affects the physical and mental health of patients due to its unclear etiology,difficult treatment and easy recurrence.The occurrence and development of chloasma are closely related to internal organs.This paper is based on the theory of internal meridian,“Yang transforming Qi,and Yin shaping”which attaches importance to the concept of“Yang Qi”,from a new understanding of the pathogenesis etiology of chloasma.Methods:The author believes that the incidence of the disease is the deficiency of Yang Qi,which can’t promote the movement of Qi and blood to nourish the face,resulting in tangible material condensation,so proposes that this disease needs to be promoted by Yang transforming Qi and warm to dissipate.Moxibustion belongs to the fire,and its warming effect can dissipate the tangible evil.Results:Combined with clinical practice and the theory of internal meridian,the author elaborated that moxibustion is one of the effective methods for treating chloasma,which provides a new way of thinking for the treatment of chloasma.Conclusion:“Yang Transforming Qi and Yin shaping”can be used to guide the treatment of chloasma. 展开更多
关键词 Yang Transforming Qi and Yin shaping MOXIBUSTION CHLOASMA
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Optical transmittance and pulse shape discrimination of polystyrene/poly(methyl methacrylate)-based plastic scintillators
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作者 Yi-Yao Liang Ying-Du Liu +2 位作者 Pu-Sen Wang Jie Bao Xiao-Ping Ouyang 《Nuclear Science and Techniques》 2025年第1期94-102,共9页
Polymethyl methacrylate(PMMA)is an optically transparent thermoplastic with favorable processing conditions.In this study,a series of plastic scintillators are prepared via thermal polymerization,and the impact of PMM... Polymethyl methacrylate(PMMA)is an optically transparent thermoplastic with favorable processing conditions.In this study,a series of plastic scintillators are prepared via thermal polymerization,and the impact of PMMA content on their transparency and pulse shape discrimination(PSD)ability is investigated.The fabricated samples,comprising a polystyrene(PS)-PMMA matrix,30.0 wt%2,5-diphenyloxazole(PPO),and 0.2 wt%9,10-diphenylanthracene(DPA),exhibit high transparency with transmissivity ranging from 70.0 to 90.0%(above 415.0 nm)and demonstrate excellent n/γdiscrimination capability.Transparency increased with increasing PMMA content across the entire visible light spectrum.However,the PSD performance gradually deteriorated when the aromatic matrix was replaced with PMMA.The scintillator containing 20.0 wt%PMMA demonstrated the best stability concerning PSD properties and relative light yields. 展开更多
关键词 Neutron detection Plastic scintillation Pulse shape discrimination Polymethyl-methacrylate POLYSTYRENE
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Shape Memory Polymers with Self-folding Deformation and Multi-stimulus Response
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作者 Lan Zhang Wei Zhang +2 位作者 Qiushi Wang Suqian Ma Xia Yan 《Journal of Bionic Engineering》 2025年第1期238-250,共13页
Shape Memory Polymers(SMPs)need to be given a temporary shape in advance to realize the shape memory process,but the manual shaping process is cumbersome and has low precision.Here,we propose a universal applicable me... Shape Memory Polymers(SMPs)need to be given a temporary shape in advance to realize the shape memory process,but the manual shaping process is cumbersome and has low precision.Here,we propose a universal applicable method for 4D printing self-folding SMPs by pre-stretching extruded filaments during 3D printing,the temporary shape of the SMPs were designed and fixed during 3D printing.Prepared samples can automatically perform shape memory process under stimulation without manual temporary shape programming process.Furthermore,using carbon ink as a photothermal conversion agent enables the 4D printing SMPs to have thermal and light response characteristics.In addition,some bionic applications of self-folding SMPs were demonstrated,such as self-morphing grasper,DNA double helix structures,programmable sequential switching mimosa,self-folding box and human hand.The combination of SMP and 3D printing fully takes advantage of 4D printing technology,and the self-folding SMPs show great potential applications in the fields of tissue engineering scaffold,self-folding robots,self-assembly system and so on. 展开更多
关键词 3D printing 4D printing Shape memory Multi-stimulus response Self-folding
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Perspectives on additive manufacturing for warhead applications
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作者 Hao Xue Qiang Zhou +1 位作者 Chuan Xiao Guangyan Huang 《Defence Technology(防务技术)》 2025年第1期225-251,共27页
According to different damage modes,warheads are roughly divided into three types:fragmentation warheads,shaped charge warheads,and penetrating warheads.Due to limitations in material and structural manufacturing,trad... According to different damage modes,warheads are roughly divided into three types:fragmentation warheads,shaped charge warheads,and penetrating warheads.Due to limitations in material and structural manufacturing,traditional manufacturing methods make it difficult to fully utilize the damage ability of the warhead.Additive manufacturing(AM)technology can fabricate complex structures,with classified materials composition and customized components,while achieving low cost,high accuracy,and rapid production of the parts.The maturity of AM technology has brought about a new round of revolution in the field of warheads.In this paper,we first review the principles,classifications,and characteristics of different AM technologies.The development trends of AM technologies are pointed out,including multi-material AM technology,hybrid AM technology,and smart AM technology.From our survey,PBF,DED,and EBM technologies are mainly used to manufacture warhead damage elements.FDM and DIW technologies are mainly used to manufacture warhead charges.Then,the research on the application of AM technology in three types of warhead and warhead charges was reviewed and the existing problems and progress of AM technologies in each warhead were analyzed.Finally,we summarized the typical applications and look forward to the application prospects of AM technology in the field of warheads. 展开更多
关键词 Additive manufacturing Fragmentation warhead Shaped charge warhead Penetrating warhead Warhead charge
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Tailoring temperature response for a multimode fiber
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作者 Han Gao Haifeng Hu Qiwen Zhan 《Opto-Electronic Science》 2025年第1期12-24,共13页
This work introduces special states for light in multimode fibers featuring strongly enhanced or reduced correlations be-tween output fields in the presence of environmental temperature fluctuations.Using experimental... This work introduces special states for light in multimode fibers featuring strongly enhanced or reduced correlations be-tween output fields in the presence of environmental temperature fluctuations.Using experimentally measured multi-tem-perature transmission matrix,a set of temperature principal modes that exhibit resilience to disturbances caused by tem-perature fluctuations can be generated.Reversing this concept also allows the construction of temperature anti-principal modes,with output profiles more susceptible to temperature influences than the unmodulated wavefront.Despite changes in the length of the multimode fiber within the temperature-fluctuating region,the proposed approach remains capable of robustly controlling the temperature response within the fiber.To illustrate the practicality of the proposed spe-cial state,a learning-empowered fiber specklegram temperature sensor based on temperature anti-principal mode sensi-tization is proposed.This sensor exhibits outstanding superiority over traditional approaches in terms of resolution and accuracy.These novel states are anticipated to have wide-ranging applications in fiber communication,sensing,imaging,and spectroscopy,and serve as a source of inspiration for the discovery of other novel states. 展开更多
关键词 multimode fiber principal mode wavefront shaping optical fiber sensor temperature response
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Theoretical and Experimental Analysis of Nonlinear Large Tensile Deformation of Superelastic SMA-Based Honeycomb Structures
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作者 Yahao Wang Wenjiong Chen +1 位作者 Renjing Gao Shutian Liu 《Acta Mechanica Solida Sinica》 2025年第1期45-64,共20页
Honeycomb structures of shape memory alloy(SMA)have become one of the most promising materials for flexible skins of morphing aircraft due to their excellent mechanical properties.However,due to the nonlinear material... Honeycomb structures of shape memory alloy(SMA)have become one of the most promising materials for flexible skins of morphing aircraft due to their excellent mechanical properties.However,due to the nonlinear material and geometric large deformation,the SMA honeycomb exhibits significant and complex nonlinearity in the skin and there is a lack of relevant previous research.In this paper,the nonlinear properties of the SMA honeycomb structure with arbitrary geometry are investigated for the first time for large deformation flexible skin applications by theoretical and experimental analysis.Firstly,a novel theoretical model of SMA honeycomb structure considering both material and geometric nonlinearity is proposed,and the corresponding calculation method of nonlinear governing equations is given based upon the shooting method and Runge–Kutta method.Then,the tensile behaviors of four kinds of SMA honeycomb structures,i.e.,U-type,V-type,cosine-type,and trapezoid-type,are analyzed and predicted by the proposed theoretical model and compared with the finite element analysis(FEA)results.Moreover,the tensile experiments were carried out by stretching U-type and V-type honeycomb structures to a global strain of 60%and 40%,respectively,to perform large deformation analysis and verify the theoretical model.Finally,experimental verification and finite element validation show that the curves of the theoretical model results,experimental results,and simulation results are in good agreement,illustrating the generalizability and accuracy of the proposed theoretical model.The theoretical model and experimental investigations in this paper are considered to provide an effective foundation for analyzing and predicting the mechanical behavior of SMA honeycomb flexible skins with large extensional deformations. 展开更多
关键词 Morphing wing Flexible skin Large deformation Geometric nonlinearity Material nonlinearity Shape memory alloy
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Comprehensive study of pulse shape discrimination in a Ga-doped zinc oxide scintillating detector
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作者 Kuo Zhao Liang Chen +5 位作者 Ning Lv Lei-Dang Zhou Shi-Yi He Jin-Lu Ruan Han Wang Xiao-Ping Ouyang 《Nuclear Science and Techniques》 2025年第3期38-49,共12页
Doping with Ga effectively enhances the crystal quality and optical detection efficiency of zinc oxide(Zn O)single crystals,which has attracted considerable research interest in radiation detection.The application of ... Doping with Ga effectively enhances the crystal quality and optical detection efficiency of zinc oxide(Zn O)single crystals,which has attracted considerable research interest in radiation detection.The application of Zn O:Ga(GZO)in nuclear energy is particularly significant and fascinating at the fundamental level,enabling neutron/gamma discrimination while preserving the response time properties of the single crystal in sub-nanoseconds,maximizing the effective counting rate of the pulsed radiation field.In this study,the single-particle waveform discrimination characteristics of GZO were evaluated for five charged particles(α,β,H^(+),Li^(+),and O^(8+)and two prevalent uncharged particles(neutrons and gamma rays).Based on the timecorrelation single-photon counting(TCSPC)method,the luminescence decay time constants of the charged particles in the GZO crystal were determined as follows:1.21 ns for H^(+),1.50 ns for Li^(+),1.70 ns for O^(8+),1.56 ns forαparticles,and 1.09 ns forβparticles.Visible differences in the excitation time spectra curves were observed.Using the conventional time-domain or frequency-domain waveform discrimination techniques,waveform discrimination of 14.9 Me V neutrons and secondary gamma rays generated by the CPNG-6 device based on GZO scintillation was successfully implemented.The neutron signal constituted 77.93%of the total,indicating that GZO exhibited superior neutron/gamma discrimination sensitivity compared with that of a commercial stilbene crystal.Using the neutron/gamma screening outcomes,we reconstructed the voltage pulse height,charge height,and neutron multiplication time spectra of the pulsed neutron radiation field.The reconstructed neutron multiplication time spectrum exhibited a deviation of less than 3%relative to the result obtained using a commercial stilbene scintillator.This is the first report in the open literature on the neutron/gamma discrimination and reconstruction of Zn O pulsed radiation-field information. 展开更多
关键词 Zinc oxide Scintillation crystal Pulse shape discrimination Radiation luminescence Pulsed neutron radiation field
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Chords of Friendship An Indonesian musician’s insights into music education in China and Indonesia and his vision for the future of classical music as a cultural bridge
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作者 Mntony Hardi 《China Report ASEAN》 2025年第4期38-39,共2页
More than a simple concert pianist,Wilson Chu is an Indonesian musical diplomacy force.As a distinguished performer,composer,and educator,Wilson has left an indelible mark on the international stage by seamlessly blen... More than a simple concert pianist,Wilson Chu is an Indonesian musical diplomacy force.As a distinguished performer,composer,and educator,Wilson has left an indelible mark on the international stage by seamlessly blending Western classical music with Southeast Asian tradition.Since 2019,he has been the youngest Associate Professor of Piano at the College of Chinese and ASEAN Arts(CCAA)at Chengdu University,where he has dedicated himself to shaping the next generation of musicians. 展开更多
关键词 Indonesian musician music education friendship chords classical music Indonesia shaping next generation musicians
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BVDV NS5A蛋白在原核系统中的优化表达与纯化鉴定
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作者 高明阳 杨宣叶 +3 位作者 吴玉湖 王进千 胡欣妍 周建华 《中国奶牛》 2025年第1期35-41,共7页
本研究构建了牛病毒性腹泻病毒NS5A蛋白重组质粒并在原核表达系统中表达,但分析过程中发现其主要形式为包涵体,因此通过更换条件使BVDV NS5A蛋白可溶,同时可纯化出蛋白,为后面结构功能分析、抗体制备以及酶联免疫吸附实验奠定基础。以... 本研究构建了牛病毒性腹泻病毒NS5A蛋白重组质粒并在原核表达系统中表达,但分析过程中发现其主要形式为包涵体,因此通过更换条件使BVDV NS5A蛋白可溶,同时可纯化出蛋白,为后面结构功能分析、抗体制备以及酶联免疫吸附实验奠定基础。以实验室保存的毒株为试验材料,采用逆转录PCR(reversetranscription PCR,RT-PCR)技术扩增NS5A基因,构建pET-28a-NS5A和pET-32a-NS5A表达质粒,转化于大肠杆菌BL21(DE3)和Rosetta gami 2(DE3)感受态细胞中,使用IPTG进行诱导表达,对表达产物进行SDS-PAGE和Western-blot分析,最后根据表达条件的摸索,纯化出可溶性的NS5A蛋白。成功克隆出了BVDV NS5A基因,序列全长为1 488bp,编码497个氨基酸,理论分子质量为55.8ku。成功构建了原核表达质粒pET-28a-NS5A和pET-32a-NS5A,其在大肠杆菌BL21(DE3)和Rosetta gami 2(DE3)可表达以包涵体形式为主的NS5A重组蛋白,分子质量分别约为61ku和75ku,经过体表达条件的摸索,pET-32a-NS5A质粒在大肠杆菌BL21(DE3)中,经过25℃低温诱导出现较多可溶性,并纯化出NS5A蛋白。本研究成功克隆出了1 488bp的NS5A基因,经不同条件的诱导表达后,得到可溶性NS5A重组蛋白。 展开更多
关键词 牛病毒性腹泻病毒 ns5A蛋白 原核表达 鉴定 可溶性
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Ultra-high-Q photonic crystal nanobeam cavity for etchless lithium niobate on insulator(LNOI)platform
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作者 Zhi Jiang Cizhe Fang +8 位作者 Xu Ran Yu Gao Ruiqing Wang Jianguo Wang Danyang Yao Xuetao Gan Yan Liu Yue Hao Genquan Han 《Opto-Electronic Advances》 2025年第1期59-70,共12页
The expansive spectral coverage and superior optical properties of lithium niobate(LN)offer a comprehensive suite of tools for exploring novel functionalities.Achieving high-quality(Q)photonic resonator cavities is cr... The expansive spectral coverage and superior optical properties of lithium niobate(LN)offer a comprehensive suite of tools for exploring novel functionalities.Achieving high-quality(Q)photonic resonator cavities is crucial for enhancing light-matter interactions.However,this task is challenging as the device performance is heavily dependent on the fabrication quality of the LN.In this paper,we present experimental validation of an etchless approach to fabricating high-Q photonic crystal nanobeam cavities(PCNBCs).We successfully fabricate PCNBCs with Q factors exceeding 105 while maintaining high transmittance by capitalizing on the low waveguide loss and high fabrication tolerance of TE-polarized mode.Remarkably,the Q factor achieved here exceeds previous reports on etchless LN PCNBCs by over an order of magnitude.Benefiting from this advancement,we further explore a variety of optical functions,including thermo-optic tuning,optically induced bistability,and Fano line shapes generation.These findings present promising prospects for a versatile platform technique,facilitating the development of high-performance electro-optic or acousto-optic modulators,optical logic devices,and quantum photonics,highlighting its significant impact in the field of photonic integration. 展开更多
关键词 photonic crystal nanobeam cavity LNOI platform high Q thermo-optic tuning BISTABILITY Fano line shapes
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Hollow fiber-based strain sensors with desirable modulus and sensitivity at effective deformation for dexterous electroelastomer cylindrical actuator
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作者 Yang Zhang Keqi Deng +10 位作者 Tingting Shen Yong Huang Zhenjin Xu Jinhui Zhang Hang Jin Xin Liu Lida Xu Lianjie Lu Shiying Li Daoheng Sun Dezhi Wu 《Microsystems & Nanoengineering》 2025年第1期375-388,共14页
The electroelastomer cylindrical actuators,a typical representation of soft actuators,have recently aroused increasing interest owing to their advantages in flexibility,deformability,and spatial utilization rate.Propr... The electroelastomer cylindrical actuators,a typical representation of soft actuators,have recently aroused increasing interest owing to their advantages in flexibility,deformability,and spatial utilization rate.Proprioception is crucial for controlling and monitoring the shape and position of these actuators.However,most existing flexible sensors have a modulus mismatch with the actuation unit,hindering the free movement of these actuators.Herein,a low-modulus strain sensor based on laser-induced cellular graphitic flakes(CGF)onto the surface of hollow TPU fibers(HTF)is present.Through the electrostatic self-assembly technology,the flexible sensor features a unique hybrid sensing unit including soft HTF as substrate and rigid CGF as conductive path.As a result,the sensor simultaneously possesses desirable modulus(~0.155 MPa),a gauge factor of 220.3(25%<ε<50%),fast response/recovery behaviors(31/62 ms),and a low detection limit(0.1%strain).Integrating the sensor onto the electroelastomer cylindrical actuators enables precise measurement of deformation modes,directions,and quantity.As proof-of-concept demonstrations,a prototype soft robot with high-precision perception is successfully designed,achieving real-time detection of its deformations during the crawling process.Thus,the proposed scheme sheds new light on the development of intelligent soft robots. 展开更多
关键词 dexterous actuator PROPRIOCEPTION electroelastomer cylindrical actuatorsa electroelastomer soft actuatorshave controlling monitoring shape position hollow fiber modulus mismatch
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不同生物型牛病毒性腹泻病毒NS4B蛋白的生物信息学分析
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作者 吴树康 梁滋旺 +3 位作者 姜伟 金彩虹 陈岩 郭明佳 《中国动物检疫》 2025年第2期108-113,共6页
牛病毒性腹泻病毒(BVDV)是一种引起牛病毒性腹泻的高度致死性病原,根据生物型不同可分为致细胞病变型(CP)及非致细胞病变型(NCP)2种。本研究通过多种生物信息学方法,对这2种生物型BVDV毒株NS4B蛋白的差异及相似性进行了比较。结果显示:... 牛病毒性腹泻病毒(BVDV)是一种引起牛病毒性腹泻的高度致死性病原,根据生物型不同可分为致细胞病变型(CP)及非致细胞病变型(NCP)2种。本研究通过多种生物信息学方法,对这2种生物型BVDV毒株NS4B蛋白的差异及相似性进行了比较。结果显示:2种生物型BVDV的NS4B蛋白均属于不含信号肽和跨膜区的不稳定性疏水蛋白,其N端具有较高变异性;亚细胞定位预测发现,NS4B蛋白不含有线粒体导肽(mTP)或分泌通道信号肽(SP),且定位在内质网和线粒体中的概率最高;高级结构预测发现,2种生物型NS4B蛋白的二级与三级结构相似,均以α-螺旋为主;B细胞抗原表位分析发现,NCP型BVDV含有8个B细胞抗原表位肽段,CP型含有9个;蛋白翻译后修饰预测发现,NCP型BVDV具有1个特异性N-糖基化修饰位点,NCP型BVDV具有36个潜在的磷酸化修饰位点,而CP型具有35个。结果表明:2种生物型BVDV NS4B蛋白的基本理化性质及蛋白质结构相似性较高,但亚细胞定位情况和蛋白翻译后修饰有所不同,推测N端氨基酸序列的高突变性及蛋白翻译后修饰的不同可能会影响不同生物型BVDV与发病动物临床症状之间的关系。 展开更多
关键词 牛病毒性腹泻病毒 ns4B蛋白 生物信息学 遗传变异
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