AIM:To directly quantify peroxynitrite(ONOO-)using a highly sensitive fluorescence resonance energy transfer probe RN-NA,investigate the association between ONOOand primary open angle glaucoma(POAG),and clarify whethe...AIM:To directly quantify peroxynitrite(ONOO-)using a highly sensitive fluorescence resonance energy transfer probe RN-NA,investigate the association between ONOOand primary open angle glaucoma(POAG),and clarify whether RN-NA could be used as a potential tool for POAG diagnosis.METHODS:Plasma and aqueous humor(AH)samples were collected from POAG patients(n=100,age:59.70±6.87y)and age-related cataract(ARC)patients(n=100,age:61.15±4.60y)admitted to our hospital.Next,RN-NA was used to detect ONOO-in plasma and AH samples,and the relationship between ONOO-level and POAG was analyzed using binary logistic regression.Besides,Pearson correlation analysis was applied to characterize the correlation of the levels of ONOO-with the patients’age,intraocular pressure(IOP),and mean deviation of visual field testing.The ONOO-scavenger MnTMPyP was employed to treat the 3-morpholinosyndnomine(SIN-1)-induced ocular hypertension in mice(n=7,6-8wk).Finally,the IOP and ONOO-in both eyes were measured 30min after the last drug treatment.RESULTS:ONOO-levels of AH and plasma were significantly higher in the POAG group than in the ARC group(P<0.01).Additionally,ONOO-levels were closely correlated with POAG in a binary logistic regression analysis[odds ratio(OR)=1.008,95%confidence interval(CI):1.002-1.013,P<0.01 for AH;OR=1.004,95%CI:1.002-1.006,P<0.001 for plasma].Pearson correlation analysis showed that ONOO-levels in AH or plasma were positively associated with visual field defects(R=0.51,P<0.01 for AH;R=0.45,P<0.001 for plasma),and ONOO-levels in plasma and AH were correlated in the POAG group(R=0.69,P<0.001).However,administering MnTMPyP to mouse eyes reversed the elevated IOP caused by SIN-1(P<0.05).CONCLUSION:ONOO-levels in AH and plasma,detected by RN-NA,are significantly related to POAG and positively correlated with visual field defects in POAG patients.Hence,ONOO-is a potential biomarker of POAG,especially advanced POAG.Besides,anti-nitration compounds may be novel ocular hypotensive agents based on the animal study.展开更多
A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition...A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of Co doped g-C_(3)N_(4)can catalyze the reaction of O-phenylenediamine and H_(2)O_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 nm.Through the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by HPLC.The result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.展开更多
Direct measurement of dipole potential in biological membranes has been impossible and 3-hydroxyflavones(3HFs) have allowed detection of changes in dipole potential in biological systems.In the present study,sixteen d...Direct measurement of dipole potential in biological membranes has been impossible and 3-hydroxyflavones(3HFs) have allowed detection of changes in dipole potential in biological systems.In the present study,sixteen derivatives of 3HF with aliphatic hydrocarbon chains of different lengths at 4′-position and 6-position were synthesized.The basic fluorescence properties of 3HFs are maintained in all the probes in terms of strong blue shift in maximum fluorescence emission wavelength and>100 fold increase in quantum yield in organic solvents and in dioleoylphosphatidylcholine(DOPC) small unilamellar vesicles(SUV) in comparison to in aqueous Hepes buffer(15 mmol/L,pH 7.4).More importantly,the ability of the new compounds to report dipole potential changes in biological systems are also maintained,since all the new probes showed spectrum properties that are similar to yet different from that of F4N1,which potentially may allow more sensitive measurement of the dipole potential change in membranes.展开更多
Landing gear is one of the important components of Chang'E-3 Probe.The device was used to absorb the impact energy of Chang'E-3 Probe during the landing process.After landing on the moon,it can support the lan...Landing gear is one of the important components of Chang'E-3 Probe.The device was used to absorb the impact energy of Chang'E-3 Probe during the landing process.After landing on the moon,it can support the lander steadily for a long time.In order to assure the reliability of the landing gear and deal with the extremely severe landing conditions,many reliability measures were adopted in the design procedure,such as selection of cushion materials,optimization of mechanism configuration,design of deployment mode,allocation of buffer force and control of its variation.The successful landing on the moon of Chang'E-3 Probe has completely verified the high reliability of the landing gear.展开更多
Fluorescence properties of 1-pheny1-3-(4'-nitrophenyl) pyrazoline (PNP) were studied inbulk polymerization process of methylmethacrylate (MMA). The fluorescence intensity of PNPwas enhanced and the emission maximu...Fluorescence properties of 1-pheny1-3-(4'-nitrophenyl) pyrazoline (PNP) were studied inbulk polymerization process of methylmethacrylate (MMA). The fluorescence intensity of PNPwas enhanced and the emission maximum was blue shifted with the polymerization progress. Inthe period of auto-acceleration of the polymerization the enhancement of fluorescence intensityand blue shift of peak wavelength in spectra could be observed evidently. This means that thesolvatochromic properties of PNP are influenced not only by the solvent polarity but also by theviscosity of the medium (especially by the phase transitiott). In solid state PNP emits from thecharge transfer excited state without solvent relaxation. The transient emission spectra and theresults from Bakhshiev model of solvent relaxation coincide with that from the polymerizationexperiment.展开更多
Europium (Eu￣(3+)) doped polycrystalline (LaO)_3BO_3 has been synthesized by solid state reaction with Li_2CO_3 as flux at 1200℃ for 10 h in air. X- ray powder diffraction shows that ( LaO)_3BO_3 crystallizes in a...Europium (Eu￣(3+)) doped polycrystalline (LaO)_3BO_3 has been synthesized by solid state reaction with Li_2CO_3 as flux at 1200℃ for 10 h in air. X- ray powder diffraction shows that ( LaO)_3BO_3 crystallizes in a monoclinic cell [a= 0. 69214 (9)nm , b=1. 2929(3) nm , c=1. 4590 (3 ) nm . β= 99. 35 (2)°,V= 1. 2882(4 ) nm￣3 and Z= 8] with a possible space group of P2_1/m or P2_1. The predominance of electric-dipole transition ￣5D_0→ ￣7F_2 in the fluorescence spectrum , as well as the splitting of the￣7F_j ground states of Eu￣(3+) reveals that the lanthanum atoms distribute in the sites with C_1 or C_s symmetry in this structure.展开更多
A 3D probe used for NC\|copying process is proposed in the paper. The construction of parallel springs is adopted in the probe. It has compact structure and little volume. By adjusting spring force and improving sensi...A 3D probe used for NC\|copying process is proposed in the paper. The construction of parallel springs is adopted in the probe. It has compact structure and little volume. By adjusting spring force and improving sensitivity, the probe can be used in CMM. Errors of the probe are analysed. Performances of the probe are verified by test of measuring force, verification of precision of single axis and plane precision.展开更多
A pH-sensor with a pKa value of 5.05 based on 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene（BODIPY） dye was synthesized and demonstrated to be highly sensitive within a pH range of 4.0--6.0 and was highly photostable, ...A pH-sensor with a pKa value of 5.05 based on 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene（BODIPY） dye was synthesized and demonstrated to be highly sensitive within a pH range of 4.0--6.0 and was highly photostable, which makes it a potential pH fluorescent probe. The new pH-sensor will find its application in studying acidic organelles in living cells.展开更多
We report a case of hepatocellular carcinoma (HCC) with chronic hepatitis C virus (HCV) infection, suc-cessfully treated with percutaneous radiofrequency ablation (RFA) under new Live trhree-dimensional (3D) echo guid...We report a case of hepatocellular carcinoma (HCC) with chronic hepatitis C virus (HCV) infection, suc-cessfully treated with percutaneous radiofrequency ablation (RFA) under new Live trhree-dimensional (3D) echo guidance for hearts probe before [1], but ,at the time, perform RFA with a new Live3D probe de- veloped flants. A 61 year-old Japanese man had a 3.5 cm in diameter HCC nodule in the liver S3 region. We applied the Live 3D ultrasonography during RFA therapy with a cool-tip needle electrode. The echo guidance facilitated an accurate approach for the needle puncture. The guidance was also useful for confirming, whether an adequate safety marging for the nodule had been obtained. Thus new Live 3D ul-trasonography echo technique appears to provide safe guidance of RFA needles via accurate targeting of HCC nodules, thereby allowing real-time visualize- tion when combined with echo contrast.展开更多
开发地球电子辐射带的数据同化模型,对于理解辐射带电子的动态演化过程和辐射带空间天气预报具有重要意义.结合范阿伦卫星的辐射带电子观测数据和外辐射带三维扩散模型,采用卡尔曼滤波算法,本文开发了基于Fortran语言的外辐射带电子三...开发地球电子辐射带的数据同化模型,对于理解辐射带电子的动态演化过程和辐射带空间天气预报具有重要意义.结合范阿伦卫星的辐射带电子观测数据和外辐射带三维扩散模型,采用卡尔曼滤波算法,本文开发了基于Fortran语言的外辐射带电子三维数据同化模型(Three-dimensional Data Assimilative Model of Outer Radiation belt Electrons,简称TDAMORE),实现对L^(*)=3~7、能量范围为0.1~5 MeV、投掷角范围为5°~90°的外辐射带电子时空变化过程的三维重构.通过对2018年8月期间外辐射带电子通量演化过程的重构,证实TDAMORE模型可以较好地重现不同能量和不同投掷角电子通量在磁暴前后的演化特征.通过分析电子通量的观测和同化结果之间的相关系数、平均误差、平均绝对误差和均方误差,发现对于能量低于4 MeV的电子,观测与同化结果之间的相关系数基本大于0.8且误差相对较低.而对于更高能量的电子,观测与同化结果之间的误差相对较高,这可能是同化模型忽略了电磁离子回旋波对电子的散射损失导致的.展开更多
基金Supported by the National Natural Science Foundation of China(No.81870692,No.82070959,No.82271082)the Shanghai Committee of Science and Technology,China(No.20S31905800)Clinical Research Plan of SHDC(No.SHDC2020CR6029).
文摘AIM:To directly quantify peroxynitrite(ONOO-)using a highly sensitive fluorescence resonance energy transfer probe RN-NA,investigate the association between ONOOand primary open angle glaucoma(POAG),and clarify whether RN-NA could be used as a potential tool for POAG diagnosis.METHODS:Plasma and aqueous humor(AH)samples were collected from POAG patients(n=100,age:59.70±6.87y)and age-related cataract(ARC)patients(n=100,age:61.15±4.60y)admitted to our hospital.Next,RN-NA was used to detect ONOO-in plasma and AH samples,and the relationship between ONOO-level and POAG was analyzed using binary logistic regression.Besides,Pearson correlation analysis was applied to characterize the correlation of the levels of ONOO-with the patients’age,intraocular pressure(IOP),and mean deviation of visual field testing.The ONOO-scavenger MnTMPyP was employed to treat the 3-morpholinosyndnomine(SIN-1)-induced ocular hypertension in mice(n=7,6-8wk).Finally,the IOP and ONOO-in both eyes were measured 30min after the last drug treatment.RESULTS:ONOO-levels of AH and plasma were significantly higher in the POAG group than in the ARC group(P<0.01).Additionally,ONOO-levels were closely correlated with POAG in a binary logistic regression analysis[odds ratio(OR)=1.008,95%confidence interval(CI):1.002-1.013,P<0.01 for AH;OR=1.004,95%CI:1.002-1.006,P<0.001 for plasma].Pearson correlation analysis showed that ONOO-levels in AH or plasma were positively associated with visual field defects(R=0.51,P<0.01 for AH;R=0.45,P<0.001 for plasma),and ONOO-levels in plasma and AH were correlated in the POAG group(R=0.69,P<0.001).However,administering MnTMPyP to mouse eyes reversed the elevated IOP caused by SIN-1(P<0.05).CONCLUSION:ONOO-levels in AH and plasma,detected by RN-NA,are significantly related to POAG and positively correlated with visual field defects in POAG patients.Hence,ONOO-is a potential biomarker of POAG,especially advanced POAG.Besides,anti-nitration compounds may be novel ocular hypotensive agents based on the animal study.
基金supported by the National Natural Science Foundation of China(21804050)the National Key R and D Program of China(2018YFD0901003)+2 种基金the Science and Technology Planning Project of Xiamen,China(3502Z20183031)the Fujian Provincial Fund Project(2018J01432)the Xiamen Science and Technology Planning Project,China(3502Z20183031)。
文摘A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of Co doped g-C_(3)N_(4)can catalyze the reaction of O-phenylenediamine and H_(2)O_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 nm.Through the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by HPLC.The result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.
文摘Direct measurement of dipole potential in biological membranes has been impossible and 3-hydroxyflavones(3HFs) have allowed detection of changes in dipole potential in biological systems.In the present study,sixteen derivatives of 3HF with aliphatic hydrocarbon chains of different lengths at 4′-position and 6-position were synthesized.The basic fluorescence properties of 3HFs are maintained in all the probes in terms of strong blue shift in maximum fluorescence emission wavelength and>100 fold increase in quantum yield in organic solvents and in dioleoylphosphatidylcholine(DOPC) small unilamellar vesicles(SUV) in comparison to in aqueous Hepes buffer(15 mmol/L,pH 7.4).More importantly,the ability of the new compounds to report dipole potential changes in biological systems are also maintained,since all the new probes showed spectrum properties that are similar to yet different from that of F4N1,which potentially may allow more sensitive measurement of the dipole potential change in membranes.
基金National Science and Technology Major Project,China
文摘Landing gear is one of the important components of Chang'E-3 Probe.The device was used to absorb the impact energy of Chang'E-3 Probe during the landing process.After landing on the moon,it can support the lander steadily for a long time.In order to assure the reliability of the landing gear and deal with the extremely severe landing conditions,many reliability measures were adopted in the design procedure,such as selection of cushion materials,optimization of mechanism configuration,design of deployment mode,allocation of buffer force and control of its variation.The successful landing on the moon of Chang'E-3 Probe has completely verified the high reliability of the landing gear.
基金This project was supported by the National Natural Science Foundation of China
文摘Fluorescence properties of 1-pheny1-3-(4'-nitrophenyl) pyrazoline (PNP) were studied inbulk polymerization process of methylmethacrylate (MMA). The fluorescence intensity of PNPwas enhanced and the emission maximum was blue shifted with the polymerization progress. Inthe period of auto-acceleration of the polymerization the enhancement of fluorescence intensityand blue shift of peak wavelength in spectra could be observed evidently. This means that thesolvatochromic properties of PNP are influenced not only by the solvent polarity but also by theviscosity of the medium (especially by the phase transitiott). In solid state PNP emits from thecharge transfer excited state without solvent relaxation. The transient emission spectra and theresults from Bakhshiev model of solvent relaxation coincide with that from the polymerizationexperiment.
文摘Europium (Eu￣(3+)) doped polycrystalline (LaO)_3BO_3 has been synthesized by solid state reaction with Li_2CO_3 as flux at 1200℃ for 10 h in air. X- ray powder diffraction shows that ( LaO)_3BO_3 crystallizes in a monoclinic cell [a= 0. 69214 (9)nm , b=1. 2929(3) nm , c=1. 4590 (3 ) nm . β= 99. 35 (2)°,V= 1. 2882(4 ) nm￣3 and Z= 8] with a possible space group of P2_1/m or P2_1. The predominance of electric-dipole transition ￣5D_0→ ￣7F_2 in the fluorescence spectrum , as well as the splitting of the￣7F_j ground states of Eu￣(3+) reveals that the lanthanum atoms distribute in the sites with C_1 or C_s symmetry in this structure.
基金Supported by the National Natural Science Foundation of China (50175037)
文摘A 3D probe used for NC\|copying process is proposed in the paper. The construction of parallel springs is adopted in the probe. It has compact structure and little volume. By adjusting spring force and improving sensitivity, the probe can be used in CMM. Errors of the probe are analysed. Performances of the probe are verified by test of measuring force, verification of precision of single axis and plane precision.
基金Supported by the State Basic Research Project of China(No.2002CB713808)
文摘A pH-sensor with a pKa value of 5.05 based on 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene（BODIPY） dye was synthesized and demonstrated to be highly sensitive within a pH range of 4.0--6.0 and was highly photostable, which makes it a potential pH fluorescent probe. The new pH-sensor will find its application in studying acidic organelles in living cells.
文摘We report a case of hepatocellular carcinoma (HCC) with chronic hepatitis C virus (HCV) infection, suc-cessfully treated with percutaneous radiofrequency ablation (RFA) under new Live trhree-dimensional (3D) echo guidance for hearts probe before [1], but ,at the time, perform RFA with a new Live3D probe de- veloped flants. A 61 year-old Japanese man had a 3.5 cm in diameter HCC nodule in the liver S3 region. We applied the Live 3D ultrasonography during RFA therapy with a cool-tip needle electrode. The echo guidance facilitated an accurate approach for the needle puncture. The guidance was also useful for confirming, whether an adequate safety marging for the nodule had been obtained. Thus new Live 3D ul-trasonography echo technique appears to provide safe guidance of RFA needles via accurate targeting of HCC nodules, thereby allowing real-time visualize- tion when combined with echo contrast.
文摘开发地球电子辐射带的数据同化模型,对于理解辐射带电子的动态演化过程和辐射带空间天气预报具有重要意义.结合范阿伦卫星的辐射带电子观测数据和外辐射带三维扩散模型,采用卡尔曼滤波算法,本文开发了基于Fortran语言的外辐射带电子三维数据同化模型(Three-dimensional Data Assimilative Model of Outer Radiation belt Electrons,简称TDAMORE),实现对L^(*)=3~7、能量范围为0.1~5 MeV、投掷角范围为5°~90°的外辐射带电子时空变化过程的三维重构.通过对2018年8月期间外辐射带电子通量演化过程的重构,证实TDAMORE模型可以较好地重现不同能量和不同投掷角电子通量在磁暴前后的演化特征.通过分析电子通量的观测和同化结果之间的相关系数、平均误差、平均绝对误差和均方误差,发现对于能量低于4 MeV的电子,观测与同化结果之间的相关系数基本大于0.8且误差相对较低.而对于更高能量的电子,观测与同化结果之间的误差相对较高,这可能是同化模型忽略了电磁离子回旋波对电子的散射损失导致的.
