L-arginine serves as a substrate for the production of nitric oxide(NO)in animals,and it can also impact muscle fiber characteristics and meat quality in these animals.The present study was designed to explore the eff...L-arginine serves as a substrate for the production of nitric oxide(NO)in animals,and it can also impact muscle fiber characteristics and meat quality in these animals.The present study was designed to explore the effects of adding 1%L-arginine to a basal diet regimen on the muscle fiber characteristics and meat quality of Mongolian sheep.Dietary L-arginine supplementation reduced shear force in the longissimus thoracis(LT)and increased a*in biceps femoris(BF)muscles(p<0.05).L-arginine supplementation also increased the proportion of type IIA muscle fiber in the LT(p<0.05)and type I muscle fiber in the BF(p<0.05)while reducing both the diameter and CSA of type IIB muscle fiber in both the LT and BF(CSA in LT,p<0.01;all others,p<0.05).L-arginine treatment was also associated with the upregulation of MyHC IIa(LT),MyHC I(BF),nNOS(LT&BF),AMPKα1(BF),PGC-1α(LT&BF)(PGC-1αin BF,p<0.01;all others,p<0.05),together with an increase in nNOS content(LT,p<0.01;BF,p<0.05).Dietary L-arginine supplementation was associated with a significant increase in the post-slaughter tenderness of lamb meat,which is related to transitions in muscle fiber types.The gene expression and nNOS analysis results generated herein further indicate that this effect is mediated by the NO/AMPK/PGC-1αpathway.Further studies are thus warranted to provide further insight into the role that NO signaling plays in controlling the associations between L-arginine,muscle fiber characteristics,and meat quality.展开更多
The growth performance and histochemical characteristics of breast muscle fibers were used to estimate the standardized ileal digestible(SID)Lys requirements for 10-to 21-d-old male broilers.Three hundred and sixty 10...The growth performance and histochemical characteristics of breast muscle fibers were used to estimate the standardized ileal digestible(SID)Lys requirements for 10-to 21-d-old male broilers.Three hundred and sixty 10-d-old Ross 308 broilers(290±16.6 g)were allocated to 6 diets in a randomized complete block design with 6 replicate cages per treatment and 10 birds per cage.The 6 experimental diets were formulated to contain equally spaced increasing levels of SID Lys from 0.86%to 1.36%.The data were analyzed using the MIXED procedure of SAS.The Lys requirements were estimated by the NLIN procedure of SAS.An increase in dietary SID Lys from 0.86%to 1.36%resulted in a quadratic increase(P<0.05)in body weight gain(BWG),gain to feed ratio(G:F),breast weight,muscle cross-sectional area(MCSA),and fiber area.The SID Lys requirements based on the one-slope broken-line,quadratic line,the first intercept between the plateau of the one-slope broken-line and quadratic-line models and 95%of the upper asymptote of the quadratic-line model were estimated to be 1.01%,1.19%,1.08%,and 1.13%for BWG,1.06%,1.22%,1.11%,and 1.16%for G:F,1.10%,1.29%,1.19%,and 1.22%for breast weight,1.06%,1.22%,1.12%,and 1.16%for MCSA,and 1.14%,1.22%,1.16%,and 1.16%for breast muscle fiber area,respectively.It was concluded that the SID Lys requirements for broilers at the age of 10 to 21 d depended on the response variables used for estimation,and that histochemical characteristics of breast muscle fibers could be good indicators for estimating SID Lys requirements.展开更多
基金This research was supported by the National Natural Science Foundation of China(32060519)Natural Science Foundation of Inner Mongolia(2021MS03010)+2 种基金National Natural Science Foundation of China(32160589)Natural Science Foundation of Inner Mongolia(2020ZD11)Scientific and Technological Innovation Guidance Award Fund Project of Inner Mongolia.
文摘L-arginine serves as a substrate for the production of nitric oxide(NO)in animals,and it can also impact muscle fiber characteristics and meat quality in these animals.The present study was designed to explore the effects of adding 1%L-arginine to a basal diet regimen on the muscle fiber characteristics and meat quality of Mongolian sheep.Dietary L-arginine supplementation reduced shear force in the longissimus thoracis(LT)and increased a*in biceps femoris(BF)muscles(p<0.05).L-arginine supplementation also increased the proportion of type IIA muscle fiber in the LT(p<0.05)and type I muscle fiber in the BF(p<0.05)while reducing both the diameter and CSA of type IIB muscle fiber in both the LT and BF(CSA in LT,p<0.01;all others,p<0.05).L-arginine treatment was also associated with the upregulation of MyHC IIa(LT),MyHC I(BF),nNOS(LT&BF),AMPKα1(BF),PGC-1α(LT&BF)(PGC-1αin BF,p<0.01;all others,p<0.05),together with an increase in nNOS content(LT,p<0.01;BF,p<0.05).Dietary L-arginine supplementation was associated with a significant increase in the post-slaughter tenderness of lamb meat,which is related to transitions in muscle fiber types.The gene expression and nNOS analysis results generated herein further indicate that this effect is mediated by the NO/AMPK/PGC-1αpathway.Further studies are thus warranted to provide further insight into the role that NO signaling plays in controlling the associations between L-arginine,muscle fiber characteristics,and meat quality.
基金This work was carried out with the support of the “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ013571)” Rural Development Administration, Republic of Korea.
文摘The growth performance and histochemical characteristics of breast muscle fibers were used to estimate the standardized ileal digestible(SID)Lys requirements for 10-to 21-d-old male broilers.Three hundred and sixty 10-d-old Ross 308 broilers(290±16.6 g)were allocated to 6 diets in a randomized complete block design with 6 replicate cages per treatment and 10 birds per cage.The 6 experimental diets were formulated to contain equally spaced increasing levels of SID Lys from 0.86%to 1.36%.The data were analyzed using the MIXED procedure of SAS.The Lys requirements were estimated by the NLIN procedure of SAS.An increase in dietary SID Lys from 0.86%to 1.36%resulted in a quadratic increase(P<0.05)in body weight gain(BWG),gain to feed ratio(G:F),breast weight,muscle cross-sectional area(MCSA),and fiber area.The SID Lys requirements based on the one-slope broken-line,quadratic line,the first intercept between the plateau of the one-slope broken-line and quadratic-line models and 95%of the upper asymptote of the quadratic-line model were estimated to be 1.01%,1.19%,1.08%,and 1.13%for BWG,1.06%,1.22%,1.11%,and 1.16%for G:F,1.10%,1.29%,1.19%,and 1.22%for breast weight,1.06%,1.22%,1.12%,and 1.16%for MCSA,and 1.14%,1.22%,1.16%,and 1.16%for breast muscle fiber area,respectively.It was concluded that the SID Lys requirements for broilers at the age of 10 to 21 d depended on the response variables used for estimation,and that histochemical characteristics of breast muscle fibers could be good indicators for estimating SID Lys requirements.
