This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic analyses.The results show that ...This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic analyses.The results show that the Co-bearing steel exhibits finer blocks and a lower ductile-brittle transition temperature than the steel without Co.Moreover,the Co-bearing steel reveals higher transformation rates at the intermediate stage with bainite volume fraction ranging from around 0.1 to 0.6.The improved impact toughness of the Co-bearing steel results from the higher dense block boundaries dominated by the V1/V2 variant pair.Furthermore,the addition of Co induces a larger transformation driving force and a lower bainite start temperature(BS),thereby contributing to the refinement of blocks and the increase of the V1/V2 variant pair.These findings would be instructive for the composition,microstructure design,and property optimization of high-strength steels.展开更多
In this paper we use Böcklund transformation to construct soliton solutions for a coupled KdV system.This system was first proposed by Wang in 2010.First we generalize the well-known Bäcklund transformation ...In this paper we use Böcklund transformation to construct soliton solutions for a coupled KdV system.This system was first proposed by Wang in 2010.First we generalize the well-known Bäcklund transformation for the KdV equation to such coupled KdV system.Then from a trivial seed solution,we construct soliton solutions.We also give a nonlinear superposition formula,which allows us to generate multi-soliton solutions.展开更多
The efforts Xiamen has made in ecological protection over the last 20 years have helped it become a beautiful and clean city.TWENTY years ago,I had the opportunity to travel to the city of Xiamen in southeastern China...The efforts Xiamen has made in ecological protection over the last 20 years have helped it become a beautiful and clean city.TWENTY years ago,I had the opportunity to travel to the city of Xiamen in southeastern China’s Fujian Province.Unfortunately,due to the fact that I was in a rush,I did not have the chance to see the whole city.My memory of Xiamen was its pleasant climate and unforgettable beauty-I knew if I ever got the opportunity,I would revisit this place.Twenty years went by until I was able to return to this city again.After emerging from the airport,I realized that I had grown older,while Xiamen was becoming increasingly youthful and vibrant.What exactly had given this city such vitality?展开更多
BACKGROUND Malignant transformation(MT)of mature cystic teratoma(MCT)has a poor prognosis,especially in advanced cases.Concurrent chemoradiotherapy(CCRT)has an inhibitory effect on MT.CASE SUMMARY Herein,we present a ...BACKGROUND Malignant transformation(MT)of mature cystic teratoma(MCT)has a poor prognosis,especially in advanced cases.Concurrent chemoradiotherapy(CCRT)has an inhibitory effect on MT.CASE SUMMARY Herein,we present a case in which CCRT had a reduction effect preoperatively.A 73-year-old woman with pyelonephritis was referred to our hospital.Computed tomography revealed right hydronephrosis and a 6-cm pelvic mass.Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)revealed squamous cell carci-noma.The patient was diagnosed with MT of MCT.Due to her poor general con-dition and renal malfunction,we selected CCRT,expecting fewer adverse effects.After CCRT,her performance status improved,and the tumor size was reduced;surgery was performed.Five months postoperatively,the patient developed dis-semination and lymph node metastases.Palliative chemotherapy was ineffective.She died 18 months after treatment initiation.CONCLUSION EUS-FNB was useful in the diagnosis of MT of MCT;CCRT suppressed the disea-se and improved quality of life.展开更多
Objective: To develop a best-evidence-based optimal nutrition management plan for patients with chronic heart failure, apply it in clinical practice, and evaluate its effectiveness. Methods: Use the KTA knowledge tran...Objective: To develop a best-evidence-based optimal nutrition management plan for patients with chronic heart failure, apply it in clinical practice, and evaluate its effectiveness. Methods: Use the KTA knowledge translation model to guide evidence-based practice in nutrition management, and compare the nutritional status, cardiac function status, quality of life, and quality review indicators of chronic heart failure patients before and after the application of evidence. Results: After the application of evidence, the nutritional status indicators (MNA-SF score, albumin, hemoglobin) of two groups of heart failure patients significantly increased compared to before the application of evidence, with statistically significant differences (p Conclusion: The KTA knowledge translation model provides methodological guidance for the implementation of evidence-based practice for heart failure patients. This evidence-based practice project is beneficial for improving the outcomes of malnutrition in chronic heart failure patients and is conducive to standardizing nursing pathways, thereby promoting the improvement of nursing quality.展开更多
In this paper, we get the N-fold Darboux transformation with multi-parameters for the coupled mKdV equations with the help of a guage transformation of the spectral problem. As an application, some new multi-soliton s...In this paper, we get the N-fold Darboux transformation with multi-parameters for the coupled mKdV equations with the help of a guage transformation of the spectral problem. As an application, some new multi-soliton solutions and complexiton solutions are obtained from choosing the appropriate seed solution. All obtained solutions and N-fold Darboux transformations are expressed using the Vandermonde-like determinants.展开更多
The digital economy has become an important impetus of global economic growth. The rapid development of emerging and key technological fields, such as the new generation of information technology and biotechnology, ha...The digital economy has become an important impetus of global economic growth. The rapid development of emerging and key technological fields, such as the new generation of information technology and biotechnology, has put forward new requirements for standards development.However, the traditional mode of standards development relying on paper documents faces problems such as low efficiency, long cycle, and relatively low quality, which fails to meet current demands.展开更多
In the context of the digital transformation of vocational education,a quality evaluation index system has been constructed.Based on a questionnaire survey conducted among higher vocational colleges and enterprises in...In the context of the digital transformation of vocational education,a quality evaluation index system has been constructed.Based on a questionnaire survey conducted among higher vocational colleges and enterprises in Hainan Province,it has been found that the quality of vocational education generally depends on the talent training program and professional construction at the macro level.At the meso level,the teacher level and teaching environment are critical,while at the micro level,the evaluation of talent training quality cannot be underestimated.Strategies for quality improvement in vocational education are proposed from the perspectives of talent training programs,major construction,teacher development,teaching environment,and talent training quality,all under the lens of digital transformation.展开更多
With the advancement of information technology,the digital transformation of higher education has become a global trend in educational reform.Egypt has propelled the modernization of its higher education system throug...With the advancement of information technology,the digital transformation of higher education has become a global trend in educational reform.Egypt has propelled the modernization of its higher education system through digital means,focusing on enhancing teachers’digital teaching capabilities,improving course quality,and establishing a policy environment that supports digital instruction.The driving forces behind Egypt’s transformation include meeting the demand for skilled talent due to economic reforms,participating in internationalization processes,and the impetus provided by public diplomacy.By introducing high-quality international resources,deepening scientific research collaboration,and applying digital technologies,Egypt has effectively alleviated issues of resource insufficiency and uneven development,thereby enhancing its capacity for higher education quality assurance.Egypt’s practice demonstrates that digitalization is a key pathway for developing countries to improve the quality of higher education,providing valuable insights for constructing sustainable models of higher education development.展开更多
The combination and application of information technology and higher education have led to changes in the form and mode of teaching management in higher education,promoting the continuous deepening of digital transfor...The combination and application of information technology and higher education have led to changes in the form and mode of teaching management in higher education,promoting the continuous deepening of digital transformation in higher education.This paper discusses higher education management innovation triggered by digital technology and the new appearance of digital university governance,digital evaluation management,and internal governance of higher education.From the perspective of forging new forms,models,and paths of digital management through digital technology,this paper focuses on analyzing the reshaping of management systems by digitalization in higher education.The promotion and inspiration of digitalization on management system reform are provided,including typical features brought about,new paths opened up,and new advantages shaped.It is emphasized that the establishment of digital management system needs to rely on the systematic integration of multiple aspects.Finally,the challenges and solutions faced by the digital management system are deeply analyzed from the aspects of collaborative management and services,management process interoperability,evaluation and trust protection,and personnel digital literacy.Digital technology drives the transformation of higher education management system,resulting in new forms,models,paths,and methods of networked and digital management based on intelligent analysis and processing of educational and teaching data and promoting higher education management toward digital governance.The comprehensive establishment of a digital management system will unleash strong potential for universities to better fulfill their functions,and the high-quality development of higher education will be more solid and powerful.展开更多
ESG(Environmental,Social,and Governance)performance has emerged as a central metric in assessing corporate sustainability.Utilizing ESG rating data for A-share listed companies on the Shanghai Stock Exchange spanning ...ESG(Environmental,Social,and Governance)performance has emerged as a central metric in assessing corporate sustainability.Utilizing ESG rating data for A-share listed companies on the Shanghai Stock Exchange spanning from 2019 to 2022,coupled with measures of corporate transformation and upgrading,this study introduces green innovation as a mediating variable to dissect the impact of ESG ratings on corporate transformation and upgrading.The key findings of this research are as follows:(1)ESG ratings positively influence corporate transformation and upgrading,a conclusion that retains robustness after a comprehensive series of tests and discussions on endogeneity.(2)Mechanism analysis reveals that ESG ratings foster corporate transformation and upgrading by enhancing corporate green innovation.(3)In comparison with other industries,the influence of ESG ratings on corporate transformation and upgrading is notably more pronounced among heavily polluting industries among listed companies in China.Additionally,ESG ratings exhibit a more significant promotional effect on non-state-owned enterprises compared to state-owned enterprises.Larger enterprises play a more substantial role in transformation and upgrading than small and medium-sized enterprises.The promotional effect of ESG ratings is more evident in enterprises with poor information disclosure quality.Furthermore,as media attention increases,so does the impact of ESG ratings on corporate transformation.This study offers valuable policy insights from the ESG rating perspective,aiming to propel corporate transformation and upgrading,thereby contributing to economic high-quality and sustainable development.展开更多
Litchi has great economic significance as a global fruit crop.However,the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation.Here,we prese...Litchi has great economic significance as a global fruit crop.However,the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation.Here,we present an efficacious Agrobacterium tumefaciens-mediated transformation system in somatic embryos of‘Heiye'litchi.This system was developed through the optimization of key variables encompassing explant selection,A.tumefaciens strain delineation,bacterium concentration,infection duration,and infection methodology.The subsequent validation of the transformation technique in litchi was realized through the ectopic expression of LcMYB1,resulting in the generation of transgenic calli.However,the differentiation of transgenic calli into somatic embryos encountered substantial challenges.To delineate the intricate molecular underpinnings of LcMYB1's inhibitory role in somatic embryo induction,a comprehensive transcriptome analysis was conducted that encompassed embryogenic calli(C),globular embryos(G),and transgenic calli(TC).A total of 1,166 common differentially expressed genes(DEGs)were identified between C-vs.-G and C-vs.-TC.Gene Ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that these common DEGs were mostly related to plant hormone signal transduction pathways.Furthermore,RT-qPCR corroborated the pronounced down-regulation of numerous genes that are associated with somatic embryo induction within the transgenic calli.The development of this transformation system provides valuable support for functional genomics research in litchi.展开更多
Under the guidance of national policies,the“coal to gas”project has become one of the important measures to promote the transformation of energy structure in China.Foshan,an important industrial town in Guangdong pr...Under the guidance of national policies,the“coal to gas”project has become one of the important measures to promote the transformation of energy structure in China.Foshan,an important industrial town in Guangdong province,the implementation of the“coal to gas”policy has demonstrated significance for the optimization of the national energy structure.Through the analysis of the change in Foshan’s energy consumption structure and the implementation of the policy,this paper found that there were some challenges in the policy implementation process,such as high economic cost,lagging infrastructure,low social acceptance,and lack of technical personnel.To address this,suggestions are put forward,including increasing financial subsidies,improving laws and regulations,promoting technological innovation,and encouraging social inclusion policies.These recommendations aim to provide guidance and reference for the remaining“coal to gas”projects and the development of clean energy.展开更多
The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficie...The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable transformation.This study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus species.Hairy roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus amarus.Employing a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus species.Notably,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no mutations.Among the 31 different mutation types identified at the sgRNA1 site,base deletion was the most prevalent.Using the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were confirmed.These findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.展开更多
With respect to oceanic fluid dynamics,certain models have appeared,e.g.,an extended time-dependent(3+1)-dimensional shallow water wave equation in an ocean or a river,which we investigate in this paper.Using symbolic...With respect to oceanic fluid dynamics,certain models have appeared,e.g.,an extended time-dependent(3+1)-dimensional shallow water wave equation in an ocean or a river,which we investigate in this paper.Using symbolic computation,we find out,on one hand,a set of bilinear auto-Backlund transformations,which could connect certain solutions of that equation with other solutions of that equation itself,and on the other hand,a set of similarity reductions,which could go from that equation to a known ordinary differential equation.The results in this paper depend on all the oceanic variable coefficients in that equation.展开更多
基金supported by the National Natural Science Foundation of China(No.52271089)the financial support from the C hina Postdoctoral Science Foundation(No.2023M732192)。
文摘This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic analyses.The results show that the Co-bearing steel exhibits finer blocks and a lower ductile-brittle transition temperature than the steel without Co.Moreover,the Co-bearing steel reveals higher transformation rates at the intermediate stage with bainite volume fraction ranging from around 0.1 to 0.6.The improved impact toughness of the Co-bearing steel results from the higher dense block boundaries dominated by the V1/V2 variant pair.Furthermore,the addition of Co induces a larger transformation driving force and a lower bainite start temperature(BS),thereby contributing to the refinement of blocks and the increase of the V1/V2 variant pair.These findings would be instructive for the composition,microstructure design,and property optimization of high-strength steels.
基金Supported by the Jiangsu Higher School Undergraduate Innovation and Entrepreneurship Training Program(202311117078Y)。
文摘In this paper we use Böcklund transformation to construct soliton solutions for a coupled KdV system.This system was first proposed by Wang in 2010.First we generalize the well-known Bäcklund transformation for the KdV equation to such coupled KdV system.Then from a trivial seed solution,we construct soliton solutions.We also give a nonlinear superposition formula,which allows us to generate multi-soliton solutions.
文摘The efforts Xiamen has made in ecological protection over the last 20 years have helped it become a beautiful and clean city.TWENTY years ago,I had the opportunity to travel to the city of Xiamen in southeastern China’s Fujian Province.Unfortunately,due to the fact that I was in a rush,I did not have the chance to see the whole city.My memory of Xiamen was its pleasant climate and unforgettable beauty-I knew if I ever got the opportunity,I would revisit this place.Twenty years went by until I was able to return to this city again.After emerging from the airport,I realized that I had grown older,while Xiamen was becoming increasingly youthful and vibrant.What exactly had given this city such vitality?
文摘BACKGROUND Malignant transformation(MT)of mature cystic teratoma(MCT)has a poor prognosis,especially in advanced cases.Concurrent chemoradiotherapy(CCRT)has an inhibitory effect on MT.CASE SUMMARY Herein,we present a case in which CCRT had a reduction effect preoperatively.A 73-year-old woman with pyelonephritis was referred to our hospital.Computed tomography revealed right hydronephrosis and a 6-cm pelvic mass.Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)revealed squamous cell carci-noma.The patient was diagnosed with MT of MCT.Due to her poor general con-dition and renal malfunction,we selected CCRT,expecting fewer adverse effects.After CCRT,her performance status improved,and the tumor size was reduced;surgery was performed.Five months postoperatively,the patient developed dis-semination and lymph node metastases.Palliative chemotherapy was ineffective.She died 18 months after treatment initiation.CONCLUSION EUS-FNB was useful in the diagnosis of MT of MCT;CCRT suppressed the disea-se and improved quality of life.
文摘Objective: To develop a best-evidence-based optimal nutrition management plan for patients with chronic heart failure, apply it in clinical practice, and evaluate its effectiveness. Methods: Use the KTA knowledge translation model to guide evidence-based practice in nutrition management, and compare the nutritional status, cardiac function status, quality of life, and quality review indicators of chronic heart failure patients before and after the application of evidence. Results: After the application of evidence, the nutritional status indicators (MNA-SF score, albumin, hemoglobin) of two groups of heart failure patients significantly increased compared to before the application of evidence, with statistically significant differences (p Conclusion: The KTA knowledge translation model provides methodological guidance for the implementation of evidence-based practice for heart failure patients. This evidence-based practice project is beneficial for improving the outcomes of malnutrition in chronic heart failure patients and is conducive to standardizing nursing pathways, thereby promoting the improvement of nursing quality.
文摘In this paper, we get the N-fold Darboux transformation with multi-parameters for the coupled mKdV equations with the help of a guage transformation of the spectral problem. As an application, some new multi-soliton solutions and complexiton solutions are obtained from choosing the appropriate seed solution. All obtained solutions and N-fold Darboux transformations are expressed using the Vandermonde-like determinants.
文摘The digital economy has become an important impetus of global economic growth. The rapid development of emerging and key technological fields, such as the new generation of information technology and biotechnology, has put forward new requirements for standards development.However, the traditional mode of standards development relying on paper documents faces problems such as low efficiency, long cycle, and relatively low quality, which fails to meet current demands.
文摘In the context of the digital transformation of vocational education,a quality evaluation index system has been constructed.Based on a questionnaire survey conducted among higher vocational colleges and enterprises in Hainan Province,it has been found that the quality of vocational education generally depends on the talent training program and professional construction at the macro level.At the meso level,the teacher level and teaching environment are critical,while at the micro level,the evaluation of talent training quality cannot be underestimated.Strategies for quality improvement in vocational education are proposed from the perspectives of talent training programs,major construction,teacher development,teaching environment,and talent training quality,all under the lens of digital transformation.
基金The 2024 Northwest University Graduate Innovation Project“Research on the Public Diplomacy Policy of the United States towards North Africa in the Post 9/11 Era and Its Image Shaping of the United States”(2000 present)(CX2024078)。
文摘With the advancement of information technology,the digital transformation of higher education has become a global trend in educational reform.Egypt has propelled the modernization of its higher education system through digital means,focusing on enhancing teachers’digital teaching capabilities,improving course quality,and establishing a policy environment that supports digital instruction.The driving forces behind Egypt’s transformation include meeting the demand for skilled talent due to economic reforms,participating in internationalization processes,and the impetus provided by public diplomacy.By introducing high-quality international resources,deepening scientific research collaboration,and applying digital technologies,Egypt has effectively alleviated issues of resource insufficiency and uneven development,thereby enhancing its capacity for higher education quality assurance.Egypt’s practice demonstrates that digitalization is a key pathway for developing countries to improve the quality of higher education,providing valuable insights for constructing sustainable models of higher education development.
基金supported by the Project of the 2023 Shandong Province Higher Education Curriculum Ideological and Political Education Reform Research Project“Value Connotation,Intrinsic Logic,and Method Path:Student-centered College Curriculum Ideological and Political Integration‘Salt’in Course Teaching”(SZ2023003)Shandong Provincial Department of Education Key Teaching and Research Project“Ideological and Political Guidance,Goal Orientation,and Multidimensional Integration—Research and Practice of Embedded System Series Curriculum Teaching for New Engineering Majors”(Z2022232)+2 种基金2023 Harbin Institute of Technology(Weihai)Education and Teaching Reform Research Project“Connotation Elements,Related Logic,and Core Measures:Basic Paradigm of Curriculum Construction and Classroom Teaching Reform”(2023MSZZ01)the Key Teaching and Research Project of Shandong Provincial Department of Education,“I+IV”Mentor System—Exploration and Practice of Innovative Talents Training Model for Intelligent Vehicle Engineering New Engineering(Z2023002)the 2024 Harbin Institute of Technology(Weihai)Experimental Teaching and Teaching Laboratory Construction Research Project“Online and Offline Mixing,Virtual-practical Operation Integration,Industrial Technology Guidance—Research and Exploration of New Form Experimental Teaching of Computer Composition Principles”(2024SYZZ03)。
文摘The combination and application of information technology and higher education have led to changes in the form and mode of teaching management in higher education,promoting the continuous deepening of digital transformation in higher education.This paper discusses higher education management innovation triggered by digital technology and the new appearance of digital university governance,digital evaluation management,and internal governance of higher education.From the perspective of forging new forms,models,and paths of digital management through digital technology,this paper focuses on analyzing the reshaping of management systems by digitalization in higher education.The promotion and inspiration of digitalization on management system reform are provided,including typical features brought about,new paths opened up,and new advantages shaped.It is emphasized that the establishment of digital management system needs to rely on the systematic integration of multiple aspects.Finally,the challenges and solutions faced by the digital management system are deeply analyzed from the aspects of collaborative management and services,management process interoperability,evaluation and trust protection,and personnel digital literacy.Digital technology drives the transformation of higher education management system,resulting in new forms,models,paths,and methods of networked and digital management based on intelligent analysis and processing of educational and teaching data and promoting higher education management toward digital governance.The comprehensive establishment of a digital management system will unleash strong potential for universities to better fulfill their functions,and the high-quality development of higher education will be more solid and powerful.
文摘ESG(Environmental,Social,and Governance)performance has emerged as a central metric in assessing corporate sustainability.Utilizing ESG rating data for A-share listed companies on the Shanghai Stock Exchange spanning from 2019 to 2022,coupled with measures of corporate transformation and upgrading,this study introduces green innovation as a mediating variable to dissect the impact of ESG ratings on corporate transformation and upgrading.The key findings of this research are as follows:(1)ESG ratings positively influence corporate transformation and upgrading,a conclusion that retains robustness after a comprehensive series of tests and discussions on endogeneity.(2)Mechanism analysis reveals that ESG ratings foster corporate transformation and upgrading by enhancing corporate green innovation.(3)In comparison with other industries,the influence of ESG ratings on corporate transformation and upgrading is notably more pronounced among heavily polluting industries among listed companies in China.Additionally,ESG ratings exhibit a more significant promotional effect on non-state-owned enterprises compared to state-owned enterprises.Larger enterprises play a more substantial role in transformation and upgrading than small and medium-sized enterprises.The promotional effect of ESG ratings is more evident in enterprises with poor information disclosure quality.Furthermore,as media attention increases,so does the impact of ESG ratings on corporate transformation.This study offers valuable policy insights from the ESG rating perspective,aiming to propel corporate transformation and upgrading,thereby contributing to economic high-quality and sustainable development.
基金supported by the National Natural Science Foundation of China(31872066 and 32272663)the Science and Technology Planning Project of Guangzhou,China(2023B01J2002)+2 种基金the Key Research and Development Program of Hainan,China(ZDYF2023XDNY052)the Seed Industry Engineering Project of Department of Agriculture and Rural Affairs of Guangdong,China(2022-NPY-00-004 and 2022-NBH00-001)the Litchi Industry Science and Technology Special Mission of Yunnan,China(202204BI090021)。
文摘Litchi has great economic significance as a global fruit crop.However,the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation.Here,we present an efficacious Agrobacterium tumefaciens-mediated transformation system in somatic embryos of‘Heiye'litchi.This system was developed through the optimization of key variables encompassing explant selection,A.tumefaciens strain delineation,bacterium concentration,infection duration,and infection methodology.The subsequent validation of the transformation technique in litchi was realized through the ectopic expression of LcMYB1,resulting in the generation of transgenic calli.However,the differentiation of transgenic calli into somatic embryos encountered substantial challenges.To delineate the intricate molecular underpinnings of LcMYB1's inhibitory role in somatic embryo induction,a comprehensive transcriptome analysis was conducted that encompassed embryogenic calli(C),globular embryos(G),and transgenic calli(TC).A total of 1,166 common differentially expressed genes(DEGs)were identified between C-vs.-G and C-vs.-TC.Gene Ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that these common DEGs were mostly related to plant hormone signal transduction pathways.Furthermore,RT-qPCR corroborated the pronounced down-regulation of numerous genes that are associated with somatic embryo induction within the transgenic calli.The development of this transformation system provides valuable support for functional genomics research in litchi.
文摘Under the guidance of national policies,the“coal to gas”project has become one of the important measures to promote the transformation of energy structure in China.Foshan,an important industrial town in Guangdong province,the implementation of the“coal to gas”policy has demonstrated significance for the optimization of the national energy structure.Through the analysis of the change in Foshan’s energy consumption structure and the implementation of the policy,this paper found that there were some challenges in the policy implementation process,such as high economic cost,lagging infrastructure,low social acceptance,and lack of technical personnel.To address this,suggestions are put forward,including increasing financial subsidies,improving laws and regulations,promoting technological innovation,and encouraging social inclusion policies.These recommendations aim to provide guidance and reference for the remaining“coal to gas”projects and the development of clean energy.
基金supported by the Basic Research Funds of Chinese Academy of Agricultural Sciences(Grant No.1610192023201)the National Key Research and Development Program of China(Grant No.SQ2023YFE0201239)+3 种基金China Agriculture Research System of MOF and MARA(Grant No.CARS-25-03)the Major Science and Technology Projects of Henan Province(Grant No.221100110400)the Funding of Joint Research on Agricultural Variety Improvement of Henan Province(Grant No.2022010503)the Agricultural Science and Technology Innovation Program(Grant No.CAAS-ASTIP-2021-ZFRI)。
文摘The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics.Within this system,single guide RNAs(sgRNAs)are instrumental in the precise cleavage of DNA double strands.However,the efficiency of gene editing varies among sgRNAs,emphasizing the need to meticulously select target sites,especially in the context of Citrullus lanatus,a species notorious for its challenging and inefficient generation of transgenic plants through stable transformation.This study employed an Agrobacterium rhizogenes-mediated hairy root method to assess effective target sites for gene editing of ClCIPK17 across various Citrullus species.Hairy roots were successfully induced in different plant tissues at diverse growth stages of Citrullus lanatus,Citrullus mucosospermus,and Citrullus amarus.Employing a vector with two sgRNAs(sgRNA1 and sgRNA5)positioned within conserved regions of exon 1 and exon 5 of ClCIPK17 in the CRISPR/Cas9 system,targeted mutations were detected in 90.9%of accessions across the four Citrullus species.Notably,73.94%of all examined hairy roots exhibited mutations at the sgRNA1 site,while the sgRNA5 site showed no mutations.Among the 31 different mutation types identified at the sgRNA1 site,base deletion was the most prevalent.Using the sgRNA1 site of ClCIPK17,stable transgenic watermelon buds were obtained from explants,and the targeted mutations of the sgRNA1 site were confirmed.These findings underscore the viability of the hairy root transformation system in assessing the editing efficiency of sgRNA targets in diverse Citrullus species.
基金financially supported by the Scientific Research Foundation of North China University of Technology(Grant Nos.11005136024XN147-87 and 110051360024XN151-86).
文摘With respect to oceanic fluid dynamics,certain models have appeared,e.g.,an extended time-dependent(3+1)-dimensional shallow water wave equation in an ocean or a river,which we investigate in this paper.Using symbolic computation,we find out,on one hand,a set of bilinear auto-Backlund transformations,which could connect certain solutions of that equation with other solutions of that equation itself,and on the other hand,a set of similarity reductions,which could go from that equation to a known ordinary differential equation.The results in this paper depend on all the oceanic variable coefficients in that equation.
