【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference fo...【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.展开更多
Paddy fields are considered a major source of methane(CH_4)emissions.Aerobic irrigation methods have proven to be efficacious in mitigating CH_4 emissions in paddy cultivation.The promising role of compound microbial ...Paddy fields are considered a major source of methane(CH_4)emissions.Aerobic irrigation methods have proven to be efficacious in mitigating CH_4 emissions in paddy cultivation.The promising role of compound microbial agents in refining the rhizospheric ecosystem suggests their potential as novel agents in reducing CH_4 emissions from paddy fields.To explore a new method of using compound microbial agents to reduce CH_4 emissions,we conducted pot and field experiments over the period of 2022-2023.We measured CH_4 flux,the redox potential(Eh)of the soil,the concentration of dissolved oxygen(DO)in the floodwater,and the gene abundance of both methanogens(mcr A)and methanotrophs(pmo A).The results showed that the application of the compound microbial agent led to a significant increase in the DO levels within the floodwater and an increase of 9.26%to 35.01%in the Eh of the tillage soil.Furthermore,the abundance of pmo A increased by 31.20%,while the mcr A/pmo A ratio decreased by 25.96%at the maximum tillering stage.Applying 45-75 kg/hm^(2) of the compound microbial agent before transplanting resulted in a reduction of cumulative CH_4 emissions from paddy fields by 17.49%in single-cropped rice and 43.54%to 50.27%in double-cropped late rice during the tillering stage.Correlation analysis indicated that CH_4 flux was significantly negatively correlated with pmo A gene abundance and soil Eh,and positively related to the mcr A/pmo A ratio.Additionally,soil Eh was significantly positively correlated with pmo A gene abundance,suggesting that paddy soil Eh indirectly affected CH_4 flux by influencing the pmo A gene abundance.In conclusion,the pre-planting application of the compound microbial agent at a rate of 45-75 kg/hm^(2) can enhance the Eh in the rhizosphere and increase the abundance of the pmo A gene,thereby reducing CH_4 emissions from paddy fields during the tillering stage of rice growth.展开更多
[ Objectives] The paper was to study effects of different microbial agents on occurrence of tobacco bacterial wilt. [ Methods I Antagonistic strains against Ralstonia solanacearum were screened from in situ soil, and ...[ Objectives] The paper was to study effects of different microbial agents on occurrence of tobacco bacterial wilt. [ Methods I Antagonistic strains against Ralstonia solanacearum were screened from in situ soil, and prepared into four different combinations. The number of soil microorganisms and incidence of bacterial wilt of four combinations were observed. [ Results] The incidence rate of bacterial wilt treated by microbial agent OR-1 was significantly lower than that in control, and the control efficacy against bacterial wilt reached 67%. [ Conclusions] Application of microbial agent OR-1 could effectively reduce the incidence rate and disease index of bacterial wilt, thus improving the quality of tobacco.展开更多
The microbial agent ARC-BBBE demonstration trials were conducted in four provinces in the main peanutproducing areas of the Huang-huai-hai plain of China.The results revealed that the application of ARC-BBBE led to a ...The microbial agent ARC-BBBE demonstration trials were conducted in four provinces in the main peanutproducing areas of the Huang-huai-hai plain of China.The results revealed that the application of ARC-BBBE led to a 1.09–1.70 fold increase in the number of nodules in the treatment group at the demonstration site compared to the control group.Moreover,the nodule weight in the treatment group was 0.80–3.32 times higher than that of the control group,and nitrogenase activity per plant showed a significant enhancement by 1.00–2.83 fold compared to controls.Additionally,notable improvements were observed in terms of increased fresh weight of whole plants,well-filled pod numbers,and enhanced growth performance;ultimately resulting in a harvest yield increase ranging from 9.46%to 49.04%.The abundance of Aspergillus flavus in rhizosphere soil was determined by the dilution spread plate method,and the inhibition rate was up to 86.7%.The application of ARC-BBBE in the significant peanut-producing areas of Huang-huai-hai has effects of promoting growth,nodulation,and increasing production.At the same time,it has the effect of inhibiting and controlling soil Aspergillus flavus,which provides a new green and low-carbon way to promote the high-quality development of the peanut industry.展开更多
To explore the influence of microbial agems on the decomposition of aging dunnage of the fermentation bed, this paper took the aging dunnage as the raw materials, obtained microbial strains of different types through ...To explore the influence of microbial agems on the decomposition of aging dunnage of the fermentation bed, this paper took the aging dunnage as the raw materials, obtained microbial strains of different types through isolation and purification, chose dominant groups to make compound microbial agents, and adopted composting decomposition experiment. The results showed that Bacillus subtilis of different ratios was added, Trichoderma koningii and Thermo actinomycetaceac could promote the decomposition of aging dunnage, especially composite microbial agents （Kc：Kn：Gf = 1：1：1 ） had the best effect of decomposition, the high tem- perature was kept for 13 days. In the end of composting, degradation rate of cellulose, degradation rate of lignin, GI, and C/N were 47.6%, 30. 2%, 98.5%, and 18.5%. Bacillus coli was not detected.展开更多
[ Objective] The aim of this study was to develop a cheap and localized microbial agent so as to solve high cost of microbial agent for pig manure composting in Jiaxing City. [ Method] Pig manure in the experimental g...[ Objective] The aim of this study was to develop a cheap and localized microbial agent so as to solve high cost of microbial agent for pig manure composting in Jiaxing City. [ Method] Pig manure in the experimental group and control group was inoculated with the self-developed micro- bial agent and commercial microbial agent, respectively. The manure was decomposed for 38 d, during which the indicators of compost including physical properties, temperature, pH value, water content, organic matter, dissolved nitrogen, carbon nitrogen ratio and germination rate were studied. [ Result] The water content in the experimental group declined to 26.10% after 33 d of compost, meeting the standard upper limitation of 30% for maturity. By comparison, the water content in the control group was slightly higher than 30% even after 38 d. The germination rate of seeds fertilized with the experimental manure compost met the standard for maturity on Day 28, while that in the control group met the standard for maturity on Day 35. When the composting was finished, the ratio of total carbon to total nitrogen in the pig manure was 14.64 and 16.43 respective- ly in the experimental and control group, and the organic matter content was about 45% for both. All these indexes could meet the standards for or- ganic fertilizer products. [ Conclusion] The self-developed microbial agent can moot the requirements for pig manure composting, and it can shorten the composting time by 5 -8 d compared with the commercial agent. In addition, the fertilizer product composted by the self-developed microbial a qent has lower water content and thereby is much more beneficial for preservation.展开更多
Single or mixed use ofAgaricus bisporus residue and microbial agent had better control effect on cucumber root-knot nematode disease in greenhouse. The results showed that the control effect of single use of A. b/spor...Single or mixed use ofAgaricus bisporus residue and microbial agent had better control effect on cucumber root-knot nematode disease in greenhouse. The results showed that the control effect of single use of A. b/sporus residue was 32.9% - 34.7%, and the yield increasing rate was 2.1% - 8.7% ; the control effect of mixed use ofA. b/sporus residue and microbial agent was 59. 1% - 63. 6%, and yield increasing rate was 2.6% - 3.5%. The experimental dose had no ad- verse effects on soil physical and chemical properties. The mixed use of A. b/sporus residue and microbial agent was one of the effective ways to control root-knot nematode disease.展开更多
For the control of environment pollution caused by livestock and poultry manure, four microbial strains conducive to aerobic composting were selected and mixed into four compound microbial agents, and effects of diffe...For the control of environment pollution caused by livestock and poultry manure, four microbial strains conducive to aerobic composting were selected and mixed into four compound microbial agents, and effects of different compound microbial agents on aerobic composting of cow manure were studied from aspects of changes in temperature, pH, water content and C/N during the composting process. The results show that microbial agent M2 has the best effect for aerobic composting of cow manure, so microbial agent M2 has broad popularization and application prospect in livestock and poultry farms in low-temperature areas of Northeast China.展开更多
In order to clarify the influence of microbial agents on kitchen waste and rice straw composting, an aerobic composting experiment was conducted by setting three kinds of combined bacterial agents to study the tempera...In order to clarify the influence of microbial agents on kitchen waste and rice straw composting, an aerobic composting experiment was conducted by setting three kinds of combined bacterial agents to study the temperature change in the composting, and pH value, nutrient contents, C/N and heavy metal content after composting through. The three kinds of combined bacterial agents were as follows:B1 with effective strains: Bacillus subtilis, yeast and Trichoderma sp.;B2 with effective strains: Bacillus amyloliquefaciens, yeast and lactic acid bacteria;B3 with effective strains: Bacillus subtilis, yeast and lactic acid bacteria. Results showed that the addition of microbial agents had signifcant effects on temperature change,nitrogen and phosphorus content and C/N of the compost. T1, T2, and T3 treatments lasted for 8, 5 and 4 d in the thermophilic phase above 60℃, respectively. The total nitrogen content of each treatment was 14.90, 15.50 and 13.80 g/kg respectively after composting. The total phosphorus content of each treatment was 4.87, 4.17 and 3.70g/kg respectively at the end of composting. The C/N of each treatment was 20.94,22.63, and 22.65 respectively at the end of composting. The application effect of B1bacteria agent on the composting of kitchen waste and rice straw was better.展开更多
Microbial enhanced oil recovery(MEOR)is a cost effective and efficient method for recovering residual oil.However,the presence of wax(paraffin)in residual oil can substantially reduce the efficiency of MEOR.Therefore,...Microbial enhanced oil recovery(MEOR)is a cost effective and efficient method for recovering residual oil.However,the presence of wax(paraffin)in residual oil can substantially reduce the efficiency of MEOR.Therefore,microbial dewaxing is a critical process in MEOR.In this study,a bacterial dewaxing agent of three spore-forming bacteria was developed.Among these bacteria,Bacillus subtilis GZ6 produced the biosurfactant surfactin.Replacing the promoter of the surfactin synthase gene cluster(srfA),increased the titer of surfactin in this strain from 0.33 g/L to 2.32 g/L.The genetically modified strain produced oil spreading rings with diameters increasing from 3.5±0.1 to 4.1±0.2 cm.The LadA F10L/N133R mutant was created by engineering an alkane monooxygenase(LadA)using site-directed mutagenesis in the Escherichia coli host.Compared to the wild-type enzyme,the resulting mutant exhibited an 11.7-fold increase in catalytic efficiency toward the substrate octadecane.When the mutant(pIMPpladA2mu)was expressed in Geobacillus stearothermophilus GZ178 cells,it exhibited a 2.0-fold increase in octadecane-degrading activity.Cultures of the two modified strains(B.subtilis GZ6(pg3srfA)and G.stearothermophilus GZ178(pIMPpladA2mu))were mixed with the culture of Geobacillus thermodenitrificans GZ156 at a ratio of 5:80:15.The resulting composition increased the rate of wax removal by 35%compared to the composition composed of three native strains.This study successfully developed a multi-strain bacterial agent with enhanced oil wax removal capabilities by genetically engineering two bacterial strains.展开更多
Although composting is a very effective way to dispose agricultural wastes,its development is greatly limited by the low compost quality and greenhouse gas emissions.At present,there is a lack of effective means to so...Although composting is a very effective way to dispose agricultural wastes,its development is greatly limited by the low compost quality and greenhouse gas emissions.At present,there is a lack of effective means to solve these two problems simultaneously.Here,the effects of three additives of compound microbial agent,biochar and biochar carried microbial agent on the composting performance,nitrogen transformation,greenhouse gas and ammonia emissions,and bacterial communities were investigated in sheep manure composting during 28 days.Results showed that biochar carried microbial agent prolonged the thermophilic stage and promoted compost maturity.At the same time,it was confirmed by the increase of the decomposition of organic nitrogen and the transformation of NH_(4)^(+)-N to NO_(3)^(−)-N.Besides,adding biochar carried microbial agent decreased CH_(4),NH_(3) and N_(2)O emissions by 65.23%,42.05%and 68.64%,respectively.The gas emissions were mainly correlated to Chloroflexi,Myxococcota,Acidobacteriota,Firmicutes,and Gemmatimonadota.Redundancy analysis showed that EC and TKN were closely related to bacterial community.Therefore,biochar carried microbial agent is recommended as an effective additive to enhance compost quality and reduce gas emissions during sheep manure composting.展开更多
Microbial inoculation agent has been widely used in waste disposal and waste recycling. In this paper, the principle of fermentation in the composting of agricultural waste and the impact of microbial inoculation agen...Microbial inoculation agent has been widely used in waste disposal and waste recycling. In this paper, the principle of fermentation in the composting of agricultural waste and the impact of microbial inoculation agent on composting were reviewed.The research and the application of microbial inoculation agent were also introduced. Based on the trend of the research, the promising prospect of microbial inoculation agent was discussed.展开更多
Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several st...Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several strategies to fortify the food supply system to a high food safety standard. This relies heavily on laboratory testing services but conventional methods for detection of food contaminants and toxicants are limited by sophisticated sample preparation procedures, long analysis time, large instruments and professional personnel to meet the increasing demands. In this review, we have incorporated most of the current and potential rapid detection methods for many notorious food contaminants and toxicants including microbial agents, toxic ions, pesticides, veterinary drugs and preservatives, as well as detection of genetically modified food genes and adulterated edible oil. Development of rapid, accurate, easy-to-use and affordable testing methods could urge food handlers and the public to actively screen for food contaminants and toxicants instead of passively relying on monitoring by the government examination facility. This review also provides several recommendations including how to encourage the public to engage in the food safety management system and provide optimal education and financial assistance that may improve the current Chinese food safety control system.展开更多
[ Objective] The study aimed to explore the function mechanism of microbial ecological agent DUAN-NAI-AN on controlling weaned piglet diarrhea. E Method] DUAN-NAI-AN was given to the weaned piglets by oral administrat...[ Objective] The study aimed to explore the function mechanism of microbial ecological agent DUAN-NAI-AN on controlling weaned piglet diarrhea. E Method] DUAN-NAI-AN was given to the weaned piglets by oral administration and its effects on diarrhea rate, growth performance, in- testinal microflora, pH values of different parts of the intestine and intestinal mucosal morphology were observed. [ Result] DUAN-NAI-AN could im- prove the growth performance of weaned piglets by increasing the growth rate and phase weight gain, showing as higher livability, average daily gain and daily feed intake as well as lower diarrhea rate with 19.24% decline by contrast to the control. In addition, DUAN-NAI-AN could keep the intestinal mucosal mechanical barrier and normal digestive function by strengthening intestinal resistance and thus enhance the intestinal mucosal im- mune level. It could also decrease the proportion of potential pathogens and their toxicity effects inside the intestine to help the piglets transfer to and then maintain the dynamic balance of intestinal microflora in a very short period, and it also showed a trend of decreasing the pH value of piglet in- testinal contents. [ Conclusion] DUAN-NAI-AN is an effective microbial ecological agent for the control of weaned piglet diarrhea.展开更多
Microbial activity and interaction are the important driving factors in the start-up phase of food waste composting at low temperature.The aim of this study was to explore the effect of inoculating Bacillus lichenifor...Microbial activity and interaction are the important driving factors in the start-up phase of food waste composting at low temperature.The aim of this study was to explore the effect of inoculating Bacillus licheniformis on the degradation of organic components and the potential microbe-driven mechanism from the aspects of organic matter degradation,enzyme activity,microbial community interaction,and microbial metabolic function.The results showed that after inoculating B.licheniformis,temperature increased to 47.8℃ on day2,and the degradation of readily degraded carbohydrates(RDC)increased by 31.2%,and the bioheat production increased by 16.5%.There was an obvious enhancement of extracellular enzymes activities after inoculation,especially amylase activity,which increased by 7.68 times on day 4.The inoculated B.licheniformis colonized in composting as key genus in the start-up phase.Modular network analysis and Mantel test indicated that inoculation drove the cooperation between microbial network modules who were responsible for various organic components(RDC,lipid,protein,and lignocellulose)degradation in the start-up phase.Metabolic function prediction suggested that carbohydrate metabolisms including starch and sucrose metabolism,glycolysis/gluconeogenesis,pyruvate metabolism,etc.,were improved by increasing the abundance of related functional genes after inoculation.In conclusion,inoculating B.licheniformis accelerated organic degradation by driving the cooperation between microbial network modules and enhancing microbial metabolism in the start-up phase of composting.展开更多
Cement-based materials are brittle and crack easily under natural conditions.Cracks can reduce service life because the transport of harmful substances can cause corrosion damage to the structures.This review discusse...Cement-based materials are brittle and crack easily under natural conditions.Cracks can reduce service life because the transport of harmful substances can cause corrosion damage to the structures.This review discusses the feasibility of using microbial self-healing agents for crack healing.Tubular and spherical carriers can be used to load microbial self-healing agents and protect microbes,which prolongs the self-healing time.The area self-healing ratio,permeability,mechanical strength,precipitation depth method,numerical modeling,and ultrasonic method can be employed to identify the self-healing effect of cracks.Moreover,the self-healing mechanism is systematically analyzed.The results showed that microbial self-healing agents can repair cracks in cement-based materials in underground projects and dam gates.The difficulties and future development of self-healing cracks were analyzed.A microbial selfhealing agent was embedded in the cement-based material,which automatically repaired the developing cracks.With the development of intelligent building materials,self-healing cracks have become the focus of attention.展开更多
The expected rise in temperature and decreased precipitation owing to climate change and unabated anthropogenic activities add complexity and uncertainty to agro-industry. The impact of soil nutrient imbalance, misman...The expected rise in temperature and decreased precipitation owing to climate change and unabated anthropogenic activities add complexity and uncertainty to agro-industry. The impact of soil nutrient imbalance, mismanaged use of chemicals, high temperature, flood or drought, soil salinity, and heavy metal pollutions, with regard to food security, is increasingly being explored worldwide. This review describes the role of soil-plant-microbe interactions along with organic manure in solving stressed agriculture problems. Beneficial microbes associated with plants are known to stimulate plant growth and enhance plant resistance to biotic （diseases） and abiotic （salinity, drought, pollutions, etc.） stresses. The plant growth-promoting rhizobemteria （PGPR） and mycorrhizae, a key component of soil microbiota, could play vital roles in the maintenance of plant fitness and soil health under stressed environments. The application of organic manure as a soil conditioner to stressed soils along with suitable microbial strains could further enhance the plant-microbe associations and increase the crop yield. A combination of plant, stress-tolerant microbe, and organic amendment represents the tripartite association to offer a favourable environment to the proliferation of beneficial rhizosphere microbes that in turn enhance the plant growth performance in disturbed agro-ecosystem. Agriculture land use patterns with the proper exploitation of plant-microbe associations, with compatible beneficial microbial agents, could be one of the most effective strategies in the management of the concerned agriculture lands owing to climate change resilience. However, the association of such microbes with plants for stressed agriculture management still needs to be explored in greater depth.展开更多
基金Key Research project of Gansu Province of China(22YF7NA108)National Natural Science Foundation of China(31860549)+1 种基金Industry Supporting Project from Education Department of Gansu Province(2023CYZC-49)Major Science and Technology project of Gansu province(24ZDNA006)。
文摘【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.
基金supported by the Zhejiang‘Ten Thousand Talents’Plan Science and Technology Innovation Leading Talent Project,China (Grant No.2020R52035)the National Rice Industry Technology System of China (Grant No.CARS-01-31)the Agricultural Science and Technology Innovation Program,China (Grant No.CAAS-ZDRW202001)。
文摘Paddy fields are considered a major source of methane(CH_4)emissions.Aerobic irrigation methods have proven to be efficacious in mitigating CH_4 emissions in paddy cultivation.The promising role of compound microbial agents in refining the rhizospheric ecosystem suggests their potential as novel agents in reducing CH_4 emissions from paddy fields.To explore a new method of using compound microbial agents to reduce CH_4 emissions,we conducted pot and field experiments over the period of 2022-2023.We measured CH_4 flux,the redox potential(Eh)of the soil,the concentration of dissolved oxygen(DO)in the floodwater,and the gene abundance of both methanogens(mcr A)and methanotrophs(pmo A).The results showed that the application of the compound microbial agent led to a significant increase in the DO levels within the floodwater and an increase of 9.26%to 35.01%in the Eh of the tillage soil.Furthermore,the abundance of pmo A increased by 31.20%,while the mcr A/pmo A ratio decreased by 25.96%at the maximum tillering stage.Applying 45-75 kg/hm^(2) of the compound microbial agent before transplanting resulted in a reduction of cumulative CH_4 emissions from paddy fields by 17.49%in single-cropped rice and 43.54%to 50.27%in double-cropped late rice during the tillering stage.Correlation analysis indicated that CH_4 flux was significantly negatively correlated with pmo A gene abundance and soil Eh,and positively related to the mcr A/pmo A ratio.Additionally,soil Eh was significantly positively correlated with pmo A gene abundance,suggesting that paddy soil Eh indirectly affected CH_4 flux by influencing the pmo A gene abundance.In conclusion,the pre-planting application of the compound microbial agent at a rate of 45-75 kg/hm^(2) can enhance the Eh in the rhizosphere and increase the abundance of the pmo A gene,thereby reducing CH_4 emissions from paddy fields during the tillering stage of rice growth.
基金Supported by Science and Technology Project of Nanping Branch of Fujian Tobacco Company(NYK2012-14-3)
文摘[ Objectives] The paper was to study effects of different microbial agents on occurrence of tobacco bacterial wilt. [ Methods I Antagonistic strains against Ralstonia solanacearum were screened from in situ soil, and prepared into four different combinations. The number of soil microorganisms and incidence of bacterial wilt of four combinations were observed. [ Results] The incidence rate of bacterial wilt treated by microbial agent OR-1 was significantly lower than that in control, and the control efficacy against bacterial wilt reached 67%. [ Conclusions] Application of microbial agent OR-1 could effectively reduce the incidence rate and disease index of bacterial wilt, thus improving the quality of tobacco.
基金The authors thank the National Natural Sciences Foundation of China(32030085,U22A20551)Major project of Hubei Hongshan Laboratory(2021hszd015)+1 种基金Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP2021-OCRI)Knowledge Innovation Program of Wuhan-Shuguang Project(3570).
文摘The microbial agent ARC-BBBE demonstration trials were conducted in four provinces in the main peanutproducing areas of the Huang-huai-hai plain of China.The results revealed that the application of ARC-BBBE led to a 1.09–1.70 fold increase in the number of nodules in the treatment group at the demonstration site compared to the control group.Moreover,the nodule weight in the treatment group was 0.80–3.32 times higher than that of the control group,and nitrogenase activity per plant showed a significant enhancement by 1.00–2.83 fold compared to controls.Additionally,notable improvements were observed in terms of increased fresh weight of whole plants,well-filled pod numbers,and enhanced growth performance;ultimately resulting in a harvest yield increase ranging from 9.46%to 49.04%.The abundance of Aspergillus flavus in rhizosphere soil was determined by the dilution spread plate method,and the inhibition rate was up to 86.7%.The application of ARC-BBBE in the significant peanut-producing areas of Huang-huai-hai has effects of promoting growth,nodulation,and increasing production.At the same time,it has the effect of inhibiting and controlling soil Aspergillus flavus,which provides a new green and low-carbon way to promote the high-quality development of the peanut industry.
基金Sponsored by Hunan Provincial Pig Industry Technology SystemHunan Provincial Program of Agricultural Science and Technology(2012-193)
文摘To explore the influence of microbial agems on the decomposition of aging dunnage of the fermentation bed, this paper took the aging dunnage as the raw materials, obtained microbial strains of different types through isolation and purification, chose dominant groups to make compound microbial agents, and adopted composting decomposition experiment. The results showed that Bacillus subtilis of different ratios was added, Trichoderma koningii and Thermo actinomycetaceac could promote the decomposition of aging dunnage, especially composite microbial agents （Kc：Kn：Gf = 1：1：1 ） had the best effect of decomposition, the high tem- perature was kept for 13 days. In the end of composting, degradation rate of cellulose, degradation rate of lignin, GI, and C/N were 47.6%, 30. 2%, 98.5%, and 18.5%. Bacillus coli was not detected.
基金funded by the Science and Technology Project of Nanhu District,Jiaxing City,Zhejiang Province
文摘[ Objective] The aim of this study was to develop a cheap and localized microbial agent so as to solve high cost of microbial agent for pig manure composting in Jiaxing City. [ Method] Pig manure in the experimental group and control group was inoculated with the self-developed micro- bial agent and commercial microbial agent, respectively. The manure was decomposed for 38 d, during which the indicators of compost including physical properties, temperature, pH value, water content, organic matter, dissolved nitrogen, carbon nitrogen ratio and germination rate were studied. [ Result] The water content in the experimental group declined to 26.10% after 33 d of compost, meeting the standard upper limitation of 30% for maturity. By comparison, the water content in the control group was slightly higher than 30% even after 38 d. The germination rate of seeds fertilized with the experimental manure compost met the standard for maturity on Day 28, while that in the control group met the standard for maturity on Day 35. When the composting was finished, the ratio of total carbon to total nitrogen in the pig manure was 14.64 and 16.43 respective- ly in the experimental and control group, and the organic matter content was about 45% for both. All these indexes could meet the standards for or- ganic fertilizer products. [ Conclusion] The self-developed microbial agent can moot the requirements for pig manure composting, and it can shorten the composting time by 5 -8 d compared with the commercial agent. In addition, the fertilizer product composted by the self-developed microbial a qent has lower water content and thereby is much more beneficial for preservation.
基金Supported by Edible Fungus Industry Innovation Team Projects of Shandong Agricultural Industry Research System(SDAIT-11-011-08)
文摘Single or mixed use ofAgaricus bisporus residue and microbial agent had better control effect on cucumber root-knot nematode disease in greenhouse. The results showed that the control effect of single use of A. b/sporus residue was 32.9% - 34.7%, and the yield increasing rate was 2.1% - 8.7% ; the control effect of mixed use ofA. b/sporus residue and microbial agent was 59. 1% - 63. 6%, and yield increasing rate was 2.6% - 3.5%. The experimental dose had no ad- verse effects on soil physical and chemical properties. The mixed use of A. b/sporus residue and microbial agent was one of the effective ways to control root-knot nematode disease.
基金Supported by the National Science and Technology Project of Water Pollution Control and Management(2012ZX07202-009-002)
文摘For the control of environment pollution caused by livestock and poultry manure, four microbial strains conducive to aerobic composting were selected and mixed into four compound microbial agents, and effects of different compound microbial agents on aerobic composting of cow manure were studied from aspects of changes in temperature, pH, water content and C/N during the composting process. The results show that microbial agent M2 has the best effect for aerobic composting of cow manure, so microbial agent M2 has broad popularization and application prospect in livestock and poultry farms in low-temperature areas of Northeast China.
文摘In order to clarify the influence of microbial agents on kitchen waste and rice straw composting, an aerobic composting experiment was conducted by setting three kinds of combined bacterial agents to study the temperature change in the composting, and pH value, nutrient contents, C/N and heavy metal content after composting through. The three kinds of combined bacterial agents were as follows:B1 with effective strains: Bacillus subtilis, yeast and Trichoderma sp.;B2 with effective strains: Bacillus amyloliquefaciens, yeast and lactic acid bacteria;B3 with effective strains: Bacillus subtilis, yeast and lactic acid bacteria. Results showed that the addition of microbial agents had signifcant effects on temperature change,nitrogen and phosphorus content and C/N of the compost. T1, T2, and T3 treatments lasted for 8, 5 and 4 d in the thermophilic phase above 60℃, respectively. The total nitrogen content of each treatment was 14.90, 15.50 and 13.80 g/kg respectively after composting. The total phosphorus content of each treatment was 4.87, 4.17 and 3.70g/kg respectively at the end of composting. The C/N of each treatment was 20.94,22.63, and 22.65 respectively at the end of composting. The application effect of B1bacteria agent on the composting of kitchen waste and rice straw was better.
基金the financial support from Scientific Research Program of Beijing Municipal Commission of Education(KM202110017009,granted to Xiaoyan Guo)Undergraduates Research Training Program of Beijing Institute of Petrochemical Technology(2022J00178,granted to Lizhu Li)。
文摘Microbial enhanced oil recovery(MEOR)is a cost effective and efficient method for recovering residual oil.However,the presence of wax(paraffin)in residual oil can substantially reduce the efficiency of MEOR.Therefore,microbial dewaxing is a critical process in MEOR.In this study,a bacterial dewaxing agent of three spore-forming bacteria was developed.Among these bacteria,Bacillus subtilis GZ6 produced the biosurfactant surfactin.Replacing the promoter of the surfactin synthase gene cluster(srfA),increased the titer of surfactin in this strain from 0.33 g/L to 2.32 g/L.The genetically modified strain produced oil spreading rings with diameters increasing from 3.5±0.1 to 4.1±0.2 cm.The LadA F10L/N133R mutant was created by engineering an alkane monooxygenase(LadA)using site-directed mutagenesis in the Escherichia coli host.Compared to the wild-type enzyme,the resulting mutant exhibited an 11.7-fold increase in catalytic efficiency toward the substrate octadecane.When the mutant(pIMPpladA2mu)was expressed in Geobacillus stearothermophilus GZ178 cells,it exhibited a 2.0-fold increase in octadecane-degrading activity.Cultures of the two modified strains(B.subtilis GZ6(pg3srfA)and G.stearothermophilus GZ178(pIMPpladA2mu))were mixed with the culture of Geobacillus thermodenitrificans GZ156 at a ratio of 5:80:15.The resulting composition increased the rate of wax removal by 35%compared to the composition composed of three native strains.This study successfully developed a multi-strain bacterial agent with enhanced oil wax removal capabilities by genetically engineering two bacterial strains.
基金National Key Research and Development Program of China(2018YFC1802904)National Science Foundation of China(52264013 and 41867061)+2 种基金Inner Mongolia Science&Technology Plan Program(2019 and 2020)National Science Foundation of Inner Mongolia(2020MS02005)Inner Mongolia Engineering Research Center of Evaluation and Restoration in the Mining Ecological Environment and the Special Fund for the Transformation of Scientific and Technological Achievements in Inner Mongolia(2019CG062).
文摘Although composting is a very effective way to dispose agricultural wastes,its development is greatly limited by the low compost quality and greenhouse gas emissions.At present,there is a lack of effective means to solve these two problems simultaneously.Here,the effects of three additives of compound microbial agent,biochar and biochar carried microbial agent on the composting performance,nitrogen transformation,greenhouse gas and ammonia emissions,and bacterial communities were investigated in sheep manure composting during 28 days.Results showed that biochar carried microbial agent prolonged the thermophilic stage and promoted compost maturity.At the same time,it was confirmed by the increase of the decomposition of organic nitrogen and the transformation of NH_(4)^(+)-N to NO_(3)^(−)-N.Besides,adding biochar carried microbial agent decreased CH_(4),NH_(3) and N_(2)O emissions by 65.23%,42.05%and 68.64%,respectively.The gas emissions were mainly correlated to Chloroflexi,Myxococcota,Acidobacteriota,Firmicutes,and Gemmatimonadota.Redundancy analysis showed that EC and TKN were closely related to bacterial community.Therefore,biochar carried microbial agent is recommended as an effective additive to enhance compost quality and reduce gas emissions during sheep manure composting.
基金Supported by Post-doctoral Management Office of Heilongjiang Province (LBH-Z06161)Key Lab of Utilization and Protection of Black Soil (GXS08-1)
文摘Microbial inoculation agent has been widely used in waste disposal and waste recycling. In this paper, the principle of fermentation in the composting of agricultural waste and the impact of microbial inoculation agent on composting were reviewed.The research and the application of microbial inoculation agent were also introduced. Based on the trend of the research, the promising prospect of microbial inoculation agent was discussed.
文摘Food safety is one of the major concerns in every country regardless of the economic and social development. The frequent occurrence of food scandals in the world has led the Chinese government to implement several strategies to fortify the food supply system to a high food safety standard. This relies heavily on laboratory testing services but conventional methods for detection of food contaminants and toxicants are limited by sophisticated sample preparation procedures, long analysis time, large instruments and professional personnel to meet the increasing demands. In this review, we have incorporated most of the current and potential rapid detection methods for many notorious food contaminants and toxicants including microbial agents, toxic ions, pesticides, veterinary drugs and preservatives, as well as detection of genetically modified food genes and adulterated edible oil. Development of rapid, accurate, easy-to-use and affordable testing methods could urge food handlers and the public to actively screen for food contaminants and toxicants instead of passively relying on monitoring by the government examination facility. This review also provides several recommendations including how to encourage the public to engage in the food safety management system and provide optimal education and financial assistance that may improve the current Chinese food safety control system.
文摘[ Objective] The study aimed to explore the function mechanism of microbial ecological agent DUAN-NAI-AN on controlling weaned piglet diarrhea. E Method] DUAN-NAI-AN was given to the weaned piglets by oral administration and its effects on diarrhea rate, growth performance, in- testinal microflora, pH values of different parts of the intestine and intestinal mucosal morphology were observed. [ Result] DUAN-NAI-AN could im- prove the growth performance of weaned piglets by increasing the growth rate and phase weight gain, showing as higher livability, average daily gain and daily feed intake as well as lower diarrhea rate with 19.24% decline by contrast to the control. In addition, DUAN-NAI-AN could keep the intestinal mucosal mechanical barrier and normal digestive function by strengthening intestinal resistance and thus enhance the intestinal mucosal im- mune level. It could also decrease the proportion of potential pathogens and their toxicity effects inside the intestine to help the piglets transfer to and then maintain the dynamic balance of intestinal microflora in a very short period, and it also showed a trend of decreasing the pH value of piglet in- testinal contents. [ Conclusion] DUAN-NAI-AN is an effective microbial ecological agent for the control of weaned piglet diarrhea.
基金supported by the Open Research Fund Program of State Environmental Protection Key Laboratory of Food Chain Pollution Control(No.FC2022YB01)the National Natural Science Foundation of China(No.32071552)+1 种基金the Independent Research Project of Science and Technology Innovation Base in Tibet Autonomous Region(No.XZ2022JR0007G)China Agricultural University-Dabeinong Group Professional degree graduate joint training reform project(No.CAUDBN PDG-JTRP).
文摘Microbial activity and interaction are the important driving factors in the start-up phase of food waste composting at low temperature.The aim of this study was to explore the effect of inoculating Bacillus licheniformis on the degradation of organic components and the potential microbe-driven mechanism from the aspects of organic matter degradation,enzyme activity,microbial community interaction,and microbial metabolic function.The results showed that after inoculating B.licheniformis,temperature increased to 47.8℃ on day2,and the degradation of readily degraded carbohydrates(RDC)increased by 31.2%,and the bioheat production increased by 16.5%.There was an obvious enhancement of extracellular enzymes activities after inoculation,especially amylase activity,which increased by 7.68 times on day 4.The inoculated B.licheniformis colonized in composting as key genus in the start-up phase.Modular network analysis and Mantel test indicated that inoculation drove the cooperation between microbial network modules who were responsible for various organic components(RDC,lipid,protein,and lignocellulose)degradation in the start-up phase.Metabolic function prediction suggested that carbohydrate metabolisms including starch and sucrose metabolism,glycolysis/gluconeogenesis,pyruvate metabolism,etc.,were improved by increasing the abundance of related functional genes after inoculation.In conclusion,inoculating B.licheniformis accelerated organic degradation by driving the cooperation between microbial network modules and enhancing microbial metabolism in the start-up phase of composting.
基金the National Natural Science Foundation of China(Grant No.51808483)the Natural Science Foundation of Jiangsu Province(No.BK20180930)Opening Funds of the Jiangsu Key Laboratory of Construction Materials(No.CM2018-02).
文摘Cement-based materials are brittle and crack easily under natural conditions.Cracks can reduce service life because the transport of harmful substances can cause corrosion damage to the structures.This review discusses the feasibility of using microbial self-healing agents for crack healing.Tubular and spherical carriers can be used to load microbial self-healing agents and protect microbes,which prolongs the self-healing time.The area self-healing ratio,permeability,mechanical strength,precipitation depth method,numerical modeling,and ultrasonic method can be employed to identify the self-healing effect of cracks.Moreover,the self-healing mechanism is systematically analyzed.The results showed that microbial self-healing agents can repair cracks in cement-based materials in underground projects and dam gates.The difficulties and future development of self-healing cracks were analyzed.A microbial selfhealing agent was embedded in the cement-based material,which automatically repaired the developing cracks.With the development of intelligent building materials,self-healing cracks have become the focus of attention.
文摘The expected rise in temperature and decreased precipitation owing to climate change and unabated anthropogenic activities add complexity and uncertainty to agro-industry. The impact of soil nutrient imbalance, mismanaged use of chemicals, high temperature, flood or drought, soil salinity, and heavy metal pollutions, with regard to food security, is increasingly being explored worldwide. This review describes the role of soil-plant-microbe interactions along with organic manure in solving stressed agriculture problems. Beneficial microbes associated with plants are known to stimulate plant growth and enhance plant resistance to biotic （diseases） and abiotic （salinity, drought, pollutions, etc.） stresses. The plant growth-promoting rhizobemteria （PGPR） and mycorrhizae, a key component of soil microbiota, could play vital roles in the maintenance of plant fitness and soil health under stressed environments. The application of organic manure as a soil conditioner to stressed soils along with suitable microbial strains could further enhance the plant-microbe associations and increase the crop yield. A combination of plant, stress-tolerant microbe, and organic amendment represents the tripartite association to offer a favourable environment to the proliferation of beneficial rhizosphere microbes that in turn enhance the plant growth performance in disturbed agro-ecosystem. Agriculture land use patterns with the proper exploitation of plant-microbe associations, with compatible beneficial microbial agents, could be one of the most effective strategies in the management of the concerned agriculture lands owing to climate change resilience. However, the association of such microbes with plants for stressed agriculture management still needs to be explored in greater depth.
