Objective:To investigate the effects of“Three Methods and Three Acupoints”(TMTP)Tuina therapy on spinal microcirculation in sciatic nerve injury(SNI).Methods:Thirty-six SpragueeDawley rats were randomly assigned to ...Objective:To investigate the effects of“Three Methods and Three Acupoints”(TMTP)Tuina therapy on spinal microcirculation in sciatic nerve injury(SNI).Methods:Thirty-six SpragueeDawley rats were randomly assigned to four groups:normal,sham operation,model,and TMTP Tuina.Successful model induction was confirmed by observable hind limb lameness.After 20 sessions,hind limb grip strength and motor nerve conduction velocity(MNCV)were measured at baseline and following the 10th and 20th intervention.CD31 and a-SMA in the ventral horn of SNI model rats were detected using immunofluorescence.Motor neurons in the ventral horn were detected by Nissl staining.PTEN levels in the ventral horn were measured by ELISA,and PI3K,Akt,BDNF,VEGF,and HIF-1a expression was determined by RT-PCR.Spinal cord microcirculation was evaluated by western blotting analysis of the levels of Akt,p-Akt,BDNF,and VEGF.Results:Hind limb grip strength and MNCV significantly improved in the TMTP Tuina group compared to the model group(both P<.001).Morphology of ventral horn motor neurons in the TMTP Tuina group improved compared to the model group,with increased expressions of a-SMA(P=.002)and CD31(P=.006).Western blot analysis indicated increased expression of VEGF(P=.005),p-Akt(P<.001),and BDNF(P=.008)in the ventral horn following Tuina treatment.RT-PCR analysis revealed increased expression of PI3K,Akt,BDNF,VEGF and HIF-1a(all P<.05).In contrast,expression of PTEN decreased compared to the model group(P<.001).Conclusion:TMTP Tuina therapy may restore motor function in rats,enhance ventral horn motor neuron morphology,and promote angiogenesis and vascular smooth muscle proliferation.The mechanism may involve the activation of the PI3K/Akt signaling pathway.展开更多
Radar data, which have incomparably high temporal and spatial resolution, and lightning data, which are great indicators of severe convection, have been used to improve the initial field and increase the accuracies of...Radar data, which have incomparably high temporal and spatial resolution, and lightning data, which are great indicators of severe convection, have been used to improve the initial field and increase the accuracies of nowcasting and short-term forecasting. Physical initialization combined with the three-dimensional variational data assimilation method(PI3 DVarrh) is used in this study to assimilate two kinds of observation data simultaneously, in which radar data are dominant and lightning data are introduced as constraint conditions. In this way, the advantages of dual observations are adopted. To verify the effect of assimilating radar and lightning data using the PI3 DVarrh method, a severe convective activity that occurred on 5 June 2009 is utilized, and five assimilation experiments are designed based on the Weather Research and Forecasting(WRF) model. The assimilation of radar and lightning data results in moister conditions below cloud top, where severe convection occurs;thus, wet forecasts are generated in this study.The results show that the control experiment has poor prediction accuracy. Radar data assimilation using the PI3 DVarrh method improves the location prediction of reflectivity and precipitation, especially in the last 3-h prediction, although the reflectivity and precipitation are notably overestimated. The introduction of lightning data effectively thins the radar data, reduces the overestimates in radar data assimilation, and results in better spatial pattern and intensity predictions. The predicted graupel mixing ratio is closer to the distribution of the observed lightning,which can provide more accurate lightning warning information.展开更多
基金supported by the National Natural Science Foundation of China(82274675&82074573)the Beijing Natural Science Foundation(7232278).
文摘Objective:To investigate the effects of“Three Methods and Three Acupoints”(TMTP)Tuina therapy on spinal microcirculation in sciatic nerve injury(SNI).Methods:Thirty-six SpragueeDawley rats were randomly assigned to four groups:normal,sham operation,model,and TMTP Tuina.Successful model induction was confirmed by observable hind limb lameness.After 20 sessions,hind limb grip strength and motor nerve conduction velocity(MNCV)were measured at baseline and following the 10th and 20th intervention.CD31 and a-SMA in the ventral horn of SNI model rats were detected using immunofluorescence.Motor neurons in the ventral horn were detected by Nissl staining.PTEN levels in the ventral horn were measured by ELISA,and PI3K,Akt,BDNF,VEGF,and HIF-1a expression was determined by RT-PCR.Spinal cord microcirculation was evaluated by western blotting analysis of the levels of Akt,p-Akt,BDNF,and VEGF.Results:Hind limb grip strength and MNCV significantly improved in the TMTP Tuina group compared to the model group(both P<.001).Morphology of ventral horn motor neurons in the TMTP Tuina group improved compared to the model group,with increased expressions of a-SMA(P=.002)and CD31(P=.006).Western blot analysis indicated increased expression of VEGF(P=.005),p-Akt(P<.001),and BDNF(P=.008)in the ventral horn following Tuina treatment.RT-PCR analysis revealed increased expression of PI3K,Akt,BDNF,VEGF and HIF-1a(all P<.05).In contrast,expression of PTEN decreased compared to the model group(P<.001).Conclusion:TMTP Tuina therapy may restore motor function in rats,enhance ventral horn motor neuron morphology,and promote angiogenesis and vascular smooth muscle proliferation.The mechanism may involve the activation of the PI3K/Akt signaling pathway.
基金the National Key Research and Development Program of China (2017YFC1502102)National Natural Science Youth Fund of China (41905089)。
文摘Radar data, which have incomparably high temporal and spatial resolution, and lightning data, which are great indicators of severe convection, have been used to improve the initial field and increase the accuracies of nowcasting and short-term forecasting. Physical initialization combined with the three-dimensional variational data assimilation method(PI3 DVarrh) is used in this study to assimilate two kinds of observation data simultaneously, in which radar data are dominant and lightning data are introduced as constraint conditions. In this way, the advantages of dual observations are adopted. To verify the effect of assimilating radar and lightning data using the PI3 DVarrh method, a severe convective activity that occurred on 5 June 2009 is utilized, and five assimilation experiments are designed based on the Weather Research and Forecasting(WRF) model. The assimilation of radar and lightning data results in moister conditions below cloud top, where severe convection occurs;thus, wet forecasts are generated in this study.The results show that the control experiment has poor prediction accuracy. Radar data assimilation using the PI3 DVarrh method improves the location prediction of reflectivity and precipitation, especially in the last 3-h prediction, although the reflectivity and precipitation are notably overestimated. The introduction of lightning data effectively thins the radar data, reduces the overestimates in radar data assimilation, and results in better spatial pattern and intensity predictions. The predicted graupel mixing ratio is closer to the distribution of the observed lightning,which can provide more accurate lightning warning information.
