The brain's extracellular matrix(ECM),which is comprised of protein and glycosaminoglycan(GAG)scaffolds,constitutes 20%-40% of the human brain and is considered one of the largest influencers on brain cell functio...The brain's extracellular matrix(ECM),which is comprised of protein and glycosaminoglycan(GAG)scaffolds,constitutes 20%-40% of the human brain and is considered one of the largest influencers on brain cell functioning(Soles et al.,2023).Synthesized by neural and glial cells,the brain's ECM regulates a myriad of homeostatic cellular processes,including neuronal plasticity and firing(Miyata et al.,2012),cation buffering(Moraws ki et al.,2015),and glia-neuron interactions(Anderson et al.,2016).Considering the diversity of functions,dynamic remodeling of the brain's ECM indicates that this understudied medium is an active participant in both normal physiology and neurological diseases.展开更多
Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to p...Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.展开更多
This paper studies the problem of the spectral radius of the uniform hypergraph determined by the signless Laplacian matrix.The upper bound of the spectral radius of a uniform hypergraph is obtained by using Rayleigh ...This paper studies the problem of the spectral radius of the uniform hypergraph determined by the signless Laplacian matrix.The upper bound of the spectral radius of a uniform hypergraph is obtained by using Rayleigh principle and the perturbation of the spectral radius under moving the edge operation,and the extremal hypergraphs are characterized for both supertree and unicyclic hypergraphs.The spectral radius of the graph is generalized.展开更多
An in-depth description of an apparently forgotten matrix operation, the reversal operator, is developed. The properties of such an operation are also given, resulting in a new vector-matrix operation resembling the w...An in-depth description of an apparently forgotten matrix operation, the reversal operator, is developed. The properties of such an operation are also given, resulting in a new vector-matrix operation resembling the well-known ones of conjugation, transposition, and inversion. The reversal operator operates by ordering the object components where applied. Reversal is easy to perform as it is distributive regarding the vector sum and matrix product. Supplementary descriptions of matrix regions not often used in linear algebra, like the anti-diagonal concept, are also discussed. Some practical problems are given.展开更多
Mixed matrix membranes(MMMs)have demonstrated significant promise in energy-intensive gas separations by amalgamating the unique properties of fillers with the facile processability of polymers.However,achieving a sim...Mixed matrix membranes(MMMs)have demonstrated significant promise in energy-intensive gas separations by amalgamating the unique properties of fillers with the facile processability of polymers.However,achieving a simultaneous enhancement of permeability and selectivity remains a formidable challenge,due to the difficulty of achieving an optimal match between polymers and fillers.In this study,we incorporate a porous carbon-based zinc oxide composite(C@ZnO)into high-permeability polymers of intrinsic microporosity(PIMs)to fabricate MMMs.The dipole–dipole interaction between C@ZnO and PIMs ensures their exceptional compatibility,mitigating the formation of non-selective voids in the resulting MMMs.Concurrently,C@ZnO with abundant interconnected pores can provide additional low-resistance pathways for gas transport in MMMs.As a result,the CO_(2) permeability of the optimized C@ZnO/PIM-1 MMMs is elevated to 13,215 barrer,while the CO_(2)/N_(2) and CO_(2)/CH_(4) selectivity reached 21.5 and 14.4,respectively,substantially surpassing the 2008 Robeson upper bound.Additionally,molecular simulation results further corroborate that the augmented membrane gas selectivity is attributed to the superior CO_(2) affinity of C@ZnO.In summary,we believe that this work not only expands the application of MMMs for gas separation but also heralds a paradigm shift in the application of porous carbon materials.展开更多
Capacitive voltage transformers (CVTs) are essential in high-voltage systems. An accurate error assessment is crucial for precise energy metering. However, tracking real-time quantitative changes in capacitive voltage...Capacitive voltage transformers (CVTs) are essential in high-voltage systems. An accurate error assessment is crucial for precise energy metering. However, tracking real-time quantitative changes in capacitive voltage transformer errors, particularly minor variations in multi-channel setups, remains challenging. This paper proposes a method for online error tracking of multi-channel capacitive voltage transformers using a Co-Prediction Matrix. The approach leverages the strong correlation between in-phase channels, particularly the invariance of the signal proportions among them. By establishing a co-prediction matrix based on these proportional relationships, The influence of voltage changes on the primary measurements is mitigated. Analyzing the relationships between the co-prediction matrices over time allows for inferring true measurement errors. Experimental validation with real-world data confirms the effectiveness of the method, demonstrating its capability to continuously track capacitive voltage transformer measurement errors online with precision over extended durations.展开更多
Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug deliv...Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in vivo.Therefore,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic stroke.Matrix metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ischemia.Herein,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel PA-TIMP-QK.PA-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in vitro.The results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor function.These findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.展开更多
Intestinal ischemia-reperfusion injury(IIRI)is a complex and severe pathophysiological process characterized by oxidative stress,inflammation,and apoptosis.In recent years,the critical roles of extracellular matrix(EC...Intestinal ischemia-reperfusion injury(IIRI)is a complex and severe pathophysiological process characterized by oxidative stress,inflammation,and apoptosis.In recent years,the critical roles of extracellular matrix(ECM)genes and microRNAs(miRNAs)in IIRI have garnered widespread attention.This review aims to systematically summarize the diagnostic and therapeutic potential of ECM gene sets and miRNA regulatory networks in IIRI.First,we review the molecular mechanisms of IIRI,focusing on the dual role of the ECM in tissue injury and repair processes.The expression changes and functions of ECM components such as collagen,elastin,and matrix metalloproteinases during IIRI progression are deeply analyzed.Second,we systematically summarize the regulatory roles of miRNAs in IIRI,particularly the mechanisms and functions of miRNAs such as miR-125b and miR-200a in regulating inflammation,apoptosis,and ECM remodeling.Additionally,this review discusses potential diagnostic biomarkers and treatment strategies based on ECM genes and miRNAs.We extensively evaluate the prospects of miRNA-targeted therapy and ECM component modulation in preventing and treating IIRI,emphasizing the clinical translational potential of these emerging therapies.In conclusion,the diagnostic and therapeutic potential of ECM gene sets and miRNA regulatory networks in IIRI provides new directions for further research,necessitating additional clinical and basic studies to validate and expand these findings for improving clinical outcomes in IIRI patients.展开更多
We ourselves recently reviewed our article published in Int J Ophthalmol entitled“Inhibition of RhoA/Rho-kinase pathway suppresses the expression of extracellular matrix induced by CTGF or TGF-βin ARPE-19”and would...We ourselves recently reviewed our article published in Int J Ophthalmol entitled“Inhibition of RhoA/Rho-kinase pathway suppresses the expression of extracellular matrix induced by CTGF or TGF-βin ARPE-19”and would like to submit a correction for the following figures.展开更多
BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors...BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remodeling and the degradation of the ECM.MMP-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific functions.MMP-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of tumors.Elevated MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant cases.AIM To assess the immunohistochemical expression of MMP-9 and MMP-13 in OSCC.METHODS A total of 40 cases with histologically confirmed OSCC by incisional biopsy were included in this cross-sectional retrospective study.The protocols for both MMP-9 and MMP-13 immunohistochemical staining were performed according to the manufacturer’s recommendations along with the normal gingival epithelium as a positive control.All the observations were recorded and Pearson’sχ²test with Fisher exact test was used for statistical analysis.RESULTS Our study showed no significant correlation between MMP-9 and MMP-13 staining intensity and tumor size.The majority of the patients were in advanced TNM stages(III and IV),and showed intense expression of MMP-9 and MMP-13.CONCLUSION The present study suggests that both MMP-9 and MMP-13 play an important and independent role in OSCC progression and invasiveness.Intense expression of MMP-9 and MMP-13,irrespective of histological grade of OSCC,correlates well with TNM stage.Consequently,it is evident that MMP-9 and MMP-13 are important for the invasiveness and progression of tumors.The findings may facilitate the development of new approaches for evaluating lymph node metastases and interventional therapy techniques,hence enhancing the prognosis of patients diagnosed with OSCC.展开更多
Neurodegenerative diseases are a major public health challenge,mainly affecting the elderly population and compromising their cognitive,sensory,and motor functions.Currently,available therapies focus on alleviating sy...Neurodegenerative diseases are a major public health challenge,mainly affecting the elderly population and compromising their cognitive,sensory,and motor functions.Currently,available therapies focus on alleviating symptoms and slowing the progression of these conditions,but they do not yet offer a definitive cure.In this scenario,terpenes emerge as promising natural alternatives due to their neuroprotective properties.These compounds can reduce the formation of protein aggregates,neutralize free radicals,and inhibit pro-inflammatory enzymes,which are crucial factors in the development of neurodegenerative diseases.In addition,terpenes also play an important role in the regulation and remodeling of the extracellular matrix,a key target for improving neuronal functions.Substances such as linalool,pinene,and eugenol,among others,have potential therapeutic effects by modulating inflammatory and oxidative stress processes,the main factors that contribute to the progression of these diseases.Studies suggest that these compounds act on signaling pathways that regulate the extracellular matrix,improving neuronal integrity and,consequently,cognitive and motor function.This work aims to review the potential of terpenes in the treatment of neurodegenerative disorders,with emphasis on their ability to regulate oxidative stress and inflammation,as well as to remodel the extracellular matrix.The interaction between these mechanisms points to the promising use of terpenes as an innovative and natural therapeutic approach to combat these diseases.展开更多
Rehabilitation training is believed to be an effectual strategy that canreduce the risk of dysfunction caused by spasticity.However,achieving visualizationrehabilitation training for patients remains clinically challe...Rehabilitation training is believed to be an effectual strategy that canreduce the risk of dysfunction caused by spasticity.However,achieving visualizationrehabilitation training for patients remains clinically challenging.Herein,wepropose visual rehabilitation training system including iontronic meta-fabrics withskin-friendly and large matrix features,as well as high-resolution image modules fordistribution of human muscle tension.Attributed to the dynamic connection and dissociationof the meta-fabric,the fabric exhibits outstanding tactile sensing properties,such as wide tactile sensing range(0~300 kPa)and high-resolution tactile perception(50 Pa or 0.058%).Meanwhile,thanks to the differential capillary effect,the meta-fabric exhibits a“hitting three birds with one stone”property(dryness wearing experience,long working time and cooling sensing).Based on this,the fabrics can be integrated with garmentsand advanced data analysis systems to manufacture a series of large matrix structure(40×40,1600 sensing units)training devices.Significantly,the tunability of piezo-ionic dynamics of the meta-fabric and the programmability of high-resolution imaging modules allowthis visualization training strategy extendable to various common disease monitoring.Therefore,we believe that our study overcomes theconstraint of standard spasticity rehabilitation training devices in terms of visual display and paves the way for future smart healthcare.展开更多
This paper investigates the development and performance of a new higher-order geometric stiffness matrix that more closely approximates the theoretically derived stiffness coefficients.Factors that influence the accur...This paper investigates the development and performance of a new higher-order geometric stiffness matrix that more closely approximates the theoretically derived stiffness coefficients.Factors that influence the accuracy of the solution are studied using two columns,two braced frames,and one unbraced frame.Discussion is provided when the new geometric stiffness matrix can be used to improve the buckling load analysis results and when it may provide only nominal additional benefit.展开更多
Background: Despite considerable advancements in identifying factors contributing to the development of hepatocellular carcinoma(HCC), the pathogenesis of HCC remains unclear. In many cases, HCC is a consequence of pr...Background: Despite considerable advancements in identifying factors contributing to the development of hepatocellular carcinoma(HCC), the pathogenesis of HCC remains unclear. In many cases, HCC is a consequence of prolonged liver fibrosis, resulting in the formation of an intricate premalignant microenvironment. The accumulation of extracellular matrix(ECM) is a hallmark of premalignant microenvironment. Given the critical role of different matrix components in regulating cell phenotype and function, this study aimed to elucidate the interplay between the fibrotic matrix and malignant features in HCC. Methods: Liver tissues from both control(normal) and carbon tetrachloride(CCl_(4))-induced fibrotic rats were decellularized using sodium dodecyl sulfate(SDS) and Triton X-100. The resulting hydrogel from decellularized ECM was processed into micro-particles via the water-in-oil emulsion method. Microparticles were subsequently incorporated into three-dimensional liver biomimetic micro-tissues(MTs) comprising Huh-7 cells, human umbilical vein endothelial cells(HUVECs), and LX-2 cells. The MTs were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay at day 11, immunofluorescence staining, immunoblotting, and spheroid migration assay at day 14 after co-culture. Results: Fibrotic matrix from CCl4-treated rat livers significantly enhanced the growth rate of the MTs and their expression of CCND1 as compared to the normal one. Fibrotic matrix, also induced the expression of epithelial-to-mesenchymal transition(EMT)-associated genes such as TWIST1, ACTA2, MMP9, CDH2, and VIMENTIN in the MTs as compared to the normal matrix. Conversely, the expression of CDH1 and hepatic maturation genes HNF4A, ALB, CYP3A4 was decreased in the MTs when the fibrotic matrix was used. Furthermore, the fibrotic matrix increased the migration of the MTs and their secretion of alpha-fetoprotein. Conclusions: Our findings suggest a regulatory role for the fibrotic matrix in promoting cancerous phenotype, which could potentially accelerate the progression of malignancy in the liver.展开更多
B2-CuZr phase reinforced amorphous alloy matrix composites has become one of the research hotspots in the field of materials science due to the“transformation-induced plasticity”phenomenon,which makes the composites...B2-CuZr phase reinforced amorphous alloy matrix composites has become one of the research hotspots in the field of materials science due to the“transformation-induced plasticity”phenomenon,which makes the composites show better macroscopic plastic deformability and obvious work-hardening behavior compared to the conventional amorphous alloy matrix composites reinforced with ductile phases.However,the in-situ metastable B2-CuZr phase tends to undergo eutectoid decomposition during solidification,and the volume fraction,size,and distribution of B2-CuZr phase are difficult to control,which limits the development and application of these materials.To date,much efforts have been made to solve the above problems through composition optimization,casting parameter tailoring,and post-processing technique.In this study,a review was given based on relevant studies,focusing on the predictive approach,reinforcing mechanism,and microstructure tailoring methods of B2-CuZr phase reinforced amorphous alloy matrix composites.The research focus and future prospects were also given for the future development of the present composite system.展开更多
BACKGROUND Demineralized bone matrix(DBM)is a commonly utilized allogenic bone graft substitute to promote osseous union.However,little is known regarding outcomes following DBM utilization in foot and ankle surgical ...BACKGROUND Demineralized bone matrix(DBM)is a commonly utilized allogenic bone graft substitute to promote osseous union.However,little is known regarding outcomes following DBM utilization in foot and ankle surgical procedures.AIM To evaluate the clinical and radiographic outcomes following DBM as a biological adjunct in foot and ankle surgical procedures.METHODS During May 2023,the PubMed,EMBASE and Cochrane library databases were systematically reviewed to identify clinical studies examining outcomes following DBM for the management of various foot and ankle pathologies.Data regarding study characteristics,patient demographics,subjective clinical outcomes,radiological outcomes,complications,and failure rates were extracted and analyzed.In addition,the level of evidence(LOE)and quality of evidence(QOE)for each individual study was also assessed.Thirteen studies were included in this review.RESULTS In total,363 patients(397 ankles and feet)received DBM as part of their surgical procedure at a weighted mean follow-up time of 20.8±9.2 months.The most common procedure performed was ankle arthrodesis in 94 patients(25.9%).Other procedures performed included hindfoot fusion,1st metatarsophalangeal joint arthrodesis,5th metatarsal intramedullary screw fixation,hallux valgus correction,osteochondral lesion of the talus repair and unicameral talar cyst resection.The osseous union rate in the ankle and hindfoot arthrodesis cohort,base of the 5th metatarsal cohort,and calcaneal fracture cohort was 85.6%,100%,and 100%,respectively.The weighted mean visual analog scale in the osteochondral lesions of the talus cohort improved from a pre-operative score of 7.6±0.1 to a post-operative score of 0.4±0.1.The overall complication rate was 27.2%,the most common of which was non-union(8.8%).There were 43 failures(10.8%)all of which warranted a further surgical procedure.CONCLUSION This current systematic review demonstrated that the utilization of DBM in foot and ankle surgical procedures led to satisfactory osseous union rates with favorable wound complication rates.Excellent outcomes were observed in patients undergoing fracture fixation augmented with DBM,with mixed evidence supporting the routine use of DBM in fusion procedures of the ankle and hindfoot.However,the low LOE together with the low QOE and significant heterogeneity between the included studies reinforces the need for randomized control trials to be conducted to identify the optimal role of DBM in the setting of foot and ankle surgical procedures.展开更多
The current research of configurable product disassemblability focuses on disassemblability evaluation and disassembly sequence planning. Little work has been done on quantitative analysis of configurable product disa...The current research of configurable product disassemblability focuses on disassemblability evaluation and disassembly sequence planning. Little work has been done on quantitative analysis of configurable product disassemblability. The disassemblability modeling technology for configurable product based on disassembly constraint relation weighted design structure matrix （DSM） is proposed. Major factors affecting the disassemblability of configurable product are analyzed, and the disassembling degrees between components in configurable product are obtained by calculating disassembly entropies such as joint type, joint quantity, disassembly path, disassembly accessibility and material compatibility. The disassembly constraint relation weighted DSM of configurable product is constructed and configuration modules are formed by matrix decomposition and tearing operations. The disassembly constraint relation in configuration modules is strong coupling, and the disassembly constraint relation between modules is weak coupling, and the disassemblability configuration model is constructed based on configuration module. Finally, taking a hydraulic forging press as an example, the decomposed weak coupling components are used as configuration modules alone, components with a strong coupling are aggregated into configuration modules, and the disassembly sequence of components inside configuration modules is optimized by tearing operation. A disassemblability configuration model of the hydraulic forging press is constructed. By researching the disassemblability modeling technology of product configuration design based on disassembly constraint relation weighted DSM, the disassembly property in maintenance, recycling and reuse of configurable product are optimized.展开更多
基金supported by National Institute on Aging(NIH-NIA)R21 AG074152(to KMA)National Institute of Allergy and Infectious Diseases(NIAID)grant DP2 AI171150(to KMA)Department of Defense(DoD)grant AZ210089(to KMA)。
文摘The brain's extracellular matrix(ECM),which is comprised of protein and glycosaminoglycan(GAG)scaffolds,constitutes 20%-40% of the human brain and is considered one of the largest influencers on brain cell functioning(Soles et al.,2023).Synthesized by neural and glial cells,the brain's ECM regulates a myriad of homeostatic cellular processes,including neuronal plasticity and firing(Miyata et al.,2012),cation buffering(Moraws ki et al.,2015),and glia-neuron interactions(Anderson et al.,2016).Considering the diversity of functions,dynamic remodeling of the brain's ECM indicates that this understudied medium is an active participant in both normal physiology and neurological diseases.
基金supported by the Natio`nal Natural Science Foundation of China,No. 81801241a grant from Sichuan Science and Technology Program,No. 2023NSFSC1578Scientific Research Projects of Southwest Medical University,No. 2022ZD002 (all to JX)。
文摘Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.
基金Supported by Natural Science Foundation of HuBei Province(2022CFB299).
文摘This paper studies the problem of the spectral radius of the uniform hypergraph determined by the signless Laplacian matrix.The upper bound of the spectral radius of a uniform hypergraph is obtained by using Rayleigh principle and the perturbation of the spectral radius under moving the edge operation,and the extremal hypergraphs are characterized for both supertree and unicyclic hypergraphs.The spectral radius of the graph is generalized.
文摘An in-depth description of an apparently forgotten matrix operation, the reversal operator, is developed. The properties of such an operation are also given, resulting in a new vector-matrix operation resembling the well-known ones of conjugation, transposition, and inversion. The reversal operator operates by ordering the object components where applied. Reversal is easy to perform as it is distributive regarding the vector sum and matrix product. Supplementary descriptions of matrix regions not often used in linear algebra, like the anti-diagonal concept, are also discussed. Some practical problems are given.
基金financial support from the National Natural Science Foundation of China(Nos.22108258 and 52003251)Program for Science&Technology Innovation Talents in Universities of Henan Province(24HASTIT004)+1 种基金Outstanding Youth Fund of Henan Scientific Committee(222300420085)Science and Technology Joint Project of Henan Province(222301420041)。
文摘Mixed matrix membranes(MMMs)have demonstrated significant promise in energy-intensive gas separations by amalgamating the unique properties of fillers with the facile processability of polymers.However,achieving a simultaneous enhancement of permeability and selectivity remains a formidable challenge,due to the difficulty of achieving an optimal match between polymers and fillers.In this study,we incorporate a porous carbon-based zinc oxide composite(C@ZnO)into high-permeability polymers of intrinsic microporosity(PIMs)to fabricate MMMs.The dipole–dipole interaction between C@ZnO and PIMs ensures their exceptional compatibility,mitigating the formation of non-selective voids in the resulting MMMs.Concurrently,C@ZnO with abundant interconnected pores can provide additional low-resistance pathways for gas transport in MMMs.As a result,the CO_(2) permeability of the optimized C@ZnO/PIM-1 MMMs is elevated to 13,215 barrer,while the CO_(2)/N_(2) and CO_(2)/CH_(4) selectivity reached 21.5 and 14.4,respectively,substantially surpassing the 2008 Robeson upper bound.Additionally,molecular simulation results further corroborate that the augmented membrane gas selectivity is attributed to the superior CO_(2) affinity of C@ZnO.In summary,we believe that this work not only expands the application of MMMs for gas separation but also heralds a paradigm shift in the application of porous carbon materials.
文摘Capacitive voltage transformers (CVTs) are essential in high-voltage systems. An accurate error assessment is crucial for precise energy metering. However, tracking real-time quantitative changes in capacitive voltage transformer errors, particularly minor variations in multi-channel setups, remains challenging. This paper proposes a method for online error tracking of multi-channel capacitive voltage transformers using a Co-Prediction Matrix. The approach leverages the strong correlation between in-phase channels, particularly the invariance of the signal proportions among them. By establishing a co-prediction matrix based on these proportional relationships, The influence of voltage changes on the primary measurements is mitigated. Analyzing the relationships between the co-prediction matrices over time allows for inferring true measurement errors. Experimental validation with real-world data confirms the effectiveness of the method, demonstrating its capability to continuously track capacitive voltage transformer measurement errors online with precision over extended durations.
基金supported by the Natural Science Foundation of Shandong Province,No.ZR2023MC168the National Natural Science Foundation of China,No.31670989the Key R&D Program of Shandong Province,No.2019GSF107037(all to CS).
文摘Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in vivo.Therefore,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic stroke.Matrix metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ischemia.Herein,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel PA-TIMP-QK.PA-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in vitro.The results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor function.These findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.
基金Supported by Health Science and Technology Programme of Zhejiang Province,No.2022KY1391.
文摘Intestinal ischemia-reperfusion injury(IIRI)is a complex and severe pathophysiological process characterized by oxidative stress,inflammation,and apoptosis.In recent years,the critical roles of extracellular matrix(ECM)genes and microRNAs(miRNAs)in IIRI have garnered widespread attention.This review aims to systematically summarize the diagnostic and therapeutic potential of ECM gene sets and miRNA regulatory networks in IIRI.First,we review the molecular mechanisms of IIRI,focusing on the dual role of the ECM in tissue injury and repair processes.The expression changes and functions of ECM components such as collagen,elastin,and matrix metalloproteinases during IIRI progression are deeply analyzed.Second,we systematically summarize the regulatory roles of miRNAs in IIRI,particularly the mechanisms and functions of miRNAs such as miR-125b and miR-200a in regulating inflammation,apoptosis,and ECM remodeling.Additionally,this review discusses potential diagnostic biomarkers and treatment strategies based on ECM genes and miRNAs.We extensively evaluate the prospects of miRNA-targeted therapy and ECM component modulation in preventing and treating IIRI,emphasizing the clinical translational potential of these emerging therapies.In conclusion,the diagnostic and therapeutic potential of ECM gene sets and miRNA regulatory networks in IIRI provides new directions for further research,necessitating additional clinical and basic studies to validate and expand these findings for improving clinical outcomes in IIRI patients.
文摘We ourselves recently reviewed our article published in Int J Ophthalmol entitled“Inhibition of RhoA/Rho-kinase pathway suppresses the expression of extracellular matrix induced by CTGF or TGF-βin ARPE-19”and would like to submit a correction for the following figures.
文摘BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remodeling and the degradation of the ECM.MMP-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific functions.MMP-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of tumors.Elevated MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant cases.AIM To assess the immunohistochemical expression of MMP-9 and MMP-13 in OSCC.METHODS A total of 40 cases with histologically confirmed OSCC by incisional biopsy were included in this cross-sectional retrospective study.The protocols for both MMP-9 and MMP-13 immunohistochemical staining were performed according to the manufacturer’s recommendations along with the normal gingival epithelium as a positive control.All the observations were recorded and Pearson’sχ²test with Fisher exact test was used for statistical analysis.RESULTS Our study showed no significant correlation between MMP-9 and MMP-13 staining intensity and tumor size.The majority of the patients were in advanced TNM stages(III and IV),and showed intense expression of MMP-9 and MMP-13.CONCLUSION The present study suggests that both MMP-9 and MMP-13 play an important and independent role in OSCC progression and invasiveness.Intense expression of MMP-9 and MMP-13,irrespective of histological grade of OSCC,correlates well with TNM stage.Consequently,it is evident that MMP-9 and MMP-13 are important for the invasiveness and progression of tumors.The findings may facilitate the development of new approaches for evaluating lymph node metastases and interventional therapy techniques,hence enhancing the prognosis of patients diagnosed with OSCC.
基金Anderson Nogueira Mendes(#302704/2023-0)is grateful to the public Brazilian agency“Conselho Nacional de Desenvolvimento Científico e Tecnológico”(CNPq)for their personal scholarships.
文摘Neurodegenerative diseases are a major public health challenge,mainly affecting the elderly population and compromising their cognitive,sensory,and motor functions.Currently,available therapies focus on alleviating symptoms and slowing the progression of these conditions,but they do not yet offer a definitive cure.In this scenario,terpenes emerge as promising natural alternatives due to their neuroprotective properties.These compounds can reduce the formation of protein aggregates,neutralize free radicals,and inhibit pro-inflammatory enzymes,which are crucial factors in the development of neurodegenerative diseases.In addition,terpenes also play an important role in the regulation and remodeling of the extracellular matrix,a key target for improving neuronal functions.Substances such as linalool,pinene,and eugenol,among others,have potential therapeutic effects by modulating inflammatory and oxidative stress processes,the main factors that contribute to the progression of these diseases.Studies suggest that these compounds act on signaling pathways that regulate the extracellular matrix,improving neuronal integrity and,consequently,cognitive and motor function.This work aims to review the potential of terpenes in the treatment of neurodegenerative disorders,with emphasis on their ability to regulate oxidative stress and inflammation,as well as to remodel the extracellular matrix.The interaction between these mechanisms points to the promising use of terpenes as an innovative and natural therapeutic approach to combat these diseases.
基金supported by the National Key Research and Development Program(2022YFB3805800)National Natural Science Foundation of China(52473307,22208178,62301290)+9 种基金Taishan Scholar Program of Shandong Province in China(tsqn202211116)Shandong Provincial Universities Youth Innovation Technology Plan Team(2023KJ223)Natural Science Foundation of Shandong Province of China(ZR2023YQ037,ZR2020QE074,ZR2023QE043,ZR2022QE174)Shandong Province Science and Technology Small and Medium sized Enterprise Innovation Ability Enhancement Project(2023TSGC0344,2023TSGC1006)Natural Science Foundation of Qingdao(23-2-1-249-zyyd-jch,24-4-4-zrjj-56-jch)Anhui Province Postdoctoral Researcher Research Activity Funding Project(2023B706)Qingdao Key Technology Research and Industrialization Demonstration Projects(23-1-7-zdfn-2-hz)Qingdao Shinan District Science and Technology Plan Project(2022-3-005-DZ)Suqian Key Research and Development Plan(H202310)Jinan City-School Integration Development Strategy Project for the Year 2023 under Grant(JNSX2023088).
文摘Rehabilitation training is believed to be an effectual strategy that canreduce the risk of dysfunction caused by spasticity.However,achieving visualizationrehabilitation training for patients remains clinically challenging.Herein,wepropose visual rehabilitation training system including iontronic meta-fabrics withskin-friendly and large matrix features,as well as high-resolution image modules fordistribution of human muscle tension.Attributed to the dynamic connection and dissociationof the meta-fabric,the fabric exhibits outstanding tactile sensing properties,such as wide tactile sensing range(0~300 kPa)and high-resolution tactile perception(50 Pa or 0.058%).Meanwhile,thanks to the differential capillary effect,the meta-fabric exhibits a“hitting three birds with one stone”property(dryness wearing experience,long working time and cooling sensing).Based on this,the fabrics can be integrated with garmentsand advanced data analysis systems to manufacture a series of large matrix structure(40×40,1600 sensing units)training devices.Significantly,the tunability of piezo-ionic dynamics of the meta-fabric and the programmability of high-resolution imaging modules allowthis visualization training strategy extendable to various common disease monitoring.Therefore,we believe that our study overcomes theconstraint of standard spasticity rehabilitation training devices in terms of visual display and paves the way for future smart healthcare.
文摘This paper investigates the development and performance of a new higher-order geometric stiffness matrix that more closely approximates the theoretically derived stiffness coefficients.Factors that influence the accuracy of the solution are studied using two columns,two braced frames,and one unbraced frame.Discussion is provided when the new geometric stiffness matrix can be used to improve the buckling load analysis results and when it may provide only nominal additional benefit.
基金financially supported by grants from Royan In-stitute(grant No.400000200)Bahar Tashkhis Teb Co.(BTT,9703,9809,and 9903)。
文摘Background: Despite considerable advancements in identifying factors contributing to the development of hepatocellular carcinoma(HCC), the pathogenesis of HCC remains unclear. In many cases, HCC is a consequence of prolonged liver fibrosis, resulting in the formation of an intricate premalignant microenvironment. The accumulation of extracellular matrix(ECM) is a hallmark of premalignant microenvironment. Given the critical role of different matrix components in regulating cell phenotype and function, this study aimed to elucidate the interplay between the fibrotic matrix and malignant features in HCC. Methods: Liver tissues from both control(normal) and carbon tetrachloride(CCl_(4))-induced fibrotic rats were decellularized using sodium dodecyl sulfate(SDS) and Triton X-100. The resulting hydrogel from decellularized ECM was processed into micro-particles via the water-in-oil emulsion method. Microparticles were subsequently incorporated into three-dimensional liver biomimetic micro-tissues(MTs) comprising Huh-7 cells, human umbilical vein endothelial cells(HUVECs), and LX-2 cells. The MTs were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay at day 11, immunofluorescence staining, immunoblotting, and spheroid migration assay at day 14 after co-culture. Results: Fibrotic matrix from CCl4-treated rat livers significantly enhanced the growth rate of the MTs and their expression of CCND1 as compared to the normal one. Fibrotic matrix, also induced the expression of epithelial-to-mesenchymal transition(EMT)-associated genes such as TWIST1, ACTA2, MMP9, CDH2, and VIMENTIN in the MTs as compared to the normal matrix. Conversely, the expression of CDH1 and hepatic maturation genes HNF4A, ALB, CYP3A4 was decreased in the MTs when the fibrotic matrix was used. Furthermore, the fibrotic matrix increased the migration of the MTs and their secretion of alpha-fetoprotein. Conclusions: Our findings suggest a regulatory role for the fibrotic matrix in promoting cancerous phenotype, which could potentially accelerate the progression of malignancy in the liver.
基金supported by the National Natural Science Foundation of China(No.52101138,No.52201075)the Natural Science Foundation of Hubei Province(No.2023AFB798,No.2022CFB614)+3 种基金the Shenzhen Science and Technology Program(No.JCYJ20220530160813032)the State Key Laboratory of Solidification Processing in NWPU(No.SKLSP202309,No.SKLSP202308)the Guangdong Basic and Applied Basic Research Foundation(No.2022A1515011227)the State Key Laboratory of Powder Metallurgy of Central South University(No.SklpmKF-05)。
文摘B2-CuZr phase reinforced amorphous alloy matrix composites has become one of the research hotspots in the field of materials science due to the“transformation-induced plasticity”phenomenon,which makes the composites show better macroscopic plastic deformability and obvious work-hardening behavior compared to the conventional amorphous alloy matrix composites reinforced with ductile phases.However,the in-situ metastable B2-CuZr phase tends to undergo eutectoid decomposition during solidification,and the volume fraction,size,and distribution of B2-CuZr phase are difficult to control,which limits the development and application of these materials.To date,much efforts have been made to solve the above problems through composition optimization,casting parameter tailoring,and post-processing technique.In this study,a review was given based on relevant studies,focusing on the predictive approach,reinforcing mechanism,and microstructure tailoring methods of B2-CuZr phase reinforced amorphous alloy matrix composites.The research focus and future prospects were also given for the future development of the present composite system.
文摘BACKGROUND Demineralized bone matrix(DBM)is a commonly utilized allogenic bone graft substitute to promote osseous union.However,little is known regarding outcomes following DBM utilization in foot and ankle surgical procedures.AIM To evaluate the clinical and radiographic outcomes following DBM as a biological adjunct in foot and ankle surgical procedures.METHODS During May 2023,the PubMed,EMBASE and Cochrane library databases were systematically reviewed to identify clinical studies examining outcomes following DBM for the management of various foot and ankle pathologies.Data regarding study characteristics,patient demographics,subjective clinical outcomes,radiological outcomes,complications,and failure rates were extracted and analyzed.In addition,the level of evidence(LOE)and quality of evidence(QOE)for each individual study was also assessed.Thirteen studies were included in this review.RESULTS In total,363 patients(397 ankles and feet)received DBM as part of their surgical procedure at a weighted mean follow-up time of 20.8±9.2 months.The most common procedure performed was ankle arthrodesis in 94 patients(25.9%).Other procedures performed included hindfoot fusion,1st metatarsophalangeal joint arthrodesis,5th metatarsal intramedullary screw fixation,hallux valgus correction,osteochondral lesion of the talus repair and unicameral talar cyst resection.The osseous union rate in the ankle and hindfoot arthrodesis cohort,base of the 5th metatarsal cohort,and calcaneal fracture cohort was 85.6%,100%,and 100%,respectively.The weighted mean visual analog scale in the osteochondral lesions of the talus cohort improved from a pre-operative score of 7.6±0.1 to a post-operative score of 0.4±0.1.The overall complication rate was 27.2%,the most common of which was non-union(8.8%).There were 43 failures(10.8%)all of which warranted a further surgical procedure.CONCLUSION This current systematic review demonstrated that the utilization of DBM in foot and ankle surgical procedures led to satisfactory osseous union rates with favorable wound complication rates.Excellent outcomes were observed in patients undergoing fracture fixation augmented with DBM,with mixed evidence supporting the routine use of DBM in fusion procedures of the ankle and hindfoot.However,the low LOE together with the low QOE and significant heterogeneity between the included studies reinforces the need for randomized control trials to be conducted to identify the optimal role of DBM in the setting of foot and ankle surgical procedures.
基金Supported by National Natural Science Foundation of China(Grant No.51375437)Zhejiang Provincial Natural Science Foundation of China(Grant No.LY12E05019)
文摘The current research of configurable product disassemblability focuses on disassemblability evaluation and disassembly sequence planning. Little work has been done on quantitative analysis of configurable product disassemblability. The disassemblability modeling technology for configurable product based on disassembly constraint relation weighted design structure matrix （DSM） is proposed. Major factors affecting the disassemblability of configurable product are analyzed, and the disassembling degrees between components in configurable product are obtained by calculating disassembly entropies such as joint type, joint quantity, disassembly path, disassembly accessibility and material compatibility. The disassembly constraint relation weighted DSM of configurable product is constructed and configuration modules are formed by matrix decomposition and tearing operations. The disassembly constraint relation in configuration modules is strong coupling, and the disassembly constraint relation between modules is weak coupling, and the disassemblability configuration model is constructed based on configuration module. Finally, taking a hydraulic forging press as an example, the decomposed weak coupling components are used as configuration modules alone, components with a strong coupling are aggregated into configuration modules, and the disassembly sequence of components inside configuration modules is optimized by tearing operation. A disassemblability configuration model of the hydraulic forging press is constructed. By researching the disassemblability modeling technology of product configuration design based on disassembly constraint relation weighted DSM, the disassembly property in maintenance, recycling and reuse of configurable product are optimized.
