White Hypsizygus marmoreus is a popular edible mushroom.Its mycelium is easy to be contaminated by Penicillium,which leads to a decrease in its quality and yield.Penicillium could compete for limited space and nutrien...White Hypsizygus marmoreus is a popular edible mushroom.Its mycelium is easy to be contaminated by Penicillium,which leads to a decrease in its quality and yield.Penicillium could compete for limited space and nutrients through rapid growth and produce a variety of harmful gases,such as benzene,aldehydes,phenols,etc.,to inhibit the growth of H.marmoreus mycelium.A series of changes occurred in H.marmoreus proteome after contamination when detected by the label-free tandem mass spectrometry(MS/MS)technique.Some proteins with up-regulated expression worked together to participate in some processes,such as the non-toxic transformation of harmful gases,glutathione metabolism,histone modification,nucleotide excision repair,clearing misfolded proteins,and synthesizing glutamine,which were mainly used in response to biological stress.The proteins with down-regulated expression are mainly related to the processes of ribosome function,protein processing,spliceosome,carbon metabolism,glycolysis,and gluconeogenesis.The reduction in the function of these proteins affected the production of the cell components,which might be an adjustment to adapt to growth retardation.This study further enhanced the understanding of the biological stress response and the growth restriction adaptation mechanisms in edible fungi.It also provided a theoretical basis for protein function exploration and edible mushroom food safety research.展开更多
The self-made mobile fermentation bag for spent white Hypsizygus marmoreus substrate was developed, and the relevant fermentation process was studied. The results showed that under the condition of single addition of ...The self-made mobile fermentation bag for spent white Hypsizygus marmoreus substrate was developed, and the relevant fermentation process was studied. The results showed that under the condition of single addition of Lactobacillus,nitrogen-free extract was degraded into lactic acid, leading to decreased pH value.On day 10, the fermentation effect reached the best with Lactobacillus abundance of 5.12×10~7/ml, lactic acid content of 0.48% and strong acid flavor. At this time, the fermentation material was moist without mildew and agglomeration, and was suitable for livestock and poultry. However, after 10 days, undesirable acids and mildew generated, a large amount of lactic acid bacteria died, and the fermentation material turned black and agglomerated, and became unsuitable for feeding livestock and poultry. Throughout the fermentation process, the pH value first decreased continuously until to 4.0, and then remained stable. During the fermentation of spent white H. marmoreus substrate, the nitrogen-free extract and crude fiber contents decreased, the crude protein content increased, while other indicators remained unchanged.展开更多
Hypsizygus marmoreus has a significant dependence on environment at the mycelium growth stage. ln order to col ect, process and display real-time data of temperature, humidity, CO2 concentration in cultivation room, a...Hypsizygus marmoreus has a significant dependence on environment at the mycelium growth stage. ln order to col ect, process and display real-time data of temperature, humidity, CO2 concentration in cultivation room, along-distance environ-ment monitoring system with multi-technology is designed. The curves of tempera-ture, humidity and CO2 concentration, fitted by the MATLAB data fitting toolbox with the experimental data, reflected the changing trend of environmental factors. The mathematical model of CO2 concentration was established by regression analysis, and the regulation strategy that the ventilation period of half an hour and the venti-lation duration of 5 minutes was put forward.展开更多
Investigation on the cultures of Hypsizygus marmoreus resulted in the isolation of a new viscidane diterpene,8-oxoviscida-3,11(18)-diene-13,14,15,19-tetraol(1)and two new polyacetylenes,(E)-10-(1,1-dimethyl-2-propenyl...Investigation on the cultures of Hypsizygus marmoreus resulted in the isolation of a new viscidane diterpene,8-oxoviscida-3,11(18)-diene-13,14,15,19-tetraol(1)and two new polyacetylenes,(E)-10-(1,1-dimethyl-2-propenyloxy)-2-decene-4,6,8-triyn-1-ol(2)and 10-(1,1-dimethyl-2-propenyloxy)deca-4,6,8-triyn-1-ol(3),together with two known polyacetylenes,(E)-2-decen-4,6,8-triyn-1-ol(4)and 4,6,8-decatriyn-1-ol(5).Their structures were elucidated on the basis of extensive spectroscopic studies.Compound 1 is the first finding of viscidane diterpene in mushrooms.Compounds 1,3 and 5 were tested for cytotoxicity against human tumor cell lines HL-60,SMMC-7721,A-549,MCF-7 and SW-480.None of the compounds showed cytotoxic activity(IC50[40 lM).展开更多
Hypsizygus marmoreus is one of the most important edible fungi in Basidiomycete division and includes white and gray strains.However,very limited knowledge is known about the genomic structures and the genetic basis f...Hypsizygus marmoreus is one of the most important edible fungi in Basidiomycete division and includes white and gray strains.However,very limited knowledge is known about the genomic structures and the genetic basis for the white/gray diversity of this mushroom.Here,we report the near-complete high-quality H.marmoreus genome at the chromosomal level.Comparative genomics analysis indicates that chromosome structures were relatively conserved,and variations in collinearity and chromosome number were mainly attributed by chromosome split/fusion events in Aragicales,whereas the fungi genome experienced many genomic chromosome fracture,fusion,and genomic replication events after the split of Aragicales from Basidiomycetes.Resequencing of 57 strains allows us to classify the population into four major groups and associate genetic variations with morphological features,indicating that white strains were not originated independently.We further generated genetic populations and identified a cytochrome P450 as the candidate causal gene for the melanogenesis in H.marmoreus based on bulked segregant analysis (BSA)and comparative transcriptome analysis.The high-quality H.marmoreus genome and diversity data compiled in this study provide new knowledge and resources for the molecular breeding of H.marmoreus as well as the evolution of Basidiomycete.展开更多
研究了苎麻副产物含量、p H、含水量和添加剂等栽培基质对真姬菇栽培的影响,结果表明,培养基中苎麻副产物含量50%、水分含量65.0%、p H 6.0、并添加1%的碳酸钙和1%的白糖时,栽培真姬菇的效果较好,生物学效率达158%以上。与对照棉子壳栽...研究了苎麻副产物含量、p H、含水量和添加剂等栽培基质对真姬菇栽培的影响,结果表明,培养基中苎麻副产物含量50%、水分含量65.0%、p H 6.0、并添加1%的碳酸钙和1%的白糖时,栽培真姬菇的效果较好,生物学效率达158%以上。与对照棉子壳栽培效果差异不显著,表明苎麻副产物可以代替50%的棉子壳用来栽培真姬菇。展开更多
基金funded by the Shandong Provincial Natural Science Foundation,China (ZR2020QC005)the National Natural Science Foundation of China (32272789)+3 种基金the National Natural Science Foundation of China (32000041)the Shandong Edible Fungus Agricultural Technology System (SDAIT-07-02)the Shandong Provincial Key Research and Development Plan (2021ZDSYS28)the Qingdao Agricultural University Scientific Research Foundation (6631120076)。
文摘White Hypsizygus marmoreus is a popular edible mushroom.Its mycelium is easy to be contaminated by Penicillium,which leads to a decrease in its quality and yield.Penicillium could compete for limited space and nutrients through rapid growth and produce a variety of harmful gases,such as benzene,aldehydes,phenols,etc.,to inhibit the growth of H.marmoreus mycelium.A series of changes occurred in H.marmoreus proteome after contamination when detected by the label-free tandem mass spectrometry(MS/MS)technique.Some proteins with up-regulated expression worked together to participate in some processes,such as the non-toxic transformation of harmful gases,glutathione metabolism,histone modification,nucleotide excision repair,clearing misfolded proteins,and synthesizing glutamine,which were mainly used in response to biological stress.The proteins with down-regulated expression are mainly related to the processes of ribosome function,protein processing,spliceosome,carbon metabolism,glycolysis,and gluconeogenesis.The reduction in the function of these proteins affected the production of the cell components,which might be an adjustment to adapt to growth retardation.This study further enhanced the understanding of the biological stress response and the growth restriction adaptation mechanisms in edible fungi.It also provided a theoretical basis for protein function exploration and edible mushroom food safety research.
基金Supported by Education and Research Projects for Yong and Middle-aged Teachers in Fujian Province(JB13188)Longyan Science and Technology Plan Project(2014LY63,2015LY32)Production-study-research Cooperation Project of Longyan University(LC2014010)~~
文摘The self-made mobile fermentation bag for spent white Hypsizygus marmoreus substrate was developed, and the relevant fermentation process was studied. The results showed that under the condition of single addition of Lactobacillus,nitrogen-free extract was degraded into lactic acid, leading to decreased pH value.On day 10, the fermentation effect reached the best with Lactobacillus abundance of 5.12×10~7/ml, lactic acid content of 0.48% and strong acid flavor. At this time, the fermentation material was moist without mildew and agglomeration, and was suitable for livestock and poultry. However, after 10 days, undesirable acids and mildew generated, a large amount of lactic acid bacteria died, and the fermentation material turned black and agglomerated, and became unsuitable for feeding livestock and poultry. Throughout the fermentation process, the pH value first decreased continuously until to 4.0, and then remained stable. During the fermentation of spent white H. marmoreus substrate, the nitrogen-free extract and crude fiber contents decreased, the crude protein content increased, while other indicators remained unchanged.
基金Supported by National Natural Science Foundation of China(61263007)National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2013BDA16B04)~~
文摘Hypsizygus marmoreus has a significant dependence on environment at the mycelium growth stage. ln order to col ect, process and display real-time data of temperature, humidity, CO2 concentration in cultivation room, along-distance environ-ment monitoring system with multi-technology is designed. The curves of tempera-ture, humidity and CO2 concentration, fitted by the MATLAB data fitting toolbox with the experimental data, reflected the changing trend of environmental factors. The mathematical model of CO2 concentration was established by regression analysis, and the regulation strategy that the ventilation period of half an hour and the venti-lation duration of 5 minutes was put forward.
基金the National Natural Sciences Foundation of China(21302194).
文摘Investigation on the cultures of Hypsizygus marmoreus resulted in the isolation of a new viscidane diterpene,8-oxoviscida-3,11(18)-diene-13,14,15,19-tetraol(1)and two new polyacetylenes,(E)-10-(1,1-dimethyl-2-propenyloxy)-2-decene-4,6,8-triyn-1-ol(2)and 10-(1,1-dimethyl-2-propenyloxy)deca-4,6,8-triyn-1-ol(3),together with two known polyacetylenes,(E)-2-decen-4,6,8-triyn-1-ol(4)and 4,6,8-decatriyn-1-ol(5).Their structures were elucidated on the basis of extensive spectroscopic studies.Compound 1 is the first finding of viscidane diterpene in mushrooms.Compounds 1,3 and 5 were tested for cytotoxicity against human tumor cell lines HL-60,SMMC-7721,A-549,MCF-7 and SW-480.None of the compounds showed cytotoxic activity(IC50[40 lM).
基金supported by program for Seed Innovation and Industrialization in Fujian Province-Breeding and Industrialization of Major Edible Fungithe Science and Technology Major Project of Fujian Province (2016NZ0001)+1 种基金the Program for New Century Excellent Talents in Fujian Province (KLa17073A)agricultural technology extension service system for Edible fungus industry in Fujian, China (KNJ-153011-1)。
文摘Hypsizygus marmoreus is one of the most important edible fungi in Basidiomycete division and includes white and gray strains.However,very limited knowledge is known about the genomic structures and the genetic basis for the white/gray diversity of this mushroom.Here,we report the near-complete high-quality H.marmoreus genome at the chromosomal level.Comparative genomics analysis indicates that chromosome structures were relatively conserved,and variations in collinearity and chromosome number were mainly attributed by chromosome split/fusion events in Aragicales,whereas the fungi genome experienced many genomic chromosome fracture,fusion,and genomic replication events after the split of Aragicales from Basidiomycetes.Resequencing of 57 strains allows us to classify the population into four major groups and associate genetic variations with morphological features,indicating that white strains were not originated independently.We further generated genetic populations and identified a cytochrome P450 as the candidate causal gene for the melanogenesis in H.marmoreus based on bulked segregant analysis (BSA)and comparative transcriptome analysis.The high-quality H.marmoreus genome and diversity data compiled in this study provide new knowledge and resources for the molecular breeding of H.marmoreus as well as the evolution of Basidiomycete.
文摘研究了苎麻副产物含量、p H、含水量和添加剂等栽培基质对真姬菇栽培的影响,结果表明,培养基中苎麻副产物含量50%、水分含量65.0%、p H 6.0、并添加1%的碳酸钙和1%的白糖时,栽培真姬菇的效果较好,生物学效率达158%以上。与对照棉子壳栽培效果差异不显著,表明苎麻副产物可以代替50%的棉子壳用来栽培真姬菇。
