Objective:To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus lancifolius.Methods:The various hydroethanolic extracts of Conocarpus lancifolius...Objective:To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus lancifolius.Methods:The various hydroethanolic extracts of Conocarpus lancifolius leaf were prepared by ultrasonication assisted freezedrying.Total phenolic contents,flavonoid contents,antioxidant activity,α-glucosidase andα-amylase inhibitions of leaf extracts were determined.The metabolite profiling was accomplished by UHPLC-Q-TOF-MS/MS analysis.The antidiabetic assessment of the most potent extract was carried out by measuring the hypoglycemic and hypolipidemic effect in the high fat diet-fed diabetic albino mice.The blood glucose level,haemoglobin,total cholesterol,high-density lipoproteins(HDL)and low-density lipoproteins(LDL)were determined.Results:The 60%ethanolic extract exhibited the highest phenolic and flavonoid contents of(349.39±2.13)mg GAE/g dry extract and(116.95±2.34)mg RE/g dry extracts,respectively,and the highest DPPH scavenging activity with an IC50 value of(32.87±1.11)μg/mL.The IC50 values forα-glucosidase andα-amylase inhibitions were(38.64±0.93)μg/mL and(44.80±1.57)μg/mL,respectively.UHPLC-Q-TOF-MS/MS analysis confirmed the presence of gallic acid,ellagic acid,corilagin,kaempherol-3-O-rutinoside,caffeic acid derivative,isorhamnetin and galloyl derivatives in the 60%ethanolic extract.Plant extract at a dose of 450 mg/kg body weight reduced blood glucose level,total cholesterol,LDL and HDL,and increased haemoglobin in alloxan-induced diabetic mice,Conclusions:Conocarpus lancifolius leaves are proved as a good source of biologically functional metabolites and possess antidiabetic activity which may be further explored to treat diabetes.展开更多
Aqueous leaf extract and pulverized leaf tissue were tested for allelopathic effects on seed germination, seedling growth traits and net photosynthesis of corn (Zea mays L.) and bean (Vigna sinensis L.) The chemical c...Aqueous leaf extract and pulverized leaf tissue were tested for allelopathic effects on seed germination, seedling growth traits and net photosynthesis of corn (Zea mays L.) and bean (Vigna sinensis L.) The chemical constituents of the leaf extract were determined by GC-MS and the efficacy of the leaf extract to control some soil-borne fungal pathogens was also evaluated. The leaf extract inhibited germination, radicle and plumule lengths, root and shoot growth, chlorophyll content, photosynthesis and nutrient uptake in both crops. In corn and bean plants growth inhibition was proportional to the concentrations of the extracts (2.5% - 7.5%). Seed germination was associated with water uptake and the solute potential of the extracts. Leaf extracts also reduced of chlorophyll content, and stressed photosynthetic function by reducing photochemical efficiency (Fv/Fm) and electron transport rate (ETR) of PS II in corn and bean plants. Corn was more tolerant to the leaf extract than bean plants. The GC-MS analysis detected thirty one and thirty three compounds in winter and summer leaf extracts, respectively. The majority was phenolic compounds. Mycelia growth of Fusarium oxysporum f. sp. lycopersicon, Sclerotinia sclerotiorum, and Rhizoctonia solani was inhibited and in 10% extract the fungi died. The extract however, had no effect on Trichoderma harzianum. Leaf extract of C. lancifolius exhibited both herbicidal and fungicidal properties but cannot be recommended for biological control of soil borne fungal plant pathogens because it has the potential to physiologically stress or damage cultivated plants or crops.展开更多
文摘Objective:To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus lancifolius.Methods:The various hydroethanolic extracts of Conocarpus lancifolius leaf were prepared by ultrasonication assisted freezedrying.Total phenolic contents,flavonoid contents,antioxidant activity,α-glucosidase andα-amylase inhibitions of leaf extracts were determined.The metabolite profiling was accomplished by UHPLC-Q-TOF-MS/MS analysis.The antidiabetic assessment of the most potent extract was carried out by measuring the hypoglycemic and hypolipidemic effect in the high fat diet-fed diabetic albino mice.The blood glucose level,haemoglobin,total cholesterol,high-density lipoproteins(HDL)and low-density lipoproteins(LDL)were determined.Results:The 60%ethanolic extract exhibited the highest phenolic and flavonoid contents of(349.39±2.13)mg GAE/g dry extract and(116.95±2.34)mg RE/g dry extracts,respectively,and the highest DPPH scavenging activity with an IC50 value of(32.87±1.11)μg/mL.The IC50 values forα-glucosidase andα-amylase inhibitions were(38.64±0.93)μg/mL and(44.80±1.57)μg/mL,respectively.UHPLC-Q-TOF-MS/MS analysis confirmed the presence of gallic acid,ellagic acid,corilagin,kaempherol-3-O-rutinoside,caffeic acid derivative,isorhamnetin and galloyl derivatives in the 60%ethanolic extract.Plant extract at a dose of 450 mg/kg body weight reduced blood glucose level,total cholesterol,LDL and HDL,and increased haemoglobin in alloxan-induced diabetic mice,Conclusions:Conocarpus lancifolius leaves are proved as a good source of biologically functional metabolites and possess antidiabetic activity which may be further explored to treat diabetes.
文摘Aqueous leaf extract and pulverized leaf tissue were tested for allelopathic effects on seed germination, seedling growth traits and net photosynthesis of corn (Zea mays L.) and bean (Vigna sinensis L.) The chemical constituents of the leaf extract were determined by GC-MS and the efficacy of the leaf extract to control some soil-borne fungal pathogens was also evaluated. The leaf extract inhibited germination, radicle and plumule lengths, root and shoot growth, chlorophyll content, photosynthesis and nutrient uptake in both crops. In corn and bean plants growth inhibition was proportional to the concentrations of the extracts (2.5% - 7.5%). Seed germination was associated with water uptake and the solute potential of the extracts. Leaf extracts also reduced of chlorophyll content, and stressed photosynthetic function by reducing photochemical efficiency (Fv/Fm) and electron transport rate (ETR) of PS II in corn and bean plants. Corn was more tolerant to the leaf extract than bean plants. The GC-MS analysis detected thirty one and thirty three compounds in winter and summer leaf extracts, respectively. The majority was phenolic compounds. Mycelia growth of Fusarium oxysporum f. sp. lycopersicon, Sclerotinia sclerotiorum, and Rhizoctonia solani was inhibited and in 10% extract the fungi died. The extract however, had no effect on Trichoderma harzianum. Leaf extract of C. lancifolius exhibited both herbicidal and fungicidal properties but cannot be recommended for biological control of soil borne fungal plant pathogens because it has the potential to physiologically stress or damage cultivated plants or crops.
