The pivotal role of N^(6)-methyladenosine(m^(6)A)demethylases in regulating plant stress responses has been widely explored;however,the function of apple m^(6)A demethylases under heat stress and fixed-carbon starvati...The pivotal role of N^(6)-methyladenosine(m^(6)A)demethylases in regulating plant stress responses has been widely explored;however,the function of apple m^(6)A demethylases under heat stress and fixed-carbon starvation is unclear.In this study,the apple RNA demethylase gene family was identified,and the demethylase gene MdALKBH1A was selected for further analysis.Using liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis,we demonstrated that MdALKBH1A is the m^(6)A demethylase of apple.Moreover,transgenic‘Micro Tom’tomato plants overexpressing MdALKBH1A were more sensitive to high temperature,probably due to the decreased antioxidant ability,increased membrane lipid peroxidation and reduced plasma membrane stability.However,these tomato plants overexpressing MdALKBH1A were more resistant to fixed-carbon starvation,as evidenced by the improved plasma membrane stability,enhanced photosynthetic rates and elevated autophagic activity.In summary,our results highlight the crucial role played by Md ALKBH1A in the response of apple plants to high-temperature stress and fixedcarbon starvation.展开更多
基金supported by the earmarked fund for the China Agricultural Research System(CARS-27)。
文摘The pivotal role of N^(6)-methyladenosine(m^(6)A)demethylases in regulating plant stress responses has been widely explored;however,the function of apple m^(6)A demethylases under heat stress and fixed-carbon starvation is unclear.In this study,the apple RNA demethylase gene family was identified,and the demethylase gene MdALKBH1A was selected for further analysis.Using liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis,we demonstrated that MdALKBH1A is the m^(6)A demethylase of apple.Moreover,transgenic‘Micro Tom’tomato plants overexpressing MdALKBH1A were more sensitive to high temperature,probably due to the decreased antioxidant ability,increased membrane lipid peroxidation and reduced plasma membrane stability.However,these tomato plants overexpressing MdALKBH1A were more resistant to fixed-carbon starvation,as evidenced by the improved plasma membrane stability,enhanced photosynthetic rates and elevated autophagic activity.In summary,our results highlight the crucial role played by Md ALKBH1A in the response of apple plants to high-temperature stress and fixedcarbon starvation.
