Effects of nitrogen(N) and phosphorus(P) from different sources and at different concentrations on the growth of Levanderina fissa(= Gyrodinium instriatum) were studied in laboratory conditions. The findings might exp...Effects of nitrogen(N) and phosphorus(P) from different sources and at different concentrations on the growth of Levanderina fissa(= Gyrodinium instriatum) were studied in laboratory conditions. The findings might explain the recurrent blooms of this species in Pearl River Estuary, China. Results showed that nutrient limitation significantly inhibited the growth of L. fissa. The values of specific growth rate(μmax) and half-saturation nutrient concentration(KS) were 0.37 divisions/d and 8.49 μmol L^(-1) for N, and 0.39 divisions/d and 1.99 μmol L^(-1) for P, respectively. Based on KS values, dissolved inorganic N level in PRE was sufficient to support the high proliferation of L. fissa, while dissolved inorganic P concentration was far lower than the minimum requirement for its effective growth. L. fissa was not able to utilize dissolved organic N(DON) compounds such as urea, amino acids, and uric acid. However, it grew well by using a wide variety of dissolved organic P(DOP) sources like nucleotides, glycerophosphate, and 4-nitrophenylphosphate. The results from this study suggested that the ability in DOP utilization of L. fissa offers this species a competitive advantage in phytoplankton communities. The high level and continuous supply of DIN, enrichment of DOP, together with warm climate and low salinity in the Pearl River Estuary provided a suitable nutrient niche for the growth of L. fissa, and resulted in the recurrent blooms in the estuary.展开更多
This paper studies the growth law of 23-year-old Castanopsis fissa planta- tion in Haiming forest farm （Pingguo County, Baise City, Guangxi Province） with the method of stem analysis. The results show that the avera...This paper studies the growth law of 23-year-old Castanopsis fissa planta- tion in Haiming forest farm （Pingguo County, Baise City, Guangxi Province） with the method of stem analysis. The results show that the average DBH, tree height and volume of the 23-year-old C. fissa plantation are 19.4 cm, 20.4 m and 0.326 1 m3 respectively. The fast growing periods of DBH, tree height and volume are the 2^nd- 8^th, 6^th-11^th, 6^th-23^nd year. Annual increment curve and average increment curve of volume haven＇t intersected in the 23 years, which means the 23-year-old C. fissais plantation has not reached the quantitative mature age. The growth equations of DBH, tree height and volume are D=1.713 3T-0.050 6T2＋0.000 7T2-1.942 2, H= 1.925 7T-0.039 8T2-2.489 0, V=-0.010 4T-0.002 4T2-4.084 8e^-0.005 T2＋0.012 7. Ac- cording to the volume regression equation of Castanopsis fissa, it will reach quanti- tative maturity in the 24~ year.展开更多
[Objectives] To study the optimal extraction process for polysaccharides of Urtica fissa E. Pritz. [Methods] Leaves of wild U. fissa E. Pritz. collected in Nyingchi of Tibet were used as raw materials. Water extractio...[Objectives] To study the optimal extraction process for polysaccharides of Urtica fissa E. Pritz. [Methods] Leaves of wild U. fissa E. Pritz. collected in Nyingchi of Tibet were used as raw materials. Water extraction method was used to extract polysaccharides from U. fissa E. Pritz. Besides,comparison was made for the effects of extraction time,extraction temperature and solid-to-liquid ratio on the yield rate of polysaccharides from U. fissa E. Pritz. In addition,using the extraction time,extraction temperature and solid-to-liquid ratio as factors,L9( 33) orthogonal experiment was carried out to determine the optimal process parameters for water extraction of polysaccharides from U. fissa E. Pritz.[Results] The optimal process parameters: hot water extraction time was 4 h,the extraction temperature was 90℃,and the mass ratio of water to leaves of U. fissa E. Pritz. was 1∶ 25. Under such extraction conditions,the yield rate of polysaccharides from U. fissa E. Pritz.was up to 11. 611%. [Conclusions]This experiment is expected to provide certain reference for reasonable development and utilization of wild U. fissa E. Pritz. resources in Tibet.展开更多
Growth interactions between the dinoflagellate Levanderina fissa (=Gyrodinium instriatum) and the other three algal bloom species Skeletonema costatum (Bacillariophyceae), Prorocentrum micans (Dinophyceae), and Chatto...Growth interactions between the dinoflagellate Levanderina fissa (=Gyrodinium instriatum) and the other three algal bloom species Skeletonema costatum (Bacillariophyceae), Prorocentrum micans (Dinophyceae), and Chattonella marina (Raphido- phyceae) were studied using bi- or tri-algal co-cultures under laboratory conditions. The Lotka-Volterra model was applied to esti- mate the interspecific competition between them. Results showed that the growth of L. fissa was significantly suppressed in both bi-algal and tri-algal cultures, and the maximum cell densities in co-cultures were only between 21% and 69% of that in mono-cul- ture. L. fissa outcompeted P. micans;however, its effects on S. costatum and C. marina were generally less than the effects of their intraspecific competitions based on the competition coefficients. Contrarily, the inhibitory effects of S. costatum and C. marina on L. fissa were much greater. S. costatum outcompeted the other three species in all co-cultures, and its competition coefficients on the other species were more than 10000 times higher than the opposite. The growth of C. marina was suppressed by S. costatum;how- ever, it outcompeted L. fissa and P. micans. The growth of P. micans was most strongly inhibited in co-cultures, and the maximum cell densities in co-cultures were less than 15% of that in mono-species culture. The results suggested that L. fissa had few advan- tages in the interspecific competition. The adaptation to low salinity and the utilization of organic phosphorus might be the important reasons for L. fissa to take the competitive predominance in brackish estuarine environments.展开更多
In the present study, the BPH inhibitory activities of Urtica fissa were systematically investigated. Firstly, inhibitory activities of 5α reductase of the alcoholic extracts from different parts(root, stem, leaf, fl...In the present study, the BPH inhibitory activities of Urtica fissa were systematically investigated. Firstly, inhibitory activities of 5α reductase of the alcoholic extracts from different parts(root, stem, leaf, flower and fruit) were evaluated. The results indicated that the root possessed the most significant action. Subsequently, U. fissa root(UFR) was subjected to further pharmacological evaluation using the benign prostate hyperplasia(BPH) model rats induced by testosterone propionate. The results revealed that UFR could significantly decrease the prostate index, alter the hyperplasia tissue morphology, suppress the prostatic growth factors of VEGF, EGF, bF GF and KGF, decrease the inflammation factor levels of TNF-α, IL-1β and IL-6, improve the activities of GSH-Px, CAT and SOD and decrease the MDA level in the prostate of the model rats. Moreover, UFR also significantly suppressed the hormone levels of testosterone and dihydrotestosterone. These results indicated that the possible BPH inhibitory mechanisms of UFR were growth factor suppression, hormone level modulation, anti-inflammation, and anti-oxidative stress.展开更多
Objective:To investigate the analgesic substances in the aerial part of Urtica fissa(Urticae Fissae Herba),commonly used for rheumatoid and rheumatism arthritis.Methods:The analgesic constituents were isolated with th...Objective:To investigate the analgesic substances in the aerial part of Urtica fissa(Urticae Fissae Herba),commonly used for rheumatoid and rheumatism arthritis.Methods:The analgesic constituents were isolated with the active guidance of hot plate and acetic acid writhing models,and identified by comprehensive spectroscopic analysis.Results:Thirteen alkaloids(1–13),two lignans(14,15),and three amides(16–18)were isolated from the active fractions.Among them,compound 1 was a new alkaloid,and compound 6 was a new natural product.The activity evaluation in vivo indicated that various pyrrole alkaloids(1,3,6,and 12)possessed significant analgesic activities,they could significantly inhibit the mice pain response induced by acetic acid and hot plate at the dosage of 2 mg/kg BW.Conclusion:The study revealed that the pyrrole alkaloids played important roles in the analgesic activities of Urticae Fissae Herba.展开更多
目前全球性的氮沉降和施磷肥不断增加对森林土壤产生重要影响。藜蒴(Castanopsis fissa)是我国南方重要的用材树种之一,研究外源性氮和磷对藜蒴林土壤养分和生化特性的影响,可以为藜蒴人工林的可持续经营提供理论指导。针对广东省云勇...目前全球性的氮沉降和施磷肥不断增加对森林土壤产生重要影响。藜蒴(Castanopsis fissa)是我国南方重要的用材树种之一,研究外源性氮和磷对藜蒴林土壤养分和生化特性的影响,可以为藜蒴人工林的可持续经营提供理论指导。针对广东省云勇林场一片立地条件相似的14年生黎蒴人工林进行为期2年的模拟氮和磷沉降,设置不施肥(对照)、施N肥、施P肥、施N+P肥4种处理,对应的氮和磷施用量分别为0,200 kg N,80 kg P and200 kg N+80 kg P(hm-2·a-1),每种处理有8个重复。结果表明,加N减少了土壤全P、全K和速效K含量,增加了土壤碱解N含量;加P提高了土壤有机质、全N、全P、速效P和速效K含量,减少了土壤全K含量;加N+P提高了土壤有机质、全N、全P、碱解N和速效P含量,减少了土壤全K含量。加N、加P和加N+P处理增加了土壤细菌、真菌数量及磷酸酶和过氧化氢酶活性。加P处理还增加了土壤放线菌数量及土壤脲酶活性,加N+P处理也提高了脲酶活性。展开更多
树木之间的竞争关系是塑造森林结构和动态的关键因素。采用Hegyi单木竞争指数,定量分析了青秀山次生林优势种大叶栎Castanopsis fi ssa种内与种间的竞争关系。结果表明:1)大叶栎种内竞争强度随着胸径的增大而逐渐减小,这与种内的密度制...树木之间的竞争关系是塑造森林结构和动态的关键因素。采用Hegyi单木竞争指数,定量分析了青秀山次生林优势种大叶栎Castanopsis fi ssa种内与种间的竞争关系。结果表明:1)大叶栎种内竞争强度随着胸径的增大而逐渐减小,这与种内的密度制约性的自疏效应有关。2)大叶栎与乔木上层建群种马尾松Pinus massoniana的竞争最为强烈,其次为大叶栎、稠木Lithocarpus glabra、阴香Cinnamomum burmannii等树种。大叶栎种间竞争较种内竞争更为剧烈,竞争的结果可能会导致马尾松种群逐渐退出群落。3)大叶栎种内以及与整个林分的竞争强度与对象木胸径之间近似服从幂函数关系,而大叶栎与伴生树种的竞争强度与其胸径间近似服从对数函数关系,竞争强度和对象木胸径间呈显著的负相关关系。大叶栎种内和种间的竞争强度随大叶栎树龄的增大而减小,当其胸径超过30 cm后,种内和种间竞争强度的变化很小。展开更多
黧蒴锥是华南用于人工造林和生态恢复的优良乡土树种.选取浇水频率和凋落物覆盖量两个控制因子,研究在不同的土壤湿度和凋落物覆盖量条件下,黧蒴锥种子萌发及幼苗存活和生长特征.结果表明:凋落物对黧蒴锥种子萌发和幼苗生长的影响与环...黧蒴锥是华南用于人工造林和生态恢复的优良乡土树种.选取浇水频率和凋落物覆盖量两个控制因子,研究在不同的土壤湿度和凋落物覆盖量条件下,黧蒴锥种子萌发及幼苗存活和生长特征.结果表明:凋落物对黧蒴锥种子萌发和幼苗生长的影响与环境的潮湿程度相关.当浇水频率为每天1次时,凋落物覆盖会抑制黧蒴锥种子萌发和幼苗早期生长,导致种子萌发率降低、幼苗死亡率提高;在浇水频率为2 d 1次和3 d 1次时,凋落物覆盖可以改善土壤的水分状况,有助于种子萌发和幼苗存活.凋落物覆盖还可以显著地提高幼苗的生物量.在通过播种来实现亚热带灌木林地更新时,可利用凋落物覆盖促进黧蒴锥的种子萌发和幼苗存活.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.41276154,41476132)
文摘Effects of nitrogen(N) and phosphorus(P) from different sources and at different concentrations on the growth of Levanderina fissa(= Gyrodinium instriatum) were studied in laboratory conditions. The findings might explain the recurrent blooms of this species in Pearl River Estuary, China. Results showed that nutrient limitation significantly inhibited the growth of L. fissa. The values of specific growth rate(μmax) and half-saturation nutrient concentration(KS) were 0.37 divisions/d and 8.49 μmol L^(-1) for N, and 0.39 divisions/d and 1.99 μmol L^(-1) for P, respectively. Based on KS values, dissolved inorganic N level in PRE was sufficient to support the high proliferation of L. fissa, while dissolved inorganic P concentration was far lower than the minimum requirement for its effective growth. L. fissa was not able to utilize dissolved organic N(DON) compounds such as urea, amino acids, and uric acid. However, it grew well by using a wide variety of dissolved organic P(DOP) sources like nucleotides, glycerophosphate, and 4-nitrophenylphosphate. The results from this study suggested that the ability in DOP utilization of L. fissa offers this species a competitive advantage in phytoplankton communities. The high level and continuous supply of DIN, enrichment of DOP, together with warm climate and low salinity in the Pearl River Estuary provided a suitable nutrient niche for the growth of L. fissa, and resulted in the recurrent blooms in the estuary.
基金Supported by Guangxi Forestry Science and Technology Project~~
文摘This paper studies the growth law of 23-year-old Castanopsis fissa planta- tion in Haiming forest farm （Pingguo County, Baise City, Guangxi Province） with the method of stem analysis. The results show that the average DBH, tree height and volume of the 23-year-old C. fissa plantation are 19.4 cm, 20.4 m and 0.326 1 m3 respectively. The fast growing periods of DBH, tree height and volume are the 2^nd- 8^th, 6^th-11^th, 6^th-23^nd year. Annual increment curve and average increment curve of volume haven＇t intersected in the 23 years, which means the 23-year-old C. fissais plantation has not reached the quantitative mature age. The growth equations of DBH, tree height and volume are D=1.713 3T-0.050 6T2＋0.000 7T2-1.942 2, H= 1.925 7T-0.039 8T2-2.489 0, V=-0.010 4T-0.002 4T2-4.084 8e^-0.005 T2＋0.012 7. Ac- cording to the volume regression equation of Castanopsis fissa, it will reach quanti- tative maturity in the 24~ year.
基金Supported by Project of Natural Science Foundation of Tibet Autonomous Region(2016ZR-15-31)
文摘[Objectives] To study the optimal extraction process for polysaccharides of Urtica fissa E. Pritz. [Methods] Leaves of wild U. fissa E. Pritz. collected in Nyingchi of Tibet were used as raw materials. Water extraction method was used to extract polysaccharides from U. fissa E. Pritz. Besides,comparison was made for the effects of extraction time,extraction temperature and solid-to-liquid ratio on the yield rate of polysaccharides from U. fissa E. Pritz. In addition,using the extraction time,extraction temperature and solid-to-liquid ratio as factors,L9( 33) orthogonal experiment was carried out to determine the optimal process parameters for water extraction of polysaccharides from U. fissa E. Pritz.[Results] The optimal process parameters: hot water extraction time was 4 h,the extraction temperature was 90℃,and the mass ratio of water to leaves of U. fissa E. Pritz. was 1∶ 25. Under such extraction conditions,the yield rate of polysaccharides from U. fissa E. Pritz.was up to 11. 611%. [Conclusions]This experiment is expected to provide certain reference for reasonable development and utilization of wild U. fissa E. Pritz. resources in Tibet.
基金financially supported by the National Natural Science Foundation of China (No. 41476132)
文摘Growth interactions between the dinoflagellate Levanderina fissa (=Gyrodinium instriatum) and the other three algal bloom species Skeletonema costatum (Bacillariophyceae), Prorocentrum micans (Dinophyceae), and Chattonella marina (Raphido- phyceae) were studied using bi- or tri-algal co-cultures under laboratory conditions. The Lotka-Volterra model was applied to esti- mate the interspecific competition between them. Results showed that the growth of L. fissa was significantly suppressed in both bi-algal and tri-algal cultures, and the maximum cell densities in co-cultures were only between 21% and 69% of that in mono-cul- ture. L. fissa outcompeted P. micans;however, its effects on S. costatum and C. marina were generally less than the effects of their intraspecific competitions based on the competition coefficients. Contrarily, the inhibitory effects of S. costatum and C. marina on L. fissa were much greater. S. costatum outcompeted the other three species in all co-cultures, and its competition coefficients on the other species were more than 10000 times higher than the opposite. The growth of C. marina was suppressed by S. costatum;how- ever, it outcompeted L. fissa and P. micans. The growth of P. micans was most strongly inhibited in co-cultures, and the maximum cell densities in co-cultures were less than 15% of that in mono-species culture. The results suggested that L. fissa had few advan- tages in the interspecific competition. The adaptation to low salinity and the utilization of organic phosphorus might be the important reasons for L. fissa to take the competitive predominance in brackish estuarine environments.
基金National Natural Science Fund of China(Grant No.81374067)Shanghai Municipal Health Commission(Grant No.2018ZY002)College Students’Innovation Project(Grant No.IPP20216).
文摘In the present study, the BPH inhibitory activities of Urtica fissa were systematically investigated. Firstly, inhibitory activities of 5α reductase of the alcoholic extracts from different parts(root, stem, leaf, flower and fruit) were evaluated. The results indicated that the root possessed the most significant action. Subsequently, U. fissa root(UFR) was subjected to further pharmacological evaluation using the benign prostate hyperplasia(BPH) model rats induced by testosterone propionate. The results revealed that UFR could significantly decrease the prostate index, alter the hyperplasia tissue morphology, suppress the prostatic growth factors of VEGF, EGF, bF GF and KGF, decrease the inflammation factor levels of TNF-α, IL-1β and IL-6, improve the activities of GSH-Px, CAT and SOD and decrease the MDA level in the prostate of the model rats. Moreover, UFR also significantly suppressed the hormone levels of testosterone and dihydrotestosterone. These results indicated that the possible BPH inhibitory mechanisms of UFR were growth factor suppression, hormone level modulation, anti-inflammation, and anti-oxidative stress.
基金financially supported by a grant of National Natural Science Foundation of China (No.81374067)Shanghai Municipal Natural Science Foundation (No.20ZR1427700)Shanghai Municipal Health Commission (No.2018ZY002)
文摘Objective:To investigate the analgesic substances in the aerial part of Urtica fissa(Urticae Fissae Herba),commonly used for rheumatoid and rheumatism arthritis.Methods:The analgesic constituents were isolated with the active guidance of hot plate and acetic acid writhing models,and identified by comprehensive spectroscopic analysis.Results:Thirteen alkaloids(1–13),two lignans(14,15),and three amides(16–18)were isolated from the active fractions.Among them,compound 1 was a new alkaloid,and compound 6 was a new natural product.The activity evaluation in vivo indicated that various pyrrole alkaloids(1,3,6,and 12)possessed significant analgesic activities,they could significantly inhibit the mice pain response induced by acetic acid and hot plate at the dosage of 2 mg/kg BW.Conclusion:The study revealed that the pyrrole alkaloids played important roles in the analgesic activities of Urticae Fissae Herba.
文摘目前全球性的氮沉降和施磷肥不断增加对森林土壤产生重要影响。藜蒴(Castanopsis fissa)是我国南方重要的用材树种之一,研究外源性氮和磷对藜蒴林土壤养分和生化特性的影响,可以为藜蒴人工林的可持续经营提供理论指导。针对广东省云勇林场一片立地条件相似的14年生黎蒴人工林进行为期2年的模拟氮和磷沉降,设置不施肥(对照)、施N肥、施P肥、施N+P肥4种处理,对应的氮和磷施用量分别为0,200 kg N,80 kg P and200 kg N+80 kg P(hm-2·a-1),每种处理有8个重复。结果表明,加N减少了土壤全P、全K和速效K含量,增加了土壤碱解N含量;加P提高了土壤有机质、全N、全P、速效P和速效K含量,减少了土壤全K含量;加N+P提高了土壤有机质、全N、全P、碱解N和速效P含量,减少了土壤全K含量。加N、加P和加N+P处理增加了土壤细菌、真菌数量及磷酸酶和过氧化氢酶活性。加P处理还增加了土壤放线菌数量及土壤脲酶活性,加N+P处理也提高了脲酶活性。
文摘黧蒴锥是华南用于人工造林和生态恢复的优良乡土树种.选取浇水频率和凋落物覆盖量两个控制因子,研究在不同的土壤湿度和凋落物覆盖量条件下,黧蒴锥种子萌发及幼苗存活和生长特征.结果表明:凋落物对黧蒴锥种子萌发和幼苗生长的影响与环境的潮湿程度相关.当浇水频率为每天1次时,凋落物覆盖会抑制黧蒴锥种子萌发和幼苗早期生长,导致种子萌发率降低、幼苗死亡率提高;在浇水频率为2 d 1次和3 d 1次时,凋落物覆盖可以改善土壤的水分状况,有助于种子萌发和幼苗存活.凋落物覆盖还可以显著地提高幼苗的生物量.在通过播种来实现亚热带灌木林地更新时,可利用凋落物覆盖促进黧蒴锥的种子萌发和幼苗存活.
