Objective: To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebr...Objective: To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebrae (L3) transverse process syndrome model rat and to observe the effect of needle-knife therapy. Methods: One hundred and sixty male SD rats were randomly divided into normal group, model group, aminoguanidine (AG) group, needle-knife group, 40 rats in each group. The L3 transverse process syndrome rat model was established, and after treatment of needle-knife and AG, iNOS activities and NO contents and histomorpholocal changes in the soft tissues around L3 transverse process on 1, 3, 7 and 14 days were observed in the groups. Results: Compared with the normal group, iNOS activity and NO content in the model group were significantly increased (P<0.01); Compared with the model group, iNOS activities and NO contents were significantly decreased in both the needle-knife group and the AG group (both P<0.01); And both iNOS activities and NO contents were identical with both local inflammation response and injured degrees of the injured tissue in the groups. Conclusion: Needle-knife therapy can significantly inhibit generation of NO, alleviate inflammatory response and injured degree of the injured soft tissue, improve microcirculation, prevent formation of pathological scar tissue, and promote repair of the chronic soft tissue injury.展开更多
目的利用文献计量学和可视化方法,总结和分析运动诱导的氧化应激在骨骼肌健康和功能障碍领域的已有研究,预测前沿趋势。方法基于Web of Science检索2003年1月1日至2022年12月31日的文献。使用Scimago Graphica、CiteSpace和VOSviewer对...目的利用文献计量学和可视化方法,总结和分析运动诱导的氧化应激在骨骼肌健康和功能障碍领域的已有研究,预测前沿趋势。方法基于Web of Science检索2003年1月1日至2022年12月31日的文献。使用Scimago Graphica、CiteSpace和VOSviewer对排名靠前的国家、机构、作者、关键词进行分析。结果共纳入文献4185篇,文章数量总体呈快速增长趋势。美国、巴西和中国的研究成果居于领先地位。Radak Z.和Powers S.K.分别是表文章和被引用次数最多的作者。主要关键词为“体力活动”“自由基”“脂质过氧化”。关键词突现的分析表明,以“肌肉减少症”和“肌肉减少性肥胖症”为代表的老年慢性病的分子机制,可能成为运动诱导氧化应激作用于骨骼肌健康和功能障碍领域的未来研究方向。结论本研究对骨骼肌健康和功能障碍中运动诱导的氧化应激进行了文献计量分析,有助于了解该领域的整体发展情况,并为该领域的研究人员提供了多方面的见解。展开更多
基金supported by a grant from National "973" Project (No: 2006CB504508)
文摘Objective: To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebrae (L3) transverse process syndrome model rat and to observe the effect of needle-knife therapy. Methods: One hundred and sixty male SD rats were randomly divided into normal group, model group, aminoguanidine (AG) group, needle-knife group, 40 rats in each group. The L3 transverse process syndrome rat model was established, and after treatment of needle-knife and AG, iNOS activities and NO contents and histomorpholocal changes in the soft tissues around L3 transverse process on 1, 3, 7 and 14 days were observed in the groups. Results: Compared with the normal group, iNOS activity and NO content in the model group were significantly increased (P<0.01); Compared with the model group, iNOS activities and NO contents were significantly decreased in both the needle-knife group and the AG group (both P<0.01); And both iNOS activities and NO contents were identical with both local inflammation response and injured degrees of the injured tissue in the groups. Conclusion: Needle-knife therapy can significantly inhibit generation of NO, alleviate inflammatory response and injured degree of the injured soft tissue, improve microcirculation, prevent formation of pathological scar tissue, and promote repair of the chronic soft tissue injury.
