It is essential to determine the occurrence of emerging organic contaminants(EOCs),such as pharmaceuticals and personal care products(PPCPs),in the ambient environment to address growing public concerns.However,such a...It is essential to determine the occurrence of emerging organic contaminants(EOCs),such as pharmaceuticals and personal care products(PPCPs),in the ambient environment to address growing public concerns.However,such analysis is quite challenging due to the low trace level of such contaminants in water,which therefore requires several litres of water samples.In this study,a large volume solid phase extraction(LV-SPE)device was developed and evaluated for its performance in monitoring PPCPs as an example.Relatively good recoveries and reproducibility were obtained under specific operating conditions:a water sample volume of no more than 20 L,a flow rate not exceeding 120 mL/min,and a methanol elution volume of at least 30 mL.In addition,the results from the onsite enrichment approach using LV-SPE were compared with those from the conventional approach using a standard SPE device in the laboratory for real groundwater samples.Among the eight selected PPCPs(nalidixic acid,carbamazepine,bezafibrate,clofibric acid,sulfadiazine,sulfamethoxypyridazine,sulfamethazine and sulfamonomethoxine),LV-SPE approach detected more target compounds.While the detected concentrations were generally comparable,slightly higher concentrations were observed for carbamazepine,clofibric acid,sulfadiazine and sulfamethazine using the LV-SPE method.The developed LV-SPE device provides an alternative approach for trace analysis of PPCPs and may also be applicable for other emerging organic contaminants.展开更多
The analysis of endogenous glycoproteins and glycopeptides in human body fluids is of great importance for screening and discovering disease biomarkers with clinical significance.However,the presence of interfering su...The analysis of endogenous glycoproteins and glycopeptides in human body fluids is of great importance for screening and discovering disease biomarkers with clinical significance.However,the presence of interfering substances makes the direct quantitative detection of low-abundance glycoproteins and glycopeptides in human body fluids one of the great challenges in analytical chemistry.Magnetic solid phase extraction(MSPE)has the advantages of easy preparation,low cost and good magnetic responsiveness.Magnetic adsorbents are the core of MSPE technology,and magnetic adsorbents based on different functional materials are widely used in the quantitative analysis of glycoproteins and glycopeptides in human body fluids,making it possible to analyze glycoproteins and glycopeptides with low abundance as well as multiple types,which provides a technical platform for screening and evaluating glycoproteins and glycopeptides in body fluids as disease biomarkers.In this paper,we focus on the recent advances in the application of MSPE technology and magnetic adsorbents for the separation and enrichment of glycoproteins and glycopeptides in human body fluids,and the future trends and application prospects in this field are also presented.展开更多
A lab scale membrane bioreactor system was built to investigate the removal of Di-2-Ethyl Hexyl Phthalates (DEHP) in wastewater under variation of three runs: two hydraulic retention time (HRT) 24 and 36 hours in addi...A lab scale membrane bioreactor system was built to investigate the removal of Di-2-Ethyl Hexyl Phthalates (DEHP) in wastewater under variation of three runs: two hydraulic retention time (HRT) 24 and 36 hours in addition to two biomass: concentrated and light sludge. Solid phase extraction (SPE) followed by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was applied to quantitatively identify DEHP in wastewater samples. Membrane bioreactor was built and operated to investigate DEHP removal. Higher HRT removed DEHP more efficiently than lower HRT. The concentrated MLSS could achieve higher removal efficiency than the lighter MLSS at the same HRT value. The performance of MBR in removing DEHP, TOC and COD from wastewater with a maximum removal efficiency were 29%, 85%, and 98%, respectively. Mass balance of DEHP in the system indicated that a majority was removed by adsorption process rather than filtration or microbiological process.展开更多
A new method of using phenolphthalein loaded with 1-(2-pyridylazo)-2-naphthol (PAN) as solid phase extractant to separate and enrich trace copper in diverse water samples has been developed. The ef- fects of different...A new method of using phenolphthalein loaded with 1-(2-pyridylazo)-2-naphthol (PAN) as solid phase extractant to separate and enrich trace copper in diverse water samples has been developed. The ef- fects of different parameters, such as the amounts of PAN and phenolphthalein, stirring time, various salts and metal ions on the enrichment yield of Cu(II) have been investigated to select the experimental conditions. Under the optimum conditions, Cu(II) can be totally adsorbed on the surface of microcrys- talline phenolphthalein. By controlling appropriate acidity (pH=1.0), Cu(II) is completely separated from Cd(II), Pb(II), Mn(II), Co(II), Fe(III), Ni(II), Al(III), Zn(II) and Hg(II) in the solution. The possible reaction mechanism of the enrichment of copper is discussed in detail in this paper. The detection limit of this proposed method is found to be 0.03 μg·L-1 with the enrichment factor of 200. The recovery is in a range of 96.8%―105%. The standard deviation is less than 2.5%. The proposed method has been successfully applied to the determination of trace copper in different water samples with satisfactory results.展开更多
基于膜式固相萃取法和液相色谱-串联质谱法建立了一种高灵敏、快速分析环境水样中痕量全氟化合物(PFASs)的方法。采用最优条件的模式固相萃取进行样品前处理,以乙酸铵水溶液及乙腈为流动相进行梯度洗脱,经Accucore aQ C18色谱柱分离,采...基于膜式固相萃取法和液相色谱-串联质谱法建立了一种高灵敏、快速分析环境水样中痕量全氟化合物(PFASs)的方法。采用最优条件的模式固相萃取进行样品前处理,以乙酸铵水溶液及乙腈为流动相进行梯度洗脱,经Accucore aQ C18色谱柱分离,采用电喷雾离子源、负离子采集模式和质谱多反应监测模式分析,实现了环境水样中17种全氟化合物的快速分析,17种全氟化合物检出限(S/N=3)为0.2~0.3 ng/L。将方法应用于实际环境水样中全氟化合物的分析,加标回收率为87%~95%。研究可为快速、有效地检测环境水样中痕量全氟化合物提供新方法的选择。展开更多
基金This research was financially supported by the Major Science and Technology Program for Water Pollution Control and Treatment in China(Grant No.2017ZX07202004).
文摘It is essential to determine the occurrence of emerging organic contaminants(EOCs),such as pharmaceuticals and personal care products(PPCPs),in the ambient environment to address growing public concerns.However,such analysis is quite challenging due to the low trace level of such contaminants in water,which therefore requires several litres of water samples.In this study,a large volume solid phase extraction(LV-SPE)device was developed and evaluated for its performance in monitoring PPCPs as an example.Relatively good recoveries and reproducibility were obtained under specific operating conditions:a water sample volume of no more than 20 L,a flow rate not exceeding 120 mL/min,and a methanol elution volume of at least 30 mL.In addition,the results from the onsite enrichment approach using LV-SPE were compared with those from the conventional approach using a standard SPE device in the laboratory for real groundwater samples.Among the eight selected PPCPs(nalidixic acid,carbamazepine,bezafibrate,clofibric acid,sulfadiazine,sulfamethoxypyridazine,sulfamethazine and sulfamonomethoxine),LV-SPE approach detected more target compounds.While the detected concentrations were generally comparable,slightly higher concentrations were observed for carbamazepine,clofibric acid,sulfadiazine and sulfamethazine using the LV-SPE method.The developed LV-SPE device provides an alternative approach for trace analysis of PPCPs and may also be applicable for other emerging organic contaminants.
基金supported by the Natural Science Foundation of Jilin Provincial Science&Technology Department(No.20190201079JC)。
文摘The analysis of endogenous glycoproteins and glycopeptides in human body fluids is of great importance for screening and discovering disease biomarkers with clinical significance.However,the presence of interfering substances makes the direct quantitative detection of low-abundance glycoproteins and glycopeptides in human body fluids one of the great challenges in analytical chemistry.Magnetic solid phase extraction(MSPE)has the advantages of easy preparation,low cost and good magnetic responsiveness.Magnetic adsorbents are the core of MSPE technology,and magnetic adsorbents based on different functional materials are widely used in the quantitative analysis of glycoproteins and glycopeptides in human body fluids,making it possible to analyze glycoproteins and glycopeptides with low abundance as well as multiple types,which provides a technical platform for screening and evaluating glycoproteins and glycopeptides in body fluids as disease biomarkers.In this paper,we focus on the recent advances in the application of MSPE technology and magnetic adsorbents for the separation and enrichment of glycoproteins and glycopeptides in human body fluids,and the future trends and application prospects in this field are also presented.
文摘A lab scale membrane bioreactor system was built to investigate the removal of Di-2-Ethyl Hexyl Phthalates (DEHP) in wastewater under variation of three runs: two hydraulic retention time (HRT) 24 and 36 hours in addition to two biomass: concentrated and light sludge. Solid phase extraction (SPE) followed by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was applied to quantitatively identify DEHP in wastewater samples. Membrane bioreactor was built and operated to investigate DEHP removal. Higher HRT removed DEHP more efficiently than lower HRT. The concentrated MLSS could achieve higher removal efficiency than the lighter MLSS at the same HRT value. The performance of MBR in removing DEHP, TOC and COD from wastewater with a maximum removal efficiency were 29%, 85%, and 98%, respectively. Mass balance of DEHP in the system indicated that a majority was removed by adsorption process rather than filtration or microbiological process.
基金Supported by the Natural Science Foundation of Henan Province (Grant No. 0511020500) Innovation Project Foundation of University in Henan Province (Grant No. [2001]513)
文摘A new method of using phenolphthalein loaded with 1-(2-pyridylazo)-2-naphthol (PAN) as solid phase extractant to separate and enrich trace copper in diverse water samples has been developed. The ef- fects of different parameters, such as the amounts of PAN and phenolphthalein, stirring time, various salts and metal ions on the enrichment yield of Cu(II) have been investigated to select the experimental conditions. Under the optimum conditions, Cu(II) can be totally adsorbed on the surface of microcrys- talline phenolphthalein. By controlling appropriate acidity (pH=1.0), Cu(II) is completely separated from Cd(II), Pb(II), Mn(II), Co(II), Fe(III), Ni(II), Al(III), Zn(II) and Hg(II) in the solution. The possible reaction mechanism of the enrichment of copper is discussed in detail in this paper. The detection limit of this proposed method is found to be 0.03 μg·L-1 with the enrichment factor of 200. The recovery is in a range of 96.8%―105%. The standard deviation is less than 2.5%. The proposed method has been successfully applied to the determination of trace copper in different water samples with satisfactory results.
文摘基于膜式固相萃取法和液相色谱-串联质谱法建立了一种高灵敏、快速分析环境水样中痕量全氟化合物(PFASs)的方法。采用最优条件的模式固相萃取进行样品前处理,以乙酸铵水溶液及乙腈为流动相进行梯度洗脱,经Accucore aQ C18色谱柱分离,采用电喷雾离子源、负离子采集模式和质谱多反应监测模式分析,实现了环境水样中17种全氟化合物的快速分析,17种全氟化合物检出限(S/N=3)为0.2~0.3 ng/L。将方法应用于实际环境水样中全氟化合物的分析,加标回收率为87%~95%。研究可为快速、有效地检测环境水样中痕量全氟化合物提供新方法的选择。
