Background Research on low-protein-level diets has indicated that even though the profiles of essential amino acids(EAAs)follow the recommendation for a normal-protein-level diet,broilers fed low-protein diets failed ...Background Research on low-protein-level diets has indicated that even though the profiles of essential amino acids(EAAs)follow the recommendation for a normal-protein-level diet,broilers fed low-protein diets failed to achieve pro-ductive performance compared to those fed normal diets.Therefore,it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance.Furthermore,identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology.In this study,12 dietary treatments[Con(+),Con(-),L&A(-),L&A(+),M&C(-),M&C(+),BCAA(-),BCAA(+),Thr(-),Thr(+),Trp(-)and Trp(+)]were established by combining different EAAs including lysine and argi-nine,methionine and cysteine,branched-chain amino acid(BCAA),threonine,and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets.Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments,biomarkers associated with opti-mal and suboptimal amino acid balance were identified.Results Growth performance,carcass characteristics,hepatic enzyme activity,serum biochemical parameters,and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns.Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers.For the low-protein diet,the dietary concentrations of lysine,arginine,and tryptophan,but not of methionine,cystine,or threonine,needed to be increased.Therefore,further research on individual BCAA is required.For untar-geted metabolomic analysis,Con(+)was selected as a normal diet(NP)while Con(-)represented a low-protein diet(LP).L&A(+)denotes a low-protein amino acid balanced diet(LPAB)and Thr(+)represents a low-protein amino acid imbalance diet(LPAI).The metabolites oxypurinol,pantothenic acid,and D-octopine in birds were significantly influ-enced by different dietary amino acid patterns.Conclusion Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets.Oxypurinol,pantothenic acid,and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.展开更多
Promoting agricultural production and ensuring the supply of agricultural products has always been the main task of agricultural development in China,but the agricultural production in China has not yet been combined ...Promoting agricultural production and ensuring the supply of agricultural products has always been the main task of agricultural development in China,but the agricultural production in China has not yet been combined with the Chinese residents' dietary needs to formulate reasonable development goals,with a certain blindness in production. According to the dietary standards in The Dietary Guidelines for Chinese Residents developed by the Chinese Nutrition Society,we calculate the demand of various types of agricultural products in China under the conditions of balanced diet. In comparison with the output of various types of agricultural products in China at present,we find that the output of most of agricultural products in China has exceeded the reasonable demand of the Chinese residents under the conditions of balanced diet. Therefore,adjusting the agricultural production structure in China and advocating balanced diet has become an important way to solve the problem of balance between supply and demand of agricultural products.展开更多
To examine whether lanthanum carbonate can induce a low phosphorus status in experimental animals, we examined phosphorus balance in rats administered lanthanum carbonate. Male 8-week-old Wistar rats were fed a basal ...To examine whether lanthanum carbonate can induce a low phosphorus status in experimental animals, we examined phosphorus balance in rats administered lanthanum carbonate. Male 8-week-old Wistar rats were fed a basal case-in-based semi-purified diet or the basal diet supplemented with 0.45% or 0.90% lanthanum as lanthanum carbonate for 4 weeks. Lanthanum administration did not influence body or several organ weights and liver function. In rats administered lanthanum, a very small quantity of lanthanum was detected in several organs although the apparent absorption was almost zero. The highest lanthanum concentration was observed in the liver followed by the femur and kidney. Lanthanum was not clearly detected in the brain. Differences in organ lanthanum between 0.45% and 0.90% administration groups were not significant;lanthanum accumulation in the body is very low and may reach a plateau in a certain range of intake. Serum phosphorus was decreased and fecal phosphorus was increased by lanthanum administration dose-dependently. Urinary phosphorus excretion was significantly decreased by lanthanum. Since urinary phosphorus of rats fed 0.45% lanthanum diet decreased to near zero, the highest phosphorus balance was observed in rats fed 0.45% lanthanum diet. This high balance is considered to be adaptation to the low phosphorus status induced by lanthanum carbonate. These results indicate that lanthanum carbonate can induce hypophosphatemia without any direct side effects and be used to examine the effect of removing phosphorus from the diet in animal nutritional studies.展开更多
目的分析均衡营养素膳食联合二甲双胍治疗对多囊卵巢综合征患者糖脂代谢的影响。方法选取2021年10月至2023年10月聊城市第二人民医院收治的100例多囊卵巢综合征患者作为研究对象进行回顾性分析,按治疗方案的不同将其分为对照组(n=50)和...目的分析均衡营养素膳食联合二甲双胍治疗对多囊卵巢综合征患者糖脂代谢的影响。方法选取2021年10月至2023年10月聊城市第二人民医院收治的100例多囊卵巢综合征患者作为研究对象进行回顾性分析,按治疗方案的不同将其分为对照组(n=50)和研究组(n=50)。对照组采用二甲双胍治疗,研究组采用均衡营养素膳食联合二甲双胍治疗。比较两组患者治疗前后的血糖、血脂、性激素、胰岛素抵抗指标水平。结果研究组的糖化血红蛋白(HbA1c)、空腹血糖(FBG)、餐后2 h血糖(2 h PG)水平低于对照组,差异有统计学意义(P<0.05)。研究组的总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)水平低于对照组,高密度脂蛋白胆固醇(HDL-C)水平高于对照组,差异有统计学意义(P<0.05)。研究组的黄体生成素(LH)水平低于对照组,卵泡刺激素(FSH)、雌二醇(E2)水平高于对照组,差异有统计学意义(P<0.05)。研究组的空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)低于对照组,胰岛β细胞功能指数(HOMA-β)高于对照组,差异有统计学意义(P<0.05)。结论均衡营养素膳食联合二甲双胍治疗用于多囊卵巢综合征患者,可改善其糖脂代谢、性激素水平,效果显著。展开更多
Background:The study objective was to test the hypothesis that 1)lowering dietary crude protein(CP)increases dietary energetic efficiency and reduces metabolic heat associated with lactation,and 2)excessive dietary le...Background:The study objective was to test the hypothesis that 1)lowering dietary crude protein(CP)increases dietary energetic efficiency and reduces metabolic heat associated with lactation,and 2)excessive dietary leucine(Leu)supplementation in a low CP diet decreases dietary energetic efficiency and increases metabolic heat associated with lactation.Methods:Fifty-four lactating multiparous Yorkshire sows were allotted to 1 of 3 isocaloric diets(10.80 MJ/kg net energy):1)control(CON;18.75%CP),2)reduced CP with a near ideal or optimal AA profile(OPT;13.75%CP)and 3)diet OPT with excessive Leu(OPTLEU;14.25%CP).Sow body weight and backfat were recorded on day 1 and 21 of lactation and piglets were weighed on day 1,4,8,14,18,and 21 of lactation.Energy balance was measured on sows during early(day 4 to 8)and peak(day 14 to18)lactation,and milk was sampled on day 8 and 18.Results:Over 21-day lactation,sows fed OPT lost body weight and body lipid(P<0.05).In peak lactation,sows fed OPT had higher milk energy output(P<0.05)than CON.Sows fed OPTLEU tended(P=0.07)to have less milk energy output than OPT and did not differ from CON.Maternal energy retention was lower(P<0.05)in OPT and OPTLEU compared to CON sows,and did not differ between OPTLEU and OPT sows.Sows fed OPT had higher(P<0.05)apparent energy efficiency for milk production compared to CON.Heat production associated with lactation was lower(P<0.05)or tended to be lower(P=0.082),respectively,in OPT and OPTLEU compared to CON sows.Conclusion:The OPT diet,in peak lactation,improved dietary energy utilization for lactation due to less urinary energy and metabolic heat loss,and triggered dietary energy deposition into milk at the expense of maternal lipid mobilization.Leucine supplementation above requirement may reduce dietary energy utilization for lactation by decreasing the energy partitioning towards milk,partially explaining the effectiveness of OPT diet over CON diets.展开更多
基金Shenyang Governmental Science and Technology Program(Project No.22-316-2-02)China Agriculture Research System Program(Project No.CARS-41-G04).
文摘Background Research on low-protein-level diets has indicated that even though the profiles of essential amino acids(EAAs)follow the recommendation for a normal-protein-level diet,broilers fed low-protein diets failed to achieve pro-ductive performance compared to those fed normal diets.Therefore,it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance.Furthermore,identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology.In this study,12 dietary treatments[Con(+),Con(-),L&A(-),L&A(+),M&C(-),M&C(+),BCAA(-),BCAA(+),Thr(-),Thr(+),Trp(-)and Trp(+)]were established by combining different EAAs including lysine and argi-nine,methionine and cysteine,branched-chain amino acid(BCAA),threonine,and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets.Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments,biomarkers associated with opti-mal and suboptimal amino acid balance were identified.Results Growth performance,carcass characteristics,hepatic enzyme activity,serum biochemical parameters,and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns.Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers.For the low-protein diet,the dietary concentrations of lysine,arginine,and tryptophan,but not of methionine,cystine,or threonine,needed to be increased.Therefore,further research on individual BCAA is required.For untar-geted metabolomic analysis,Con(+)was selected as a normal diet(NP)while Con(-)represented a low-protein diet(LP).L&A(+)denotes a low-protein amino acid balanced diet(LPAB)and Thr(+)represents a low-protein amino acid imbalance diet(LPAI).The metabolites oxypurinol,pantothenic acid,and D-octopine in birds were significantly influ-enced by different dietary amino acid patterns.Conclusion Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets.Oxypurinol,pantothenic acid,and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.
基金Supported by the Special Fund of Chinese Central Government for Basic Scientific Research Operations in Public-interest Research Institutes (202-23)
文摘Promoting agricultural production and ensuring the supply of agricultural products has always been the main task of agricultural development in China,but the agricultural production in China has not yet been combined with the Chinese residents' dietary needs to formulate reasonable development goals,with a certain blindness in production. According to the dietary standards in The Dietary Guidelines for Chinese Residents developed by the Chinese Nutrition Society,we calculate the demand of various types of agricultural products in China under the conditions of balanced diet. In comparison with the output of various types of agricultural products in China at present,we find that the output of most of agricultural products in China has exceeded the reasonable demand of the Chinese residents under the conditions of balanced diet. Therefore,adjusting the agricultural production structure in China and advocating balanced diet has become an important way to solve the problem of balance between supply and demand of agricultural products.
文摘To examine whether lanthanum carbonate can induce a low phosphorus status in experimental animals, we examined phosphorus balance in rats administered lanthanum carbonate. Male 8-week-old Wistar rats were fed a basal case-in-based semi-purified diet or the basal diet supplemented with 0.45% or 0.90% lanthanum as lanthanum carbonate for 4 weeks. Lanthanum administration did not influence body or several organ weights and liver function. In rats administered lanthanum, a very small quantity of lanthanum was detected in several organs although the apparent absorption was almost zero. The highest lanthanum concentration was observed in the liver followed by the femur and kidney. Lanthanum was not clearly detected in the brain. Differences in organ lanthanum between 0.45% and 0.90% administration groups were not significant;lanthanum accumulation in the body is very low and may reach a plateau in a certain range of intake. Serum phosphorus was decreased and fecal phosphorus was increased by lanthanum administration dose-dependently. Urinary phosphorus excretion was significantly decreased by lanthanum. Since urinary phosphorus of rats fed 0.45% lanthanum diet decreased to near zero, the highest phosphorus balance was observed in rats fed 0.45% lanthanum diet. This high balance is considered to be adaptation to the low phosphorus status induced by lanthanum carbonate. These results indicate that lanthanum carbonate can induce hypophosphatemia without any direct side effects and be used to examine the effect of removing phosphorus from the diet in animal nutritional studies.
文摘目的分析均衡营养素膳食联合二甲双胍治疗对多囊卵巢综合征患者糖脂代谢的影响。方法选取2021年10月至2023年10月聊城市第二人民医院收治的100例多囊卵巢综合征患者作为研究对象进行回顾性分析,按治疗方案的不同将其分为对照组(n=50)和研究组(n=50)。对照组采用二甲双胍治疗,研究组采用均衡营养素膳食联合二甲双胍治疗。比较两组患者治疗前后的血糖、血脂、性激素、胰岛素抵抗指标水平。结果研究组的糖化血红蛋白(HbA1c)、空腹血糖(FBG)、餐后2 h血糖(2 h PG)水平低于对照组,差异有统计学意义(P<0.05)。研究组的总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)水平低于对照组,高密度脂蛋白胆固醇(HDL-C)水平高于对照组,差异有统计学意义(P<0.05)。研究组的黄体生成素(LH)水平低于对照组,卵泡刺激素(FSH)、雌二醇(E2)水平高于对照组,差异有统计学意义(P<0.05)。研究组的空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)低于对照组,胰岛β细胞功能指数(HOMA-β)高于对照组,差异有统计学意义(P<0.05)。结论均衡营养素膳食联合二甲双胍治疗用于多囊卵巢综合征患者,可改善其糖脂代谢、性激素水平,效果显著。
基金This study was financially supported by USDA-NIFA(award number 2014–67015-21832).
文摘Background:The study objective was to test the hypothesis that 1)lowering dietary crude protein(CP)increases dietary energetic efficiency and reduces metabolic heat associated with lactation,and 2)excessive dietary leucine(Leu)supplementation in a low CP diet decreases dietary energetic efficiency and increases metabolic heat associated with lactation.Methods:Fifty-four lactating multiparous Yorkshire sows were allotted to 1 of 3 isocaloric diets(10.80 MJ/kg net energy):1)control(CON;18.75%CP),2)reduced CP with a near ideal or optimal AA profile(OPT;13.75%CP)and 3)diet OPT with excessive Leu(OPTLEU;14.25%CP).Sow body weight and backfat were recorded on day 1 and 21 of lactation and piglets were weighed on day 1,4,8,14,18,and 21 of lactation.Energy balance was measured on sows during early(day 4 to 8)and peak(day 14 to18)lactation,and milk was sampled on day 8 and 18.Results:Over 21-day lactation,sows fed OPT lost body weight and body lipid(P<0.05).In peak lactation,sows fed OPT had higher milk energy output(P<0.05)than CON.Sows fed OPTLEU tended(P=0.07)to have less milk energy output than OPT and did not differ from CON.Maternal energy retention was lower(P<0.05)in OPT and OPTLEU compared to CON sows,and did not differ between OPTLEU and OPT sows.Sows fed OPT had higher(P<0.05)apparent energy efficiency for milk production compared to CON.Heat production associated with lactation was lower(P<0.05)or tended to be lower(P=0.082),respectively,in OPT and OPTLEU compared to CON sows.Conclusion:The OPT diet,in peak lactation,improved dietary energy utilization for lactation due to less urinary energy and metabolic heat loss,and triggered dietary energy deposition into milk at the expense of maternal lipid mobilization.Leucine supplementation above requirement may reduce dietary energy utilization for lactation by decreasing the energy partitioning towards milk,partially explaining the effectiveness of OPT diet over CON diets.
