Objective:To explore the expression of hsa_circ_0046701 in patients with glioma and its correlation with clinicopathological features and prognosis of glioma,and to study its clinical value.Methods:qRT-PCR was used to...Objective:To explore the expression of hsa_circ_0046701 in patients with glioma and its correlation with clinicopathological features and prognosis of glioma,and to study its clinical value.Methods:qRT-PCR was used to detect the expression of hsa_circ_0046701 in glioma tissues and normal brain tissues,and chi-square test was used to analyze the effects of different levels of hsa_circ_0046701 expression on the clinicopathological features of patients.The survival curve was drawn by Kaplan-Meier method,and the univariate and multivariate analysis of prognosis was performed by Cox proportional hazard regression model.Results:The expression level of hsa_circ_0046701 in gliomas was significantly higher than that in normal brain tissues,and the expression in gradeⅢ-Ⅳgliomas was higher than that gradeⅠ-Ⅱgliomas in WHO grade.The expression of hsa_circ_0046701 was significantly correlated with WHO grade,KPS score and postoperative recurrence,but not with age,sex,tumor size,number and location.The five-year survival rate of the group with high expression of hsa_circ_0046701 was lower than that of the group with low expression of hsa_circ_0046701.The patients with high expression of hsa_circ_0046701,had high WHO grade and the KPS score was less than 80.Postoperative recurrence was an independent prognostic factor for glioma patients.Conclusion:Hsa_circ_0046701 is highly expressed in gliomas,which is positively correlated with the malignant degree of gliomas,and can be used as a potential biological marker.High expression of hsa_circ_0046701,high WHO grade,KPS score<80,postoperative recurrence are the key factors affecting the prognosis of patients,which can be used as independent prognostic factors for patients.展开更多
AIM:To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts(HPFs)and its correlation with the severity grades of pterygium.METHODS:Pterygium and normal conjuncti...AIM:To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts(HPFs)and its correlation with the severity grades of pterygium.METHODS:Pterygium and normal conjunctival tissues were collected from the superior area of the same patient’s eye(n=33).The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reversetranscription polymerase chain reaction(RT-qPCR)were analyzed.Three distinct siRNA sequences targeting hsa_circ_0007482,along with a negative control sequence,were transfected into HPFs.Cell proliferation was assessed using the cell counting kit-8.Expression levels of Ki67,proliferating cell nuclear antigen(PCNA),Cyclin D1,Bax,B-cell lymphoma-2(Bcl-2),and Caspase-3 were measured via RT-qPCR.Immunofluorescence staining was employed to detect Ki67 and vimentin expressions.Apoptosis was evaluated using flow cytometry.RESULTS:Hsa_circ_0007482 expression was significantly higher in pterygium tissues compared to normal conjunctival tissues(P<0.001).Positive correlations were observed between hsa_circ_0007482 expression and pterygium severity,thickness,and vascular density.Knockdown of hsa_circ_0007482 inhibited cell proliferation,reducing the mRNA expression of Ki67,PCNA,and Cyclin D1 in HPFs.Hsa_circ_0007482 knockdown induced apoptosis,increasing mRNA expression levels of Bax and Caspase-3,while decreasing Bcl-2 expression in HPFs.Additionally,hsa_circ_0007482 knockdown attenuated vimentin expression in HPFs.CONCLUSION:The downregulation of hsa_circ_0007482 effectively hampers cell proliferation and triggers apoptosis in HPFs.There are discernible positive correlations detected between the expression of hsa_circ_0007482 and the severity of pterygium.展开更多
基金National Natural Science Foundation of China(No.81901188)。
文摘Objective:To explore the expression of hsa_circ_0046701 in patients with glioma and its correlation with clinicopathological features and prognosis of glioma,and to study its clinical value.Methods:qRT-PCR was used to detect the expression of hsa_circ_0046701 in glioma tissues and normal brain tissues,and chi-square test was used to analyze the effects of different levels of hsa_circ_0046701 expression on the clinicopathological features of patients.The survival curve was drawn by Kaplan-Meier method,and the univariate and multivariate analysis of prognosis was performed by Cox proportional hazard regression model.Results:The expression level of hsa_circ_0046701 in gliomas was significantly higher than that in normal brain tissues,and the expression in gradeⅢ-Ⅳgliomas was higher than that gradeⅠ-Ⅱgliomas in WHO grade.The expression of hsa_circ_0046701 was significantly correlated with WHO grade,KPS score and postoperative recurrence,but not with age,sex,tumor size,number and location.The five-year survival rate of the group with high expression of hsa_circ_0046701 was lower than that of the group with low expression of hsa_circ_0046701.The patients with high expression of hsa_circ_0046701,had high WHO grade and the KPS score was less than 80.Postoperative recurrence was an independent prognostic factor for glioma patients.Conclusion:Hsa_circ_0046701 is highly expressed in gliomas,which is positively correlated with the malignant degree of gliomas,and can be used as a potential biological marker.High expression of hsa_circ_0046701,high WHO grade,KPS score<80,postoperative recurrence are the key factors affecting the prognosis of patients,which can be used as independent prognostic factors for patients.
基金Supported by Guangdong Basic and Applied Basic Research Foundation (No.2021A1515111012).
文摘AIM:To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts(HPFs)and its correlation with the severity grades of pterygium.METHODS:Pterygium and normal conjunctival tissues were collected from the superior area of the same patient’s eye(n=33).The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reversetranscription polymerase chain reaction(RT-qPCR)were analyzed.Three distinct siRNA sequences targeting hsa_circ_0007482,along with a negative control sequence,were transfected into HPFs.Cell proliferation was assessed using the cell counting kit-8.Expression levels of Ki67,proliferating cell nuclear antigen(PCNA),Cyclin D1,Bax,B-cell lymphoma-2(Bcl-2),and Caspase-3 were measured via RT-qPCR.Immunofluorescence staining was employed to detect Ki67 and vimentin expressions.Apoptosis was evaluated using flow cytometry.RESULTS:Hsa_circ_0007482 expression was significantly higher in pterygium tissues compared to normal conjunctival tissues(P<0.001).Positive correlations were observed between hsa_circ_0007482 expression and pterygium severity,thickness,and vascular density.Knockdown of hsa_circ_0007482 inhibited cell proliferation,reducing the mRNA expression of Ki67,PCNA,and Cyclin D1 in HPFs.Hsa_circ_0007482 knockdown induced apoptosis,increasing mRNA expression levels of Bax and Caspase-3,while decreasing Bcl-2 expression in HPFs.Additionally,hsa_circ_0007482 knockdown attenuated vimentin expression in HPFs.CONCLUSION:The downregulation of hsa_circ_0007482 effectively hampers cell proliferation and triggers apoptosis in HPFs.There are discernible positive correlations detected between the expression of hsa_circ_0007482 and the severity of pterygium.
