Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size ...Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.展开更多
Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows： Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were domin...Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows： Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were dominated by cortex rooting, accounting for the 77% of clones. There were 5 clones which occurred with both cal us rooting and cortex rooting during rooting process. The rooting efficiency index （REI） showed a positive correlation with the rooting rate. Under natural tem-perature condition, keeping moisture and shading, we found that the highest survival rate occurred in early June, September, and October.展开更多
Populus species are important resources for industry and in scientific study on biological and agricul- tural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar （Populus cil...Populus species are important resources for industry and in scientific study on biological and agricul- tural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar （Populus ciliata wall. ex Royle）. The effect of TDZ alone and in combi- nation with adenine and NAA was studied on the regen- eration potential of petiole explants. The explants were excised from Himalayan poplar plants grown in glass- houses. After surface sterilization the explants were cul- tured on shoot induction medium. High percentage shoot regeneration （86 %） was recorded on MS medium sup- plemented with 0.004 mg L-1 TDZ and 79.7 mg L-1 adenine. The regenerated shoots for elongation and multi- plication were transferred to MS ＋ 0.5 mg L-1 BAP ＋ 0.2 mg L-1 IAA ＋ 0.3 mg L-1 GA3. Root re- generation from shoots developed in vitro was observed on MS medium supplemented with 0.10 mg L-1 IBA. Hi- malayan poplar plantlets could be produced within 2 months after acclimatization in a sterile mixture of sand and soil. We developed a high efficiency plant regeneration protocol from petiole explants of P. ciliata.展开更多
To investigate the population dynamics of Toona ciliata in Qizimeishan Na- ture Reserve, the age classes and population quantity of Toona clliata in Qizimeishan were analyzed by transforming diameter classes into corr...To investigate the population dynamics of Toona ciliata in Qizimeishan Na- ture Reserve, the age classes and population quantity of Toona clliata in Qizimeishan were analyzed by transforming diameter classes into corresponding age classes. A static life table was prepared. Moreover, the curves of survival rate, mortality rate and killing powder were drawn so as to perform spectral analysis for Toona clliata in Qizimeishan Nature Reserve. The results showed that the Vpi and Vpf of the Toona ci/iata population were 32.32% and 4.039%, respectively, indicating that Toona ci/iata is a stable growth-oriented population with high risk sensitivity. The survival rate curve was approximately fitted to the Deevey II type, so the individuals in each age class had an equivalent mortality rate in overall. The qx and Kx all reached peaks si- multaneously at 1, 4 and 6 age classes, indicating great effects of physiological properties, environmental sieves and human disturbances on Toona ci/iata population at various growth stages. The spectral analysis showed that the fundamental wave A1 was 0.752 8, while A3 showed an obvious minor cycle deviating from AI. It suggests that there is a periodicity, with minor cycles of multi-harmonic superposition, in natural regeneration of Toona ciliata population.展开更多
In order to research the population distribution pattern of endangered species Toona ciliata Roem., the sampling quadrats of 5 mx5 m and 3 m×3 m in size, accurate to 1 mxl m, were established in 2 newly-found onl...In order to research the population distribution pattern of endangered species Toona ciliata Roem., the sampling quadrats of 5 mx5 m and 3 m×3 m in size, accurate to 1 mxl m, were established in 2 newly-found only existing T. cili- ata Roem. communities （T1 and T2） with contiguous grid quadrate method, in the Nanhe River valley, Gucheng County. By X2 test, t-test of distribution coefficient Cx method, and F test of Morisita pattern index Iδ whether the distribution patterns of the T. ciliata Roem. populations conformed to Poisson distribution were checked. The results indicated that, population T1 was in Poisson distribution under 5 m×5 m and 3 m×3 m in size by Cx and 16 methods, but in clumped distribution pattern un- der 5 m×5 m in size by the Chi-square test; however, the population was in Pois- son distribution under the dimension of 3 m×3 m. Population T2 under human dis- turbance had higher population density, indicating clumped distribution under 3 above-mentioned tests. If Chi-square test is satisfied, a distribution pattern is in Poisson distribution, and size and quantity of sampling quadrats should be given pri- ority to; and if df is greater, both theoretical values and observed values tend to- wards normal distribution more probably, and the test of distribution pattern, there- fore will be more dependable.展开更多
The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and...The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and herb layers of eight natural communities of Toona ciliata as research targets,three diff erent ecological niche models were used:broken stick model,overlapping niche model and niche preemption model,as well as three statistical models:log-series distribution model,log-normal distribution model and Weibull distribution model,to fi t SAD of the diff erent vegetation layers based on data collected.Goodness-of-fi t was compared with Chi square test,Kolmogorov–Smirnov(K–S)test and Akaike Information Criterion(AIC).The results show:(1)based on the criteria of the lowest AIC value,Chi square value and K–S value with no signifi cant diff erence(p>0.05)between theoretic and observed SADs.The suitability and goodness-of-fi t of the broken stick model was the best of three ecological niche models.The log-series distribution model did not accept the fi tted results of most vegetation layers and had the lowest goodness-of-fi t.The Weibull distribution model had the best goodness-of-fi t for SADs.Overall,the statistical SADs performed better than the ecological ones.(2)T.ciliata was the dominant species in all the communities;species richness and diversity of herbs were the highest of the vegetation layers,while the diversities of the tree layers were slightly higher than the shrub layers;there were fewer common species and more rare species in the eight communities.The herb layers had the highest community evenness,followed by the shrub and the tree layers.Due to the complexity and habitat diversity of the diff erent T.ciliata communities,comprehensive analyses of a variety of SADs and tests for optimal models together with management,are practical steps to enhance understanding of ecological processes and mechanisms of T.ciliata communities,to detect disturbances,and to facilitate biodiversity and species conservation.展开更多
We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effe...We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants.展开更多
In this study, the comparison of Elsholtzia ciliata volatile compounds from the stem (with leaf) and flower was acquired. The volatile compounds of these two parts from Elsholtzia ciliata aerial parts were respectivel...In this study, the comparison of Elsholtzia ciliata volatile compounds from the stem (with leaf) and flower was acquired. The volatile compounds of these two parts from Elsholtzia ciliata aerial parts were respectively analyzed by a rapid and convenient static headspace injection technique coupled with gas chromatography-mass spectrometry and the relative contents of each constituent between the two different parts were determined by peak area normalization. 61 compounds were identified in the stem (with leaf) and flower, among which 39 components simultaneously exist. 47 and 53 compounds were separated and identified in the volatile compounds from the different parts of Elsholtzia ciliata, respectively. There were differences among some volatile constituents of the two parts, but the main constituents were all Elsholtzia ketone, caryophyllene, 3-octanol and Dehydroelsholtzia ketone. The most abundant components in the stem (with leaf) included Elsholtzia ketone (84.20%), caryophyllene (4.3%) and 3-octanol (3.11%), while Elsholtzia ketone (88.03%), caryophyllene (3.33%) and 3-octanol (1.53%) were the main components in the flower. 8 single constituents were identified in the stem (with leaf) volatiles including 3-heptanone and linalool, while 14 constituents only including 2-methylbutanoic acid and Perillene have been found in the flower volatiles. Elsholtzia ketone was the main compound, and its concentration was significantly higher than other substances (>80% of the total oils in Elsholtzia ciliata). The study provided an important scientific base for the further utilization of Elsholtzia ciliata resources and may be helpful for systematically understanding the constituents of volatile compounds of Elsholtzia ciliata.展开更多
A glasshouse experiment using a rhizobox technique was conducted to examine salt dynamics in the rhizosphere of a salt-tolerant grass, Puccinellia ciliata Bor. ’Irwin Hunter’, grown in a loamy soil, and to study the...A glasshouse experiment using a rhizobox technique was conducted to examine salt dynamics in the rhizosphere of a salt-tolerant grass, Puccinellia ciliata Bor. ’Irwin Hunter’, grown in a loamy soil, and to study the effect of rainfall flush on salt accumulation in the rhizosphere. The rhizobox (10 × 5.5 × 50 cm) had a nylon mesh (1 μm) positioned vertically in the middle to create two compartments filled with soil amended with 1 g NaCl kg-1. The plants were grown in one compartment only. Flushed treatments received 275 mL of deionized water two days before harvest. In the plant-growing compartment, soils were sectioned vertically at 5 cm intervals. Significant differences in soil electrical conductivity (EC) (P < 0.05) and pH (P < 0.05) were observed for depths, but not between flushed and non-flushed treatments. In the no-plant compartment (rhizosphere), soil cores were taken horizontally at depths of 5, 20 and 40 cm and sliced at 1, 2, 3, 4, 5, 7, 10, 15 and 20 mm away from the roots. Soil EC and Cl- concentration at the 5 and 20 cm depths, and Na+ concentration at the 5 cm depth significantly decreased (P < 0.05) with the distance away from the root, but no significant differences were observed in soil pH and concentrations of the K+ and Ca2+. The flush treatment only had significant influence on soil EC, pH, and Cl- concentration at the 20 cm depth. Thus, salt accumulation could occur in the rhizosphere of salt-tolerant species on saline soils, and the periodic low rainfall might not have a strong influence on salt distribution in the rhizosphere and/or root zone.展开更多
Dear Editor： Therapeutically active metabolite contents in a med- icinal plant vary in nature, which may impact on its therapeutic efficacy. Bergenia （Saxifragaceae） is an evergreen perennial herb widely distribut...Dear Editor： Therapeutically active metabolite contents in a med- icinal plant vary in nature, which may impact on its therapeutic efficacy. Bergenia （Saxifragaceae） is an evergreen perennial herb widely distributed in Central and East Asia with about 30 species reported worldwide. It grows at a range of altitudes from the Khasia hills at 400 feet to the temperate Himalayas from Kashmir to Bhutan at 7,000-10,000 feet. Its distribution over a wide range of altitudinal zones makes it a good candidate for studying variations in its metabolic profiles under different climatic conditions. Bergenin （C-glycoside of 4-O-methyl gallic acid） has been identified as a potent active secondary metabolite in Bergenia and other therapeutically active constituents including, among others, gallic acid （3,4,5 trihydroxybenzoic acid）, （＋） catechin, and gallicin （Fig. 1）.展开更多
[Objectives]To determine the optimum technological conditions of ultrasound-assisted extraction of polysaccharides from Ajuga ciliata Bunge.[Methods]In this study,Ajuga ciliata Bunge was used as raw material,and an or...[Objectives]To determine the optimum technological conditions of ultrasound-assisted extraction of polysaccharides from Ajuga ciliata Bunge.[Methods]In this study,Ajuga ciliata Bunge was used as raw material,and an orthogonal experiment was designed based on the single factor experiment to investigate the effects of ultrasonic time,solid-liquid ratio,ultrasonic temperature and ultrasonic power on the extraction rate of polysaccharides from A.ciliata Bunge(Fujian).Range analysis,variance analysis and SSR test of significant differences between groups were carried out with SPSS 20.0,and the test results were verified.[Results]Under the conditions of ultrasonic temperature of 85℃,ultrasonic time of 80 min,solid-liquid ratio of 1∶15 g/mL and ultrasonic power of 500 W,the yield of polysaccharides from A.ciliata Bunge(Fujian)was the best.[Conclusions]This study can provide theoretical basis for the application of A.ciliata Bunge(Fujian).展开更多
The flight capacity of different ages and sexes of the sycamore lace bug, Corythucha ciliata, was studied at different temperatures using a flight mill system. The results of regression analysis showed a significant e...The flight capacity of different ages and sexes of the sycamore lace bug, Corythucha ciliata, was studied at different temperatures using a flight mill system. The results of regression analysis showed a significant effect of temperature on flight distance (P=0.0082). Temperature did not influence flight duration (P=0.212) or flight speed (P=0.175). The mean flight distance (1 024 m) and mean flight duration were the greatest at 25.2°C. The age of C. ciliata had a significant influence on flight distance (P=0.0005), flight duration (P=0.0005) and flight speed (P=0.026). The 12-d-old adult had the greatest flight distance (887 m), duration (3 875 s) and speed (0.22 m s^–1). Flight distances and flight duration of females were significantly longer than that of males. However, the male had significantly greater flight speed than the female. The insect appears to be capable of long distance flights. The understanding of the optimal age and temperature for the flight of this insect through this study provides a foundation for better management of the insect in China.展开更多
Most ecological studies in North Africa reveal a process of continuous degradation of rangeland ecosystems as a result of overgrazing. This degradation appears across the decreasing of perennial grass diversity. Indee...Most ecological studies in North Africa reveal a process of continuous degradation of rangeland ecosystems as a result of overgrazing. This degradation appears across the decreasing of perennial grass diversity. Indeed, the majority of steppe ecosystems are characterized by a low density of perennial grass species at present. This study evaluated the effects of temperature and water limitation on the seed germination of Stipagrostis ciliata（Desf.） de Winter, a perennial grass species. The seeds were collected from the Bou Hedma Park, Sidi Bouzid Governorate, Central Tunisia. The thermal time and hydrothermal time models were used to describe the seed germination of S. ciliata under different water potentials and temperatures. The germination response of S. ciliata seeds in darkness was evaluated over a range of temperatures（15°C, 20°C, 25°C, 30°C and 35°C） and across a wide range of osmotic potentials（0.0, –0.2, –0.6, –1.2, –1.6 and –2.0 MPa） of the polyethylene glycol（PEG6000） solutions at each temperature level. Among the tested temperatures, 25°C was found to be the optimal temperature to the germination of S. ciliata seeds. The final germination percentage（75.2%） was obtained with distilled water. The progressive decrease of osmotic potential of the PEG6000 solutions inhibited the seed germination. However, the number of days to first germination was increased with a reduction of osmotic potential. A significant positive relationship was identified between final germination percentage of S. ciliata seeds and osmotic potential of the PEG6000 solutions, with R^2 ranging from 0.5678 to 0.8761. Furthermore, a high degree of congruency between predicted and observed germination time course curves was observed. In general, S. ciliata exhibits a significant adaptation capacity for water limitation and high temperature in arid ecosystems.展开更多
Stipagrostis ciliata(Desf.)De Winter is a pastoral C4 grass grown in arid regions.This research work focused on assessing the growth of S.ciliata accessions derived from two different climate regions(a wet arid region...Stipagrostis ciliata(Desf.)De Winter is a pastoral C4 grass grown in arid regions.This research work focused on assessing the growth of S.ciliata accessions derived from two different climate regions(a wet arid region in the Bou Hedma National Park in the central and southern part of Tunisia(coded as WA),and a dry arid region from the Matmata Mountain in the south of Tunisia(coded as DA))under water stress conditions.Specifically,the study aimed to investigate the phenological and physiological responses of potted S.ciliata seedlings under different water treatments:T_(1)(200 mm/a),T_(2)(150 mm/a),T_(3)(100 mm/a)and T_(4)(50 mm/a).Growth phenology,net photosynthesis(Pn),stomatal conductance(gs),midday leaf water potential(Ψmd),predawn leaf water potential(Ψpd),soil water content(SWC)and soil water potential(Ψs)were observed during the water stress cycle(from December 2016 to November 2017).The obtained results showed that the highest growth potential of the two accessions(WA and DA)was recorded under treatment T_(1).The two accessions responded differently and significantly to water stress.Photosynthetic parameters,such as Pn and gs,decreased sharply under treatments T_(2),T_(3)and T_(4)compared to treatment T_(1).The higher water stress increased the R/S ratio(the ratio of root dry biomass to shoot dry biomass),with values of 1.29 and 2.74 under treatment T_(4)for accessions WA and DA,respectively.Principal component analysis(PCA)was applied,and the separation of S.ciliata accessions on the first two axes of PCA(PC1 and PC2)suggested that accession DA was detected in the negative extremity of PC1 and PC2 under treatments T_(1)and T_(2).This accession was characterized by a high number of spikes.For treatments T_(3)and T_(4),both accessions were detected in the negative extremity of PC1 and PC2.They were characterized by a high root dry biomass.Therefore,S.ciliata accessions responded to water stress by displaying significant changes in their behaviours.Accession WA from the Bou Hedma National Park(wet arid region)showed higher drought tolerance than accession DA from the Matmata Mountain(dry arid region).S.ciliata exhibits a significant adaptation capacity for water limitation and may be an important species for ecosystem restoration.展开更多
Through two years of systematic investigation,it was found that Corythucha ciliata occurred in the main streets of Guangde City and spread rapidly to roads and streets of some villages and towns.The investigation foun...Through two years of systematic investigation,it was found that Corythucha ciliata occurred in the main streets of Guangde City and spread rapidly to roads and streets of some villages and towns.The investigation found that C.ciliata could harm indigenous tree species such as Broussonetia papyrifera,and could also quickly occupy the living space of thrips,the original leaf pest of local Platanus acerifolia.Literature review also suggested that the pest would also be a potential threat to indigenous tree species,such as Rhododendron simsii Planch,Prunus cerasifera Ehrhar,etc.Guangde City,located in the middle and lower reaches of the Yangtze River,was sunny,hot and dry in summer from 2021 to 2022.However,C.ciliata had no obvious diapause habits in summer,with strong adaptability.It was easy to control C.ciliata,but was difficult to eradicate the pest.According to the risk analysis index system of dangerous forest pests,the risk value of C.ciliata was determined as R=1.80,belonging to moderately dangerous pest in Guangde.Through quarantine,publicity,winter and summer management measures,the occurrence of C.ciliata was controlled to a certain extent,and the harm and transmission risk of the pest were reduced.展开更多
Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give ...Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy(TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.展开更多
基金Supported by Public Welfare Research Projects of Science and Technology Bureau of Hubei Province(40 2012DBA40001)~~
文摘Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.
基金Supported by Hunan Forestry Science and Technology Project(XLK201406)~~
文摘Cutting propagation rhizogenesis of Toona ciliata clone cutting orchard were studied, and the results were as fol ows： Rooting type included both cal us rooting and cortex rooting. About 17 in 22 of clones were dominated by cortex rooting, accounting for the 77% of clones. There were 5 clones which occurred with both cal us rooting and cortex rooting during rooting process. The rooting efficiency index （REI） showed a positive correlation with the rooting rate. Under natural tem-perature condition, keeping moisture and shading, we found that the highest survival rate occurred in early June, September, and October.
文摘Populus species are important resources for industry and in scientific study on biological and agricul- tural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar （Populus ciliata wall. ex Royle）. The effect of TDZ alone and in combi- nation with adenine and NAA was studied on the regen- eration potential of petiole explants. The explants were excised from Himalayan poplar plants grown in glass- houses. After surface sterilization the explants were cul- tured on shoot induction medium. High percentage shoot regeneration （86 %） was recorded on MS medium sup- plemented with 0.004 mg L-1 TDZ and 79.7 mg L-1 adenine. The regenerated shoots for elongation and multi- plication were transferred to MS ＋ 0.5 mg L-1 BAP ＋ 0.2 mg L-1 IAA ＋ 0.3 mg L-1 GA3. Root re- generation from shoots developed in vitro was observed on MS medium supplemented with 0.10 mg L-1 IBA. Hi- malayan poplar plantlets could be produced within 2 months after acclimatization in a sterile mixture of sand and soil. We developed a high efficiency plant regeneration protocol from petiole explants of P. ciliata.
基金Supported by Special Scientific Research Fund of Public Welfare Profession of Department of Science and Technology of Hubei Province(40 2012DBA40001)~~
文摘To investigate the population dynamics of Toona ciliata in Qizimeishan Na- ture Reserve, the age classes and population quantity of Toona clliata in Qizimeishan were analyzed by transforming diameter classes into corresponding age classes. A static life table was prepared. Moreover, the curves of survival rate, mortality rate and killing powder were drawn so as to perform spectral analysis for Toona clliata in Qizimeishan Nature Reserve. The results showed that the Vpi and Vpf of the Toona ci/iata population were 32.32% and 4.039%, respectively, indicating that Toona ci/iata is a stable growth-oriented population with high risk sensitivity. The survival rate curve was approximately fitted to the Deevey II type, so the individuals in each age class had an equivalent mortality rate in overall. The qx and Kx all reached peaks si- multaneously at 1, 4 and 6 age classes, indicating great effects of physiological properties, environmental sieves and human disturbances on Toona ci/iata population at various growth stages. The spectral analysis showed that the fundamental wave A1 was 0.752 8, while A3 showed an obvious minor cycle deviating from AI. It suggests that there is a periodicity, with minor cycles of multi-harmonic superposition, in natural regeneration of Toona ciliata population.
基金Supported by Public Welfare Research Project of Science and Technology Agency of Hubei Province(402012DBA40001)~~
文摘In order to research the population distribution pattern of endangered species Toona ciliata Roem., the sampling quadrats of 5 mx5 m and 3 m×3 m in size, accurate to 1 mxl m, were established in 2 newly-found only existing T. cili- ata Roem. communities （T1 and T2） with contiguous grid quadrate method, in the Nanhe River valley, Gucheng County. By X2 test, t-test of distribution coefficient Cx method, and F test of Morisita pattern index Iδ whether the distribution patterns of the T. ciliata Roem. populations conformed to Poisson distribution were checked. The results indicated that, population T1 was in Poisson distribution under 5 m×5 m and 3 m×3 m in size by Cx and 16 methods, but in clumped distribution pattern un- der 5 m×5 m in size by the Chi-square test; however, the population was in Pois- son distribution under the dimension of 3 m×3 m. Population T2 under human dis- turbance had higher population density, indicating clumped distribution under 3 above-mentioned tests. If Chi-square test is satisfied, a distribution pattern is in Poisson distribution, and size and quantity of sampling quadrats should be given pri- ority to; and if df is greater, both theoretical values and observed values tend to- wards normal distribution more probably, and the test of distribution pattern, there- fore will be more dependable.
基金Hubei Provincial Department of Science and Technology,under the public welfare research project[No.402012DBA40001]Hubei Provincial Department of Education,under the scientifi c research project[No.B20160555].
文摘The study of plant species abundance distribution(SAD)in natural communities is of considerable importance to understand the processes and ecological rules of community assembly.With the distribution of tree,shrub and herb layers of eight natural communities of Toona ciliata as research targets,three diff erent ecological niche models were used:broken stick model,overlapping niche model and niche preemption model,as well as three statistical models:log-series distribution model,log-normal distribution model and Weibull distribution model,to fi t SAD of the diff erent vegetation layers based on data collected.Goodness-of-fi t was compared with Chi square test,Kolmogorov–Smirnov(K–S)test and Akaike Information Criterion(AIC).The results show:(1)based on the criteria of the lowest AIC value,Chi square value and K–S value with no signifi cant diff erence(p>0.05)between theoretic and observed SADs.The suitability and goodness-of-fi t of the broken stick model was the best of three ecological niche models.The log-series distribution model did not accept the fi tted results of most vegetation layers and had the lowest goodness-of-fi t.The Weibull distribution model had the best goodness-of-fi t for SADs.Overall,the statistical SADs performed better than the ecological ones.(2)T.ciliata was the dominant species in all the communities;species richness and diversity of herbs were the highest of the vegetation layers,while the diversities of the tree layers were slightly higher than the shrub layers;there were fewer common species and more rare species in the eight communities.The herb layers had the highest community evenness,followed by the shrub and the tree layers.Due to the complexity and habitat diversity of the diff erent T.ciliata communities,comprehensive analyses of a variety of SADs and tests for optimal models together with management,are practical steps to enhance understanding of ecological processes and mechanisms of T.ciliata communities,to detect disturbances,and to facilitate biodiversity and species conservation.
基金This work was fi nancially supported by the National Key Research Projects,Forestry Resource Cultivation and Utilization Technology Innovation(Grant No.2016YFD0600606)the Natural Science Foundation of Guangdong Province of China(Grant No.2018A030313798)Characteristic innovation projects of department of education of Guangdong province(Grant No.2019KTSCX017).
文摘We used the cotyledons and cotyledonary nodes of Toona ciliata(Chinese mahogany)as explants to examine callus and adventitious shoot induction when exposed to different ratios of hormones.We also investigated the effects of seedling age,inoculation method,and genotype on the efficient regeneration of T.ciliata.The results showed that different genotypes exhibited significantly different callus induction efficiency.The cotyledons and cotyledonary nodes of 20-day seedlings inoculated onto MS medium with 0.5 mg/L 6-benzylaminopurine(6-BA),0.5 mg/L kinetin(KT)and 0.05 mg/L 1-naphthylacetic acid(NAA)achieved a greater regeneration rate than did other concentrations of cytokinin and auxin.The numbers of shoots per cotyledon and cotyledonary node explant were 7.33 and 6.67.The optimal inoculation method for cotyledons was that the distal end of the explants was placed in contact with the medium.The optimal adventitious shoot differentiation medium for cotyledon explants was MS medium containing 0.3 mg/L 6-BA and 0.2 mg/L NAA,producing a 3.4 cm height of shoot on average.This study established an efficient regeneration system for T.ciliata with cotyledons and cotyledonary nodes as explants.
文摘In this study, the comparison of Elsholtzia ciliata volatile compounds from the stem (with leaf) and flower was acquired. The volatile compounds of these two parts from Elsholtzia ciliata aerial parts were respectively analyzed by a rapid and convenient static headspace injection technique coupled with gas chromatography-mass spectrometry and the relative contents of each constituent between the two different parts were determined by peak area normalization. 61 compounds were identified in the stem (with leaf) and flower, among which 39 components simultaneously exist. 47 and 53 compounds were separated and identified in the volatile compounds from the different parts of Elsholtzia ciliata, respectively. There were differences among some volatile constituents of the two parts, but the main constituents were all Elsholtzia ketone, caryophyllene, 3-octanol and Dehydroelsholtzia ketone. The most abundant components in the stem (with leaf) included Elsholtzia ketone (84.20%), caryophyllene (4.3%) and 3-octanol (3.11%), while Elsholtzia ketone (88.03%), caryophyllene (3.33%) and 3-octanol (1.53%) were the main components in the flower. 8 single constituents were identified in the stem (with leaf) volatiles including 3-heptanone and linalool, while 14 constituents only including 2-methylbutanoic acid and Perillene have been found in the flower volatiles. Elsholtzia ketone was the main compound, and its concentration was significantly higher than other substances (>80% of the total oils in Elsholtzia ciliata). The study provided an important scientific base for the further utilization of Elsholtzia ciliata resources and may be helpful for systematically understanding the constituents of volatile compounds of Elsholtzia ciliata.
文摘A glasshouse experiment using a rhizobox technique was conducted to examine salt dynamics in the rhizosphere of a salt-tolerant grass, Puccinellia ciliata Bor. ’Irwin Hunter’, grown in a loamy soil, and to study the effect of rainfall flush on salt accumulation in the rhizosphere. The rhizobox (10 × 5.5 × 50 cm) had a nylon mesh (1 μm) positioned vertically in the middle to create two compartments filled with soil amended with 1 g NaCl kg-1. The plants were grown in one compartment only. Flushed treatments received 275 mL of deionized water two days before harvest. In the plant-growing compartment, soils were sectioned vertically at 5 cm intervals. Significant differences in soil electrical conductivity (EC) (P < 0.05) and pH (P < 0.05) were observed for depths, but not between flushed and non-flushed treatments. In the no-plant compartment (rhizosphere), soil cores were taken horizontally at depths of 5, 20 and 40 cm and sliced at 1, 2, 3, 4, 5, 7, 10, 15 and 20 mm away from the roots. Soil EC and Cl- concentration at the 5 and 20 cm depths, and Na+ concentration at the 5 cm depth significantly decreased (P < 0.05) with the distance away from the root, but no significant differences were observed in soil pH and concentrations of the K+ and Ca2+. The flush treatment only had significant influence on soil EC, pH, and Cl- concentration at the 20 cm depth. Thus, salt accumulation could occur in the rhizosphere of salt-tolerant species on saline soils, and the periodic low rainfall might not have a strong influence on salt distribution in the rhizosphere and/or root zone.
文摘Dear Editor： Therapeutically active metabolite contents in a med- icinal plant vary in nature, which may impact on its therapeutic efficacy. Bergenia （Saxifragaceae） is an evergreen perennial herb widely distributed in Central and East Asia with about 30 species reported worldwide. It grows at a range of altitudes from the Khasia hills at 400 feet to the temperate Himalayas from Kashmir to Bhutan at 7,000-10,000 feet. Its distribution over a wide range of altitudinal zones makes it a good candidate for studying variations in its metabolic profiles under different climatic conditions. Bergenin （C-glycoside of 4-O-methyl gallic acid） has been identified as a potent active secondary metabolite in Bergenia and other therapeutically active constituents including, among others, gallic acid （3,4,5 trihydroxybenzoic acid）, （＋） catechin, and gallicin （Fig. 1）.
基金Supported by Fujian College Students Innovation and Entrepreneurship Training Program in 2019(201912631026)Innovation and Entrepreneurship Training Program for College Students of Xiamen Medical College(201812631052,201912631057).
文摘[Objectives]To determine the optimum technological conditions of ultrasound-assisted extraction of polysaccharides from Ajuga ciliata Bunge.[Methods]In this study,Ajuga ciliata Bunge was used as raw material,and an orthogonal experiment was designed based on the single factor experiment to investigate the effects of ultrasonic time,solid-liquid ratio,ultrasonic temperature and ultrasonic power on the extraction rate of polysaccharides from A.ciliata Bunge(Fujian).Range analysis,variance analysis and SSR test of significant differences between groups were carried out with SPSS 20.0,and the test results were verified.[Results]Under the conditions of ultrasonic temperature of 85℃,ultrasonic time of 80 min,solid-liquid ratio of 1∶15 g/mL and ultrasonic power of 500 W,the yield of polysaccharides from A.ciliata Bunge(Fujian)was the best.[Conclusions]This study can provide theoretical basis for the application of A.ciliata Bunge(Fujian).
基金funded by the Fundamental Research Funds of Henan Academy of Forestry, China (162102410033)
文摘The flight capacity of different ages and sexes of the sycamore lace bug, Corythucha ciliata, was studied at different temperatures using a flight mill system. The results of regression analysis showed a significant effect of temperature on flight distance (P=0.0082). Temperature did not influence flight duration (P=0.212) or flight speed (P=0.175). The mean flight distance (1 024 m) and mean flight duration were the greatest at 25.2°C. The age of C. ciliata had a significant influence on flight distance (P=0.0005), flight duration (P=0.0005) and flight speed (P=0.026). The 12-d-old adult had the greatest flight distance (887 m), duration (3 875 s) and speed (0.22 m s^–1). Flight distances and flight duration of females were significantly longer than that of males. However, the male had significantly greater flight speed than the female. The insect appears to be capable of long distance flights. The understanding of the optimal age and temperature for the flight of this insect through this study provides a foundation for better management of the insect in China.
基金supported by the Tunisian Ministry of Scientific Research, especially the arid lands program
文摘Most ecological studies in North Africa reveal a process of continuous degradation of rangeland ecosystems as a result of overgrazing. This degradation appears across the decreasing of perennial grass diversity. Indeed, the majority of steppe ecosystems are characterized by a low density of perennial grass species at present. This study evaluated the effects of temperature and water limitation on the seed germination of Stipagrostis ciliata（Desf.） de Winter, a perennial grass species. The seeds were collected from the Bou Hedma Park, Sidi Bouzid Governorate, Central Tunisia. The thermal time and hydrothermal time models were used to describe the seed germination of S. ciliata under different water potentials and temperatures. The germination response of S. ciliata seeds in darkness was evaluated over a range of temperatures（15°C, 20°C, 25°C, 30°C and 35°C） and across a wide range of osmotic potentials（0.0, –0.2, –0.6, –1.2, –1.6 and –2.0 MPa） of the polyethylene glycol（PEG6000） solutions at each temperature level. Among the tested temperatures, 25°C was found to be the optimal temperature to the germination of S. ciliata seeds. The final germination percentage（75.2%） was obtained with distilled water. The progressive decrease of osmotic potential of the PEG6000 solutions inhibited the seed germination. However, the number of days to first germination was increased with a reduction of osmotic potential. A significant positive relationship was identified between final germination percentage of S. ciliata seeds and osmotic potential of the PEG6000 solutions, with R^2 ranging from 0.5678 to 0.8761. Furthermore, a high degree of congruency between predicted and observed germination time course curves was observed. In general, S. ciliata exhibits a significant adaptation capacity for water limitation and high temperature in arid ecosystems.
基金This research was supported by Tunisian Minister of Research and High Education in particular Laboratory of Ecosystems and Biodiversity in Arid Land of Tunisia(LEBIOMAT),University of Sfax.
文摘Stipagrostis ciliata(Desf.)De Winter is a pastoral C4 grass grown in arid regions.This research work focused on assessing the growth of S.ciliata accessions derived from two different climate regions(a wet arid region in the Bou Hedma National Park in the central and southern part of Tunisia(coded as WA),and a dry arid region from the Matmata Mountain in the south of Tunisia(coded as DA))under water stress conditions.Specifically,the study aimed to investigate the phenological and physiological responses of potted S.ciliata seedlings under different water treatments:T_(1)(200 mm/a),T_(2)(150 mm/a),T_(3)(100 mm/a)and T_(4)(50 mm/a).Growth phenology,net photosynthesis(Pn),stomatal conductance(gs),midday leaf water potential(Ψmd),predawn leaf water potential(Ψpd),soil water content(SWC)and soil water potential(Ψs)were observed during the water stress cycle(from December 2016 to November 2017).The obtained results showed that the highest growth potential of the two accessions(WA and DA)was recorded under treatment T_(1).The two accessions responded differently and significantly to water stress.Photosynthetic parameters,such as Pn and gs,decreased sharply under treatments T_(2),T_(3)and T_(4)compared to treatment T_(1).The higher water stress increased the R/S ratio(the ratio of root dry biomass to shoot dry biomass),with values of 1.29 and 2.74 under treatment T_(4)for accessions WA and DA,respectively.Principal component analysis(PCA)was applied,and the separation of S.ciliata accessions on the first two axes of PCA(PC1 and PC2)suggested that accession DA was detected in the negative extremity of PC1 and PC2 under treatments T_(1)and T_(2).This accession was characterized by a high number of spikes.For treatments T_(3)and T_(4),both accessions were detected in the negative extremity of PC1 and PC2.They were characterized by a high root dry biomass.Therefore,S.ciliata accessions responded to water stress by displaying significant changes in their behaviours.Accession WA from the Bou Hedma National Park(wet arid region)showed higher drought tolerance than accession DA from the Matmata Mountain(dry arid region).S.ciliata exhibits a significant adaptation capacity for water limitation and may be an important species for ecosystem restoration.
文摘Through two years of systematic investigation,it was found that Corythucha ciliata occurred in the main streets of Guangde City and spread rapidly to roads and streets of some villages and towns.The investigation found that C.ciliata could harm indigenous tree species such as Broussonetia papyrifera,and could also quickly occupy the living space of thrips,the original leaf pest of local Platanus acerifolia.Literature review also suggested that the pest would also be a potential threat to indigenous tree species,such as Rhododendron simsii Planch,Prunus cerasifera Ehrhar,etc.Guangde City,located in the middle and lower reaches of the Yangtze River,was sunny,hot and dry in summer from 2021 to 2022.However,C.ciliata had no obvious diapause habits in summer,with strong adaptability.It was easy to control C.ciliata,but was difficult to eradicate the pest.According to the risk analysis index system of dangerous forest pests,the risk value of C.ciliata was determined as R=1.80,belonging to moderately dangerous pest in Guangde.Through quarantine,publicity,winter and summer management measures,the occurrence of C.ciliata was controlled to a certain extent,and the harm and transmission risk of the pest were reduced.
基金supported by the National Key Technology Support Program (2011BAD13B05)
文摘Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy(TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.
