For the first time,a nanoformulated fungicide carboxin was obtained to solve the problem of infection of potato tubers with fungi spores.Encapsulation of carboxin in ultra-deformable liposomes protects it from degrada...For the first time,a nanoformulated fungicide carboxin was obtained to solve the problem of infection of potato tubers with fungi spores.Encapsulation of carboxin in ultra-deformable liposomes protects it from degradation by sunlight,increases solubility in water and penetration through potato skin.Two fluorescent probes,rhodamine B and fluorescein,were used for optimization of original composition and for visualization of the transfersome localization in potato tubers,respectively.Carboxin was encapsulated in lipid formulations with further evaluation of encapsulation efficiency,substrate release rate in vitro,and penetration through the Strat-M®membrane using Franz cells.Using fluorescent microscopy,it was revealed that transfersomes penetrate through the periderm and enter cortex of potato tubers.By the extraction it was shown that potato tubers weighing 100 g included 32 mg of carboxin after the transfersome treatment.The inclusion of carboxin in transfersomes made it possible to increase the maximum concentration of carboxin in water from 0.57 mmol L^(−1) to 2.8–3.8 mmol L^(−1),i.e.,5–7 times.展开更多
Virulence of seedvax (amicarthiazol), saikuzuo (bismerthiazol) and carboxin to Xanthomonas citri、X. oryzae pv. oryzae、X.campestris pv. glycines and Rhizoctonia cerealis were determined , and the relation of their ac...Virulence of seedvax (amicarthiazol), saikuzuo (bismerthiazol) and carboxin to Xanthomonas citri、X. oryzae pv. oryzae、X.campestris pv. glycines and Rhizoctonia cerealis were determined , and the relation of their activity with their composition were analysised , Furthermore, their effects on growth, respiration and fungiactive mode to X. citri were taken into investigation . The results indicated that virulence mechanism of seedvax is interrelated to that of saikuzuo and carboxin , seedvax possibly attack any targets of the respiration pathway and other unknown bio chemical sites, seedvax is characterized with poly active location when transported in bio metabolic ways.展开更多
基金financial support from the government assignment for FRC Kazan Scientific Center of RAS in part of the sample preparation and characterization for LAV,GAG,LYaZ,in part of visualization of nanoparticles for IRN,MKK,and in part of in vitro penetration study by fluorescence microscopy for AAP,TAG.
文摘For the first time,a nanoformulated fungicide carboxin was obtained to solve the problem of infection of potato tubers with fungi spores.Encapsulation of carboxin in ultra-deformable liposomes protects it from degradation by sunlight,increases solubility in water and penetration through potato skin.Two fluorescent probes,rhodamine B and fluorescein,were used for optimization of original composition and for visualization of the transfersome localization in potato tubers,respectively.Carboxin was encapsulated in lipid formulations with further evaluation of encapsulation efficiency,substrate release rate in vitro,and penetration through the Strat-M®membrane using Franz cells.Using fluorescent microscopy,it was revealed that transfersomes penetrate through the periderm and enter cortex of potato tubers.By the extraction it was shown that potato tubers weighing 100 g included 32 mg of carboxin after the transfersome treatment.The inclusion of carboxin in transfersomes made it possible to increase the maximum concentration of carboxin in water from 0.57 mmol L^(−1) to 2.8–3.8 mmol L^(−1),i.e.,5–7 times.
文摘Virulence of seedvax (amicarthiazol), saikuzuo (bismerthiazol) and carboxin to Xanthomonas citri、X. oryzae pv. oryzae、X.campestris pv. glycines and Rhizoctonia cerealis were determined , and the relation of their activity with their composition were analysised , Furthermore, their effects on growth, respiration and fungiactive mode to X. citri were taken into investigation . The results indicated that virulence mechanism of seedvax is interrelated to that of saikuzuo and carboxin , seedvax possibly attack any targets of the respiration pathway and other unknown bio chemical sites, seedvax is characterized with poly active location when transported in bio metabolic ways.
