Carbonic anhydrase Ⅱ (CAⅡ) is an important enzyme complex with Zn2+,which is involved in many physiological and pathological processes, such as calcification, glaucoma and tumorigenicity. In order to search for nove...Carbonic anhydrase Ⅱ (CAⅡ) is an important enzyme complex with Zn2+,which is involved in many physiological and pathological processes, such as calcification, glaucoma and tumorigenicity. In order to search for novel inhibitors of CAⅡ, inhibition assay of carbonic anhydrase Ⅱ was performed, by which seven natural phenolic compounds, including four phenolics (grifolin, 4-O-methyl-grifolic acid, grifolic acid, and isovanillic acid) and three flavones (eriodictyol, quercetin and puerin A), showed in-hibitory activities against CAⅡ with IC50s in the range of 6.37-71.73 μmol/L. Grifolic acid is the most active one with IC50 of 6.37 μmol/L. These seven phenolic compounds were proved to be novel natural carbonic anhydrase Ⅱ inhibitors, which were obtained in flexible docking study with GOLD 3.0 soft-ware. Results indicated that the aliphatic chain and polar groups of hydroxyl and carboxyl are impor-tant to their inhibitory activities, providing a new insight into study on CA Ⅱ potent inhibitors.展开更多
If the photosynthetic organisms assimilated only CO_(2) in the Archean atmosphere,hydroxide ion in the Archean seawater would not increase.If plants would not consume bicarbonate as a direct substrate during photosynt...If the photosynthetic organisms assimilated only CO_(2) in the Archean atmosphere,hydroxide ion in the Archean seawater would not increase.If plants would not consume bicarbonate as a direct substrate during photosynthesis,it is difficult to explain the evolution of Earth's environment.To date,it is generally accepted that photosynthetic O_(2) evolution of plants come from water photolysis.However,it should be debated by evaluating the effect of bicarbonate in photosynthetic O_(2) evolution,analyzing the role of carbonic anhydrase(CA) in photosynthetic O_(2) evolution,and the relationship between thylakoid CA and photosynthetic O_(2) evolution.In the paper,I propose that bicarbonate is directly used as substrate to participate in photosynthetic O_(2) evolution.The rationality of bicarbonate photolysis of plants is discussed from the thermodynamics and evolution of Earth's environment.The isotopic evidence that bicarbonate is not the direct substrate of photosynthetic O_(2) release is reexamined,and the new explanation of bicarbonate photolysis in photosynthetic O_(2) evolution is proposed.展开更多
基金Supported by the National Natural Science Foundation of China (Grant No. 30725048)the Foundation of Chinese Academy of Sciences (West Light Program)
文摘Carbonic anhydrase Ⅱ (CAⅡ) is an important enzyme complex with Zn2+,which is involved in many physiological and pathological processes, such as calcification, glaucoma and tumorigenicity. In order to search for novel inhibitors of CAⅡ, inhibition assay of carbonic anhydrase Ⅱ was performed, by which seven natural phenolic compounds, including four phenolics (grifolin, 4-O-methyl-grifolic acid, grifolic acid, and isovanillic acid) and three flavones (eriodictyol, quercetin and puerin A), showed in-hibitory activities against CAⅡ with IC50s in the range of 6.37-71.73 μmol/L. Grifolic acid is the most active one with IC50 of 6.37 μmol/L. These seven phenolic compounds were proved to be novel natural carbonic anhydrase Ⅱ inhibitors, which were obtained in flexible docking study with GOLD 3.0 soft-ware. Results indicated that the aliphatic chain and polar groups of hydroxyl and carboxyl are impor-tant to their inhibitory activities, providing a new insight into study on CA Ⅱ potent inhibitors.
基金the foundations of the National Key Research and Development Program of China [2016YFC0502602]the National Natural Science Foundation of China [No.U1612441-2]Support Plan Projects of Science and Technology Department of Guizhou Province [No.(2021)YB453]。
文摘If the photosynthetic organisms assimilated only CO_(2) in the Archean atmosphere,hydroxide ion in the Archean seawater would not increase.If plants would not consume bicarbonate as a direct substrate during photosynthesis,it is difficult to explain the evolution of Earth's environment.To date,it is generally accepted that photosynthetic O_(2) evolution of plants come from water photolysis.However,it should be debated by evaluating the effect of bicarbonate in photosynthetic O_(2) evolution,analyzing the role of carbonic anhydrase(CA) in photosynthetic O_(2) evolution,and the relationship between thylakoid CA and photosynthetic O_(2) evolution.In the paper,I propose that bicarbonate is directly used as substrate to participate in photosynthetic O_(2) evolution.The rationality of bicarbonate photolysis of plants is discussed from the thermodynamics and evolution of Earth's environment.The isotopic evidence that bicarbonate is not the direct substrate of photosynthetic O_(2) release is reexamined,and the new explanation of bicarbonate photolysis in photosynthetic O_(2) evolution is proposed.
