Objective:To study the therapeutic effect of the Extract of Wuwei Xiaodu Drink on spinal infection and provide the scientific basis for clinical application.Methods:By establishing a rabbit model of spinal infection,t...Objective:To study the therapeutic effect of the Extract of Wuwei Xiaodu Drink on spinal infection and provide the scientific basis for clinical application.Methods:By establishing a rabbit model of spinal infection,this paper observed and analyzed the changes in body mass before and after the intervention and the comparison of inflammation-related factors and blood leukocyte counts among the three groups.Results:There was a significant difference in the changes in body mass of rabbits before and after intervention in the experimental group,control group and blank group(P<0.05);there was no statistically significant difference in calcitoninogen,C-reactive protein and routine blood leukocyte counts between the experimental group and the control group(P>0.05),and there was a statistically significant difference in calcitoninogen,C-reactive protein and routine blood leukocyte counts between the experimental group and the blank group(P<0.05).Conclusion:The Extract of Wuwei Xiaodu Drink can play a protective role by regulating the level of inflammatory factors in blood routine leukocyte count and reducing the inflammatory reaction in the spinal cord injury area.展开更多
[Objectives]To observe the effects of Wuwei Xiaodu Decoction on uterine energy metabolism and serum inflammatory factors in the acute pelvic inflammatory disease(APID)model.[Methods]75 Wistar rats(females)were randoml...[Objectives]To observe the effects of Wuwei Xiaodu Decoction on uterine energy metabolism and serum inflammatory factors in the acute pelvic inflammatory disease(APID)model.[Methods]75 Wistar rats(females)were randomly divided into control group,model group and Wuwei Xiaodu Decoction low,medium and high dose groups(n=15).Except for the control group,the rat APID model was established by right uterine inoculation.On the fifth day after inoculation,the low,medium and high dose groups of Wuwei Xiaodu Decoction were administered at 4,8 and 16 g/kg,and the control group and model group received normal saline.Rats were killed 12 h after nondose administration,blood was collected from the abdominal aorta and measured by ELISA for serum interleukin-6(interleukin-6,IL-6),IL-8,and C-reactive proteins(CRP);the right uterus of rats was tested for high-energy phosphate adenosine phosphate(AMP),adenosine diphosphate(ADP),adenosine triphosphate(ATP)and total adenine nucleotides(TAN)level to evaluate the uterine energy metabolism.[Results]AMP,ADP,ATP and TAN were significantly higher in the Wuwei Xiaodu Decoction of low,medium and high dose than the model group,while the serum IL-6,IL-8 and CRP were significantly lower than the model group,and the difference between the low,medium and high doses(P<0.05).[Conclusions]The Wuwei Xiaodu Decoction can dose-dependent promote uterine energy metabolism and inhibit inflammatory response in APID model rats.展开更多
Objective:To observe the curative effect of Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon on the treatment of HFMD of damp-heat accumulation toxin syndrome and its effects on liver function, myoca...Objective:To observe the curative effect of Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon on the treatment of HFMD of damp-heat accumulation toxin syndrome and its effects on liver function, myocardial enzymes, inflammatory factors and immunoglobulin.Merhods:From June 2018 to November 2018, 74 children with damp-heat accumulation toxin syndrome who were diagnosed with hand, foot and mouth disease have been randomly divided into the observation group (38 cases) and the control group (36 cases).The control group is given conventional Western medicine symptomatic treatment, and sprayed on the oral cavity with IFN- atomized inhalation or IFN- 2b spray. On the basis of the control group, the observation group is given Modified Ganlu Xiaodu Dan, and Kuhuangsan for washing body. 7 d is a course of treatment. The recurrence time of the two groups, the time of rash regression and the efficacy of the children have been observed. The changes of liver function, myocardial zymogram, inflammatory factors and immunoglobulin are compared before and after treatment.Results: The efficacy of the observation group was 92.1% higher than that of the control group (83.3%), and the difference was statistically significant. The time required for the body temperature of the observation group (2.64±0.57) d was lower than that of the control group (3.12±0.62) d, and the time required for the rash to resolve was (4.18±0.69) d, which was lower than that of the control group (5.25±0.73) d. Statistically significant. The levels of ALT, AST, CK-MB, IL-4 and IL-6 in the observation group were lower than those in the control group after treatment, the difference was statistically significant. The serum IgA and IgM levels in the observation group were higher than those in the control group after treatment. Conclusion: Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon for the treatment of HFMD of damp-heat accumulation toxin syndrome have a sound curative effect and it can improve liver function, protect cardiomyocytes, reduce inflammatory factors, and regulate immune function for children.展开更多
Objective: To analyze the potential active components and molecular mechanism of Ganlu Xiaodu MicroPill in the treatment of COVID-19 by network pharmacology and molecular docking. Methods: the active chemical constitu...Objective: To analyze the potential active components and molecular mechanism of Ganlu Xiaodu MicroPill in the treatment of COVID-19 by network pharmacology and molecular docking. Methods: the active chemical constituents and action targets of Ganlu Xiaodu MicroPill were searched by TCMSP and BATMAN-TCM platform, and the key targets were matched with the COVID-19 targets searched by CTD database. The core targets were selected and analyzed by GO and KEGG enrichment analysis by R software to predict the action mechanism of traditional Chinese medicine compound. The key component sets were docked with 3CLpro and ACE2. Results: 138 active components and 53 key targets were obtained. There are 1298 items in GO biological process, 59 items in cell composition, 62 items in molecular function, and 120 items enriched in KEGG pathway. Results: molecular docking showed that the affinity of 20 chemical components such as quercetin, luteolin and kaempferol with ACE2 and 3CLpro was higher than that of ribavirin and chloroquine. Conclusion: Ganlu Xiaodu MicroPill may regulate and interfere with SARS-CoV-2 infection through antivirus, inhibition of inflammatory factors and regulation of immunity, which fully reflects the therapeutic advantages of multi-components, multi-targets and multi-pathways of traditional Chinese medicine.展开更多
Objective:To explore the potential mplecular mechanism of Puji Xiaodu Drink(普济消毒饮,PJX-DD)in the treatment of corona virus disease 2019(COVID-19)based on network pharmacology and molecu-lar docking technology.Meth...Objective:To explore the potential mplecular mechanism of Puji Xiaodu Drink(普济消毒饮,PJX-DD)in the treatment of corona virus disease 2019(COVID-19)based on network pharmacology and molecu-lar docking technology.Methods:The main active compounds of PJXDD were searched by Traditional Chinese Medicine Systems Pharmacology(TCMSP)database and related literature records,and the potential target of PJXDD was found by Swiss Target Prediction;relevant targets of COVID-19 were obtained through Gene Cards database.The PPI network of"drug-compound-target"and target protein interaction was constructed by STRING database and Cytoscape 3.6.1 software.The key targels were analyzed by MCODE plug-in.Finally,the common targets were used for GO enrichment analysis and KEGG pathway analysis by Enrichr.Molecular docking was used to screen the main active components that interact with key targets.Results:202 effective chemical components of PJXDD were screened,and 71 targets were applied to COVID-19.The results of molec-ular docking suggested that naringin and luteolin may be important active compounds of PJXDD against COV-ID-19.They may play a role in the treatment of COVID-19 by parieipating in the signaling pathways of inter-leukin signaling,cytok ine signaling in immune system,and the intrinsic pathway of apoptosis.Conclusion:PJX-DD plays an important role in anti-COVID-19 through multi-component,multi-target and multi-channel action in inflammatory response and immune regulation.展开更多
To explore the effectiveness and safety of a Chinese medicinal decoction Wuwei Xiaodu Drink(WWXDD)in inhibiting chronic osteomyelitis via regulatory T cells signaling.The effective constitutes of WWXDD and osteomyelit...To explore the effectiveness and safety of a Chinese medicinal decoction Wuwei Xiaodu Drink(WWXDD)in inhibiting chronic osteomyelitis via regulatory T cells signaling.The effective constitutes of WWXDD and osteomyelitis related genes were screened.Target proteins were cross-validated using the Venny database.GO function and KEGG pathway analysis were performed for target proteins,while pharmacological network was constructed.The bone properties were analyzed by HE staining and the concentrations of immune factors were measured by ELISA.The expression of CTLA-4 and Foxp3 mRNA and STAT5,p-STAT5,CTLA-4 and Foxp3 protein were detected using Real-time PCR and Western blot,respectively.FACS was used to analyze the percentages of cells.A total of 117 genes overlapped between 785 target genes of the active compounds of WWXDD and 912 osteomyelitis related genes.Inflammation-related genes,including IL-6,TNFα,IL-1βand IL-2 showed high connection degree in the drug-compound-disease-target network.GO function and KEGG pathway analysis revealed that 117 intersection genes mainly enriched in virus infection related pathways,immune related pathways and chemokine signaling pathway.Furthermore,the development of chronic osteomyelitis was suppressed in model rats after treatment with WWXDD.Meanwhile,the concentrations of IL-2 and CD4^(+)CD25^(+)Foxp3 Treg perr centages together with the levels of p-STAT5,CTLA-4 and Foxp3 were also down-regulated.Furthermore,IL-2 and WWXDD drug-containing serum exhibited opposite effects on regulating IL-2,IL-10,TGF-β1,Foxp3,CTLA4 and STAT5.In addition,a STAT5 phosphorylation inhibitor suppressed the expression of Foxp3 and CTLA-4.WWXDD can treat chronic osteomyelitis through suppressing the main regulating factors of Tregs and interfere its immunodepression.Our results bring a new solution for chronic osteomyelitis.展开更多
【目的】评价加味普济消毒颗粒(由金银花、连翘、蒲公英、紫花地丁、荆芥、牛蒡子、皂角刺、赤芍、牡丹皮、芦根等组成)内服联合消肿散结膏(由黄芩、黄连、黄柏、皂角刺等组成)外敷治疗儿童急性扁桃体炎(acute tonsillitis in children,...【目的】评价加味普济消毒颗粒(由金银花、连翘、蒲公英、紫花地丁、荆芥、牛蒡子、皂角刺、赤芍、牡丹皮、芦根等组成)内服联合消肿散结膏(由黄芩、黄连、黄柏、皂角刺等组成)外敷治疗儿童急性扁桃体炎(acute tonsillitis in children,ATC)的临床疗效及对免疫功能与相关炎症指标的影响。【方法】将116例ATC肺胃郁热证患儿随机分为对照组和观察组,每组各58例。对照组给予头孢克肟分散片治疗,观察组给予加味普济消毒颗粒内服联合消肿散结膏外敷治疗,疗程为14 d并随访6个月。观察2组患儿治疗前后中医证候积分、外周血中白细胞计数(WBC)、T淋巴细胞亚群CD3^(+)、CD4^(+)、CD8^(+)和CD4^(+)/CD8^(+)水平及血清肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、C反应蛋白(CRP)水平的变化情况,比较2组患儿的临床疗效和各项临床症状消失时间,同时监测2组患儿的不良反应发生情况和扁桃体炎复发情况。【结果】(1)研究期间,对照组有8例患儿脱落,观察组无脱落病例,最终对照组50例、观察组58例患儿完成全部疗程的治疗。(2)治疗14 d后,观察组的总有效率为98.28%(57/58),对照组为90.00%(45/50);组间比较(秩和检验),观察组的临床疗效明显优于对照组(P<0.05)。(3)治疗后,观察组患儿的咽痛消失时间、脓点消失时间、退热时间及扁桃体恢复正常时间均较对照组明显缩短(P<0.05)。(4)治疗后,2组患儿的主症积分、次症积分和中医证候总积分均较治疗前明显降低(P<0.05),且观察组的降低幅度均明显优于对照组(P<0.05)。(5)治疗后,2组患儿的T淋巴细胞亚群CD3^(+)、CD4^(+)和CD4^(+)/CD8^(+)水平均较治疗前明显升高(P<0.05),CD8^(+)水平均较治疗前明显降低(P<0.05),且观察组对T淋巴细胞亚群CD3^(+)、CD4^(+)和CD4^(+)/CD8^(+)水平的升高幅度及对CD8^(+)水平的降低幅度均明显优于对照组(P<0.05)。(6)治疗后,2组患儿的WBC、TNF-α、IL-1β、IL-6及CRP水平均较治疗前明显降低(P<0.05),且观察组的降低幅度均明显优于对照组(P<0.05)。(7)治疗期间,2组患儿均未出现皮肤过敏及恶心、呕吐等胃肠道不良反应情况,具有较高的安全性。(8)随访6个月,观察组患儿的扁桃体炎复发率为5.17%(3/58),明显低于对照组的24.00%(12/50),组间比较,差异有统计学意义(χ2=8.330,P<0.05)。【结论】加味普济消毒颗粒内服联合消肿散结膏外敷治疗ATC肺胃郁热证患儿疗效显著,可明显缩短病程,改善临床症状,有效降低扁桃体炎复发率,其机制可能与增强机体免疫功能、抑制炎症反应有关。展开更多
基金Shandong Province Traditional Chinese Medicine Science and Technology Project Task Book Number(Project No.:M-2022178)2024 Shandong Provincial Traditional Chinese Medicine Science and Technology Project(Project No.:Z20242407)。
文摘Objective:To study the therapeutic effect of the Extract of Wuwei Xiaodu Drink on spinal infection and provide the scientific basis for clinical application.Methods:By establishing a rabbit model of spinal infection,this paper observed and analyzed the changes in body mass before and after the intervention and the comparison of inflammation-related factors and blood leukocyte counts among the three groups.Results:There was a significant difference in the changes in body mass of rabbits before and after intervention in the experimental group,control group and blank group(P<0.05);there was no statistically significant difference in calcitoninogen,C-reactive protein and routine blood leukocyte counts between the experimental group and the control group(P>0.05),and there was a statistically significant difference in calcitoninogen,C-reactive protein and routine blood leukocyte counts between the experimental group and the blank group(P<0.05).Conclusion:The Extract of Wuwei Xiaodu Drink can play a protective role by regulating the level of inflammatory factors in blood routine leukocyte count and reducing the inflammatory reaction in the spinal cord injury area.
文摘[Objectives]To observe the effects of Wuwei Xiaodu Decoction on uterine energy metabolism and serum inflammatory factors in the acute pelvic inflammatory disease(APID)model.[Methods]75 Wistar rats(females)were randomly divided into control group,model group and Wuwei Xiaodu Decoction low,medium and high dose groups(n=15).Except for the control group,the rat APID model was established by right uterine inoculation.On the fifth day after inoculation,the low,medium and high dose groups of Wuwei Xiaodu Decoction were administered at 4,8 and 16 g/kg,and the control group and model group received normal saline.Rats were killed 12 h after nondose administration,blood was collected from the abdominal aorta and measured by ELISA for serum interleukin-6(interleukin-6,IL-6),IL-8,and C-reactive proteins(CRP);the right uterus of rats was tested for high-energy phosphate adenosine phosphate(AMP),adenosine diphosphate(ADP),adenosine triphosphate(ATP)and total adenine nucleotides(TAN)level to evaluate the uterine energy metabolism.[Results]AMP,ADP,ATP and TAN were significantly higher in the Wuwei Xiaodu Decoction of low,medium and high dose than the model group,while the serum IL-6,IL-8 and CRP were significantly lower than the model group,and the difference between the low,medium and high doses(P<0.05).[Conclusions]The Wuwei Xiaodu Decoction can dose-dependent promote uterine energy metabolism and inhibit inflammatory response in APID model rats.
文摘Objective:To observe the curative effect of Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon on the treatment of HFMD of damp-heat accumulation toxin syndrome and its effects on liver function, myocardial enzymes, inflammatory factors and immunoglobulin.Merhods:From June 2018 to November 2018, 74 children with damp-heat accumulation toxin syndrome who were diagnosed with hand, foot and mouth disease have been randomly divided into the observation group (38 cases) and the control group (36 cases).The control group is given conventional Western medicine symptomatic treatment, and sprayed on the oral cavity with IFN- atomized inhalation or IFN- 2b spray. On the basis of the control group, the observation group is given Modified Ganlu Xiaodu Dan, and Kuhuangsan for washing body. 7 d is a course of treatment. The recurrence time of the two groups, the time of rash regression and the efficacy of the children have been observed. The changes of liver function, myocardial zymogram, inflammatory factors and immunoglobulin are compared before and after treatment.Results: The efficacy of the observation group was 92.1% higher than that of the control group (83.3%), and the difference was statistically significant. The time required for the body temperature of the observation group (2.64±0.57) d was lower than that of the control group (3.12±0.62) d, and the time required for the rash to resolve was (4.18±0.69) d, which was lower than that of the control group (5.25±0.73) d. Statistically significant. The levels of ALT, AST, CK-MB, IL-4 and IL-6 in the observation group were lower than those in the control group after treatment, the difference was statistically significant. The serum IgA and IgM levels in the observation group were higher than those in the control group after treatment. Conclusion: Modified Ganlu Xiaodu Dan and Kuhuang San combined with interferon for the treatment of HFMD of damp-heat accumulation toxin syndrome have a sound curative effect and it can improve liver function, protect cardiomyocytes, reduce inflammatory factors, and regulate immune function for children.
基金Major national science and technology projects-prevention and treatment of major infectious diseases such as AIDS and viral hepatitis(No.2018ZX10303502)National Nature Fund Youth Fund(No.81804063)+2 种基金China postdoctoral Science Foundation Program(No.2019M662500)China postdoctoral Science Foundation Program(No.2019M662500)Key Scientific Research Project of National Clinical Research Base of traditional Chinese Medicine of Henan Provincial Administration of traditional Chinese Medicine(No.2018JDZX1100)
文摘Objective: To analyze the potential active components and molecular mechanism of Ganlu Xiaodu MicroPill in the treatment of COVID-19 by network pharmacology and molecular docking. Methods: the active chemical constituents and action targets of Ganlu Xiaodu MicroPill were searched by TCMSP and BATMAN-TCM platform, and the key targets were matched with the COVID-19 targets searched by CTD database. The core targets were selected and analyzed by GO and KEGG enrichment analysis by R software to predict the action mechanism of traditional Chinese medicine compound. The key component sets were docked with 3CLpro and ACE2. Results: 138 active components and 53 key targets were obtained. There are 1298 items in GO biological process, 59 items in cell composition, 62 items in molecular function, and 120 items enriched in KEGG pathway. Results: molecular docking showed that the affinity of 20 chemical components such as quercetin, luteolin and kaempferol with ACE2 and 3CLpro was higher than that of ribavirin and chloroquine. Conclusion: Ganlu Xiaodu MicroPill may regulate and interfere with SARS-CoV-2 infection through antivirus, inhibition of inflammatory factors and regulation of immunity, which fully reflects the therapeutic advantages of multi-components, multi-targets and multi-pathways of traditional Chinese medicine.
基金We thank for the funding support from the Henan Emergency Tackling Project(201100312300)Zhengzhou Emergency Tackling Project(ZZKJ2020604)。
文摘Objective:To explore the potential mplecular mechanism of Puji Xiaodu Drink(普济消毒饮,PJX-DD)in the treatment of corona virus disease 2019(COVID-19)based on network pharmacology and molecu-lar docking technology.Methods:The main active compounds of PJXDD were searched by Traditional Chinese Medicine Systems Pharmacology(TCMSP)database and related literature records,and the potential target of PJXDD was found by Swiss Target Prediction;relevant targets of COVID-19 were obtained through Gene Cards database.The PPI network of"drug-compound-target"and target protein interaction was constructed by STRING database and Cytoscape 3.6.1 software.The key targels were analyzed by MCODE plug-in.Finally,the common targets were used for GO enrichment analysis and KEGG pathway analysis by Enrichr.Molecular docking was used to screen the main active components that interact with key targets.Results:202 effective chemical components of PJXDD were screened,and 71 targets were applied to COVID-19.The results of molec-ular docking suggested that naringin and luteolin may be important active compounds of PJXDD against COV-ID-19.They may play a role in the treatment of COVID-19 by parieipating in the signaling pathways of inter-leukin signaling,cytok ine signaling in immune system,and the intrinsic pathway of apoptosis.Conclusion:PJX-DD plays an important role in anti-COVID-19 through multi-component,multi-target and multi-channel action in inflammatory response and immune regulation.
基金the National Natural Science Foundation of China(No.81603644)the Natural Science Foundation of Zhejiang Province(No.LY20H270002)+1 种基金Zhejiang TCM Science and Technology Program(No.2020ZQ006)Zhejiang Medical and Health Science and Technology Program(No.2020RC048).
文摘To explore the effectiveness and safety of a Chinese medicinal decoction Wuwei Xiaodu Drink(WWXDD)in inhibiting chronic osteomyelitis via regulatory T cells signaling.The effective constitutes of WWXDD and osteomyelitis related genes were screened.Target proteins were cross-validated using the Venny database.GO function and KEGG pathway analysis were performed for target proteins,while pharmacological network was constructed.The bone properties were analyzed by HE staining and the concentrations of immune factors were measured by ELISA.The expression of CTLA-4 and Foxp3 mRNA and STAT5,p-STAT5,CTLA-4 and Foxp3 protein were detected using Real-time PCR and Western blot,respectively.FACS was used to analyze the percentages of cells.A total of 117 genes overlapped between 785 target genes of the active compounds of WWXDD and 912 osteomyelitis related genes.Inflammation-related genes,including IL-6,TNFα,IL-1βand IL-2 showed high connection degree in the drug-compound-disease-target network.GO function and KEGG pathway analysis revealed that 117 intersection genes mainly enriched in virus infection related pathways,immune related pathways and chemokine signaling pathway.Furthermore,the development of chronic osteomyelitis was suppressed in model rats after treatment with WWXDD.Meanwhile,the concentrations of IL-2 and CD4^(+)CD25^(+)Foxp3 Treg perr centages together with the levels of p-STAT5,CTLA-4 and Foxp3 were also down-regulated.Furthermore,IL-2 and WWXDD drug-containing serum exhibited opposite effects on regulating IL-2,IL-10,TGF-β1,Foxp3,CTLA4 and STAT5.In addition,a STAT5 phosphorylation inhibitor suppressed the expression of Foxp3 and CTLA-4.WWXDD can treat chronic osteomyelitis through suppressing the main regulating factors of Tregs and interfere its immunodepression.Our results bring a new solution for chronic osteomyelitis.
