Pomelo peel is a by-product from pomelo consumption, but it is also rich in a variety of nutrients and functional substances. In order to solve the environmental problems caused by the large amount of discarded pomelo...Pomelo peel is a by-product from pomelo consumption, but it is also rich in a variety of nutrients and functional substances. In order to solve the environmental problems caused by the large amount of discarded pomelo peels every year, in this study, we recycled pomelo peels to make pomelo yellow peel jelly (PYPJ) and pomelo white peel jelly (PWPJ) according to their different characteristics in pomelo peels. And the consumer sensory evaluation (n = 50) was carried out to explore the consumers’ evaluation and acceptance for these two innovative products. The results showed that both jellies received high mean scores for all sensory attributes expect PYPJ’ taste, and the consumer acceptances of PYPJ and PWPJ were 62% and 92%, respectively. These results displayed that there is a need to further improve PYPJ, and PWPJ is a product recognized by consumers. .展开更多
Objective:Exploring the formation and aggregation of human islet amyloid polypeptide(hIAPP)(amylin)fibers is significant for promoting the prevention and treatment of type II diabetes mellitus(T2DM).Flavones in pomelo...Objective:Exploring the formation and aggregation of human islet amyloid polypeptide(hIAPP)(amylin)fibers is significant for promoting the prevention and treatment of type II diabetes mellitus(T2DM).Flavones in pomelo peel have visible biological activity in the anti-diabetes aspect.The present study aimed to investigate the effects of five flavones[naringin(NRG),narirutin(NRR),nobiletin(NOB),sinensetin(SIN),and neohesperidin(NHP)]in pomelo peel on peptide aggregation and explore its possible mechanisms.The cell viability of flavones against peptide aggregation was also evaluated.Methods:The thioflavin T(ThT)assay and transmission electron microscopy(TEM)were used for evaluating the inhibition and disaggregation of flavones on peptide aggregation.The interaction mechanism was analyzed by endogenous fluorescence,molecular dynamics(MD)simulations,ultraviolet spectroscopy(UV)and isothermal titration calorimetry(ITC)experiments.The 3-[4,5-dimethylthiazol-2-y l]-2,5-diphenyl tetrazolium bromide(MTT)and immune assays were performed to characterize the cell viability of flavones against peptide aggregation.Results:The five flavones showed a decrease in fluorescence intensity,fiber number and size under incubation with different molar ratios of hIAPP.The compounds can bind to the aromatic tyrosine(Tyr)residueTyr 37,resulting in the intrinsic fluorescence quenching of the peptides.Five flavones can form hydrogen bonds with hIAPP,which is likely to be based on their phenolic hydroxyl structure.They showed strong binding affinity with peptides.The reaction system of NRG and NRR observed an exothermic reaction,and the others were endothermic reactions.The absorption peaks of the compounds with hIAPP changed and showed hypochromic effects,indicating that there may beπ-πstacking interaction.Flavones noticeably increased the cell viability in the presence of amyloid peptides and reduced the absorption intensity induced by peptide oligomers.Conclusion:A total of five flavones in pomelo peel have inhibitory and depolymerization effects on amyloid fibrils,and can significantly protect cells from the toxic effect of hIAPP and reduce the production of toxic oligomers.展开更多
Bacterial infection and scar formation remain primary challenges in wound healing.To address these issues,we developed a decellularized pomelo peel(DPP)functionalized with an adhesive PVA-TSPBA hydrogel and antibacter...Bacterial infection and scar formation remain primary challenges in wound healing.To address these issues,we developed a decellularized pomelo peel(DPP)functionalized with an adhesive PVA-TSPBA hydrogel and antibacterial gallic acid/copper MOFs.The hybrid wound dressing demonstrates favorable biocompatibility.It does not impede the proliferation of fibroblasts or immune cells and can stimulate fibroblast migration,endothelial angiogenesis,and M2 macrophage polarization.Additionally,the dressing can scavenge reactive oxygen species(ROS)and provide antioxidant effects.Furthermore,DPP+MOF@Gel effectively inhibits the viability of S.aureus and E.coli in vitro and in vivo.The histological observations revealed enhanced granulation tissue formation,re-epithelialization,and angiogenesis in the DPP+MOF@Gel group compared to other groups.The local immune response also shifted from a pro-inflammatory to a pro-regenerative status with DPP+MOF@Gel treatment.The skin incision stitching experiment further exhibits DPP+MOF@Gel could reduce scar formation during wound healing.Taken together,the hybrid DPP+MOF@Gel holds great promise for treating bacteria-infected skin wounds and inhibiting scar formation during wound healing.展开更多
To reveal the influence of the diversity of precursors on the formation of environmental persistent free radicals(EPFRs),pomelo peel(PP)and its physically divided portion,pomelo cuticle(PC),and white fiber(WF)were use...To reveal the influence of the diversity of precursors on the formation of environmental persistent free radicals(EPFRs),pomelo peel(PP)and its physically divided portion,pomelo cuticle(PC),and white fiber(WF)were used as precursors to prepare six hydrochars:PPH-Fe,PCH-Fe,WFH-Fe,PPH,PCH,and WFH with and without Fe(III)addition during hydrothermal carbonization(HTC).PPH-Fe and WFH-Fe had higher EPFRs content(9.11×10^(18)and 8.25×10^(18)spins·g^(−1))compared to PPH and WFH(3.33×10^(18)and 2.96×10^(18)spins·g^(−1)),indicating that iron-doping favored EPFRs formation.However,PCH-Fe had lower EPFRs content(2.78×10^(18)spins·g^(−1))than PCH(7.95×10^(18)spins·g^(−1)),possibly due to excessive iron leading to the consumption of the generated EPFRs.For another reason,the required Fe(III)amount for EPFRs formation might vary among different precursors.PC has a lower concentration of phenolic compounds but 68-97%fatty acids,while WF and PP are rich in cellulose and lignin.In the Fenton-like reaction,oxygen-centered radicals of hydrochar played a significant role in activating H_(2)O_(2)and efficiently degrading bisphenol A(BPA).Mechanisms of reactive oxygen species(ROS)generation in hydrochar/H_(2)O_(2)system were proposed.EPFRs on hydrochar activate H_(2)O_(2)via electron transfer,creating·OH and 1O2,leading to BPA degradation.More importantly,the embedded EPFRs on the hydrochar’s inner surface contributed to the prolonged Fenton-like reactivity of PPH-Fe stored for 45 days.This study demonstrates that by optimizing precursor selection and iron doping,hydrochars can be engineered to maximize their EPFRs content and reactivity,providing a cost-effective solution for the degradation of hazardous pollutants.展开更多
The porous structure in pomelo peel is believed to be responsible for the protection of its fruit from damage during the free falling from a tree.The quantitative understanding of the relationship between the deformat...The porous structure in pomelo peel is believed to be responsible for the protection of its fruit from damage during the free falling from a tree.The quantitative understanding of the relationship between the deformation behavior and the porous structure could pave the way for the design of porous structures for efficient energy absorption.Here,a universal feature of pore distribution in pomelo peels along the radial direction is extracted from three varieties of pomelos,which shows strong correlation to the deformation behavior of the peels under compression.Guided by the porous design found in pomelo peels,porous polyether-ether-ketone(PEEK)cube is additively manufactured and possesses the highest ability to absorb energy during compression as compared to the non-pomelo-inspired geometries,which is further confirmed by the finite element simulation.The nature-optimized porous structure revealed here could guide the design of lightweight and high-energy-dissipating materials/devices.展开更多
In this study, pectin was extracted from grapefruit peels with hydrochloric acid and precipitated with 95% ethanol solution and 5% tartaric acid. The physical and chemical properties of pectin obtained were analyzed, ...In this study, pectin was extracted from grapefruit peels with hydrochloric acid and precipitated with 95% ethanol solution and 5% tartaric acid. The physical and chemical properties of pectin obtained were analyzed, including color, texture, degree of esterification, and water solubility. The effects of peel to sol- vent ratio, pH of the solvent, extraction temperature and extraction duration on pectin extraction rate were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal experiment was performed to screen the optimal extraction conditions. According to the results, the optimal combination of technical parame- ters was A1B3 C1 D3, i. e. , solid to liquid ratio 1：40 （g： ml）, pH 2.0, extraction duration 70 rain, extraction temperature 90 ℃. The average pectin extraction rate was 68.60% ; the color of pectin extracted under the optimal conditions varied from milky white to light yellow; the degree of esterification of pectin extracts was 65. 71% ; the extracted pectin exhibited good water solubility. Key words Pomelo peel; Pectin extraction; Optimum conditions; Esterification degree; Water solubility展开更多
文摘Pomelo peel is a by-product from pomelo consumption, but it is also rich in a variety of nutrients and functional substances. In order to solve the environmental problems caused by the large amount of discarded pomelo peels every year, in this study, we recycled pomelo peels to make pomelo yellow peel jelly (PYPJ) and pomelo white peel jelly (PWPJ) according to their different characteristics in pomelo peels. And the consumer sensory evaluation (n = 50) was carried out to explore the consumers’ evaluation and acceptance for these two innovative products. The results showed that both jellies received high mean scores for all sensory attributes expect PYPJ’ taste, and the consumer acceptances of PYPJ and PWPJ were 62% and 92%, respectively. These results displayed that there is a need to further improve PYPJ, and PWPJ is a product recognized by consumers. .
基金supported by the National Natural Science Foundation of China(No.82174074,81873092)。
文摘Objective:Exploring the formation and aggregation of human islet amyloid polypeptide(hIAPP)(amylin)fibers is significant for promoting the prevention and treatment of type II diabetes mellitus(T2DM).Flavones in pomelo peel have visible biological activity in the anti-diabetes aspect.The present study aimed to investigate the effects of five flavones[naringin(NRG),narirutin(NRR),nobiletin(NOB),sinensetin(SIN),and neohesperidin(NHP)]in pomelo peel on peptide aggregation and explore its possible mechanisms.The cell viability of flavones against peptide aggregation was also evaluated.Methods:The thioflavin T(ThT)assay and transmission electron microscopy(TEM)were used for evaluating the inhibition and disaggregation of flavones on peptide aggregation.The interaction mechanism was analyzed by endogenous fluorescence,molecular dynamics(MD)simulations,ultraviolet spectroscopy(UV)and isothermal titration calorimetry(ITC)experiments.The 3-[4,5-dimethylthiazol-2-y l]-2,5-diphenyl tetrazolium bromide(MTT)and immune assays were performed to characterize the cell viability of flavones against peptide aggregation.Results:The five flavones showed a decrease in fluorescence intensity,fiber number and size under incubation with different molar ratios of hIAPP.The compounds can bind to the aromatic tyrosine(Tyr)residueTyr 37,resulting in the intrinsic fluorescence quenching of the peptides.Five flavones can form hydrogen bonds with hIAPP,which is likely to be based on their phenolic hydroxyl structure.They showed strong binding affinity with peptides.The reaction system of NRG and NRR observed an exothermic reaction,and the others were endothermic reactions.The absorption peaks of the compounds with hIAPP changed and showed hypochromic effects,indicating that there may beπ-πstacking interaction.Flavones noticeably increased the cell viability in the presence of amyloid peptides and reduced the absorption intensity induced by peptide oligomers.Conclusion:A total of five flavones in pomelo peel have inhibitory and depolymerization effects on amyloid fibrils,and can significantly protect cells from the toxic effect of hIAPP and reduce the production of toxic oligomers.
基金financially supported by the National Natural Science Foundation of China(grant no.82102334 to S.Chen,grant no.82272204 to J.Pan,grant no.82360446 to W.Wan)Wenzhou Science and Technology Major Project(grant no.ZY2022026 to S.Chen)+2 种基金Pioneer”and“Leading Goose”R&D Program of Zhejiang(grant no.2023C03084 to J.Pan)Foundation of Health Commission of Jiangxi Province(Grant No.202210603 to W.Wan)the“Thousand Talents Plan”of Jiangxi Province Introduces and Trains Innovative and Entrepreneurial High-level Talents(jxsq2023201027).
文摘Bacterial infection and scar formation remain primary challenges in wound healing.To address these issues,we developed a decellularized pomelo peel(DPP)functionalized with an adhesive PVA-TSPBA hydrogel and antibacterial gallic acid/copper MOFs.The hybrid wound dressing demonstrates favorable biocompatibility.It does not impede the proliferation of fibroblasts or immune cells and can stimulate fibroblast migration,endothelial angiogenesis,and M2 macrophage polarization.Additionally,the dressing can scavenge reactive oxygen species(ROS)and provide antioxidant effects.Furthermore,DPP+MOF@Gel effectively inhibits the viability of S.aureus and E.coli in vitro and in vivo.The histological observations revealed enhanced granulation tissue formation,re-epithelialization,and angiogenesis in the DPP+MOF@Gel group compared to other groups.The local immune response also shifted from a pro-inflammatory to a pro-regenerative status with DPP+MOF@Gel treatment.The skin incision stitching experiment further exhibits DPP+MOF@Gel could reduce scar formation during wound healing.Taken together,the hybrid DPP+MOF@Gel holds great promise for treating bacteria-infected skin wounds and inhibiting scar formation during wound healing.
基金supported by the National Key Research and Development Program of China(No.2020YFC1808103)Science&Technology Commission of Shanghai Municipality(No.22002400500)+1 种基金National Natural Science Foundation of China(20907029 and 21577085)Natural Science Fund Projects of Shanghai Municipal Science and Technology Commission(No.14ZR1415600).
文摘To reveal the influence of the diversity of precursors on the formation of environmental persistent free radicals(EPFRs),pomelo peel(PP)and its physically divided portion,pomelo cuticle(PC),and white fiber(WF)were used as precursors to prepare six hydrochars:PPH-Fe,PCH-Fe,WFH-Fe,PPH,PCH,and WFH with and without Fe(III)addition during hydrothermal carbonization(HTC).PPH-Fe and WFH-Fe had higher EPFRs content(9.11×10^(18)and 8.25×10^(18)spins·g^(−1))compared to PPH and WFH(3.33×10^(18)and 2.96×10^(18)spins·g^(−1)),indicating that iron-doping favored EPFRs formation.However,PCH-Fe had lower EPFRs content(2.78×10^(18)spins·g^(−1))than PCH(7.95×10^(18)spins·g^(−1)),possibly due to excessive iron leading to the consumption of the generated EPFRs.For another reason,the required Fe(III)amount for EPFRs formation might vary among different precursors.PC has a lower concentration of phenolic compounds but 68-97%fatty acids,while WF and PP are rich in cellulose and lignin.In the Fenton-like reaction,oxygen-centered radicals of hydrochar played a significant role in activating H_(2)O_(2)and efficiently degrading bisphenol A(BPA).Mechanisms of reactive oxygen species(ROS)generation in hydrochar/H_(2)O_(2)system were proposed.EPFRs on hydrochar activate H_(2)O_(2)via electron transfer,creating·OH and 1O2,leading to BPA degradation.More importantly,the embedded EPFRs on the hydrochar’s inner surface contributed to the prolonged Fenton-like reactivity of PPH-Fe stored for 45 days.This study demonstrates that by optimizing precursor selection and iron doping,hydrochars can be engineered to maximize their EPFRs content and reactivity,providing a cost-effective solution for the degradation of hazardous pollutants.
基金supported by the National Key R&DProgram of China(2018YFB1105100)National Natural Science Foundation of China(51973165).
文摘The porous structure in pomelo peel is believed to be responsible for the protection of its fruit from damage during the free falling from a tree.The quantitative understanding of the relationship between the deformation behavior and the porous structure could pave the way for the design of porous structures for efficient energy absorption.Here,a universal feature of pore distribution in pomelo peels along the radial direction is extracted from three varieties of pomelos,which shows strong correlation to the deformation behavior of the peels under compression.Guided by the porous design found in pomelo peels,porous polyether-ether-ketone(PEEK)cube is additively manufactured and possesses the highest ability to absorb energy during compression as compared to the non-pomelo-inspired geometries,which is further confirmed by the finite element simulation.The nature-optimized porous structure revealed here could guide the design of lightweight and high-energy-dissipating materials/devices.
基金Supported by Project of Education Department of Jiangxi Province(JXJG-08-4-27)
文摘In this study, pectin was extracted from grapefruit peels with hydrochloric acid and precipitated with 95% ethanol solution and 5% tartaric acid. The physical and chemical properties of pectin obtained were analyzed, including color, texture, degree of esterification, and water solubility. The effects of peel to sol- vent ratio, pH of the solvent, extraction temperature and extraction duration on pectin extraction rate were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal experiment was performed to screen the optimal extraction conditions. According to the results, the optimal combination of technical parame- ters was A1B3 C1 D3, i. e. , solid to liquid ratio 1：40 （g： ml）, pH 2.0, extraction duration 70 rain, extraction temperature 90 ℃. The average pectin extraction rate was 68.60% ; the color of pectin extracted under the optimal conditions varied from milky white to light yellow; the degree of esterification of pectin extracts was 65. 71% ; the extracted pectin exhibited good water solubility. Key words Pomelo peel; Pectin extraction; Optimum conditions; Esterification degree; Water solubility
