Parthenogenesis is a kind of reproductive mode by which the egg develops into embryo without fertilization and grows into plant finally. This article summarizes the advances of inducing parthenogenesis in plants in re...Parthenogenesis is a kind of reproductive mode by which the egg develops into embryo without fertilization and grows into plant finally. This article summarizes the advances of inducing parthenogenesis in plants in relation to induction methods, affecting factors and the studies in embryology. In addition, the application of parthenogenesis in breeding is also discussed.展开更多
Meat and milk production needs to increase ～ 70–80% relative to its current levels for satisfying the human needs in 2050.However,it is impossible to achieve such genetic gain by conventional animal breeding systems...Meat and milk production needs to increase ～ 70–80% relative to its current levels for satisfying the human needs in 2050.However,it is impossible to achieve such genetic gain by conventional animal breeding systems.Based on recent advances with regard to in vitro induction of germ cell from pluripotent stem cells,herein we propose a novel embryo-stem cell breeding system.Distinct from the conventional breeding system in farm animals that involves selecting and mating individuals,the novel breeding system completes breeding cycles from parental to offspring embryos directly by selecting and mating embryos in a dish.In comparison to the conventional dairy breeding scheme,this system can rapidly achieve 30–40 times more genetic gain by significantly shortening generation interval and enhancing selection intensity.However,several major obstacles must be overcome before we can fully use this system in livestock breeding,which include derivation and mantaince of pluripotent stem cells in domestic animals,as well as in vitro induction of primordial germ cells,and subsequent haploid gametes.Thus,we also discuss the potential efforts needed in solving the obstacles for application this novel system,and elaborate on their groundbreaking potential in livestock breeding.This novel system would provide a revolutionary animal breeding system by offering an unprecedented opportunity for meeting the fast-growing meat and milk demand of humans.展开更多
Doubled haploid(DH)technology and synthetic apomixis approaches can considerably shorten breeding cycles and enhance breeding efficiency.Compared with traditional breeding methods,DH technology offers the advantage of...Doubled haploid(DH)technology and synthetic apomixis approaches can considerably shorten breeding cycles and enhance breeding efficiency.Compared with traditional breeding methods,DH technology offers the advantage of rapidly generating inbred lines,while synthetic apomixis can effectively fix hybrid vigor.In this review,we focus on(i)recent advances in identifying and characterizing genes responsible for haploid induction(Hl),(ii)the molecular mechanisms of Hl,(ili)spontaneous haploid genome doubling,and(iv)crop synthetic apomixis.We also discuss the challenges and potential solutions for future crop breeding programs utilizing DH technology and synthetic apomixis.Finally,we provide our perspectives about how to integrate DH and synthetic apomixis for precision breeding and de novo domestication.展开更多
Doubled haploid(DH)technology is an important tool in crop breeding because it can significantly accelerate the breeding process.ZmPLA1/MATL/NLD and ZmDMP are two key genes controlling haploid induction(HI)in maize,ex...Doubled haploid(DH)technology is an important tool in crop breeding because it can significantly accelerate the breeding process.ZmPLA1/MATL/NLD and ZmDMP are two key genes controlling haploid induction(HI)in maize,exhibiting a synergistic effect.However,it is unknown whether knock out of ZmDMP orthologs can stimulate HI in rice.In this study,a ZmPLA1 ortholog(OsPLA1)and two ZmDMP orthologs(OsDMP3 and OsDMP6)were identified in rice.All three genes encode plasma membrane-localized proteins and were highly expressed in mature anthers.Knockout of OsPLA1 in both Minghui 63 and Nipponbare resulted in reduced seed setting rate(SSR)and caused HI.The osdmp3,osdmp6 and the double mutant failed to trigger HI independently,nor increased the haploid induction rate(HIR)when combined with ospla1.Repeated pollinations operations of QX654A with the ospla1 mutant significantly improve SSR,while reducing HIR.RNA-seq profiling of mature ospla1 mutant anthers indicated that a large number of differentially expressed genes(DEGs)were enriched in redox homeostasis and lipid metabolic GO terms,plant hormone signal transduction,and MAPK signaling pathways.These findings provide important insights towards construction of an efficient DH breeding technology and study of the molecular mechanism of HI in rice.展开更多
Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the...Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the combination of different modes,the substrate of strawberry cultivation,the drip irrigation system,the formula of nutrient solution,the selection of varieties,the propagation of seed and seedling,the induction of flower buds,the planting and management of plants,and the green control of diseases and insect pests are introduced,and greenhouse space and solar energy are fully used to maximize the economic and social benefits of strawberry farming combined with tourism.The results will further promote the quality and efficiency of strawberry industry in southern Xinjiang,and provide a reference for strawberry growers.展开更多
文摘Parthenogenesis is a kind of reproductive mode by which the egg develops into embryo without fertilization and grows into plant finally. This article summarizes the advances of inducing parthenogenesis in plants in relation to induction methods, affecting factors and the studies in embryology. In addition, the application of parthenogenesis in breeding is also discussed.
基金supported by Strategy Research on Disruptive Technology in Agriculture(China Academy of Engineering,2017-ZD-10-07)supported by grants from the National Key R&D Program(2017YFD0501901+1 种基金2017YFD0501905)the Earmarked Fund for the Innovative Teams of Beijing Swine Industrialization Research Program.National Waterfowl-industry Technology Research System(CARS-42)
文摘Meat and milk production needs to increase ～ 70–80% relative to its current levels for satisfying the human needs in 2050.However,it is impossible to achieve such genetic gain by conventional animal breeding systems.Based on recent advances with regard to in vitro induction of germ cell from pluripotent stem cells,herein we propose a novel embryo-stem cell breeding system.Distinct from the conventional breeding system in farm animals that involves selecting and mating individuals,the novel breeding system completes breeding cycles from parental to offspring embryos directly by selecting and mating embryos in a dish.In comparison to the conventional dairy breeding scheme,this system can rapidly achieve 30–40 times more genetic gain by significantly shortening generation interval and enhancing selection intensity.However,several major obstacles must be overcome before we can fully use this system in livestock breeding,which include derivation and mantaince of pluripotent stem cells in domestic animals,as well as in vitro induction of primordial germ cells,and subsequent haploid gametes.Thus,we also discuss the potential efforts needed in solving the obstacles for application this novel system,and elaborate on their groundbreaking potential in livestock breeding.This novel system would provide a revolutionary animal breeding system by offering an unprecedented opportunity for meeting the fast-growing meat and milk demand of humans.
基金the National Natural Science Foundation of China(32321005)the Science and Technology Major Program of Hubei Province(2021ABA011)+1 种基金A.R.F.acknowledges the European Union's Horizon 2020 research and innovation programme,project PlantaSYST(SGA-CSA no.739582 under FPA no.664620)the BG05M2OP001-1.003-001-C01 project,financed by the European Regional Development Fund through the BulgarianEuropean Regional Development Fund through the Bulgarian“Science and Education for Smart Growth"Operational Programme。
文摘Doubled haploid(DH)technology and synthetic apomixis approaches can considerably shorten breeding cycles and enhance breeding efficiency.Compared with traditional breeding methods,DH technology offers the advantage of rapidly generating inbred lines,while synthetic apomixis can effectively fix hybrid vigor.In this review,we focus on(i)recent advances in identifying and characterizing genes responsible for haploid induction(Hl),(ii)the molecular mechanisms of Hl,(ili)spontaneous haploid genome doubling,and(iv)crop synthetic apomixis.We also discuss the challenges and potential solutions for future crop breeding programs utilizing DH technology and synthetic apomixis.Finally,we provide our perspectives about how to integrate DH and synthetic apomixis for precision breeding and de novo domestication.
基金This work was supported by the National Key Research and Development Program of China(2022YFD1200800)the China Agriculture Research System(CARS-02-05)+1 种基金Beijing Nova Program(2023067)Yunnan Province Science and Technology Department(202305AF150026).
文摘Doubled haploid(DH)technology is an important tool in crop breeding because it can significantly accelerate the breeding process.ZmPLA1/MATL/NLD and ZmDMP are two key genes controlling haploid induction(HI)in maize,exhibiting a synergistic effect.However,it is unknown whether knock out of ZmDMP orthologs can stimulate HI in rice.In this study,a ZmPLA1 ortholog(OsPLA1)and two ZmDMP orthologs(OsDMP3 and OsDMP6)were identified in rice.All three genes encode plasma membrane-localized proteins and were highly expressed in mature anthers.Knockout of OsPLA1 in both Minghui 63 and Nipponbare resulted in reduced seed setting rate(SSR)and caused HI.The osdmp3,osdmp6 and the double mutant failed to trigger HI independently,nor increased the haploid induction rate(HIR)when combined with ospla1.Repeated pollinations operations of QX654A with the ospla1 mutant significantly improve SSR,while reducing HIR.RNA-seq profiling of mature ospla1 mutant anthers indicated that a large number of differentially expressed genes(DEGs)were enriched in redox homeostasis and lipid metabolic GO terms,plant hormone signal transduction,and MAPK signaling pathways.These findings provide important insights towards construction of an efficient DH breeding technology and study of the molecular mechanism of HI in rice.
基金Supported by Science and Technology Project of the First Division of Xinjiang Production and Construction Corps"Construction and Technology Demonstration of Strawberry Stereoscopic Cultivation Expo Park"(2019NY01)"Three Zones"Science and Technology Talents Project of Xinjiang Production and Construction Corps。
文摘Strawberry is rich in nutrition,which is the only fresh fruit in winter in southern Xinjiang,with good prospect of stereoscopic cultivation.In this paper,the key techniques such as stereoscopic cultivation pattern,the combination of different modes,the substrate of strawberry cultivation,the drip irrigation system,the formula of nutrient solution,the selection of varieties,the propagation of seed and seedling,the induction of flower buds,the planting and management of plants,and the green control of diseases and insect pests are introduced,and greenhouse space and solar energy are fully used to maximize the economic and social benefits of strawberry farming combined with tourism.The results will further promote the quality and efficiency of strawberry industry in southern Xinjiang,and provide a reference for strawberry growers.
