Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and ant...Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. .展开更多
[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw mate...[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.展开更多
Objective: To study the effects of Portulaca oler acea, a Chinese medicinal herb, on insulin resistance in rats with type 2 diabet es mellitus (T2DM). Methods: Experimental model of T2DM was established by injection o...Objective: To study the effects of Portulaca oler acea, a Chinese medicinal herb, on insulin resistance in rats with type 2 diabet es mellitus (T2DM). Methods: Experimental model of T2DM was established by injection of streptozotocin (25mg/kg) and feeding with high calorie forage. The effects o f Portulaca oleracea on oral glucose tolerance, serum levels of insulin, triglyc eride, total cholesterol, high density lipoproteins cholesterol and free f atty acids, and insulin sensitivity index were all observed. Results: Portulaca oleracea could reduce the body weight, improve the impaired glucose tolerance and lipid metabolism, decrease serum free fatty acids, attenuate hyperinsulinemia and elevate insulin sensitivity. Conclusion: Portulaca oleracea could improve insulin resistance i n rats with T2DM, and the mechanism might be related to its actions in improving lipid metabolism and decreasing free fatty acids.展开更多
With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oler...With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.展开更多
To improve understanding of Portulaca pilosa seed germination and lay technical foundation for seedling cultivation,this paper studies the characteristics of dormancy and germination of Portulaca pilosa seed. Research...To improve understanding of Portulaca pilosa seed germination and lay technical foundation for seedling cultivation,this paper studies the characteristics of dormancy and germination of Portulaca pilosa seed. Research indicates that its primary seed dormancy period is about25 d,dormancy can be completely released by storage at room temperature for 30 d; the seed is positively photoblastic seed that can't sprout and can be induced into dormancy in low light conditions; the dormancy-released seed is more sensitive to light when compared with the freshly picked seed,and the germination peak arises 1 d earlier; Portulaca pilosa seed favorites humid conditions,and the higher the soil moisture,the higher the germination rate and germination energy of the seed. The dormancy-released seeds should be used for Portulaca pilosa seedling cultivation,and after the germination is accelerated for 2- 3 d in the light conditions,the seeds are sown on the soil surface,and covered with transparent plastic film to keep moisture and light,which is conducive to the emergence of seeds.展开更多
Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at...Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at all adsorption experiments. Effects of contact time on adsorption capacity of Pb(II) onto Portulaca oleracea leaves were studied. The adsorption capacity increased as the contact time increased and reached equilibrium at one hour. Kinetic models including a first-order, pseudo-second-order and intra-particle diffusion equations were selected to follow the adsorption process. The process follows a pseudo-second-order kinetic and the intra-particle diffusion is the main step to interpret the mechanism of adsorption. Langmuir, Freundlich and Dubinin-Radush Kevich (D-R) isotherm models were applied to describe adsorption equilibrium data. Results proved that the Langmuir isotherm model gave an acceptable fit to the experimental data more than Freundlich isotherm model. Maximum adsorption capacities obtained were 192.3, 333.3 and 434 mg/g at 295, 303 and 310 K, respectively. According to D-R isotherm data, the adsorption process is classified as physical adsorption. Thermodynamically, the adsorption process is non spontaneous, endothermic and random in nature.展开更多
The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a...The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.展开更多
Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the...Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the development of the pharmaceutical industry,the medicinal value of P.oleracea is becoming increasingly prominent.It is commonly used in clinical practice for clearing heat and detoxifying,inhibiting bacteria,preventing and treating diseases such as hypertension,coronary heart disease,and cerebral infarction,indicating its broad application prospects.This study reviews the chemical components and pharmacological activities of P.oleracea in recent decades.展开更多
Background:Portulaca oleracea L.,a traditional medicine with bioactive compounds like flavonoids and omega-3 fatty acids,has gained interest in its neuroendocrine and toxicological effects.Objectives:The aim of this r...Background:Portulaca oleracea L.,a traditional medicine with bioactive compounds like flavonoids and omega-3 fatty acids,has gained interest in its neuroendocrine and toxicological effects.Objectives:The aim of this review is to provide a comprehensive summary of the current knowledge on the neuroendocrine and toxicological activities of Portulaca oleracea.This review also highlights the potential mechanisms of action of these activities.Methods:A literature search was conducted using various databases such as PubMed,ScienceDirect,and Google Scholar.Keywords such as’Portulaca oleracea’,’purslane’,’neuroendocrine activity’,and’toxicological activity’were used to identify relevant articles.Only studies published in English were included in this review.Results:Portulaca oleracea has been found to improve testicular and cognitive function as well as memory in animal models of Alzheimer’s disease and cerebral ischemia due to its antioxidant,anti-inflammatory properties,anti-anxiety and anti-depressant effects.On the other hand,Portulaca oleracea has also been reported to possess toxicological activities.Studies have shown that high doses of Portulaca oleracea extract can cause liver and kidney damage in animal models.The toxic effects of Portulaca oleracea are thought to be due to the presence of oxalates and nitrates in the plant.However,these toxic effects have not been observed in human studies,where Portulaca oleracea was found to be safe for consumption.Conclusion:In conclusion,Portulaca oleracea,a medicinal plant with potential health benefits,has neuroendocrine effects like neuroprotective,anxiolytic,and anti-depressant properties.However,high doses should be used cautiously due to potential toxicological effects.展开更多
In the present study, we aimed to detect and quantify cyclo-dopa amides in Portulaca oleracea L. Together with four known ones （oleracein A-D）, we further identified eight new cyclo-dopa amides, named oleracein H-O,...In the present study, we aimed to detect and quantify cyclo-dopa amides in Portulaca oleracea L. Together with four known ones （oleracein A-D）, we further identified eight new cyclo-dopa amides, named oleracein H-O, using an HPLC-DAD combined with an improved HPLC-ESI-MS/MS method. A standardized HPLC fingerprint of cyclo-dopa amides was generated for the first time through analyzing 11 batches ofP. oleracea. Simultaneously, the contents of two major amides, oleracein A （OA） and oleracein B （OB）, and the total amides （TID） were determined. Our results showed that the contents of OA and OB of P. oleracea ranged from 35.00 to 151.93 mg/kg and from 40.00 to 150.44 mg/kg, respectively. Moreover, TID ranged from 314.16 to 928.60 mg/kg （calculated by OA）, or from 475.83 to 1393.00 mg/kg （calculated by OB）. Taken together, our newly develooed method could be used for the aualitv assessment and oualitv control of this herb.展开更多
Portulaca oleracea, a plant species used as medicine and food, is widely spread in most areas of China. It is also a popular food in some Europe and Mediterranean countries. Purslane contains a variety of chemical con...Portulaca oleracea, a plant species used as medicine and food, is widely spread in most areas of China. It is also a popular food in some Europe and Mediterranean countries. Purslane contains a variety of chemical constituents with pharmacological activities, such as antitumor, hypoglycemic, antioxidant, antibacterial,anti-inflammatory activities. It is also full of plenty of nutrients serving as a diet food or healthy food.Here we reviewed purslane in the following aspects: botanical resources, ethnopharmacological function,chemical constituents, pharmacology and pharmacokinetics, safety evaluation and toxicity, and clinical applications.展开更多
In the present study, we aimed to intensively study the chemical constituents, especially organic acids from a medicinal plant Portulaca oleracea L., and screen their anti-inflammatory and quinone reductase （QR, a ph...In the present study, we aimed to intensively study the chemical constituents, especially organic acids from a medicinal plant Portulaca oleracea L., and screen their anti-inflammatory and quinone reductase （QR, a phase II detoxyfication enzyme） inductive activity. A total of 20 compounds were isolated and identified based on spectroscopic methods, as succinic acid （1）, mono-methyl succinate （2）, L-malic acid （3）, L-l-methyl malate （4）, L-4-methyl malate （5）, L-dimethyl malate （6）, L-6-ethyl citrate （7）, L-1-methyl citrate （8）, L-1,5-dimethyl citrate （9）, 4-hydroxy-5-methylfuran-3-carboxylic acid （10）, 5-hydroxymethyl-furoic acid （11）, stearic acid （12）, L-pyroglutamic acid （13）, cyclo-（tyrosine-leucine） （14）, L-isoleucine （15）, （-）-dehydrovomifoliol （16）, （-）-epiloliolide （17）, 3,4-dihydroxyphenylethanol （18）, succinimide （19）, and uracil （20）. Among them, 14 compounds （2, 4-8, 10, 11, 13-18） were isolated from P. oleracea for the first time. Compotmd 18 （12.5 μM） exhibited potent anti-inflammatory effect in lipopolysaccharide （LPS）-induced macrophage cells （RAW264.7） by reducing NO production, and it also increased QR activity in Hepa lclc7 cells. Compound 16 （50 μM） showed weak QR inductive activity. None of other compounds showed anti-inflammatory or QR inductive activities.展开更多
Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most po...Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease.展开更多
Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The ...Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid–liquid extraction.The number of new blood vessels and VEGF-A expression level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in vascularization.The iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls’staining to determine whether POL extracts can inhibit the iron accumulation.Besides,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively evaluated.Results:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P<0.05).In vivo,administration of P and PD impacted vascularization and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P<0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P<0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory infiltration.Our results suggest that that POL extract is beneficial to repair injured muscle after ischemia–reperfusion,highlighting the potential of POL in the DTPI treatment.展开更多
OBJECTIVE: To find scientific reasons for adverse events reported in Traditional Iranian Medicine (TIM) following administration of aqueous extract of Herba Portulacae Oleraceae seed including itching and tingling of ...OBJECTIVE: To find scientific reasons for adverse events reported in Traditional Iranian Medicine (TIM) following administration of aqueous extract of Herba Portulacae Oleraceae seed including itching and tingling of whole body, tachycardia, anxiety, dyspnea and severe nausea. METHODS: Electronic databases including PubMed, Scopus, and Web of Science were searched up to April 2013 to find papers focused on phytochemistry and biological activities of this plant. RESULTS: Among chemical constituents present in Herba Portulacae oleraceae, catecholamines, adenosine and niacin can cause adverse events similar to those reported inTIM. CONCLUSION: Because of the short duration of action of adenosine, catecholamines and niacin seems to be the major role in appearance of ad-verse events reported in TIM for Herba Portulacae Oleraceae seed. Mechanisms with consideration of receptor types and pharmacokinetics of catecholamine and niacin are warranted to confirm this hypothesis.展开更多
文摘Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. .
文摘[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.
基金Financially supported by Education Ministry of China (Gr ant No. 1998-679)
文摘Objective: To study the effects of Portulaca oler acea, a Chinese medicinal herb, on insulin resistance in rats with type 2 diabet es mellitus (T2DM). Methods: Experimental model of T2DM was established by injection of streptozotocin (25mg/kg) and feeding with high calorie forage. The effects o f Portulaca oleracea on oral glucose tolerance, serum levels of insulin, triglyc eride, total cholesterol, high density lipoproteins cholesterol and free f atty acids, and insulin sensitivity index were all observed. Results: Portulaca oleracea could reduce the body weight, improve the impaired glucose tolerance and lipid metabolism, decrease serum free fatty acids, attenuate hyperinsulinemia and elevate insulin sensitivity. Conclusion: Portulaca oleracea could improve insulin resistance i n rats with T2DM, and the mechanism might be related to its actions in improving lipid metabolism and decreasing free fatty acids.
文摘With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.
基金Supported by Hainan Spark Industrial Zone Project(HNXH201504)
文摘To improve understanding of Portulaca pilosa seed germination and lay technical foundation for seedling cultivation,this paper studies the characteristics of dormancy and germination of Portulaca pilosa seed. Research indicates that its primary seed dormancy period is about25 d,dormancy can be completely released by storage at room temperature for 30 d; the seed is positively photoblastic seed that can't sprout and can be induced into dormancy in low light conditions; the dormancy-released seed is more sensitive to light when compared with the freshly picked seed,and the germination peak arises 1 d earlier; Portulaca pilosa seed favorites humid conditions,and the higher the soil moisture,the higher the germination rate and germination energy of the seed. The dormancy-released seeds should be used for Portulaca pilosa seedling cultivation,and after the germination is accelerated for 2- 3 d in the light conditions,the seeds are sown on the soil surface,and covered with transparent plastic film to keep moisture and light,which is conducive to the emergence of seeds.
文摘Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at all adsorption experiments. Effects of contact time on adsorption capacity of Pb(II) onto Portulaca oleracea leaves were studied. The adsorption capacity increased as the contact time increased and reached equilibrium at one hour. Kinetic models including a first-order, pseudo-second-order and intra-particle diffusion equations were selected to follow the adsorption process. The process follows a pseudo-second-order kinetic and the intra-particle diffusion is the main step to interpret the mechanism of adsorption. Langmuir, Freundlich and Dubinin-Radush Kevich (D-R) isotherm models were applied to describe adsorption equilibrium data. Results proved that the Langmuir isotherm model gave an acceptable fit to the experimental data more than Freundlich isotherm model. Maximum adsorption capacities obtained were 192.3, 333.3 and 434 mg/g at 295, 303 and 310 K, respectively. According to D-R isotherm data, the adsorption process is classified as physical adsorption. Thermodynamically, the adsorption process is non spontaneous, endothermic and random in nature.
文摘The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.
基金This work was financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the development of the pharmaceutical industry,the medicinal value of P.oleracea is becoming increasingly prominent.It is commonly used in clinical practice for clearing heat and detoxifying,inhibiting bacteria,preventing and treating diseases such as hypertension,coronary heart disease,and cerebral infarction,indicating its broad application prospects.This study reviews the chemical components and pharmacological activities of P.oleracea in recent decades.
文摘Background:Portulaca oleracea L.,a traditional medicine with bioactive compounds like flavonoids and omega-3 fatty acids,has gained interest in its neuroendocrine and toxicological effects.Objectives:The aim of this review is to provide a comprehensive summary of the current knowledge on the neuroendocrine and toxicological activities of Portulaca oleracea.This review also highlights the potential mechanisms of action of these activities.Methods:A literature search was conducted using various databases such as PubMed,ScienceDirect,and Google Scholar.Keywords such as’Portulaca oleracea’,’purslane’,’neuroendocrine activity’,and’toxicological activity’were used to identify relevant articles.Only studies published in English were included in this review.Results:Portulaca oleracea has been found to improve testicular and cognitive function as well as memory in animal models of Alzheimer’s disease and cerebral ischemia due to its antioxidant,anti-inflammatory properties,anti-anxiety and anti-depressant effects.On the other hand,Portulaca oleracea has also been reported to possess toxicological activities.Studies have shown that high doses of Portulaca oleracea extract can cause liver and kidney damage in animal models.The toxic effects of Portulaca oleracea are thought to be due to the presence of oxalates and nitrates in the plant.However,these toxic effects have not been observed in human studies,where Portulaca oleracea was found to be safe for consumption.Conclusion:In conclusion,Portulaca oleracea,a medicinal plant with potential health benefits,has neuroendocrine effects like neuroprotective,anxiolytic,and anti-depressant properties.However,high doses should be used cautiously due to potential toxicological effects.
基金National Natural Science Foundation of China(Grant No.81073005)Shandong Province Young and Middle-Aged Scientists Research Awards Fund(Grant No.BS2010YY032)+1 种基金Scientific Research Foundation for Returned Overseas Scholars,Ministry of Education of China(Grant No.42)Innovation Project of Shandong University(Grant No.2012TS102)
文摘In the present study, we aimed to detect and quantify cyclo-dopa amides in Portulaca oleracea L. Together with four known ones （oleracein A-D）, we further identified eight new cyclo-dopa amides, named oleracein H-O, using an HPLC-DAD combined with an improved HPLC-ESI-MS/MS method. A standardized HPLC fingerprint of cyclo-dopa amides was generated for the first time through analyzing 11 batches ofP. oleracea. Simultaneously, the contents of two major amides, oleracein A （OA） and oleracein B （OB）, and the total amides （TID） were determined. Our results showed that the contents of OA and OB of P. oleracea ranged from 35.00 to 151.93 mg/kg and from 40.00 to 150.44 mg/kg, respectively. Moreover, TID ranged from 314.16 to 928.60 mg/kg （calculated by OA）, or from 475.83 to 1393.00 mg/kg （calculated by OB）. Taken together, our newly develooed method could be used for the aualitv assessment and oualitv control of this herb.
基金supported by National Standardization Project of Traditional Chinese Medicine (No. ZYBZH-C-JL-25)
文摘Portulaca oleracea, a plant species used as medicine and food, is widely spread in most areas of China. It is also a popular food in some Europe and Mediterranean countries. Purslane contains a variety of chemical constituents with pharmacological activities, such as antitumor, hypoglycemic, antioxidant, antibacterial,anti-inflammatory activities. It is also full of plenty of nutrients serving as a diet food or healthy food.Here we reviewed purslane in the following aspects: botanical resources, ethnopharmacological function,chemical constituents, pharmacology and pharmacokinetics, safety evaluation and toxicity, and clinical applications.
基金National Natural Science Foundation of China(Grant No.81073005)Science and Technology Development Program of Shandong Province(Grant No.2014GSF119007)+2 种基金Major Project of Science and Technology of Shandong Province(Grant No.2015ZDJS04001)Young Scholars Program of Shandong University(Grant No.YSPSDU2015WLJH50)China-Australia Centre for Health Sciences Research(2015)
文摘In the present study, we aimed to intensively study the chemical constituents, especially organic acids from a medicinal plant Portulaca oleracea L., and screen their anti-inflammatory and quinone reductase （QR, a phase II detoxyfication enzyme） inductive activity. A total of 20 compounds were isolated and identified based on spectroscopic methods, as succinic acid （1）, mono-methyl succinate （2）, L-malic acid （3）, L-l-methyl malate （4）, L-4-methyl malate （5）, L-dimethyl malate （6）, L-6-ethyl citrate （7）, L-1-methyl citrate （8）, L-1,5-dimethyl citrate （9）, 4-hydroxy-5-methylfuran-3-carboxylic acid （10）, 5-hydroxymethyl-furoic acid （11）, stearic acid （12）, L-pyroglutamic acid （13）, cyclo-（tyrosine-leucine） （14）, L-isoleucine （15）, （-）-dehydrovomifoliol （16）, （-）-epiloliolide （17）, 3,4-dihydroxyphenylethanol （18）, succinimide （19）, and uracil （20）. Among them, 14 compounds （2, 4-8, 10, 11, 13-18） were isolated from P. oleracea for the first time. Compotmd 18 （12.5 μM） exhibited potent anti-inflammatory effect in lipopolysaccharide （LPS）-induced macrophage cells （RAW264.7） by reducing NO production, and it also increased QR activity in Hepa lclc7 cells. Compound 16 （50 μM） showed weak QR inductive activity. None of other compounds showed anti-inflammatory or QR inductive activities.
基金supported by Independent Research Projects for young teachers of Minzu University of China [No. 2021NQPY90]。
文摘Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease.
基金supported by the Youth Program of the National Natural Science Foundation of China[grant No.8170,1838]。
文摘Objective:To investigate the role of Portulaca oleracea(POL)in promoting revascularization and reepithelization as well as inhibiting iron aggregation and inflammation of deep tissue pressure injury(DTPI).Methods:The hydroalcoholic extract of POL(P)and aqueous phase fraction of POL(PD)were prepared based on maceration and liquid–liquid extraction.The number of new blood vessels and VEGF-A expression level were assessed using H&E stain and Western blot on injured muscle to examine the role of POL different extracts in vascularization.The iron distribution and total elemental iron of injured muscle were detected using laser ablation inductively coupled plasma mass spectrometry(ICP-MS)and Perls’staining to determine whether POL extracts can inhibit the iron accumulation.Besides,the ability of POL extracts to promote wound healing by combining re-epithelization time,inflammation degree and collagen deposition area were comprehensively evaluated.Results:In vitro,we observed a significant increase in HUVEC cell viability,migration rate and the number of the tube after P and PD treatment(P<0.05).In vivo,administration of P and PD impacted vascularization and iron accumulation on injured tissue,evident from more new blood vessels,higher expression of VEGF-A and decreased muscle iron concentration of treatment groups compared with no-treatment groups(P<0.05).Besides,shorter re-epithelization time,reduced inflammatory infiltration and distinct collagen deposition were associated with administration of P and PD(P<0.05).Conclusion:POL extract administration groups have high-quality wound healing,which is associated with increased new blood vessels,collagen deposition and re-epithelization,along with decreased iron accumulation and inflammatory infiltration.Our results suggest that that POL extract is beneficial to repair injured muscle after ischemia–reperfusion,highlighting the potential of POL in the DTPI treatment.
文摘OBJECTIVE: To find scientific reasons for adverse events reported in Traditional Iranian Medicine (TIM) following administration of aqueous extract of Herba Portulacae Oleraceae seed including itching and tingling of whole body, tachycardia, anxiety, dyspnea and severe nausea. METHODS: Electronic databases including PubMed, Scopus, and Web of Science were searched up to April 2013 to find papers focused on phytochemistry and biological activities of this plant. RESULTS: Among chemical constituents present in Herba Portulacae oleraceae, catecholamines, adenosine and niacin can cause adverse events similar to those reported inTIM. CONCLUSION: Because of the short duration of action of adenosine, catecholamines and niacin seems to be the major role in appearance of ad-verse events reported in TIM for Herba Portulacae Oleraceae seed. Mechanisms with consideration of receptor types and pharmacokinetics of catecholamine and niacin are warranted to confirm this hypothesis.
