Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological damage.Hypoxic preconditioning has been shown to impr...Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological damage.Hypoxic preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are unknown.To this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB pathways.Subsequently,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by asphyxia.The results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these effects.To conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.展开更多
Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational levels.tsRNAs are not on...Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational levels.tsRNAs are not only degradation fragments but also have biological functions,including those in immune inflammation,metabolic disorders,and cell death.tsRNA dysregulation is closely associated with multiple diseases,including various cancers and acute pancreatitis(AP).AP is a common gastrointestinal disease,and its incidence increases annually.AP development is associated with tsRNAs,which regulate cell injury and induce inflammation,especially pyroptosis and ferroptosis.Notably,serum tRF36 has the potential to serve as a non-invasive diagnostic biomarker and leads to pancreatic acinar cell ferroptosis causing inflammation to promote AP.We show the characteristics of tsRNAs and their diagnostic value and function in AP,and discuss the potential opportunities and challenges of using tsRNAs in clinical applications and research.展开更多
Water contamination in areas of formal and informal polymetallic mining is a growing concern in the Peruvian highlands. At the same time, there are agricultural activities that use contaminated water from these rivers...Water contamination in areas of formal and informal polymetallic mining is a growing concern in the Peruvian highlands. At the same time, there are agricultural activities that use contaminated water from these rivers for irrigation. This could contaminate the agricultural products grown on these lands, such as potatoes. It is, therefore, necessary and a priority to determine the adsorption characteristics of these derivatives and to be able to use these natural adsorbents to decontaminate water contaminated with heavy metals.展开更多
Autism spectrum disorder(ASD)is a complex neurodevelopmental disorder affecting over 2%of the global population,marked by social communication deficits and repetitive behaviors.Kabatas et al explored the efficacy and ...Autism spectrum disorder(ASD)is a complex neurodevelopmental disorder affecting over 2%of the global population,marked by social communication deficits and repetitive behaviors.Kabatas et al explored the efficacy and safety of Wharton’s jelly-derived mesenchymal stem cell(WJ-MSC)therapy in a 4-year-old child with ASD.Using the childhood autism rating scale and Denver II develop-mental screening test,significant improvements were seen after six WJ-MSC sessions,with no adverse events over 2 years.Despite promising results,the study’s single-case design limits generalizability.Larger,multi-center trials are needed to validate the findings and assess long-term effects of WJ-MSC therapy in ASD.展开更多
Magnesium hydride(MgH_(2))is an important material for hydrogen(H_(2))storage and transportation owing to its high capacity and reversibility.However,its intrinsic properties have considerably limited its industrial a...Magnesium hydride(MgH_(2))is an important material for hydrogen(H_(2))storage and transportation owing to its high capacity and reversibility.However,its intrinsic properties have considerably limited its industrial application.In this study,the NiFe-800 catalyst as metal-organic framework(MOF)derivative was first utilized to promote the intrinsic properties of MgH_(2).Compared to pure MgH_(2),which releases1.24 wt%H_(2)in 60 min at 275℃,the MgH_(2)-10 NiFe-800 composite releases 5.85 wt%H_(2)in the same time.Even at a lower temperature of 250℃,the MgH_(2)-10 NiFe-800 composite releases 3.57 wt%H_(2),surpassing the performance of pure MgH_(2)at 275℃.Correspondingly,while pure MgH_(2)absorbs 2.08 wt%H_(2)in60 min at 125℃,the MgH_(2)-10 NiFe-800 composite absorbs 5.35 wt%H_(2)in just 1 min,Remarkably,the MgH_(2)-10 NiFe-800 composite absorbs 2.27 wt%H_(2)in 60 min at 50℃and 4.64 wt%H_(2)at 75℃.This indicates that MgH_(2)-10 NiFe-800 exhibits optimum performance with excellent kinetics at low temperatures.Furthermore,the capacity of the MgH_(2)-10 NiFe-800 composite remains largely stable after 10cycles.Moreover,the Mg_(2)Ni/Mg_(2)NiH_(4)acts as a"hydrogen pump",providing effective diffusion channels that enhance the kinetic process of the composite during cycling.Additionally,Fe0facilitates electron transfer and creates hydrogen diffusion channels and catalytic sites.Finally,carbon(C)effectively prevents particle agglomeration and maintains the cyclic stability of the composites.Consequently,the synergistic effects of Mg_(2)Ni/Mg_(2)NiH_(4),Fe^(0),and C considerably improve the kinetic properties and cycling stability of MgH_(2).This work offers an effective and valuable approach to improving the hydrogen storage efficiency in the commercial application of MgH_(2).展开更多
Goji berry is a famous edible and medicinal substance around the world.In this research,15 phenylpropionyl phenylethylamine derivatives(1-15),in cluding one new compound(1),were separated and identified from g oji ber...Goji berry is a famous edible and medicinal substance around the world.In this research,15 phenylpropionyl phenylethylamine derivatives(1-15),in cluding one new compound(1),were separated and identified from g oji berry.All isolates were elucidated via ex tensive nuclear magnetic resonance spectral analyses and chemical techniques.Six known isolates were first obtained from Lycium genus.Six isolates were effectively split in to double chromatographic peaks accompanied by the basically identical areas,indicating they belong to racemates.The oxygen radical absorbance capacity(ORAC)experiment indicated that all isolates displayed a capacity of scavenging free radicals,and most of them exhibited higher ORAC than epigallocatechingallate.In ethanol/palmitic acid-established in vitro hepatocyte injury model,four phenylpropionyl phenylethylamine derivatives(1,2,9,and 15)significantly alleviated hepatocyte injury,among which compound 1 exerted the strongest protective activity.Notably,the hepatoprotective effect of compound 1 was further confirmed in ethanol-established liver damage mouse model,reflected by the reduction of lipid accumulation and the attenuation of pathological alteration.Combined with the in vitro results,the in vivo observations suggested that compound 1 suppressed cell apoptosis and the outburst of inflammation.Our findings provided first-hand evidence proving that goji berry-derived phenylpropionyl phenylethylamine derivatives hold a potential in treating alcoholic liver disease.展开更多
Pulmonary fibrosis significantly contributes to the pathogenesis of acute respiratory distress syndrome(ARDS),markedly increasing patient mortality.Despite the established anti-fibrotic effects of mesenchymal stem cel...Pulmonary fibrosis significantly contributes to the pathogenesis of acute respiratory distress syndrome(ARDS),markedly increasing patient mortality.Despite the established anti-fibrotic effects of mesenchymal stem cells(MSCs),numerous challenges hinder their clinical application.A recent study demon-strated that microvesicles(MVs)from MSCs(MSC-MVs)could attenuate ARDS-related pulmonary fibrosis and enhance lung function via hepatocyte growth factor mRNA transcription.This discovery presents a promising strategy for managing ARDS-associated pulmonary fibrosis.This article initially examines the safety and efficacy of MSCs from both basic science and clinical perspectives,subsequently exploring the potential and obstacles of employing MSC-MVs as a novel therapeutic approach.Additionally,it provides perspectives on future research into the application of MSC-MVs in ARDS-associated pulmonary fi-brosis.展开更多
BACKGROUND Pulmonary fibrosis is one of the main reasons for the high mortality rate among acute respiratory distress syndrome(ARDS)patients.Mesenchymal stromal cell-derived microvesicles(MSC-MVs)have been shown to ex...BACKGROUND Pulmonary fibrosis is one of the main reasons for the high mortality rate among acute respiratory distress syndrome(ARDS)patients.Mesenchymal stromal cell-derived microvesicles(MSC-MVs)have been shown to exert antifibrotic effects in lung diseases.AIM To investigate the effects and mechanisms of MSC-MVs on pulmonary fibrosis in ARDS mouse models.METHODS MSC-MVs with low hepatocyte growth factor(HGF)expression(siHGF-MSC-MVs)were obtained via lentivirus transfection and used to establish the ARDS pulmonary fibrosis mouse model.Following intubation,respiratory mechanics-related indicators were measured via an experimental small animal lung function tester.Homing of MSC-MVs in lung tissues was investigated by near-infrared live imaging.Immunohistochemical,western blotting,ELISA and other methods were used to detect expression of pulmonary fibrosis-related proteins and to compare effects on pulmonary fibrosis and fibrosis-related indicators.RESULTS The MSC-MVs gradually migrated and homed to damaged lung tissues in the ARDS model mice.Treatment with MSC-MVs significantly reduced lung injury and pulmonary fibrosis scores.However,low expression of HGF(siHGF-MSC-MVs)significantly inhibited the effects of MSC-MVs(P<0.05).Compared with the ARDS pulmonary fibrosis group,the MSC-MVs group exhibited suppressed expression of type I collagen antigen,type III collagen antigen,and the proteins transforming growth factor-βandα-smooth muscle actin,whereas the siHGF-MVs group exhibited significantly increased expression of these proteins.In addition,pulmonary compliance and the pressure of oxygen/oxygen inhalation ratio were significantly lower in the MSC-MVs group,and the effects of the MSC-MVs were significantly inhibited by low HGF expression(all P<0.05).CONCLUSION MSC-MVs improved lung ventilation functions and inhibited pulmonary fibrosis in ARDS mice partly via HGF mRNA transfer.展开更多
Broadband electromagnetic(EM)wave absorption materials play an important role in military stealth and health protection.Herein,metal–organic frameworks(MOFs)-derived magnetic-carbon CoNiM@C(M=Cu,Zn,Fe,Mn)microspheres...Broadband electromagnetic(EM)wave absorption materials play an important role in military stealth and health protection.Herein,metal–organic frameworks(MOFs)-derived magnetic-carbon CoNiM@C(M=Cu,Zn,Fe,Mn)microspheres are fabricated,which exhibit flower-like nano–microstructure with tunable EM response capacity.Based on the MOFs-derived CoNi@C microsphere,the adjacent third element is introduced into magnetic CoNi alloy to enhance EM wave absorption performance.In term of broadband absorption,the order of efficient absorption bandwidth(EAB)value is Mn>Fe=Zn>Cu in the CoNiM@C microspheres.Therefore,MOFs-derived flower-like CoNiMn@C microspheres hold outstanding broadband absorption and the EAB can reach up to 5.8 GHz(covering 12.2–18 GHz at 2.0 mm thickness).Besides,off-axis electron holography and computational simulations are applied to elucidate the inherent dielectric dissipation and magnetic loss.Rich heterointerfaces in CoNiMn@C promote the aggregation of the negative/positive charges at the contacting region,forming interfacial polarization.The graphitized carbon layer catalyzed by the magnetic CoNiMn core offered the electron mobility path,boosting the conductive loss.Equally importantly,magnetic coupling is observed in the CoNiMn@C to strengthen the magnetic responding behaviors.This study provides a new guide to build broadband EM absorption by regulating the ternary magnetic alloy.展开更多
BACKGROUND Stromal cell derived factor-1(SDF-1)plays a pivotal role in the recruitment of stem cells to injured livers.However,the changes of SDF-l in patients with hepatitis B virus(HBV)-related acute-on-chronic live...BACKGROUND Stromal cell derived factor-1(SDF-1)plays a pivotal role in the recruitment of stem cells to injured livers.However,the changes of SDF-l in patients with hepatitis B virus(HBV)-related acute-on-chronic liver failure(ACLF)have yet to be elucidated.AIM To study the SDF-1 changes in patients with HBV-related ACLF.METHODS 30 patients with HBV-related ACLF,27 patients with chronic hepatitis B and 20 healthy individuals are involved in our study.The SDF-l mRNA expression in liver tissue was detected by quantitative real-time polymerase chain reaction.Immunohistochemical staining was performed to illustrate the expression of SDFl,CXC receptor 4(CXCR4)and Ki67.The serum SDF-l concentrations were also detected by enzyme-linked immunosorbent assays.RESULTS The expression of SDF-1 mRNA from ACLF patients was remarkably higher than that from other patients(both P<0.05).The expression of SDF-l,CXCR4 and Ki67 from ACLF were the highest among the three groups(all P<0.01).The serum SDF-l levels in ACLF patients were significantly lower than that in other patients(both P<0.01).Moreover,in ACLF patients,the serum SDF-1 Levels were positively correlated with serum total bilirubin and international normalized ratio.In addition,the serum SDF-l levels in survival were significantly lower compared with the non-survivals(P<0.05).The area under the curve for the serum SDF-1 level in predicting 28-d mortality was 0.722(P<0.05).CONCLUSION This study provides the SDF-1 changes in patients with HBV-related ACLF.The SDF-1 Level at admission may serve as a promising prognostic marker for predicting short-term prognosis.展开更多
Plant growth regulators(PGRs)are chemical substances that imitate the functions of phytohormones to enhance the crop yield and the harvest process.Phenylurea-derived plant growth regulators are known for their excelle...Plant growth regulators(PGRs)are chemical substances that imitate the functions of phytohormones to enhance the crop yield and the harvest process.Phenylurea-derived plant growth regulators are known for their excellent efficacy in promoting fruit growth,particularly in kiwifruit,grapes,and melons.Phenylurea derivatives represent one class of the highly efficient and versatile PGRs.Specifically,forchlorfenuron(CPPU,N-(2-chloro-4-pyridinyl)-N0-phenylurea)exhibits similar growth-regulating efficacy to cytokinins and has a significant impact on the plant growth and the crop yield.As a result,there is growing interest in exploring the incorporation of various phenylurea moieties into agrochemicals to enhance their regulatory properties on crops.This review aims to provide a comprehensive overview on representative synthetic approaches for phenylurea derived PGRs.Additionally,we provide our perspective on the future development in this active researchfield.展开更多
Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ische...Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.展开更多
[Objectives]The paper was to establish an ultra high performance liquid chromatography-quadrupole/linear ion trap complex mass spectrometry for the determination of 10 kinds ofα2-receptor agonists in animal derived f...[Objectives]The paper was to establish an ultra high performance liquid chromatography-quadrupole/linear ion trap complex mass spectrometry for the determination of 10 kinds ofα2-receptor agonists in animal derived food.[Methods]The samples were extracted with sodium carbonate buffer solution and ethyl acetate,and analyzed by mass spectrometry after solid phase extraction and high performance liquid chromatography separation.[Results]Ten kinds ofα2-receptor agonists showed a good linear relationship in the range of 1-100μg/mL,with the average recovery of over 69%and the relative standard deviation less than 8.32%.The detection limit of 10 kinds of α_(2)-receptor agonists was up to 1μg/kg.[Conclusions]The method has good selectivity and strong anti-interference ability,and can meet the requirements of 10 kinds ofα2-receptor agonists residues in animal derived food.展开更多
In the past years, we established analytic expressions of various fractals and discussed Hölder derivatives of the expressions. Based on our earlier results, we will study the properties of harmonic functions on ...In the past years, we established analytic expressions of various fractals and discussed Hölder derivatives of the expressions. Based on our earlier results, we will study the properties of harmonic functions on a very important fractal, the Sierpinski gasket (SG). Our main result is that the harmonic function on SG satisfies a Hölder inequality of order α=ln35\ln2.展开更多
The research progress of brain-derived neurotrophic factor (BDNF) in the treatment of sequelae of stroke is an important topic. Stroke is among the diseases with the highest mortality and disability rates among the el...The research progress of brain-derived neurotrophic factor (BDNF) in the treatment of sequelae of stroke is an important topic. Stroke is among the diseases with the highest mortality and disability rates among the elderly in China. BDNF plays an important role in the development and functional maintenance of the nervous system. In recent years, the application value of BDNF in rehabilitation therapy has gradually received attention. This study has adopted a systematic literature review method, searched Chinese and English databases, screened relevant studies, and conducted data extraction and quality evaluation. This review systematically introduced the research progress of BDNF in the correlation with post-stroke sequelae, with special attention to its application in post-stroke depression, motor dysfunction, and cognitive dysfunction. The results showed that a decrease in BDNF levels is closely related to the exacerbation of depressive symptoms, limited recovery of motor dysfunction, and the occurrence of cognitive dysfunction. BDNF, as a key neurobiological factor, has shown significant potential in the rehabilitation treatment of stroke. By exploring the potential of BDNF as a therapeutic target to prevent and treat sequelae of ischemic stroke, the current research bottlenecks, and the development trends of future treatment strategies.展开更多
High-energy-density(HED)fuel(e.g.widely used JP-10 and RJ-4),featuring compact 3D polycyclic structure with high strain,is of critical importance for volume-limited military aircraft,since their high density and combu...High-energy-density(HED)fuel(e.g.widely used JP-10 and RJ-4),featuring compact 3D polycyclic structure with high strain,is of critical importance for volume-limited military aircraft,since their high density and combustion heat can provide more propulsion energy.To reduce the reliance on petroleum source,it is highly desirable to develop renewable alternatives for the production of strained polycyclic HED fuel,but which remains a big challenge because of the inaccessibility caused by the high strain,We herein demonstrate a three-step catalytic route towards highly strained C_(17)and C_(18)spirofuel with biomass feedstocks.The process includes catalytic aldol condensation of renewable cyclohexanone/cyclopentanone with benzaldehyde,catalytic spiro Diels-Alder(D-A)reaction of aldol adduct with isoprene,and catalytic hydrodeoxygenation.The key spiro D-A reaction is enabled by the catalysis of heterogeneous Lewis acidic ionic liquid.The chloroaluminate IL,formed by benign urea and AICl3,exhibits good catalytic performance and reusability for this step.An eventual hydrodeoxygenation(HDO)over Pd/C and H-Y produces strained tricyclic spirofuel with density>0.93 g/mL,combustion heat>41 MJ/L and freezing point<-40℃,which are better than the properties of tactical fuel RJ-4.Therefore,it is anticipated that the as-prepared renewable fuels have the potential to replace traditional petroleum-derived HED fuels.展开更多
A three-dimensional numerical model verified by previous experimental data is developed to simulate the fluidized bed gasification of refuse derived fuel （RDF）. The CaO dechlorination model obtained by the thermal g...A three-dimensional numerical model verified by previous experimental data is developed to simulate the fluidized bed gasification of refuse derived fuel （RDF）. The CaO dechlorination model obtained by the thermal gravity analysis （TGA） is coupled to investigate the process of CaO dechlorination. An Eulerian-Eulerian method is adopted to simulate the gas-solid flow and self-developed chemical reaction modules are used to simulate chemical reactions. Flow patterns, gasification results and dechlorination efficiency are obtained by numerical simulation. Meanwhile, simulations are performed to evaluate the effects of Ca/Cl molar ratio and temperature on dechlorination efficiency. The simulation results show that the presence of bubbles in the gasifier lowers the CaO dechlorination efficiency. Increasing the Ca/Cl molar ratio can enhance the dechlorination efficiency. However, with the temperature increasing, the dechlorination efficiency increases initially and then decreases. The optimal Ca/Cl molar ratio is in the range of 3. 0 to 3. 5 and the optimal temperature is 923K.展开更多
β-SiC ceramic powders were obtained by pyrolyzing polycarbosilane in vacuum at 800-1200 °C. The β-SiC ceramic powders were characterized by TGA/DSC, XRD and Raman spectroscopy. The dielectric properties of β-S...β-SiC ceramic powders were obtained by pyrolyzing polycarbosilane in vacuum at 800-1200 °C. The β-SiC ceramic powders were characterized by TGA/DSC, XRD and Raman spectroscopy. The dielectric properties of β-SiC ceramic powders were investigated by measuring their complex permittivity by rectangle wave guide method in the frequency range of 8.2-18 GHz. The results show that both real part ε′ and imaginary part ε″ of complex permittivity increase with increasing pyrolysis temperature. The mechanism was proposed that order carbon formed at high temperature resulted in electron relaxation polarization and conductance loss, which contributes to the increase in complex permittivity.展开更多
基金supported by the Natural Science Fund of Fujian Province,No.2020J011058(to JK)the Project of Fujian Provincial Hospital for High-level Hospital Construction,No.2020HSJJ12(to JK)+1 种基金the Fujian Provincial Finance Department Special Fund,No.(2021)848(to FC)the Fujian Provincial Major Scientific and Technological Special Projects on Health,No.2022ZD01008(to FC).
文摘Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological damage.Hypoxic preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are unknown.To this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB pathways.Subsequently,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by asphyxia.The results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these effects.To conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.
基金Supported by the Central South University Innovation-Driven Research Programme,No.2023CXQD075。
文摘Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational levels.tsRNAs are not only degradation fragments but also have biological functions,including those in immune inflammation,metabolic disorders,and cell death.tsRNA dysregulation is closely associated with multiple diseases,including various cancers and acute pancreatitis(AP).AP is a common gastrointestinal disease,and its incidence increases annually.AP development is associated with tsRNAs,which regulate cell injury and induce inflammation,especially pyroptosis and ferroptosis.Notably,serum tRF36 has the potential to serve as a non-invasive diagnostic biomarker and leads to pancreatic acinar cell ferroptosis causing inflammation to promote AP.We show the characteristics of tsRNAs and their diagnostic value and function in AP,and discuss the potential opportunities and challenges of using tsRNAs in clinical applications and research.
文摘Water contamination in areas of formal and informal polymetallic mining is a growing concern in the Peruvian highlands. At the same time, there are agricultural activities that use contaminated water from these rivers for irrigation. This could contaminate the agricultural products grown on these lands, such as potatoes. It is, therefore, necessary and a priority to determine the adsorption characteristics of these derivatives and to be able to use these natural adsorbents to decontaminate water contaminated with heavy metals.
文摘Autism spectrum disorder(ASD)is a complex neurodevelopmental disorder affecting over 2%of the global population,marked by social communication deficits and repetitive behaviors.Kabatas et al explored the efficacy and safety of Wharton’s jelly-derived mesenchymal stem cell(WJ-MSC)therapy in a 4-year-old child with ASD.Using the childhood autism rating scale and Denver II develop-mental screening test,significant improvements were seen after six WJ-MSC sessions,with no adverse events over 2 years.Despite promising results,the study’s single-case design limits generalizability.Larger,multi-center trials are needed to validate the findings and assess long-term effects of WJ-MSC therapy in ASD.
基金financially supported by the Natural Science Foundation of Guangdong province(2024A1515010228)the Research and demonstration application of intelligent sensing technology for lithium ion battery energy storage(SPICXJ-HTBBC-2022-01).
文摘Magnesium hydride(MgH_(2))is an important material for hydrogen(H_(2))storage and transportation owing to its high capacity and reversibility.However,its intrinsic properties have considerably limited its industrial application.In this study,the NiFe-800 catalyst as metal-organic framework(MOF)derivative was first utilized to promote the intrinsic properties of MgH_(2).Compared to pure MgH_(2),which releases1.24 wt%H_(2)in 60 min at 275℃,the MgH_(2)-10 NiFe-800 composite releases 5.85 wt%H_(2)in the same time.Even at a lower temperature of 250℃,the MgH_(2)-10 NiFe-800 composite releases 3.57 wt%H_(2),surpassing the performance of pure MgH_(2)at 275℃.Correspondingly,while pure MgH_(2)absorbs 2.08 wt%H_(2)in60 min at 125℃,the MgH_(2)-10 NiFe-800 composite absorbs 5.35 wt%H_(2)in just 1 min,Remarkably,the MgH_(2)-10 NiFe-800 composite absorbs 2.27 wt%H_(2)in 60 min at 50℃and 4.64 wt%H_(2)at 75℃.This indicates that MgH_(2)-10 NiFe-800 exhibits optimum performance with excellent kinetics at low temperatures.Furthermore,the capacity of the MgH_(2)-10 NiFe-800 composite remains largely stable after 10cycles.Moreover,the Mg_(2)Ni/Mg_(2)NiH_(4)acts as a"hydrogen pump",providing effective diffusion channels that enhance the kinetic process of the composite during cycling.Additionally,Fe0facilitates electron transfer and creates hydrogen diffusion channels and catalytic sites.Finally,carbon(C)effectively prevents particle agglomeration and maintains the cyclic stability of the composites.Consequently,the synergistic effects of Mg_(2)Ni/Mg_(2)NiH_(4),Fe^(0),and C considerably improve the kinetic properties and cycling stability of MgH_(2).This work offers an effective and valuable approach to improving the hydrogen storage efficiency in the commercial application of MgH_(2).
基金funded via National Natural Science Foundation of China(81925037,82321004,U22A20371,and 82273801)Guangdong Major Project of Basic and Applied Basic Research(2023B0303000026)+6 种基金Guangdong International Science and Technology Cooperation Base(2021A0505020015)Innovative and Research Teams Project of Guangdong Higher Education Institution(2021KCXTD001)Guangdong Basic and Applied Basic Research Foundation(2022A1515012071)Young Elite Scientists Sponsorship Program by China Association of Chinese Medicine(2021-QNRC2-B21)Guangdong-Hong Kong-Macao Universities Joint Laboratory for the Internationalization of Traditional Chinese Medicine(2023LSYS002)Guangzhou Key Laboratory of Traditional Chinese Medicine&Disease Susceptibility(2024A03J090)Science and Technology Program of Guangzhou(201707010414,202201020066,and 2024A04J9955)。
文摘Goji berry is a famous edible and medicinal substance around the world.In this research,15 phenylpropionyl phenylethylamine derivatives(1-15),in cluding one new compound(1),were separated and identified from g oji berry.All isolates were elucidated via ex tensive nuclear magnetic resonance spectral analyses and chemical techniques.Six known isolates were first obtained from Lycium genus.Six isolates were effectively split in to double chromatographic peaks accompanied by the basically identical areas,indicating they belong to racemates.The oxygen radical absorbance capacity(ORAC)experiment indicated that all isolates displayed a capacity of scavenging free radicals,and most of them exhibited higher ORAC than epigallocatechingallate.In ethanol/palmitic acid-established in vitro hepatocyte injury model,four phenylpropionyl phenylethylamine derivatives(1,2,9,and 15)significantly alleviated hepatocyte injury,among which compound 1 exerted the strongest protective activity.Notably,the hepatoprotective effect of compound 1 was further confirmed in ethanol-established liver damage mouse model,reflected by the reduction of lipid accumulation and the attenuation of pathological alteration.Combined with the in vitro results,the in vivo observations suggested that compound 1 suppressed cell apoptosis and the outburst of inflammation.Our findings provided first-hand evidence proving that goji berry-derived phenylpropionyl phenylethylamine derivatives hold a potential in treating alcoholic liver disease.
文摘Pulmonary fibrosis significantly contributes to the pathogenesis of acute respiratory distress syndrome(ARDS),markedly increasing patient mortality.Despite the established anti-fibrotic effects of mesenchymal stem cells(MSCs),numerous challenges hinder their clinical application.A recent study demon-strated that microvesicles(MVs)from MSCs(MSC-MVs)could attenuate ARDS-related pulmonary fibrosis and enhance lung function via hepatocyte growth factor mRNA transcription.This discovery presents a promising strategy for managing ARDS-associated pulmonary fibrosis.This article initially examines the safety and efficacy of MSCs from both basic science and clinical perspectives,subsequently exploring the potential and obstacles of employing MSC-MVs as a novel therapeutic approach.Additionally,it provides perspectives on future research into the application of MSC-MVs in ARDS-associated pulmonary fi-brosis.
基金Research Project of Jiangsu Provincial Health Commission,No.Z2022008and Research Project of Yangzhou Health Commission,No.2023-2-27.
文摘BACKGROUND Pulmonary fibrosis is one of the main reasons for the high mortality rate among acute respiratory distress syndrome(ARDS)patients.Mesenchymal stromal cell-derived microvesicles(MSC-MVs)have been shown to exert antifibrotic effects in lung diseases.AIM To investigate the effects and mechanisms of MSC-MVs on pulmonary fibrosis in ARDS mouse models.METHODS MSC-MVs with low hepatocyte growth factor(HGF)expression(siHGF-MSC-MVs)were obtained via lentivirus transfection and used to establish the ARDS pulmonary fibrosis mouse model.Following intubation,respiratory mechanics-related indicators were measured via an experimental small animal lung function tester.Homing of MSC-MVs in lung tissues was investigated by near-infrared live imaging.Immunohistochemical,western blotting,ELISA and other methods were used to detect expression of pulmonary fibrosis-related proteins and to compare effects on pulmonary fibrosis and fibrosis-related indicators.RESULTS The MSC-MVs gradually migrated and homed to damaged lung tissues in the ARDS model mice.Treatment with MSC-MVs significantly reduced lung injury and pulmonary fibrosis scores.However,low expression of HGF(siHGF-MSC-MVs)significantly inhibited the effects of MSC-MVs(P<0.05).Compared with the ARDS pulmonary fibrosis group,the MSC-MVs group exhibited suppressed expression of type I collagen antigen,type III collagen antigen,and the proteins transforming growth factor-βandα-smooth muscle actin,whereas the siHGF-MVs group exhibited significantly increased expression of these proteins.In addition,pulmonary compliance and the pressure of oxygen/oxygen inhalation ratio were significantly lower in the MSC-MVs group,and the effects of the MSC-MVs were significantly inhibited by low HGF expression(all P<0.05).CONCLUSION MSC-MVs improved lung ventilation functions and inhibited pulmonary fibrosis in ARDS mice partly via HGF mRNA transfer.
基金supported by the National Natural Science Foundation of China(52231007,12327804,T2321003,22088101)this work was supported in part by the National Key Research Program of China under Grant 2021YFA1200600,and Shanghai Sailing Program(22YF1447800).
文摘Broadband electromagnetic(EM)wave absorption materials play an important role in military stealth and health protection.Herein,metal–organic frameworks(MOFs)-derived magnetic-carbon CoNiM@C(M=Cu,Zn,Fe,Mn)microspheres are fabricated,which exhibit flower-like nano–microstructure with tunable EM response capacity.Based on the MOFs-derived CoNi@C microsphere,the adjacent third element is introduced into magnetic CoNi alloy to enhance EM wave absorption performance.In term of broadband absorption,the order of efficient absorption bandwidth(EAB)value is Mn>Fe=Zn>Cu in the CoNiM@C microspheres.Therefore,MOFs-derived flower-like CoNiMn@C microspheres hold outstanding broadband absorption and the EAB can reach up to 5.8 GHz(covering 12.2–18 GHz at 2.0 mm thickness).Besides,off-axis electron holography and computational simulations are applied to elucidate the inherent dielectric dissipation and magnetic loss.Rich heterointerfaces in CoNiMn@C promote the aggregation of the negative/positive charges at the contacting region,forming interfacial polarization.The graphitized carbon layer catalyzed by the magnetic CoNiMn core offered the electron mobility path,boosting the conductive loss.Equally importantly,magnetic coupling is observed in the CoNiMn@C to strengthen the magnetic responding behaviors.This study provides a new guide to build broadband EM absorption by regulating the ternary magnetic alloy.
基金Science and Technology Project of Hengshui,No.2019014061Z.
文摘BACKGROUND Stromal cell derived factor-1(SDF-1)plays a pivotal role in the recruitment of stem cells to injured livers.However,the changes of SDF-l in patients with hepatitis B virus(HBV)-related acute-on-chronic liver failure(ACLF)have yet to be elucidated.AIM To study the SDF-1 changes in patients with HBV-related ACLF.METHODS 30 patients with HBV-related ACLF,27 patients with chronic hepatitis B and 20 healthy individuals are involved in our study.The SDF-l mRNA expression in liver tissue was detected by quantitative real-time polymerase chain reaction.Immunohistochemical staining was performed to illustrate the expression of SDFl,CXC receptor 4(CXCR4)and Ki67.The serum SDF-l concentrations were also detected by enzyme-linked immunosorbent assays.RESULTS The expression of SDF-1 mRNA from ACLF patients was remarkably higher than that from other patients(both P<0.05).The expression of SDF-l,CXCR4 and Ki67 from ACLF were the highest among the three groups(all P<0.01).The serum SDF-l levels in ACLF patients were significantly lower than that in other patients(both P<0.01).Moreover,in ACLF patients,the serum SDF-1 Levels were positively correlated with serum total bilirubin and international normalized ratio.In addition,the serum SDF-l levels in survival were significantly lower compared with the non-survivals(P<0.05).The area under the curve for the serum SDF-1 level in predicting 28-d mortality was 0.722(P<0.05).CONCLUSION This study provides the SDF-1 changes in patients with HBV-related ACLF.The SDF-1 Level at admission may serve as a promising prognostic marker for predicting short-term prognosis.
基金support from the National Natural Science Foundation of China(22371058,21961006,32172459,22371057,22071036)the National Key Research and Development Program of China(2022YFD1700300)+3 种基金the Science and Technology Department of Guizhou Province(Qiankehejichu-ZK[2021]Key033)the Program of Introducing Talents of Discipline to Universities of China(111 Program,D20023)at Guizhou University,Frontiers Science Center for Asymmetric Synthesis and Medicinal MoleculesDepartment of Education,Guizhou Province[Qianjiaohe KY(2020)004]Guizhou University(China).
文摘Plant growth regulators(PGRs)are chemical substances that imitate the functions of phytohormones to enhance the crop yield and the harvest process.Phenylurea-derived plant growth regulators are known for their excellent efficacy in promoting fruit growth,particularly in kiwifruit,grapes,and melons.Phenylurea derivatives represent one class of the highly efficient and versatile PGRs.Specifically,forchlorfenuron(CPPU,N-(2-chloro-4-pyridinyl)-N0-phenylurea)exhibits similar growth-regulating efficacy to cytokinins and has a significant impact on the plant growth and the crop yield.As a result,there is growing interest in exploring the incorporation of various phenylurea moieties into agrochemicals to enhance their regulatory properties on crops.This review aims to provide a comprehensive overview on representative synthetic approaches for phenylurea derived PGRs.Additionally,we provide our perspective on the future development in this active researchfield.
基金supported by the National Natural Science Foundation of China,No.82001604Guizhou Provincial Higher Education Science and Technology Innovation Team,No.[2023]072+1 种基金Guizhou Province Distinguished Young Scientific and Technological Talent Program,No.YQK[2023]040Guizhou Provincial Basic Research Program(Natural Science),No.ZK[2021]-368(all to LXiong),and Zunyi City Innovative Talent Team Training Plan,No.[2022]-2.
文摘Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.
基金Supported by Scientific Research Project of Dalian Customs(2022DK09).
文摘[Objectives]The paper was to establish an ultra high performance liquid chromatography-quadrupole/linear ion trap complex mass spectrometry for the determination of 10 kinds ofα2-receptor agonists in animal derived food.[Methods]The samples were extracted with sodium carbonate buffer solution and ethyl acetate,and analyzed by mass spectrometry after solid phase extraction and high performance liquid chromatography separation.[Results]Ten kinds ofα2-receptor agonists showed a good linear relationship in the range of 1-100μg/mL,with the average recovery of over 69%and the relative standard deviation less than 8.32%.The detection limit of 10 kinds of α_(2)-receptor agonists was up to 1μg/kg.[Conclusions]The method has good selectivity and strong anti-interference ability,and can meet the requirements of 10 kinds ofα2-receptor agonists residues in animal derived food.
文摘In the past years, we established analytic expressions of various fractals and discussed Hölder derivatives of the expressions. Based on our earlier results, we will study the properties of harmonic functions on a very important fractal, the Sierpinski gasket (SG). Our main result is that the harmonic function on SG satisfies a Hölder inequality of order α=ln35\ln2.
文摘The research progress of brain-derived neurotrophic factor (BDNF) in the treatment of sequelae of stroke is an important topic. Stroke is among the diseases with the highest mortality and disability rates among the elderly in China. BDNF plays an important role in the development and functional maintenance of the nervous system. In recent years, the application value of BDNF in rehabilitation therapy has gradually received attention. This study has adopted a systematic literature review method, searched Chinese and English databases, screened relevant studies, and conducted data extraction and quality evaluation. This review systematically introduced the research progress of BDNF in the correlation with post-stroke sequelae, with special attention to its application in post-stroke depression, motor dysfunction, and cognitive dysfunction. The results showed that a decrease in BDNF levels is closely related to the exacerbation of depressive symptoms, limited recovery of motor dysfunction, and the occurrence of cognitive dysfunction. BDNF, as a key neurobiological factor, has shown significant potential in the rehabilitation treatment of stroke. By exploring the potential of BDNF as a therapeutic target to prevent and treat sequelae of ischemic stroke, the current research bottlenecks, and the development trends of future treatment strategies.
基金supported by the Fundamental Research Funds for the Central Universities(2023QN1009)Xuzhou Basic Research Project(KC23018)+1 种基金China University of Mining and Technology(CUMT)Open Sharing Fund for Large-scale Instruments and Equipment(DYGX-2024-34)Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘High-energy-density(HED)fuel(e.g.widely used JP-10 and RJ-4),featuring compact 3D polycyclic structure with high strain,is of critical importance for volume-limited military aircraft,since their high density and combustion heat can provide more propulsion energy.To reduce the reliance on petroleum source,it is highly desirable to develop renewable alternatives for the production of strained polycyclic HED fuel,but which remains a big challenge because of the inaccessibility caused by the high strain,We herein demonstrate a three-step catalytic route towards highly strained C_(17)and C_(18)spirofuel with biomass feedstocks.The process includes catalytic aldol condensation of renewable cyclohexanone/cyclopentanone with benzaldehyde,catalytic spiro Diels-Alder(D-A)reaction of aldol adduct with isoprene,and catalytic hydrodeoxygenation.The key spiro D-A reaction is enabled by the catalysis of heterogeneous Lewis acidic ionic liquid.The chloroaluminate IL,formed by benign urea and AICl3,exhibits good catalytic performance and reusability for this step.An eventual hydrodeoxygenation(HDO)over Pd/C and H-Y produces strained tricyclic spirofuel with density>0.93 g/mL,combustion heat>41 MJ/L and freezing point<-40℃,which are better than the properties of tactical fuel RJ-4.Therefore,it is anticipated that the as-prepared renewable fuels have the potential to replace traditional petroleum-derived HED fuels.
基金The National Natural Science Foundation of China(No.51476032)
文摘A three-dimensional numerical model verified by previous experimental data is developed to simulate the fluidized bed gasification of refuse derived fuel （RDF）. The CaO dechlorination model obtained by the thermal gravity analysis （TGA） is coupled to investigate the process of CaO dechlorination. An Eulerian-Eulerian method is adopted to simulate the gas-solid flow and self-developed chemical reaction modules are used to simulate chemical reactions. Flow patterns, gasification results and dechlorination efficiency are obtained by numerical simulation. Meanwhile, simulations are performed to evaluate the effects of Ca/Cl molar ratio and temperature on dechlorination efficiency. The simulation results show that the presence of bubbles in the gasifier lowers the CaO dechlorination efficiency. Increasing the Ca/Cl molar ratio can enhance the dechlorination efficiency. However, with the temperature increasing, the dechlorination efficiency increases initially and then decreases. The optimal Ca/Cl molar ratio is in the range of 3. 0 to 3. 5 and the optimal temperature is 923K.
基金Project (50572090) supported by the National Natural Science Foundation of ChinaProject (KP200901) supported by the Fund of the State Key Laboratory of Solidification Processing, China
文摘β-SiC ceramic powders were obtained by pyrolyzing polycarbosilane in vacuum at 800-1200 °C. The β-SiC ceramic powders were characterized by TGA/DSC, XRD and Raman spectroscopy. The dielectric properties of β-SiC ceramic powders were investigated by measuring their complex permittivity by rectangle wave guide method in the frequency range of 8.2-18 GHz. The results show that both real part ε′ and imaginary part ε″ of complex permittivity increase with increasing pyrolysis temperature. The mechanism was proposed that order carbon formed at high temperature resulted in electron relaxation polarization and conductance loss, which contributes to the increase in complex permittivity.
