Acinetobocter baumannfi （A. Baumannii） is an emerging opportunistic pathogen responsible for hospital-acquired infections, and which now constitutes a sufficiently serious threat to public health to necessitate the ...Acinetobocter baumannfi （A. Baumannii） is an emerging opportunistic pathogen responsible for hospital-acquired infections, and which now constitutes a sufficiently serious threat to public health to necessitate the development of an effective vaccine. In this study, a recombinant fused protein named OmpK/Omp22 and two individual proteins OmpK and Omp22 were obtained using recombinant expression and Ni-affinity purification. Groups of BALB/c mice were immunized with these proteins and challenged with a clinically isolated strain of A. boumonnii. The bacterial load in the blood, pathological changes in the lung tissue and survival rates after challenge were evaluated. Mice immunized with OmpK/Omp22 fused protein provided significantly greater protection against A. boumonnfi challenge than those immunized with either of the two proteins individually. The results provide novel clues for future design of vaccines against A. boumonnii.展开更多
为了探讨牛布鲁氏菌544A株感染巨噬细胞的c DNA文库中筛选与Omp22相互作用的蛋白,对布鲁氏菌的致病机理进行研究。将布鲁氏菌细胞膜可分为3组：第1组为94 k Da的外膜蛋白,第2组为41-43 k Da外膜蛋白,第3组为22-31 k Da的外膜蛋白。第1...为了探讨牛布鲁氏菌544A株感染巨噬细胞的c DNA文库中筛选与Omp22相互作用的蛋白,对布鲁氏菌的致病机理进行研究。将布鲁氏菌细胞膜可分为3组：第1组为94 k Da的外膜蛋白,第2组为41-43 k Da外膜蛋白,第3组为22-31 k Da的外膜蛋白。第1组外膜蛋白只是外膜蛋白的一小部分;第2组外膜蛋白是布鲁氏菌细胞外膜孔道蛋白;第3组外膜蛋白是由两种相关的22 k Da蛋白和31 k Da蛋白组成,31 k Da蛋白已经被证实了是一类细胞外膜孔道蛋白,而22 k Da蛋白（Omp22）的功能还不是很清楚。酵母双杂交技术是一种常用的而且有效地研究蛋白-蛋白相互作用的方法,已经广泛的应用到蛋白研究领域。本实验采用Cyto Tra P酵母双杂交系统,以Omp22为诱饵,从牛布鲁氏菌544A株感染巨噬细胞的c DNA文库中筛选与Omp22相互作用的蛋白,对布鲁氏菌的致病机理进行阐述,探索Omp22在布鲁氏菌致病中的作用。展开更多
基金supported by a project from Educational Commission of Sichuan Province of China[No 17ZA0166]
文摘Acinetobocter baumannfi （A. Baumannii） is an emerging opportunistic pathogen responsible for hospital-acquired infections, and which now constitutes a sufficiently serious threat to public health to necessitate the development of an effective vaccine. In this study, a recombinant fused protein named OmpK/Omp22 and two individual proteins OmpK and Omp22 were obtained using recombinant expression and Ni-affinity purification. Groups of BALB/c mice were immunized with these proteins and challenged with a clinically isolated strain of A. boumonnii. The bacterial load in the blood, pathological changes in the lung tissue and survival rates after challenge were evaluated. Mice immunized with OmpK/Omp22 fused protein provided significantly greater protection against A. boumonnfi challenge than those immunized with either of the two proteins individually. The results provide novel clues for future design of vaccines against A. boumonnii.
文摘为了探讨牛布鲁氏菌544A株感染巨噬细胞的c DNA文库中筛选与Omp22相互作用的蛋白,对布鲁氏菌的致病机理进行研究。将布鲁氏菌细胞膜可分为3组：第1组为94 k Da的外膜蛋白,第2组为41-43 k Da外膜蛋白,第3组为22-31 k Da的外膜蛋白。第1组外膜蛋白只是外膜蛋白的一小部分;第2组外膜蛋白是布鲁氏菌细胞外膜孔道蛋白;第3组外膜蛋白是由两种相关的22 k Da蛋白和31 k Da蛋白组成,31 k Da蛋白已经被证实了是一类细胞外膜孔道蛋白,而22 k Da蛋白（Omp22）的功能还不是很清楚。酵母双杂交技术是一种常用的而且有效地研究蛋白-蛋白相互作用的方法,已经广泛的应用到蛋白研究领域。本实验采用Cyto Tra P酵母双杂交系统,以Omp22为诱饵,从牛布鲁氏菌544A株感染巨噬细胞的c DNA文库中筛选与Omp22相互作用的蛋白,对布鲁氏菌的致病机理进行阐述,探索Omp22在布鲁氏菌致病中的作用。
