Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain stu...Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods.However,high-resolution imaging in optical microscopy fundamentally requires a tight optical focus,and thus a limited depth of field(DOF).Consequently,large-scale,non-stitched,high-resolution images of curved surfaces,like brains,are difficult to acquire without z-axis scanning.To overcome this limitation,we developed a needle-shaped beam optical coherence tomography angiography(NB-OCTA)system,and for the first time,achieved a volumetric resolution of less than 8μm in a non-stitched volume space of 6.4 mm×4 mm×620μm in vivo.This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam(GB-OCTA).We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo,and quantitatively analyzed the vessel area density(VAD)and the diameters of representative vessels in different regions over 10 days,revealing different spatiotemporal dynamics in the acute,sub-acute and chronic phase of post-ischemic revascularization.Benefiting from our NB-OCTA,we revealed that the recovery process is not only the result of spontaneous reperfusion,but also the formation of new vessels.This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.展开更多
Objective: To observe the therapeutic effect and safety of using the hooked needle-shaped knife to treat the stenosing tenovaginitis of flexor digitorum. Methods: Sixty outpatients were divided into a treatment group ...Objective: To observe the therapeutic effect and safety of using the hooked needle-shaped knife to treat the stenosing tenovaginitis of flexor digitorum. Methods: Sixty outpatients were divided into a treatment group of 30 cases treated by using the hooked needle shaped knife, and a control group of 30 cases treated by block therapy. 6 months later, the alleviation of pain in the affected finger during movement, under pressure, traction and finger-bending anti-resistance was compared before and after treatment. Results: The alleviation of pain during movement, under pressure, traction and finger-bending anti-resistance was much better in the treatment group than that of the control group (P<0.01), with a effective rate of 93.3% in the treatment group and 80.0% in the control group (P<0.01). Conclusion: The micro-wound technique using the hooked needle-shaped knife has definite effect and safety for stenosing tenovaginitis of flexor digitorum.展开更多
Objectives: To evaluate endoscopic duodenal sphinc- terotomy and improve its success rate. Methods: Needle-shaped knife was used for endoscop- ic sphincterotomy (EST) in 476 patients with biliary or pancreatic disease...Objectives: To evaluate endoscopic duodenal sphinc- terotomy and improve its success rate. Methods: Needle-shaped knife was used for endoscop- ic sphincterotomy (EST) in 476 patients with biliary or pancreatic diseases from March 1995 to October 2000. Results: Direct incision was made in 243 patients, papillary fenestration in 89, and opposite incision in 144. The papilla located beside and in the diverticu- lum in 56 and 12 patients, respectively. EST emer- gency was made in 147 patients. ERCP after EST be- cause of the difficulty in intubation was made suc- cessfully in 62 patients. Mild complications occurred in 14 patients (2.94%), all of whom were cured af- ter symptomatic treatment. Conclusions: EST with needle-shaped knife has such advantages as safety in operation and convenience in incision. It is suitable for the papilla with different shape, with a higher success rate.展开更多
Ce3+ doped Bi2O3 hollow needle-shape with enhanced visible-light photocatalytic activity was successfully prepared via the method of chemical precipitation using Bi(NO3)·5H2O and Ce(NO3)3·6H2O as the source ...Ce3+ doped Bi2O3 hollow needle-shape with enhanced visible-light photocatalytic activity was successfully prepared via the method of chemical precipitation using Bi(NO3)·5H2O and Ce(NO3)3·6H2O as the source of bismuth and cerium, HNO3 as solvent and NaOH as precipitants, respectively and after calcination at 500 ℃ for 2 h. The morphology and elemental composition,crystal form,purity and specific surface area of the hollow needle Bi2O3 were characterized by SEM-EDS, XRD, BET and FT-IR. The photocatalytic properties of the as-obtained samples were measured by UV-vis diffuse reflection spectroscopy and photochemical reactor. As a result, the obtained Bi2O3 hollow needle-shape doped with 5 wt% Ce shows good morphology, α-phase, stronger absorbent for visible light and good photocatalytic property. Under the simulated visible light of 300 W, the photodegradation rate of tetracycline over HNBCe can reach to 89.1%,which is higher than that of commercial Bi2O3 nanoparticles and Bi2O3 hollow needle-shape.展开更多
基金supported by the National Key R&D Program of China(No.2022YFB4702902)National Natural Science Foundation of China(Nos.61831014,62275023,and 32021002)+2 种基金Beijing Municipal Natural Science Foundation(No.4232077)Overseas Expertise Introduction Project for Discipline Innovation(No.B18005)STI2030-Major Projects(No.2022ZD0212000).
文摘Optical imaging techniques provide low-cost,non-radiative images with high spatiotemporal resolution,making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods.However,high-resolution imaging in optical microscopy fundamentally requires a tight optical focus,and thus a limited depth of field(DOF).Consequently,large-scale,non-stitched,high-resolution images of curved surfaces,like brains,are difficult to acquire without z-axis scanning.To overcome this limitation,we developed a needle-shaped beam optical coherence tomography angiography(NB-OCTA)system,and for the first time,achieved a volumetric resolution of less than 8μm in a non-stitched volume space of 6.4 mm×4 mm×620μm in vivo.This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam(GB-OCTA).We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo,and quantitatively analyzed the vessel area density(VAD)and the diameters of representative vessels in different regions over 10 days,revealing different spatiotemporal dynamics in the acute,sub-acute and chronic phase of post-ischemic revascularization.Benefiting from our NB-OCTA,we revealed that the recovery process is not only the result of spontaneous reperfusion,but also the formation of new vessels.This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.
文摘Objective: To observe the therapeutic effect and safety of using the hooked needle-shaped knife to treat the stenosing tenovaginitis of flexor digitorum. Methods: Sixty outpatients were divided into a treatment group of 30 cases treated by using the hooked needle shaped knife, and a control group of 30 cases treated by block therapy. 6 months later, the alleviation of pain in the affected finger during movement, under pressure, traction and finger-bending anti-resistance was compared before and after treatment. Results: The alleviation of pain during movement, under pressure, traction and finger-bending anti-resistance was much better in the treatment group than that of the control group (P<0.01), with a effective rate of 93.3% in the treatment group and 80.0% in the control group (P<0.01). Conclusion: The micro-wound technique using the hooked needle-shaped knife has definite effect and safety for stenosing tenovaginitis of flexor digitorum.
文摘Objectives: To evaluate endoscopic duodenal sphinc- terotomy and improve its success rate. Methods: Needle-shaped knife was used for endoscop- ic sphincterotomy (EST) in 476 patients with biliary or pancreatic diseases from March 1995 to October 2000. Results: Direct incision was made in 243 patients, papillary fenestration in 89, and opposite incision in 144. The papilla located beside and in the diverticu- lum in 56 and 12 patients, respectively. EST emer- gency was made in 147 patients. ERCP after EST be- cause of the difficulty in intubation was made suc- cessfully in 62 patients. Mild complications occurred in 14 patients (2.94%), all of whom were cured af- ter symptomatic treatment. Conclusions: EST with needle-shaped knife has such advantages as safety in operation and convenience in incision. It is suitable for the papilla with different shape, with a higher success rate.
基金Project supported by the Capacity Building Program of Shanghai Local Universities(12160503600)
文摘Ce3+ doped Bi2O3 hollow needle-shape with enhanced visible-light photocatalytic activity was successfully prepared via the method of chemical precipitation using Bi(NO3)·5H2O and Ce(NO3)3·6H2O as the source of bismuth and cerium, HNO3 as solvent and NaOH as precipitants, respectively and after calcination at 500 ℃ for 2 h. The morphology and elemental composition,crystal form,purity and specific surface area of the hollow needle Bi2O3 were characterized by SEM-EDS, XRD, BET and FT-IR. The photocatalytic properties of the as-obtained samples were measured by UV-vis diffuse reflection spectroscopy and photochemical reactor. As a result, the obtained Bi2O3 hollow needle-shape doped with 5 wt% Ce shows good morphology, α-phase, stronger absorbent for visible light and good photocatalytic property. Under the simulated visible light of 300 W, the photodegradation rate of tetracycline over HNBCe can reach to 89.1%,which is higher than that of commercial Bi2O3 nanoparticles and Bi2O3 hollow needle-shape.
