The use of allelopathic plants is an alternative technique for controlling weeds in sustainable crop production. This experiment evaluated the allelopathic potential of Murraya paniculata （L.） against four weeds viz...The use of allelopathic plants is an alternative technique for controlling weeds in sustainable crop production. This experiment evaluated the allelopathic potential of Murraya paniculata （L.） against four weeds viz., Bidens pilosa L., Amarathus spinosus L., Echinochloa crusgalli （L.） Beauv. and Chloris barbata Sw.. In laboratory test, aqueous extracts from M. paniculata leaves at concentrations of 12.5, 25, 50 and 100 g/L were tested on germination and seedling growth of the weeds. The extracts showed inhibitory effect, varying with weed species and extract concentration. Increase in concentrations increased the degree of inhibition. Concentration at 50 g/L and 100 g/L, completely inhibited seed germination of C. barbara and A. spinosus. Pot experiments were done in a glasshouse, with dry leaf powder as mulch on the soil surface at levels of 2, 4 and 8 t/ha. Dry leaf powder reduced the emergence and growth of all tested weeds, varying with dose and species. Chloris barbara is the most sensitive to M paniculata leaf mulch while E. crus-galli is the most resistant. These results suggest that M. paniculata had the potential lbr weed control and might be the source of a natural herbicide.展开更多
Four hitherto unknown prenylated coumarins,namely 600-O-b-D-apiofuranosylapterin(1),40-O-isobutyroylpeguangxienin(2),6-(3-methyl-2-oxobutyroyl)-7-methoxycoumarin(3),and 6-hydroxycoumurrayin(4),were isolated from the ...Four hitherto unknown prenylated coumarins,namely 600-O-b-D-apiofuranosylapterin(1),40-O-isobutyroylpeguangxienin(2),6-(3-methyl-2-oxobutyroyl)-7-methoxycoumarin(3),and 6-hydroxycoumurrayin(4),were isolated from the ethanol extract of Heracleum stenopterum,Peucedanum praeruptorum,Clausena lansium,and Murraya paniculata,respectively.Their chemical structures were established on the basis of extensive spectroscopic analysis.Compound 2 exhibited in vitro cytotoxic activity against five human cancer cell lines(HL-60,A-549,SMMC-7721,MCF-7,and SW-480)with IC50 values ranging from 15.9 to 23.2μM.展开更多
满天星是当今世界最流行的鲜切花配花之一,茎腐病是满天星的一种常见病害,严重时导致植株枯萎死亡,影响鲜花的观赏性和利用价值,造成一定的经济损失。2021年3月,笔者在云南省昆明市海口镇发现满天星茎腐病,该病主要为害茎基部,表现为茎...满天星是当今世界最流行的鲜切花配花之一,茎腐病是满天星的一种常见病害,严重时导致植株枯萎死亡,影响鲜花的观赏性和利用价值,造成一定的经济损失。2021年3月,笔者在云南省昆明市海口镇发现满天星茎腐病,该病主要为害茎基部,表现为茎基部腐烂和萎蔫。为明确其病原,本研究对满天星茎腐病病原菌进行分离与鉴定。柯赫氏法则证明该分离物可造成满天星组培苗和盆栽苗表现出与田间病株相似的症状,经接种后获得的分离物与田间自然发病的分离物一致。通过形态学鉴定,分离物能产生大量致密的气生菌丝,颜色从白色到淡橙色或黄色不等,菌落背面有暗红色色素沉积。显微镜观察发现其分离物的分生孢子呈镰刀形,腹面较平直,背侧呈拱形,具有3~6个隔膜,分生孢子大小为(21.1~57.9)μm×(2.7~5.1)μm(n=100),未见小型分生孢子,形态学上与镰刀菌属病原真菌相似。通过对其ITS基因序列与形态学鉴定,将分离物初步确定为禾谷镰孢复合种(Fusarium graminearum species complex,FGSC),为进一步确定亚种,使用翻译延伸因子(TEF-1α)序列进行测序,发现该分离物与南方镰孢菌(F.meridionale)的同源性达100%。因此结合形态学和分子生物学特征,满天星茎腐病的病原确定为南方镰孢菌。目前国内尚未发现由南方镰孢菌侵染为害满天星茎部的相关研究报道,本研究结果将为防治满天星病害提供重要理论依据。展开更多
OBJECTIVE： Different parts of Murraya paniculata have been used traditionally for treating several ailments including mental disorders. The present study was designed to evaluate the antianxiety and antidepressant po...OBJECTIVE： Different parts of Murraya paniculata have been used traditionally for treating several ailments including mental disorders. The present study was designed to evaluate the antianxiety and antidepressant potential of M. paniculata leaves using elevated plus maze model and forced swim test, respectively. METHODS： Extracts of M. panicu/ata made with petroleum ether （60-80 ℃）, chloroform, ethanol and water were evaluated for antianxiety and antidepressant activity. The anxiolytic chloroform extract was subjected to column chromatography, yielding five fractions （F1-F5）. Fraction F5 （100 mg/kg）, which showed notable anxiolytic activity, was further chromatographed to get four subfractions （F5.1-F5.4）. Simultaneously, the ethano extract was partitioned with ethyl acetate to obtain ethyl acetate soluble fraction （EASF） and ethyl acetate insoluble fraction. Phytochemical screening of bioactive extracts/fractions and detection of mahanimbine in M paniculata leaf extract by thin-layer chromatography was also carried out. RESULTS： Fraction F5.3 （25 mg/kg） and EASF （20 mg/kg） showed significant anxiolytic and antidepressant activity, respectively. Thin-layer chromatography and phytochemical screening demonstrated the absence of mahanimbine in M. paniculata leaves. Coumarins were observed to be responsible for the anxiolytic activity. CONCLUSION： The results validate the traditional use of M. paniculata leaves in the treatment of menta disorders.展开更多
There are two source plants for the traditional Chinese medicine Murrayae Folium et Cacumen(MFC)in Chinese Pharmacopoeia,i.e.Murraya exotica L.and M.paniculata(L.)Jack.Herein,a chemical comparison of M.exotica and M.p...There are two source plants for the traditional Chinese medicine Murrayae Folium et Cacumen(MFC)in Chinese Pharmacopoeia,i.e.Murraya exotica L.and M.paniculata(L.)Jack.Herein,a chemical comparison of M.exotica and M.paniculata by high performance liquid chromatography(HPLC)fingerprint analysis coupled with chemometrics and network pharmacology was performed.The main peaks in the fingerprints were identified by liquid chromatography coupled with ion trap/time-of-flight mass spectrometry(LC-IT-TOF-MS)and authenticated by references.The chemometrics results showed that the HPLC fingerprints of these two species were clearly divided into two categories using hierarchical cluster analysis(HCA)and principal component analysis(PCA),and a total of 13 significantly differentiated markers were screened out by orthogonal partial least squares-discriminant analysis(OPLS-DA).However,the following network pharmacology analysis showed that these discriminated markers were found to act via many common targets and metabolic pathways,indicating the possibly similar pharmacological effects and mechanisms for M.exotica and M.paniculata.The above results provide valuable evidence for the equivalent use of these two plants in clinical settings.Moreover,the chromatographic fingerprint analysis coupled with chemometrics and network pharmacology supplies an efficient approach for the comparative analysis of multi-source TCMs like MFC.展开更多
文摘The use of allelopathic plants is an alternative technique for controlling weeds in sustainable crop production. This experiment evaluated the allelopathic potential of Murraya paniculata （L.） against four weeds viz., Bidens pilosa L., Amarathus spinosus L., Echinochloa crusgalli （L.） Beauv. and Chloris barbata Sw.. In laboratory test, aqueous extracts from M. paniculata leaves at concentrations of 12.5, 25, 50 and 100 g/L were tested on germination and seedling growth of the weeds. The extracts showed inhibitory effect, varying with weed species and extract concentration. Increase in concentrations increased the degree of inhibition. Concentration at 50 g/L and 100 g/L, completely inhibited seed germination of C. barbara and A. spinosus. Pot experiments were done in a glasshouse, with dry leaf powder as mulch on the soil surface at levels of 2, 4 and 8 t/ha. Dry leaf powder reduced the emergence and growth of all tested weeds, varying with dose and species. Chloris barbara is the most sensitive to M paniculata leaf mulch while E. crus-galli is the most resistant. These results suggest that M. paniculata had the potential lbr weed control and might be the source of a natural herbicide.
文摘Four hitherto unknown prenylated coumarins,namely 600-O-b-D-apiofuranosylapterin(1),40-O-isobutyroylpeguangxienin(2),6-(3-methyl-2-oxobutyroyl)-7-methoxycoumarin(3),and 6-hydroxycoumurrayin(4),were isolated from the ethanol extract of Heracleum stenopterum,Peucedanum praeruptorum,Clausena lansium,and Murraya paniculata,respectively.Their chemical structures were established on the basis of extensive spectroscopic analysis.Compound 2 exhibited in vitro cytotoxic activity against five human cancer cell lines(HL-60,A-549,SMMC-7721,MCF-7,and SW-480)with IC50 values ranging from 15.9 to 23.2μM.
文摘满天星是当今世界最流行的鲜切花配花之一,茎腐病是满天星的一种常见病害,严重时导致植株枯萎死亡,影响鲜花的观赏性和利用价值,造成一定的经济损失。2021年3月,笔者在云南省昆明市海口镇发现满天星茎腐病,该病主要为害茎基部,表现为茎基部腐烂和萎蔫。为明确其病原,本研究对满天星茎腐病病原菌进行分离与鉴定。柯赫氏法则证明该分离物可造成满天星组培苗和盆栽苗表现出与田间病株相似的症状,经接种后获得的分离物与田间自然发病的分离物一致。通过形态学鉴定,分离物能产生大量致密的气生菌丝,颜色从白色到淡橙色或黄色不等,菌落背面有暗红色色素沉积。显微镜观察发现其分离物的分生孢子呈镰刀形,腹面较平直,背侧呈拱形,具有3~6个隔膜,分生孢子大小为(21.1~57.9)μm×(2.7~5.1)μm(n=100),未见小型分生孢子,形态学上与镰刀菌属病原真菌相似。通过对其ITS基因序列与形态学鉴定,将分离物初步确定为禾谷镰孢复合种(Fusarium graminearum species complex,FGSC),为进一步确定亚种,使用翻译延伸因子(TEF-1α)序列进行测序,发现该分离物与南方镰孢菌(F.meridionale)的同源性达100%。因此结合形态学和分子生物学特征,满天星茎腐病的病原确定为南方镰孢菌。目前国内尚未发现由南方镰孢菌侵染为害满天星茎部的相关研究报道,本研究结果将为防治满天星病害提供重要理论依据。
基金funded by the University Grant Commission,New Delhi,India-110002(Grant number F.4-1/2006(BSR)/5-94/2007)
文摘OBJECTIVE： Different parts of Murraya paniculata have been used traditionally for treating several ailments including mental disorders. The present study was designed to evaluate the antianxiety and antidepressant potential of M. paniculata leaves using elevated plus maze model and forced swim test, respectively. METHODS： Extracts of M. panicu/ata made with petroleum ether （60-80 ℃）, chloroform, ethanol and water were evaluated for antianxiety and antidepressant activity. The anxiolytic chloroform extract was subjected to column chromatography, yielding five fractions （F1-F5）. Fraction F5 （100 mg/kg）, which showed notable anxiolytic activity, was further chromatographed to get four subfractions （F5.1-F5.4）. Simultaneously, the ethano extract was partitioned with ethyl acetate to obtain ethyl acetate soluble fraction （EASF） and ethyl acetate insoluble fraction. Phytochemical screening of bioactive extracts/fractions and detection of mahanimbine in M paniculata leaf extract by thin-layer chromatography was also carried out. RESULTS： Fraction F5.3 （25 mg/kg） and EASF （20 mg/kg） showed significant anxiolytic and antidepressant activity, respectively. Thin-layer chromatography and phytochemical screening demonstrated the absence of mahanimbine in M. paniculata leaves. Coumarins were observed to be responsible for the anxiolytic activity. CONCLUSION： The results validate the traditional use of M. paniculata leaves in the treatment of menta disorders.
基金supported by the National Natural Sciences Foundation of China(Nos.81773864,81973199,and81473106)the National Key Research and Development Project(No.2019YFC1711000)。
文摘There are two source plants for the traditional Chinese medicine Murrayae Folium et Cacumen(MFC)in Chinese Pharmacopoeia,i.e.Murraya exotica L.and M.paniculata(L.)Jack.Herein,a chemical comparison of M.exotica and M.paniculata by high performance liquid chromatography(HPLC)fingerprint analysis coupled with chemometrics and network pharmacology was performed.The main peaks in the fingerprints were identified by liquid chromatography coupled with ion trap/time-of-flight mass spectrometry(LC-IT-TOF-MS)and authenticated by references.The chemometrics results showed that the HPLC fingerprints of these two species were clearly divided into two categories using hierarchical cluster analysis(HCA)and principal component analysis(PCA),and a total of 13 significantly differentiated markers were screened out by orthogonal partial least squares-discriminant analysis(OPLS-DA).However,the following network pharmacology analysis showed that these discriminated markers were found to act via many common targets and metabolic pathways,indicating the possibly similar pharmacological effects and mechanisms for M.exotica and M.paniculata.The above results provide valuable evidence for the equivalent use of these two plants in clinical settings.Moreover,the chromatographic fingerprint analysis coupled with chemometrics and network pharmacology supplies an efficient approach for the comparative analysis of multi-source TCMs like MFC.
基金supported by the Research Platform for Quality Standard of TCMs and Information System Building(No.2009ZX09308-004)National S&T Major Project-Created Major New Drugs Projects(No.2010ZX09502-002)~~
