[Objectives]To explore the mechanism of Mongolian medicine Valeriana officinalis L.on liver cancer by network pharmacology.[Methods]The HERB database of V.officinalis L.was searched,and the Uniprot database was used t...[Objectives]To explore the mechanism of Mongolian medicine Valeriana officinalis L.on liver cancer by network pharmacology.[Methods]The HERB database of V.officinalis L.was searched,and the Uniprot database was used to normalize and standardize the targets.Liver cancer targets were searched through GeneCards,DISGENET,and other databases.Venny website was used to obtain the intersection target of valerian active ingredients and liver cancer disease.The protein-protein interaction(PPI)network of the intersection targets was analyzed by STRING database,and the PPI network was constructed by Cytoscape software.The David database was used for GO functional annotation and KEGG pathway enrichment analysis to obtain the relevant pathways in the treatment of liver cancer with Mongolian medicine V.officinalis.The corresponding chemical components,targets and pathways of liver cancer were imported into Cytoscape software to construct the network topology of"chemical component-disease-target-pathway".According to the analysis results,the potential of the active components in V.officinalis as a therapeutic drug for liver cancer was evaluated,and the correlation between the results of network pharmacology analysis and clinical treatment of liver cancer was discussed,which provided a reference for clinical application.[Results]A total of 13 kinds of chemical components and 108 drug disease intersection target genes were screened,and the core genes acting on diseases were caffeic acid,perillyl acetate,(+)-alpha-Terpineol,eucalyptol,etc.;GO functional enrichment analysis involved 389 items of biological processes,62 items of cellular components and 120 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out chemical carcinogenesis-receptor activation,cancer pathways,prolactin signaling pathways,proteoglycans in cancer,EGFR tyrosine kinase inhibitor resistance and other signaling pathways.[Conclusions]The mechanism of Mongolian medicine V.officinalis on liver cancer was studied by network pharmacology.It was found that it can inhibit the proliferation of liver cancer cells from multiple targets and pathways.This is expected to provide a theoretical basis for further basic experimental research.展开更多
Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative act...Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative action of a delay in yolk sac absorption by Bajitian was investigated by RT-PCR analysis of lipid metabolism-related microsomal triglyceride transfer protein(MTP),apolipoprotein CII(ApoC2)and lipogenesis-related liver x receptor(LXR)genes.The effect of Bajitian eliciting an inflammatory response was studied by exposing 72 hpf myeloperoxidase(MPO):GFP transgenic zebrafish embryos to Bajitian extract for 4 h.Assessment was done by TUNEL,caspase-3/7,and RT-PCR analysis of the apoptosis related pathway B-cell lymphoma 2 associated X protein(Bax),Nuclear factor kappa-lightchain-enhancer of activated B cells(NF-k B)genes,neutrophil development-related stem cell leukaemia(SCL)and transcription factor PU.1 genes,to reveal the causative action of Bajitian reducing neutrophils.Results:RT-PCR analysis found that Bajitian extract had no effect on the expression of MTP or ApoC2 genes,but upregulated LXR gene,which might explain the delay in yolk sac absorption.Analysis of the inflammatory response showed that compared with negative controls,Bajitian extract significantly(P<.05)reduced the number of neutrophils in MPO:GFP embryos.TUNEL,caspase-3/7,and RT-PCR analysis of Bax and NF-k B genes found that Bajitian extract did not trigger the cell apoptosis.Further RT-PCR analysis found that Bajitian extract did not affect SCL expression,but did lead to down-regulation of PU.1.The inhibition of neutrophil development/differentiation may explain the decline in the total number of neutrophils following Bajitian treatment,which could be attributed to the anti-inflammatory effects found clinically for this drug.Conclusions:This study demonstrated that Bajitian caused a delay in yolk sac absorption and a decrease neutrophil in zebrafish embryos,which may be related to the inhibition of neutrophil development.展开更多
Herbal extraction residues(HERs)cause serious environmental pollution and resource waste.In this study,a novel green route was designed for the comprehensive reutilization of all components in HERs,taking Magnolia off...Herbal extraction residues(HERs)cause serious environmental pollution and resource waste.In this study,a novel green route was designed for the comprehensive reutilization of all components in HERs,taking Magnolia officinalis residues(MOR)as an example.The reluctant structure of MOR was first destroyed by alkali pretreatment to release the functional ingredients(magnolol and honokiol)originally remaining in MOR and to make MOR more accessible for hydrolysis.A metal–organic frame material MIL-101(Cr)with a maximum absorption capacity of 255.64 mg g^(-1)was synthesized to absorb the released honokiol and magnolol from the pretreated MOR solutions,and 40 g L^(-1)reducing sugars were obtained with 81.8%enzymatic hydrolysis rate at 10%MOR solid loading.Finally,382 mg L-1β-amyrin was produced from MOR hydrolysates by an engineered yeast strain.In total,1 kg honokiol,8 kg magnolol,and 7.64 kg β-amyrin could produce from 1 ton MOR by this cleaner process with a total economic output of 170,700 RMB.展开更多
The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, ...The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, could be one way of building resilience in vulnerable farming households. The aim of this study was to determine the secondary metabolite and mineral composition of noni juice obtained by fermenting the fruit of Morinda citrifolia L. Fruits were collected in August 2022 from the local field in Thiès region, West of Senegal. Extraction yields were determined and the secondary metabolites were determined using conventional analytical methods. Calcium, magnesium, iron, sodium and potassium were determined by atomic absorption spectrophotometer coupled with a CCD detector. The results show that an average fruit mass (503.2 ± 110.96 g) consists of 171.44 ± 50.01 g pulp and 34.06 ± 10.35 g seeds. The traditional extraction yield of noni juice is 16.46% after three weeks of fermentation. The contents of total polyphenols, flavonoids and tannins obtained in noni are 608.97 ± 4.53 mg EAG/100mL, 7.78 ± 0.01 mg EQ/100mL and 0.191 ± 0.01 mg EC/100mL respectively. The ethanol content of noni varies from 3.57 to 5.23 mL/100mL during extraction. Noni has a high calcium content with a concentration of 383.79 ± 33.23 mg/L. This is followed by a good concentration of magnesium, potassium and sodium, at 278.47 ± 26.30, 187.43 ± 10.7 and 155.95 ± 28.66 mg/L respectively. Noni also has an iron content of 202.15 ± 0.05 mg/L.展开更多
Cornus officinalis,a medicinal and edible plant known for its liver-nourishing properties,has shown promise in inhibiting the activation of hepatic stellate cells(HSCs),crucial indicators of hepatic fibrosis,especiall...Cornus officinalis,a medicinal and edible plant known for its liver-nourishing properties,has shown promise in inhibiting the activation of hepatic stellate cells(HSCs),crucial indicators of hepatic fibrosis,especially when processed by high pressure wine steaming(HPWS).Herein,this study aims to investigate the regulatory effects of cornus officinalis,both in its raw and HPWS forms,on inflammation and apoptosis in liver fibrosis and their underlying mechanisms.In vivo liver fibrosis models were established by subcutaneous injection of CCl4,while in vitro HSCs were exposed to transforming growth factor-b(TGF-b).These findings demonstrated that cornus officinalis with HPWS conspicuously ameliorated histopathological injury,reduced the release of proinflammatory factors,and decreased collagen deposition in CCl4-induced rats compared to its raw form.Utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer(UHPLC-QTOF-MS)combined with network analysis,we identified that the pharmacological effects of the changed components of cornus officinalis before and after HPWS,primarily centered on the adenosine phosphate(AMP)-activated protein kinase(AMPK)pathway.Of note,cornus officinalis activated AMPK and sirtuin 3(SIRT3),promoting the apoptosis of activated HSCs through the caspase cascade by regulating caspase3,caspase6 and caspase9.small interfering RNA(siRNA)experiments showed that cornus officinalis could regulate AMPK activity and its mediated-apoptosis through SIRT3.In conclusion,cornus officinalis exhibited the ability to reduce inflammation and apoptosis,with the SIRT3-AMPK signaling pathway identified as a potential mechanism underlying the synergistic effect of cornus officinalis with HPWS on anti-liver fibrosis.展开更多
[Objectives]The anti-tumor activity of fractions from Buddleja officinalis Maxim.by petroleum ether,ethyl acetate,n-butanol and water solvent was studied.[Methods]The ethanol extract from B.officinalis Maxim.was extra...[Objectives]The anti-tumor activity of fractions from Buddleja officinalis Maxim.by petroleum ether,ethyl acetate,n-butanol and water solvent was studied.[Methods]The ethanol extract from B.officinalis Maxim.was extracted and then concentrated with petroleum ether,ethyl acetate,n-butanol and water,respectively,and the extracts were obtained.The inhibitory effects of the four different fractions on the growth of three tumor cell lines in vitro were detected by CCK-8 method,and the median inhibitory concentration(IC 50 value)was calculated.[Results]The four fractions inhibited the growth of the three tumor cell lines in vitro,among which the n-butanol fraction had the best anti-tumor activity.The IC 50 values of the n-butanol fraction on human gastric cancer(SGC-7901),human breast cancer(MCF-7)and human liver cancer(BEL-7404)cell lines were 0.08,1.58 and 0.12 mg/mL,respectively.[Conclusions]Petroleum ether,ethyl acetate,n-butanol and water fractions from the ethanol extract of B.officinalis Maxim.had certain anti-tumor effects,and the n-butanol fraction had the best anti-tumor activity.展开更多
Background:Explore the anti-tumor mechanism of herb pair Pinellia ternate-Magnolia officinalis(BX-HP)in liver cancer through network pharmacology using molecular docking methods.Method:The active ingredients and corre...Background:Explore the anti-tumor mechanism of herb pair Pinellia ternate-Magnolia officinalis(BX-HP)in liver cancer through network pharmacology using molecular docking methods.Method:The active ingredients and corresponding targets of the herb pair Pinellia ternate-Magnolia officinalis were obtained from the HERB database.The relevant targets for liver cancer were obtained from GeneCards,DisGeNET,TTD,and Drugbank databases.Obtain common targets between herb pair Pinellia ternate-Magnolia officinalis and liver cancer through the Bioinformatics platform,establish a PPI network diagram using STRING software,and perform GO functional enrichment and KEGG pathway enrichment analysis on the DAVID platform.AutoDockTools 1.5.7 software and molecular dynamics simulation analysis are used to evaluate the binding of components to target proteins.HERB database,SwissTargetPrediction database,SwissADME database,UniProt database,GeneCards database,TTD database,DRUGBANK database,DisGeNET database,String,DAVID.Bioinformatics platform,PDB database,PubChem and TCMSP database.Result:A total of 22 active ingredients with a Probability>0.1 targets in Magnolia officinalis were screened,26 active ingredients with a Probability>0.1 targets in Pinellia ternata,ten vital active ingredients,corresponding to 979 and 803 targets with a Probability>0.1 targets,2536 liver cancer-related targets,and 279 targets in the herb pair Pinellia ternata-Magnolia officinalis.The GO functional enrichment analysis resulted in 1297 entries,namely 971 biological process entries,118 cell localization entries,and 208 molecular function entries.Three signaling pathways were annotated through the KEGG pathway.Based on molecular docking,ten vital active ingredients and five target proteins were validated to exhibit an excellent binding affinity.The above data indicates that combining the herb pair Pinellia ternata-Magnolia officinalis may treat liver cancer through specific targets and signaling pathways.Conclusion:Herb pair Pinellia ternata-Magnolia officinalis has a synergistic effect on treating liver cancer through multicomponent,multitarget,and multi-pathway approaches.This study provides a sufficient theoretical basis for subsequent research.展开更多
本研究以迷迭香粉粒径D50为指标,在单因素试验的基础上,采用正交试验优化迷迭香粉的冷冻超微粉碎(Freezing-superfine grinding,FSG)工艺条件,并与常温机械粉碎(Normal temperature mechanical grinding,NTMG)、常温超微粉碎(Normal tem...本研究以迷迭香粉粒径D50为指标,在单因素试验的基础上,采用正交试验优化迷迭香粉的冷冻超微粉碎(Freezing-superfine grinding,FSG)工艺条件,并与常温机械粉碎(Normal temperature mechanical grinding,NTMG)、常温超微粉碎(Normal temperature ultrafine grinding,NTUG)制备的迷迭香粉进行粒径、休止角、滑角、持油性、持水性和溶解性等理化性质的比较。结果表明,优化的FSG条件为水分含量7%、粉碎温度-18℃、粉碎时间18 min,该条件下迷迭香粉粒径D50为11.51μm。FSG制备的迷迭香粉D50显著小于NTMG和NTUG制备的迷迭香粉D50;与NTMG和NTUG相比,FSG制备的迷迭香粉休止角和滑角增加,持油性、持水性、溶解性和迷迭香酸含量明显提高。本研究为迷迭香粉的制备提供参考。展开更多
Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officina...Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officinalis were analyzed by the method of HPLC (High Performance Liquid Chromatogram). The results showed that such qualitative traits as the content of magnolol, content of honokiol, total content of key phenols, and the ratio of magnolo to honokiol differ significantly between the provenances. The provenances with a sharpened leaf tip from the western part of Hubei Province has a highest content of phenols, and that with a concave leaf tip from the Lushan Mountain has a lowest content of phenols. All these four qualitative traits were genetically controlled, with a heritability between 0.8342 and 0.9871 in terms of provenance. In addition, both longitudinal and latitudinal geographical variations could be found, with longitudinal variations being dominant. As a result, 3 superior provenances from Wufeng, Enshi and Hefeng of the western part of Hubei as well as 10 high-quality individuals were selected.展开更多
Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Cori...Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Coriandrum sativurn L.） not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers＇ income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation.展开更多
基金Supported by National Natural Science Foundation of China(82260844)Natural Science Foundation of Inner Mongolia(2022LHMS08021).
文摘[Objectives]To explore the mechanism of Mongolian medicine Valeriana officinalis L.on liver cancer by network pharmacology.[Methods]The HERB database of V.officinalis L.was searched,and the Uniprot database was used to normalize and standardize the targets.Liver cancer targets were searched through GeneCards,DISGENET,and other databases.Venny website was used to obtain the intersection target of valerian active ingredients and liver cancer disease.The protein-protein interaction(PPI)network of the intersection targets was analyzed by STRING database,and the PPI network was constructed by Cytoscape software.The David database was used for GO functional annotation and KEGG pathway enrichment analysis to obtain the relevant pathways in the treatment of liver cancer with Mongolian medicine V.officinalis.The corresponding chemical components,targets and pathways of liver cancer were imported into Cytoscape software to construct the network topology of"chemical component-disease-target-pathway".According to the analysis results,the potential of the active components in V.officinalis as a therapeutic drug for liver cancer was evaluated,and the correlation between the results of network pharmacology analysis and clinical treatment of liver cancer was discussed,which provided a reference for clinical application.[Results]A total of 13 kinds of chemical components and 108 drug disease intersection target genes were screened,and the core genes acting on diseases were caffeic acid,perillyl acetate,(+)-alpha-Terpineol,eucalyptol,etc.;GO functional enrichment analysis involved 389 items of biological processes,62 items of cellular components and 120 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out chemical carcinogenesis-receptor activation,cancer pathways,prolactin signaling pathways,proteoglycans in cancer,EGFR tyrosine kinase inhibitor resistance and other signaling pathways.[Conclusions]The mechanism of Mongolian medicine V.officinalis on liver cancer was studied by network pharmacology.It was found that it can inhibit the proliferation of liver cancer cells from multiple targets and pathways.This is expected to provide a theoretical basis for further basic experimental research.
基金Infinitus(China)Company Ltd.internal research funding。
文摘Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative action of a delay in yolk sac absorption by Bajitian was investigated by RT-PCR analysis of lipid metabolism-related microsomal triglyceride transfer protein(MTP),apolipoprotein CII(ApoC2)and lipogenesis-related liver x receptor(LXR)genes.The effect of Bajitian eliciting an inflammatory response was studied by exposing 72 hpf myeloperoxidase(MPO):GFP transgenic zebrafish embryos to Bajitian extract for 4 h.Assessment was done by TUNEL,caspase-3/7,and RT-PCR analysis of the apoptosis related pathway B-cell lymphoma 2 associated X protein(Bax),Nuclear factor kappa-lightchain-enhancer of activated B cells(NF-k B)genes,neutrophil development-related stem cell leukaemia(SCL)and transcription factor PU.1 genes,to reveal the causative action of Bajitian reducing neutrophils.Results:RT-PCR analysis found that Bajitian extract had no effect on the expression of MTP or ApoC2 genes,but upregulated LXR gene,which might explain the delay in yolk sac absorption.Analysis of the inflammatory response showed that compared with negative controls,Bajitian extract significantly(P<.05)reduced the number of neutrophils in MPO:GFP embryos.TUNEL,caspase-3/7,and RT-PCR analysis of Bax and NF-k B genes found that Bajitian extract did not trigger the cell apoptosis.Further RT-PCR analysis found that Bajitian extract did not affect SCL expression,but did lead to down-regulation of PU.1.The inhibition of neutrophil development/differentiation may explain the decline in the total number of neutrophils following Bajitian treatment,which could be attributed to the anti-inflammatory effects found clinically for this drug.Conclusions:This study demonstrated that Bajitian caused a delay in yolk sac absorption and a decrease neutrophil in zebrafish embryos,which may be related to the inhibition of neutrophil development.
基金supported by the National Key Research and Development Project(2019YFC1906601)China the Scientific and Technological Innovation Project of the Chinese Academy of Chinese Medical Sciences(C12021A04111)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ13-YQ-040).
文摘Herbal extraction residues(HERs)cause serious environmental pollution and resource waste.In this study,a novel green route was designed for the comprehensive reutilization of all components in HERs,taking Magnolia officinalis residues(MOR)as an example.The reluctant structure of MOR was first destroyed by alkali pretreatment to release the functional ingredients(magnolol and honokiol)originally remaining in MOR and to make MOR more accessible for hydrolysis.A metal–organic frame material MIL-101(Cr)with a maximum absorption capacity of 255.64 mg g^(-1)was synthesized to absorb the released honokiol and magnolol from the pretreated MOR solutions,and 40 g L^(-1)reducing sugars were obtained with 81.8%enzymatic hydrolysis rate at 10%MOR solid loading.Finally,382 mg L-1β-amyrin was produced from MOR hydrolysates by an engineered yeast strain.In total,1 kg honokiol,8 kg magnolol,and 7.64 kg β-amyrin could produce from 1 ton MOR by this cleaner process with a total economic output of 170,700 RMB.
文摘The fruit of Morinda citrifolia L., commonly known as noni, has an extensive history of use as a food and traditional medicine around the world. Adding value to Morinda citrifolia L. products, particularly the fruit, could be one way of building resilience in vulnerable farming households. The aim of this study was to determine the secondary metabolite and mineral composition of noni juice obtained by fermenting the fruit of Morinda citrifolia L. Fruits were collected in August 2022 from the local field in Thiès region, West of Senegal. Extraction yields were determined and the secondary metabolites were determined using conventional analytical methods. Calcium, magnesium, iron, sodium and potassium were determined by atomic absorption spectrophotometer coupled with a CCD detector. The results show that an average fruit mass (503.2 ± 110.96 g) consists of 171.44 ± 50.01 g pulp and 34.06 ± 10.35 g seeds. The traditional extraction yield of noni juice is 16.46% after three weeks of fermentation. The contents of total polyphenols, flavonoids and tannins obtained in noni are 608.97 ± 4.53 mg EAG/100mL, 7.78 ± 0.01 mg EQ/100mL and 0.191 ± 0.01 mg EC/100mL respectively. The ethanol content of noni varies from 3.57 to 5.23 mL/100mL during extraction. Noni has a high calcium content with a concentration of 383.79 ± 33.23 mg/L. This is followed by a good concentration of magnesium, potassium and sodium, at 278.47 ± 26.30, 187.43 ± 10.7 and 155.95 ± 28.66 mg/L respectively. Noni also has an iron content of 202.15 ± 0.05 mg/L.
基金supported by the National Natural Science Foundation of China(Grant Nos.:82104394 and 81973481)the Natural Science Foundation of Zhejiang Province(Grant Nos.:LY23H280008 and LQ23H280004)+2 种基金Zhejiang Province Traditional Chinese Medicine Science and Technology Project(Grant No.:2022ZQ033)Zhejiang Chinese Medicine University UniversityLevel Talent Special Project(Grant No.:2021ZR06)Zhejiang Province Postdoctoral Research Project(Grant No.:ZJ2022057).
文摘Cornus officinalis,a medicinal and edible plant known for its liver-nourishing properties,has shown promise in inhibiting the activation of hepatic stellate cells(HSCs),crucial indicators of hepatic fibrosis,especially when processed by high pressure wine steaming(HPWS).Herein,this study aims to investigate the regulatory effects of cornus officinalis,both in its raw and HPWS forms,on inflammation and apoptosis in liver fibrosis and their underlying mechanisms.In vivo liver fibrosis models were established by subcutaneous injection of CCl4,while in vitro HSCs were exposed to transforming growth factor-b(TGF-b).These findings demonstrated that cornus officinalis with HPWS conspicuously ameliorated histopathological injury,reduced the release of proinflammatory factors,and decreased collagen deposition in CCl4-induced rats compared to its raw form.Utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer(UHPLC-QTOF-MS)combined with network analysis,we identified that the pharmacological effects of the changed components of cornus officinalis before and after HPWS,primarily centered on the adenosine phosphate(AMP)-activated protein kinase(AMPK)pathway.Of note,cornus officinalis activated AMPK and sirtuin 3(SIRT3),promoting the apoptosis of activated HSCs through the caspase cascade by regulating caspase3,caspase6 and caspase9.small interfering RNA(siRNA)experiments showed that cornus officinalis could regulate AMPK activity and its mediated-apoptosis through SIRT3.In conclusion,cornus officinalis exhibited the ability to reduce inflammation and apoptosis,with the SIRT3-AMPK signaling pathway identified as a potential mechanism underlying the synergistic effect of cornus officinalis with HPWS on anti-liver fibrosis.
基金Supported by Guangxi Key R&D Project(GuiKeAB18221095)Baise Scientific Research and Technology Development Plan of Baise City(BaiKe20211810)+1 种基金Open Project of Scientific Research in Guangxi Key Laboratory of Molecular Pathology of Hepatobiliary Diseases(GXZDSYS-005)Research Project of High-level Talents in Youjiang Medical University for Nationalities(01002018079).
文摘[Objectives]The anti-tumor activity of fractions from Buddleja officinalis Maxim.by petroleum ether,ethyl acetate,n-butanol and water solvent was studied.[Methods]The ethanol extract from B.officinalis Maxim.was extracted and then concentrated with petroleum ether,ethyl acetate,n-butanol and water,respectively,and the extracts were obtained.The inhibitory effects of the four different fractions on the growth of three tumor cell lines in vitro were detected by CCK-8 method,and the median inhibitory concentration(IC 50 value)was calculated.[Results]The four fractions inhibited the growth of the three tumor cell lines in vitro,among which the n-butanol fraction had the best anti-tumor activity.The IC 50 values of the n-butanol fraction on human gastric cancer(SGC-7901),human breast cancer(MCF-7)and human liver cancer(BEL-7404)cell lines were 0.08,1.58 and 0.12 mg/mL,respectively.[Conclusions]Petroleum ether,ethyl acetate,n-butanol and water fractions from the ethanol extract of B.officinalis Maxim.had certain anti-tumor effects,and the n-butanol fraction had the best anti-tumor activity.
基金the National Natural Science Foundation of China(No.82204250)China Postdoctoral Science Foundation(No.2021M693961)+2 种基金Young and Middle-Aged Talent Project of Hubei Provincial Department of Education(No.Q20222808)Hubei University of Science and Technology Doctoral Startup Fund Project(No.BK202029)Outstanding Young and Middle-Aged Scientific and Technological Innovation Team in Colleges and Universities in Hubei Province(No.T2021022).
文摘Background:Explore the anti-tumor mechanism of herb pair Pinellia ternate-Magnolia officinalis(BX-HP)in liver cancer through network pharmacology using molecular docking methods.Method:The active ingredients and corresponding targets of the herb pair Pinellia ternate-Magnolia officinalis were obtained from the HERB database.The relevant targets for liver cancer were obtained from GeneCards,DisGeNET,TTD,and Drugbank databases.Obtain common targets between herb pair Pinellia ternate-Magnolia officinalis and liver cancer through the Bioinformatics platform,establish a PPI network diagram using STRING software,and perform GO functional enrichment and KEGG pathway enrichment analysis on the DAVID platform.AutoDockTools 1.5.7 software and molecular dynamics simulation analysis are used to evaluate the binding of components to target proteins.HERB database,SwissTargetPrediction database,SwissADME database,UniProt database,GeneCards database,TTD database,DRUGBANK database,DisGeNET database,String,DAVID.Bioinformatics platform,PDB database,PubChem and TCMSP database.Result:A total of 22 active ingredients with a Probability>0.1 targets in Magnolia officinalis were screened,26 active ingredients with a Probability>0.1 targets in Pinellia ternata,ten vital active ingredients,corresponding to 979 and 803 targets with a Probability>0.1 targets,2536 liver cancer-related targets,and 279 targets in the herb pair Pinellia ternata-Magnolia officinalis.The GO functional enrichment analysis resulted in 1297 entries,namely 971 biological process entries,118 cell localization entries,and 208 molecular function entries.Three signaling pathways were annotated through the KEGG pathway.Based on molecular docking,ten vital active ingredients and five target proteins were validated to exhibit an excellent binding affinity.The above data indicates that combining the herb pair Pinellia ternata-Magnolia officinalis may treat liver cancer through specific targets and signaling pathways.Conclusion:Herb pair Pinellia ternata-Magnolia officinalis has a synergistic effect on treating liver cancer through multicomponent,multitarget,and multi-pathway approaches.This study provides a sufficient theoretical basis for subsequent research.
文摘本研究以迷迭香粉粒径D50为指标,在单因素试验的基础上,采用正交试验优化迷迭香粉的冷冻超微粉碎(Freezing-superfine grinding,FSG)工艺条件,并与常温机械粉碎(Normal temperature mechanical grinding,NTMG)、常温超微粉碎(Normal temperature ultrafine grinding,NTUG)制备的迷迭香粉进行粒径、休止角、滑角、持油性、持水性和溶解性等理化性质的比较。结果表明,优化的FSG条件为水分含量7%、粉碎温度-18℃、粉碎时间18 min,该条件下迷迭香粉粒径D50为11.51μm。FSG制备的迷迭香粉D50显著小于NTMG和NTUG制备的迷迭香粉D50;与NTMG和NTUG相比,FSG制备的迷迭香粉休止角和滑角增加,持油性、持水性、溶解性和迷迭香酸含量明显提高。本研究为迷迭香粉的制备提供参考。
文摘Bark samples of Magnolis officinalis were collected from a 7-year-old trial plantation with 13 provenances in Jingning County of Zhejiang Province on June 25, 2000. The contents of magnolol and honokiol of M. officinalis were analyzed by the method of HPLC (High Performance Liquid Chromatogram). The results showed that such qualitative traits as the content of magnolol, content of honokiol, total content of key phenols, and the ratio of magnolo to honokiol differ significantly between the provenances. The provenances with a sharpened leaf tip from the western part of Hubei Province has a highest content of phenols, and that with a concave leaf tip from the Lushan Mountain has a lowest content of phenols. All these four qualitative traits were genetically controlled, with a heritability between 0.8342 and 0.9871 in terms of provenance. In addition, both longitudinal and latitudinal geographical variations could be found, with longitudinal variations being dominant. As a result, 3 superior provenances from Wufeng, Enshi and Hefeng of the western part of Hubei as well as 10 high-quality individuals were selected.
文摘Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Coriandrum sativurn L.） not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers＇ income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation.
