为研究饲粮添加氧化油脂和金荞麦对肉鸡肉品质和空肠黏膜miRNA表达谱的影响,试验选用1日龄健康、体重相近的882肉仔鸡公鸡240只,随机分为3组,每组8个重复,每个重复10只鸡。Ctr、Ox、Fag组分别在基础日粮中添加新鲜油脂、氧化油脂、新鲜...为研究饲粮添加氧化油脂和金荞麦对肉鸡肉品质和空肠黏膜miRNA表达谱的影响,试验选用1日龄健康、体重相近的882肉仔鸡公鸡240只,随机分为3组,每组8个重复,每个重复10只鸡。Ctr、Ox、Fag组分别在基础日粮中添加新鲜油脂、氧化油脂、新鲜油脂+2%金荞麦。试验期为42 d。结果显示:氧化油脂可显著增加胸肌24 h pH和腿肌滴水损失(P<0.01),显著降低胸肌肉色a^(*)值(P<0.05);金荞麦可显著增加腿肌肉色L^(*)和a^(*)值(P<0.05);Ctr vs Ox,Ctr vs Fag,Ox vs Fag三个比较组的差异表达miRNAs分别为25、27、24个;这些差异表达miRNAs的靶基因显著富集在肌动蛋白细胞骨架、紧密链接、mTOR和TGF-β等信号通路;根据表达丰度和表达差异分析,共筛选了17个差异表达miRNAs,它们可作为氧化油脂和金荞麦调控肠道屏障功能的候选基因,进一步研究氧化油脂和金荞麦通过表观遗传学对肠道屏障功能的影响。综上所述,饲粮添加氧化油脂和2%金荞麦均可改变肉鸡肉品质,影响空肠黏膜miRNAs的表达谱。展开更多
Dysregulation of post-transcriptional regulation of gene expression has been found to influence various human disorders. Aberrant miRNA-based regulation of gene expression has been found to be associated with differen...Dysregulation of post-transcriptional regulation of gene expression has been found to influence various human disorders. Aberrant miRNA-based regulation of gene expression has been found to be associated with different cancers, including breast cancers. Very little information is available on the effect of dysregulation of miRNA-mediated regulation on luminal B breast cancer. This study was aimed at comprehending the regulation of gene expression through miRNA in luminal B breast cancers by comprehensive analysis of miRNA and mRNA expression data together. Negatively regulated miRNA-target gene pairs were identified, and the target genes were functionally enriched to identify critical pathways associated with luminal B breast cancer. Further, the prognostic significance of these miRNAs and target gene pairs was assessed to identify genes with prognostic value in luminal B breast cancer. A total of 266 differentially expressed miRNAs and 164 dysregulated miRNA-target gene pairs were identified. Four genes, including SRP9, DSN1, RACGAP1, and SLC10A6, and one miRNA, hsa-mir-421, showed significant influence on the prognosis of patients with luminal B breast cancer. Through additional experimental examination of these findings, a deeper comprehension of miRNA-based post-transcriptional regulation in luminal B breast tumors will be possible.展开更多
文摘为研究饲粮添加氧化油脂和金荞麦对肉鸡肉品质和空肠黏膜miRNA表达谱的影响,试验选用1日龄健康、体重相近的882肉仔鸡公鸡240只,随机分为3组,每组8个重复,每个重复10只鸡。Ctr、Ox、Fag组分别在基础日粮中添加新鲜油脂、氧化油脂、新鲜油脂+2%金荞麦。试验期为42 d。结果显示:氧化油脂可显著增加胸肌24 h pH和腿肌滴水损失(P<0.01),显著降低胸肌肉色a^(*)值(P<0.05);金荞麦可显著增加腿肌肉色L^(*)和a^(*)值(P<0.05);Ctr vs Ox,Ctr vs Fag,Ox vs Fag三个比较组的差异表达miRNAs分别为25、27、24个;这些差异表达miRNAs的靶基因显著富集在肌动蛋白细胞骨架、紧密链接、mTOR和TGF-β等信号通路;根据表达丰度和表达差异分析,共筛选了17个差异表达miRNAs,它们可作为氧化油脂和金荞麦调控肠道屏障功能的候选基因,进一步研究氧化油脂和金荞麦通过表观遗传学对肠道屏障功能的影响。综上所述,饲粮添加氧化油脂和2%金荞麦均可改变肉鸡肉品质,影响空肠黏膜miRNAs的表达谱。
文摘Dysregulation of post-transcriptional regulation of gene expression has been found to influence various human disorders. Aberrant miRNA-based regulation of gene expression has been found to be associated with different cancers, including breast cancers. Very little information is available on the effect of dysregulation of miRNA-mediated regulation on luminal B breast cancer. This study was aimed at comprehending the regulation of gene expression through miRNA in luminal B breast cancers by comprehensive analysis of miRNA and mRNA expression data together. Negatively regulated miRNA-target gene pairs were identified, and the target genes were functionally enriched to identify critical pathways associated with luminal B breast cancer. Further, the prognostic significance of these miRNAs and target gene pairs was assessed to identify genes with prognostic value in luminal B breast cancer. A total of 266 differentially expressed miRNAs and 164 dysregulated miRNA-target gene pairs were identified. Four genes, including SRP9, DSN1, RACGAP1, and SLC10A6, and one miRNA, hsa-mir-421, showed significant influence on the prognosis of patients with luminal B breast cancer. Through additional experimental examination of these findings, a deeper comprehension of miRNA-based post-transcriptional regulation in luminal B breast tumors will be possible.
