The physicochemical characteristics of Khaya senegalensis seed oil were investigated. Iodine value, saponification value, percentage free fatty acid (FFA), acid value, and peroxide value were the chemical parameters e...The physicochemical characteristics of Khaya senegalensis seed oil were investigated. Iodine value, saponification value, percentage free fatty acid (FFA), acid value, and peroxide value were the chemical parameters examined in the oil analysis, while specific gravity and refractive index were examined as physical parameters. The results showed that the acid value was 1.18 ± 0.01, the saponification value was 191.76 ± 0.01 mgKOH/gm, and the iodine value was 102.79 ± 0.01. It contained 0.59 ± 0.01 percent free fatty acid. The value of peroxide was 6.83 ± 0.01 meq/kg. The results showed that the refractive index was 1.462 ± 0.02 and the specific gravity was 0.9194 ± 0.04. The established parameters fell within the worldwide and Nigerian vegetable oil industry standards.展开更多
A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK...A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK) and biological fertilizer (Azotobacter chroococcum, Bacillus megatherium, and Bacillus circulant) as recommended dose under new sandy soils conditions. Split plot designed with four treatments (Control, (50% Mineral fertilizer (M.) + 50% Biological fertilizer (Bio.)), 100% M. and 100% Bio.) of each species. Vegetative growth, leaf area, tree biomass, stored carbon, basal area, tree volume, and in the soil both of microbial account and mineral content were determined. The experimental results showed no significant differences between studied species among the most studied parameters except for Khaya senegalensis which gave the highest significant difference in root biomass and below-stored carbon than Swietenia mahagoni. Evidently, the highest significant growth parameters were 100% mineral fertilizer followed by (50% M. + 50% Bio.) as compared with control. No significant difference between 100% M. and (50% M. + 50% Bio.) of shoot dry biomass (15.19 and 12.02 kg, respectively) and above-stored carbon (0.28 and 0.22 Mt, respectively). Microbial account and mineral content in soil were improved after cultivation of tree species compared to before planting and control, especially with 50% mineral fertilizer and 50% bio-fertilizer treatment. In conclusion, a treatment containing 50% mineral fertilizer and 50% bio-fertilizer has led to the ideal Khaya senegalensis and Swietenia mahagoni growth in sandy soil for cheaper and sustainable.展开更多
Khaya belongs to the family Meliaceae. In Nigeria the genus is represented by three species viz; K. senegalensis A. Juss.,K. grandifoliola C. DC. and K. ivorensis A. Chev. Comparative foliar anatomy of the three Khaya...Khaya belongs to the family Meliaceae. In Nigeria the genus is represented by three species viz; K. senegalensis A. Juss.,K. grandifoliola C. DC. and K. ivorensis A. Chev. Comparative foliar anatomy of the three Khaya species was carried out to identify and describe distinctive anatomical characters that could possibly be used to delimit the three taxa. Transverse section, epidermal peels and cleared leaves of these three species were made. Characteristic similarity and disparity in the tissues arrangement as well as cell inclusions were noted for description and delimitation. The three Khaya species studied had essentially the same anatomical features, e.g., venation pattern having open polygonal areoles and the veins terminals biforkated. However, there were characters that seem to be species specific, e.g., vien termination number and areole width. The leaf epidermal studies of the three species revealed similarities in stomatal type which are generally staurocytic,epidermal cells and undulating anticlinal cell walls but stomata density varied. Hexacytic stomata is only observed in the abaxial surface of K. grandifoliola which distinguished this species from the others. The leaf petiole shape of the three species are round and difficult to distinguish into adaxial and abaxial surfaces. The cuticle is striated, vascular bundles are heart shape, conjoint, concentric and amphivasal, but are different in epidermal and collenchyma cell layer numbers. The leaf transverse sections of the three Khaya species studied have conjoint, concentric and amphicribral bundles while the leaf cuticle of K. senegalensis and K. grandifoliola are striated but that of Khaya ivorensis is non-striated.展开更多
The effect of the ethanolic extract Khaya senegalensis on some biochemical parameters was tested in rabbits with normal glucose and glucose overload. Phytochemical screening after three batches of 6 rabbits race (TBD)...The effect of the ethanolic extract Khaya senegalensis on some biochemical parameters was tested in rabbits with normal glucose and glucose overload. Phytochemical screening after three batches of 6 rabbits race (TBD) were treated with the extract in oral doses of respectively 10, 25 and 50 mg/kg body weight for 30 days;three other groups receiving respectively these doses of the extract were placed under a priori temporary overload of glucose;a control group received instead distilled water treatment. Blood glucose, triglycerides and total cholesterol were measured by spectrophotometry and weight gain of the animals was performed at the beginning of the experiment and every 2 weeks during the study period. The phytochemical screening revealed the presence of polyphenolic compounds (majority) of saponins of anthracene derivatives and steroids in the extract. The test results indicate that under the dose of 50 mg/kg body weight (BW) there was a significant decrease (p < 0.05) in blood glucose after 24 hours. The extract had no effect on the lipid profile or body weight of rabbits.展开更多
Degradation of timbers in building due to microorganisms was reported to cause enormous economic loses and species of Aspergillus are among the major cause of degradation of timbers in Nigeria. This research aims at e...Degradation of timbers in building due to microorganisms was reported to cause enormous economic loses and species of Aspergillus are among the major cause of degradation of timbers in Nigeria. This research aims at evaluating the cost of implications of biodegradations of Khaya grandifoliola by Aspergiilus spp in residential buildings empirically. Decayed Khaya grandifoliola samples were collected on residential buildings to extract, cultivate and identify the Aspergillus spp present. The cultivation went through serial dilutions and inoculations on sabouraud dextrose agar in petri dishes before incubation for 72 hours at 30 ~C. The species were identified through visual and microscopic observations. Percentage rate of degradation was determined under laboratory conditions by inoculating known weights of Khaya grandifoliola with the Aspergillus and incubating in a minimal medium for 24 weeks at 30 ~C. Weight loses and spore counts were recorded at four weeks intervals. A pattern of the degradation was forecasted. Accumulative weight loss of 16% for this period was obtained. Methods and costs of repair and replacement of decayed portions were evaluated. Residential Buildings constructed of Khaya grandifoliola experienced low cost of maintenance in this region but may not be in wetter regions where the conditions could be more favorable.展开更多
<i>Khaya senegalensis</i><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> is one of the largest and most majestic ...<i>Khaya senegalensis</i><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> is one of the largest and most majestic trees in Africa. Overexploited for its precious wood and medicinal values, the natural stands of this species are in danger of extinction in Cote d’Ivoire. Its sustainable management through regeneration techniques and assessment of its degree of adaptation to the changing climate is necessary. </span><span style="font-family:Verdana;">The aim of this study is</span><span style="font-family:Verdana;"> to evaluate the effect of different environments on seedling germination and development in </span><span style="font-family:Verdana;"><i>Khaya senegalensis</i></span><span style="font-family:Verdana;">. A total of 2160 seeds from different mother plants and 540 individuals from seed germination were selected and evaluated. The trials were conducted on three sites that were distinct by their microclimate (two nurseries in Cote d’Ivoire and one greenhouse in France). Analysis of variance showed that germination and morphology parameters were not influenced by the characteristics of the mother plants used (p > 0.05), but rather by the study sites (P < 0.05). The stable and controlled greenhouse climate was more advantageous for latency time (12.66 ± 0.80 days), germination delay (16.96 96 ± 0.54 days), germination speed (19.66 ± 2.95 days), germination duration (10.83 ± 2.27 days) and germination rate (88.88 ± 7.97) with more vigorous sowing than the other two sites. The results showed in general that the higher the height of the seedlings, the thicker the diameter of the seedlings (r = 0.796) and the higher the number of leaves (r = 0.946). This savannah species is native to the arid zones of Africa, but this study highlighted its adaptive potential to changing and different climates. These results are decision support tools for the regeneration of native pioneer forest species with high agroforestry potential and socio-economic importance such as </span><span style="font-family:Verdana;"><i>Khaya senegalensis</i></span><span style="font-family:Verdana;">. This study could be extended to other species </span><span style="font-family:Verdana;">in order to</span><span style="font-family:Verdana;"> restore disturbed ecosystems.</span></span></span></span>展开更多
OBJECTIVE： The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this...OBJECTIVE： The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract. METHODS： Cells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows： nanotechnologically--atomic force microscopy （AFM） was used to determine cell stiffness; biochemically--cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically--a carrot disk assay was used to assess the anticancer property of the extract of K. senegalensis. RESULTS： Curcumin and K. senegalensis increased the cell stiffness by 2.6- and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox （glutathione level） and energy （adenosine triphosphate） status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlike the K. senegalensis extract. In addition the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk assay. CONCLUSION： Cell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of K. senegalensis exhibited antimetastatic properties on HepG2 cells.展开更多
Meliaceae plants are distinguished by the attack of the shootborer Hypsipyla grandella and also for the occurrence of limonoids, alkaloids and phenolic compounds. Such compounds extracted from leaves of Meliaceae spec...Meliaceae plants are distinguished by the attack of the shootborer Hypsipyla grandella and also for the occurrence of limonoids, alkaloids and phenolic compounds. Such compounds extracted from leaves of Meliaceae species Cedrela odorata L., Swietenia macrophylla King, Khaya senegalensis, Toona ciliata, and C. odorata grafted onto T. ciliata plants, were tested on C. odorata leaf disks to determine their effects on survival and performance of H. grandella larvae. Larval survival was assessed 2, 10 and 25 days after starting the bioassays. Leaf consumption and weight gain per larva, days to pupa and to adult stages, pupal weight and length, and moth wing appearance were assessed for larval performance. The three compunds from the four Meliaceae species and the grafted combination affected (P ≤ 0.02) larval leaf consumption, weight gain, time to pupa and to adult stages, wing development and larval survival of H. grandella. Pupa weight (P = 0.78, F = 0.72, d.f. = 18, 160) and length (P = 0.48, F = 0.98, d.f. = 18, 160) were similar regardless of the coumpound used. Limonoid reduced larval survival on the three dates of evaluation. Alkaloids decreased leaf consumption, weight gain of larvae and time needed to reach pupa and adult stages. Alkaloids from T. ciliata and phenols from C. odorata were the best coumpounds to reduce leaf consumption and weight gain. Alkaloids from the grafted plants caused 20% of H. grandella adults to form abnormal wings.展开更多
African mahogany, one of the world’s most valuable timber species is threatened by over-exploitation in natural forests and failure of plantations due to attacks by the shoot borer Hypsipyla robusta. Mixed-species pl...African mahogany, one of the world’s most valuable timber species is threatened by over-exploitation in natural forests and failure of plantations due to attacks by the shoot borer Hypsipyla robusta. Mixed-species plantations has been reported to be an effective component of integrated pest management of major pest in other crops;but there is very limited empirical data on its use for managing Hypsipyla in mahogany mixed stands in West Africa. The aim of this study was to assess the effect of mixed-species stands as management intervention, on the growth of Khaya grandifoliola and Khaya ivorensis in relation to Hypsipyla robusta attack in a 10 ha experimental plantation in the wet evergreen forest type in Ghana. Khaya grandifoliola recorded faster growth than Khaya ivorensis in this forest type though the later naturally grow in this forest type while the former is introduced from the dry forest. Two years after planting, diameter and height growth were greater in the mixed-species stand than the pure stands for K. grandifoliola and K. ivorensis. Hypsipyla damage was less in the mixed stands of both K. grandifoliola and K. ivorensis compared to the pure stands, with the 20% and 10% Khaya mixed stand recording the lowest attack in both species. It can be recommended that mixed stands of the two Khaya species at 20% or lower Khaya density might be ideal for reducing the levels of Hypsipyla attack in this type of forest.展开更多
文摘The physicochemical characteristics of Khaya senegalensis seed oil were investigated. Iodine value, saponification value, percentage free fatty acid (FFA), acid value, and peroxide value were the chemical parameters examined in the oil analysis, while specific gravity and refractive index were examined as physical parameters. The results showed that the acid value was 1.18 ± 0.01, the saponification value was 191.76 ± 0.01 mgKOH/gm, and the iodine value was 102.79 ± 0.01. It contained 0.59 ± 0.01 percent free fatty acid. The value of peroxide was 6.83 ± 0.01 meq/kg. The results showed that the refractive index was 1.462 ± 0.02 and the specific gravity was 0.9194 ± 0.04. The established parameters fell within the worldwide and Nigerian vegetable oil industry standards.
文摘A field experiment was carried out at Ismailia Research Station, Ismailia Governorate from 2020-2022 to improve the growth of Khaya senegalensis and Swietenia mahagoni by using a combination of mineral fertilizer (NPK) and biological fertilizer (Azotobacter chroococcum, Bacillus megatherium, and Bacillus circulant) as recommended dose under new sandy soils conditions. Split plot designed with four treatments (Control, (50% Mineral fertilizer (M.) + 50% Biological fertilizer (Bio.)), 100% M. and 100% Bio.) of each species. Vegetative growth, leaf area, tree biomass, stored carbon, basal area, tree volume, and in the soil both of microbial account and mineral content were determined. The experimental results showed no significant differences between studied species among the most studied parameters except for Khaya senegalensis which gave the highest significant difference in root biomass and below-stored carbon than Swietenia mahagoni. Evidently, the highest significant growth parameters were 100% mineral fertilizer followed by (50% M. + 50% Bio.) as compared with control. No significant difference between 100% M. and (50% M. + 50% Bio.) of shoot dry biomass (15.19 and 12.02 kg, respectively) and above-stored carbon (0.28 and 0.22 Mt, respectively). Microbial account and mineral content in soil were improved after cultivation of tree species compared to before planting and control, especially with 50% mineral fertilizer and 50% bio-fertilizer treatment. In conclusion, a treatment containing 50% mineral fertilizer and 50% bio-fertilizer has led to the ideal Khaya senegalensis and Swietenia mahagoni growth in sandy soil for cheaper and sustainable.
文摘Khaya belongs to the family Meliaceae. In Nigeria the genus is represented by three species viz; K. senegalensis A. Juss.,K. grandifoliola C. DC. and K. ivorensis A. Chev. Comparative foliar anatomy of the three Khaya species was carried out to identify and describe distinctive anatomical characters that could possibly be used to delimit the three taxa. Transverse section, epidermal peels and cleared leaves of these three species were made. Characteristic similarity and disparity in the tissues arrangement as well as cell inclusions were noted for description and delimitation. The three Khaya species studied had essentially the same anatomical features, e.g., venation pattern having open polygonal areoles and the veins terminals biforkated. However, there were characters that seem to be species specific, e.g., vien termination number and areole width. The leaf epidermal studies of the three species revealed similarities in stomatal type which are generally staurocytic,epidermal cells and undulating anticlinal cell walls but stomata density varied. Hexacytic stomata is only observed in the abaxial surface of K. grandifoliola which distinguished this species from the others. The leaf petiole shape of the three species are round and difficult to distinguish into adaxial and abaxial surfaces. The cuticle is striated, vascular bundles are heart shape, conjoint, concentric and amphivasal, but are different in epidermal and collenchyma cell layer numbers. The leaf transverse sections of the three Khaya species studied have conjoint, concentric and amphicribral bundles while the leaf cuticle of K. senegalensis and K. grandifoliola are striated but that of Khaya ivorensis is non-striated.
文摘The effect of the ethanolic extract Khaya senegalensis on some biochemical parameters was tested in rabbits with normal glucose and glucose overload. Phytochemical screening after three batches of 6 rabbits race (TBD) were treated with the extract in oral doses of respectively 10, 25 and 50 mg/kg body weight for 30 days;three other groups receiving respectively these doses of the extract were placed under a priori temporary overload of glucose;a control group received instead distilled water treatment. Blood glucose, triglycerides and total cholesterol were measured by spectrophotometry and weight gain of the animals was performed at the beginning of the experiment and every 2 weeks during the study period. The phytochemical screening revealed the presence of polyphenolic compounds (majority) of saponins of anthracene derivatives and steroids in the extract. The test results indicate that under the dose of 50 mg/kg body weight (BW) there was a significant decrease (p < 0.05) in blood glucose after 24 hours. The extract had no effect on the lipid profile or body weight of rabbits.
文摘Degradation of timbers in building due to microorganisms was reported to cause enormous economic loses and species of Aspergillus are among the major cause of degradation of timbers in Nigeria. This research aims at evaluating the cost of implications of biodegradations of Khaya grandifoliola by Aspergiilus spp in residential buildings empirically. Decayed Khaya grandifoliola samples were collected on residential buildings to extract, cultivate and identify the Aspergillus spp present. The cultivation went through serial dilutions and inoculations on sabouraud dextrose agar in petri dishes before incubation for 72 hours at 30 ~C. The species were identified through visual and microscopic observations. Percentage rate of degradation was determined under laboratory conditions by inoculating known weights of Khaya grandifoliola with the Aspergillus and incubating in a minimal medium for 24 weeks at 30 ~C. Weight loses and spore counts were recorded at four weeks intervals. A pattern of the degradation was forecasted. Accumulative weight loss of 16% for this period was obtained. Methods and costs of repair and replacement of decayed portions were evaluated. Residential Buildings constructed of Khaya grandifoliola experienced low cost of maintenance in this region but may not be in wetter regions where the conditions could be more favorable.
文摘<i>Khaya senegalensis</i><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> is one of the largest and most majestic trees in Africa. Overexploited for its precious wood and medicinal values, the natural stands of this species are in danger of extinction in Cote d’Ivoire. Its sustainable management through regeneration techniques and assessment of its degree of adaptation to the changing climate is necessary. </span><span style="font-family:Verdana;">The aim of this study is</span><span style="font-family:Verdana;"> to evaluate the effect of different environments on seedling germination and development in </span><span style="font-family:Verdana;"><i>Khaya senegalensis</i></span><span style="font-family:Verdana;">. A total of 2160 seeds from different mother plants and 540 individuals from seed germination were selected and evaluated. The trials were conducted on three sites that were distinct by their microclimate (two nurseries in Cote d’Ivoire and one greenhouse in France). Analysis of variance showed that germination and morphology parameters were not influenced by the characteristics of the mother plants used (p > 0.05), but rather by the study sites (P < 0.05). The stable and controlled greenhouse climate was more advantageous for latency time (12.66 ± 0.80 days), germination delay (16.96 96 ± 0.54 days), germination speed (19.66 ± 2.95 days), germination duration (10.83 ± 2.27 days) and germination rate (88.88 ± 7.97) with more vigorous sowing than the other two sites. The results showed in general that the higher the height of the seedlings, the thicker the diameter of the seedlings (r = 0.796) and the higher the number of leaves (r = 0.946). This savannah species is native to the arid zones of Africa, but this study highlighted its adaptive potential to changing and different climates. These results are decision support tools for the regeneration of native pioneer forest species with high agroforestry potential and socio-economic importance such as </span><span style="font-family:Verdana;"><i>Khaya senegalensis</i></span><span style="font-family:Verdana;">. This study could be extended to other species </span><span style="font-family:Verdana;">in order to</span><span style="font-family:Verdana;"> restore disturbed ecosystems.</span></span></span></span>
文摘OBJECTIVE： The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract. METHODS： Cells were cultured and treated with graded concentrations of test substances for 24, 48, or 72 h with provisions made for negative controls. Treated cells were assessed as follows： nanotechnologically--atomic force microscopy （AFM） was used to determine cell stiffness; biochemically--cell cytotoxicity, glutathione level and adenosine triphosphate status, caspase activation and mitochondrial toxicity were considered; and microbiologically--a carrot disk assay was used to assess the anticancer property of the extract of K. senegalensis. RESULTS： Curcumin and K. senegalensis increased the cell stiffness by 2.6- and 4.0-fold respectively, indicating their antimetastatic effects. Corresponding changes in redox （glutathione level） and energy （adenosine triphosphate） status of the cells were also demonstrated. Further mechanistic studies indicated that curcumin was not mitotoxic in HepG2 cells unlike the K. senegalensis extract. In addition the extract potently inhibited the Agrobacterium tumefaciens-induced genetic transformation based on carrot disk assay. CONCLUSION： Cell elasticity measurement data, using AFM, strongly suggested, for the first time, that both curcumin and the extract of K. senegalensis exhibited antimetastatic properties on HepG2 cells.
文摘Meliaceae plants are distinguished by the attack of the shootborer Hypsipyla grandella and also for the occurrence of limonoids, alkaloids and phenolic compounds. Such compounds extracted from leaves of Meliaceae species Cedrela odorata L., Swietenia macrophylla King, Khaya senegalensis, Toona ciliata, and C. odorata grafted onto T. ciliata plants, were tested on C. odorata leaf disks to determine their effects on survival and performance of H. grandella larvae. Larval survival was assessed 2, 10 and 25 days after starting the bioassays. Leaf consumption and weight gain per larva, days to pupa and to adult stages, pupal weight and length, and moth wing appearance were assessed for larval performance. The three compunds from the four Meliaceae species and the grafted combination affected (P ≤ 0.02) larval leaf consumption, weight gain, time to pupa and to adult stages, wing development and larval survival of H. grandella. Pupa weight (P = 0.78, F = 0.72, d.f. = 18, 160) and length (P = 0.48, F = 0.98, d.f. = 18, 160) were similar regardless of the coumpound used. Limonoid reduced larval survival on the three dates of evaluation. Alkaloids decreased leaf consumption, weight gain of larvae and time needed to reach pupa and adult stages. Alkaloids from T. ciliata and phenols from C. odorata were the best coumpounds to reduce leaf consumption and weight gain. Alkaloids from the grafted plants caused 20% of H. grandella adults to form abnormal wings.
文摘African mahogany, one of the world’s most valuable timber species is threatened by over-exploitation in natural forests and failure of plantations due to attacks by the shoot borer Hypsipyla robusta. Mixed-species plantations has been reported to be an effective component of integrated pest management of major pest in other crops;but there is very limited empirical data on its use for managing Hypsipyla in mahogany mixed stands in West Africa. The aim of this study was to assess the effect of mixed-species stands as management intervention, on the growth of Khaya grandifoliola and Khaya ivorensis in relation to Hypsipyla robusta attack in a 10 ha experimental plantation in the wet evergreen forest type in Ghana. Khaya grandifoliola recorded faster growth than Khaya ivorensis in this forest type though the later naturally grow in this forest type while the former is introduced from the dry forest. Two years after planting, diameter and height growth were greater in the mixed-species stand than the pure stands for K. grandifoliola and K. ivorensis. Hypsipyla damage was less in the mixed stands of both K. grandifoliola and K. ivorensis compared to the pure stands, with the 20% and 10% Khaya mixed stand recording the lowest attack in both species. It can be recommended that mixed stands of the two Khaya species at 20% or lower Khaya density might be ideal for reducing the levels of Hypsipyla attack in this type of forest.
