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芙蓉葵(Hibiscus moscheutos L.)有丝分裂核型分析及减数分裂观察 被引量:6
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作者 刘坤 王省芬 +4 位作者 迟吉娜 张桂寅 吴立强 李志坤 马峙英 《植物遗传资源学报》 CAS CSCD 北大核心 2009年第2期286-289,共4页
芙蓉葵是棉属的近缘植物,具有棉花需要的早熟、强结实、耐寒、抗黄萎病等优良性状。本文对芙蓉葵的细胞学特征进行了研究,为其优良性状在棉花遗传改良中的应用提供细胞遗传学资料和理论依据。利用压片法获得了分散良好、形态清晰的有丝... 芙蓉葵是棉属的近缘植物,具有棉花需要的早熟、强结实、耐寒、抗黄萎病等优良性状。本文对芙蓉葵的细胞学特征进行了研究,为其优良性状在棉花遗传改良中的应用提供细胞遗传学资料和理论依据。利用压片法获得了分散良好、形态清晰的有丝分裂中期染色体,核型分析结果表明:芙蓉葵染色体的平均长度为5.96±0.83μm,在1号染色体上发现随体;臂比>2的染色体占10.5%,最长染色体与最短染色体的比值为1.68,核型类型为2A型,核型公式为2n=2x=38=32m+6 sm(2SAT)。芙蓉葵花粉母细胞的减数分裂正常,中期Ⅰ有19个二价体(19Ⅱ)。 展开更多
关键词 芙蓉葵 染色体 有丝分裂 核型 减数分裂
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Genetic Diversity Analysis of 44 Shares of Hibiscus cannabinus L. Germplasm Resources Using ISSR Molecular Marker 被引量:4
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作者 霍光 李德芳 +2 位作者 陈安国 李建军 唐惠娟 《Agricultural Science & Technology》 CAS 2009年第3期63-67,共5页
[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular mar... [Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources. 展开更多
关键词 Kenaf( hibiscus cannabinus l. ISSR Genetic diversity
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Insecticidal Active of Hibiscus trionum L against Wolfberry Aphis
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作者 左玲霞 谢晓鹏 杨敏丽 《Plant Diseases and Pests》 CAS 2010年第6期61-63,共3页
[Objective] The contact toxicity of the extracts from Hibiscus trionum L with different solvents against wolfberry aphis was studied.[Method] The drop method was used for the determination of the insecticidal activiti... [Objective] The contact toxicity of the extracts from Hibiscus trionum L with different solvents against wolfberry aphis was studied.[Method] The drop method was used for the determination of the insecticidal activities.[Result] 95% ethanol extract displayed the higher contact toxicity on wolfberry aphis and the corrected mortality rate of wolfberry aphids increased with the increasing of the concentration and the prolongation of treating time.The corrected mortality rate in 48 h reached 97.04% under the concentration of 50.00 g/L.The regression equation of virulence was y=1.791 0x+3.750 2 and LC50 =4.986 7 g/L.The insecticidal activities of five extracts against wolfberry aphids were as follows:chloroformpetroleum etherethyl acetaten-butanol water.The corrected mortality rate of chloroform extract in 48 h was 97.04%.[Conclusion] The study provided a certain reference for further research and its development of H.trionum and its development of new types of botanical pesticide. 展开更多
关键词 hibiscus trionum l Wolfberry aphids Insecticidal activity
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Effect of Different Sowing Dates on Disease Initiation and Development of Roselle (<i>Hibiscus sabdariffa</i>L.) Leaf Spot Disease Caused by <i>Coniella musaiensis</i>Var. <i>hibisci</i>in Makurdi, Central Nigeria 被引量:1
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作者 K.D.Apeyuan A.O.Nwankiti +1 位作者 O.A.H.Oluma E.J.Ekefan 《Journal of Geoscience and Environment Protection》 2017年第11期94-101,共8页
The experiment was conducted mainly to investigate the effect of change in sowing dates on disease severity of Roselle (Hibiscus sabdariffa L) leaf spot caused by Coniella musaiensis Var hibisci, plant height, number ... The experiment was conducted mainly to investigate the effect of change in sowing dates on disease severity of Roselle (Hibiscus sabdariffa L) leaf spot caused by Coniella musaiensis Var hibisci, plant height, number of branches, number of fruits and calyx yield of Roselle (H. sabdariffa L). Five dates were selected with an interval of fourteen days (14) between the dates for two years (2003 and 2004) to determine the most appropriate time to sow the crop using two Roselle accessions;green (Acc1) and red (Acc3).The first date was determined by the establishment of the rains in the season. It was observed that change in sowing dates had significant (p = 0.05) effect on disease severity but not on plant height, number of branches, number of fruits and calyx yield. Plants sown on July 18/27 had lowest disease indices;and are thus the best and therefore recommended for cropping of Roselle. 展开更多
关键词 ACCESSION Sowing Date DISEASE Severity hibiscus sabdariffa l. Yield. Coniella musaiensis Var hibisci
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Sterilization of <i>Hibiscus rosa-sinensis</i>L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i>Direct Regeneration
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作者 Chew Tiong Dar Janna Ong Abdullah +1 位作者 Parameswari Namasivayam Siti Habsah Roowi 《American Journal of Plant Sciences》 2012年第6期791-798,共8页
This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of ... This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox- 20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium. 展开更多
关键词 hibiscus rosa-sinensis l. Murashige and Skoog Medium AXIllARY Bud Sucrose ORGANIC Ingredient Activated Charcoal
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Tissue Culture and Rapid Propagation System of Roselle(Hibiscus sabdariffa L.)
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作者 Zifan GUI Fangjun LIAO +2 位作者 Xiaoling YE Sumei HUANG Liqing WANG 《Agricultural Biotechnology》 CAS 2022年第6期74-77,共4页
[Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle... [Objectives]This study was conducted to establish a tissue culture and rapid propagation system of roselle(Hibiscus sabdariffa L.)[Methods]A tissue culture and rapid propagation experiment was carried out with roselle plants as materials to study the suitable explants,the best disinfection method,the best growth medium,the best rooting medium and the transplanting and domestication method of roselle.[Results]The lateral buds of roselle were the best explants.Sterilizing with 0.1%mercuric chloride for 7 min showed a contamination rate of 5%and achieved a survival rate of 90%.With MS as the basic medium,adding 1.0 mg/L 6-BA and 0.5 mg/L IBA could obtain the best effect of bud induction.The medium with the highest proliferation rate was MS+6-BA 0.5 mg/L+BA 0.1 mg/L.On the basis of 1/2MS,adding 0.5 mg/L NAA+0.5 mg/L IBA could make adventitious buds root fastest and most,and greatly improve the propagation coefficient.And 1∶1 perlite rock:peat soil was the best transplanting substrate,with which the transplanting survival rate reached 95%.[Conclusions]This study provides technical reference for rapid cultivation and large-scale planting of roselle. 展开更多
关键词 hibiscus sabdariffa l. Tissue culture POlYPlOID Survival ratio
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An Efficient in Vitro Plant Regeneration System from Cotyledon of Kenaf(Hibiscus cannabinus L.)
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作者 LI Hui TANG Hui-juan +2 位作者 LI Jian-jun ZHAO Li-ning LI Yu-jun 《Agricultural Science & Technology》 CAS 2022年第2期32-37,共6页
[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The... [Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf. 展开更多
关键词 hibiscus cannabinus l. COTYlEDON REGENERATION
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Study on Quality Standard of Hibiscus trionum L.
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作者 Quan MA Yang WEN +4 位作者 Yuebu HAILAI Yuan LIU Junyin LU Tao WANG Wenbing LI 《Medicinal Plant》 CAS 2023年第3期25-28,共4页
[Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.Th... [Objectives]To establish the quality standard for Hibiscus trionum L.[Methods]The moisture,total ash,acid insoluble ash and extract of H.trionum L.were determined by the method of Chinese Pharmacopoeia 2020 edition.The medicinal materials were qualitatively identified by microscopic identification and TLC,and the content of quercetin was determined by HPLC.[Results]The moisture,total ash,acid insoluble ash and extract contents of the 12 batches of samples were 7.69%-12.94%,10.44%-14.62%,1.89%-3.64%,16.56%-21.81%,microscopic characteristic was obvious,and a method for TLC and content determination of H.trionum L.was established.[Conclusions]A perfect quality standard was established,which can be used for the quality control of H.trionum L. 展开更多
关键词 hibiscus trionum l. QUERCETIN Thin layer identification Content determination
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Effect of Organic and Chemical Fertilization on the Growth and Yield of Roselle Plants Hibiscus sabdariffa L.
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作者 Zainab Sabah Lazim Sada Nacief Jasim +2 位作者 Zhala Muhammad Ahmad Qaium Abdul Karim Zahir MohamedAziz 《Journal of Agricultural Science and Technology(B)》 2014年第10期779-786,共8页
This study was carried out during the growing season 2012-2013 at the fields of Agricultural Research Services in Sulaymaniyah Govemorate, to study the effect of chicken manure, sheep manure and chemical fertilizer NP... This study was carried out during the growing season 2012-2013 at the fields of Agricultural Research Services in Sulaymaniyah Govemorate, to study the effect of chicken manure, sheep manure and chemical fertilizer NPK on growth, sepals, seeds yield and some chemical constituents of Roselle plants grown under Sulaymaniyah conditions. The experiment included several treatments as follows: sheep manure, 75% sheep + 25% chicken, 50% sheep + 50% chicken, 25% sheep + 75% chicken, entire chicken manure, chemical fertilizer NPK and unfertilized control. The obtained results showed that all treatments of organic fertilization and NPK were significantly enhanced the vegetative growth parameters, sepals and concentration of anthocyanin of Roselle plants sepals. The treatment of (25% sheep + 75% chicken) gave the best results: plant height (205.33 cm), stem diameter (33.45 ram), number of branches/plant (22.33), total leaf area (3.29 m2), root dry weight (56.81 g), number of fruits/plant (110), fresh weight of fruits (818.44 g) and dry weight of sepals (52.65 g). But the highest concentration of anthocyanin was in the treatment of (75% sheep + 25% chicken) (260.33 mg/100 g) and the lowest concentration of oxalic acid was found in the treatment of (50% sheep + 50% chicken) (4.69 mg/100 g). The results of this study showed that the use of (25% sheep + 75% chicken) could enhance the vegetative growth and produce maximum yield of RoseUe calyx. 展开更多
关键词 hibiscus sabdariffa l. chicken manure sheep manure organic fertilizers NPK fertilizer growth calyx yield.
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Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Hibiscus syriacus L.
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作者 Haibo DENG Bei NING +3 位作者 Shunfan ZHU Chenzhong JIN Yihong HU Xuejiao ZHANG 《Agricultural Biotechnology》 CAS 2018年第3期161-164,共4页
The ultrasonic-assisted extraction of polysaccharides from Hibiscus syriacus L. at 180 W low power was optimized by the single factor tests and orthogonal test. Extraction temperature,ultrasonic treatment time,extract... The ultrasonic-assisted extraction of polysaccharides from Hibiscus syriacus L. at 180 W low power was optimized by the single factor tests and orthogonal test. Extraction temperature,ultrasonic treatment time,extraction times,and material-to-liquid ratio were considered as independent variables. The results suggested that the best extraction condition were determined as the extraction temperature at 80 ℃,the ultrasonic treatment time of 40 min,the material-to-liquid ratio at1∶ 20( g/ml),and the extraction times of 3. Under the optimal conditions,the extraction rate of polysaccharides from Hibiscus syriacus was 3. 114%. 展开更多
关键词 hibiscus syriacus l. POlYSACCHARIDE Ultrasound-assisted extraction
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Comparative Effect of Foliar Application of Cow Dung, Wood Ash and Benlate on the Disease Initiation and Development of Roselle (Hibiscus sabdariffa L.) Leaf Spot Disease Caused by Coniella musaiensis Var. Hibisci. in Makurdi, Central Nigeria
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作者 K. D. Apeyuan A. O. Nwankiti +1 位作者 H. O. A. Oluma E. J. Ekefan 《Journal of Geoscience and Environment Protection》 2016年第8期26-32,共7页
A study on the comparative effect of cow dung, wood ash and benlate for the control of leaf spot disease of Roselle (Hibiscus sabdariffa L.) was carried out following the preparation and foliar application of the mate... A study on the comparative effect of cow dung, wood ash and benlate for the control of leaf spot disease of Roselle (Hibiscus sabdariffa L.) was carried out following the preparation and foliar application of the materials on selected Roselle plants of the green and red accessions (Acc<sub>1</sub> & Acc<sub>3</sub>) in the field. The cow dung was fermented for 14 days while sour milk was added to wood ash, dissolved and all filtered and labeled. The spraying commenced soon after the plants were thinned to one and was done weekly until after 50% of the plants had flowered. Results showed significant (p = 0.05) difference between benlate, the synthetic fungicide and the bio-fertilizers (cow dung and wood ash) for the two years of the study. Disease severity was significantly lower in the benlate than in the bio-fertilizers. There was no significant difference in yield between the treatments although higher yield was recorded in benlate. Though the bio-fertilizers could not suppress infection, they aided the growth of the plants by boosting luxuriant growth. The result showed that these bio-fertilizers cannot be used as substitute to the synthetic fungicide for the control of this leaf spot disease caused by Coniella musaiensis var. hibisci. However, since they give equally good yield the bio-fertilizers can still be used to minimize the reliance upon use of chemical fungicides. 展开更多
关键词 Foliar Application Cow Dung Wood Ash Benlate Disease Initiation hibiscus sabdariffa l. ACCESSION leaf Spot Coniella musaiensis Var. Hibisci
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NaCl胁迫对芙蓉葵和黄秋葵种子萌发的影响 被引量:15
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作者 郭艳超 孙昌禹 +3 位作者 王文成 吴新海 董文琦 马立军 《河北农业科学》 2011年第8期10-14,共5页
在实验室控制环境因子条件下,用0(蒸馏水,CK)、0.2%、0.4%、0.6%、0.8%和1.0%计6个浓度的NaCl溶液处理芙蓉葵和黄秋葵种子后进行萌发试验,研究了不同盐度胁迫下种子发芽率、发芽速度、活力指数和相对干重含水量的变化。结果表明:低浓度... 在实验室控制环境因子条件下,用0(蒸馏水,CK)、0.2%、0.4%、0.6%、0.8%和1.0%计6个浓度的NaCl溶液处理芙蓉葵和黄秋葵种子后进行萌发试验,研究了不同盐度胁迫下种子发芽率、发芽速度、活力指数和相对干重含水量的变化。结果表明:低浓度盐胁迫对芙蓉葵种子萌发有促进作用,而对黄秋葵有轻度抑制作用;当盐胁迫浓度超过0.8%时,2种植物种子的萌发均受到不良影响,尤其是黄秋葵受到影响较大。2种植物均能在轻度盐渍化生境中生长,可作为耐盐碱园林植物在盐碱地试栽应用,其中芙蓉葵可在河北省大部分盐碱地区进行广泛种植。 展开更多
关键词 芙蓉葵 黄秋葵 盐胁迫 种子萌发 耐盐性
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Impacts of different drying strategies on drying characteristics,the retention of bio-active ingredient and colour changes of dried Roselle 被引量:7
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作者 Thing Chai Tham Mei Xiang Ng +6 位作者 Shu Hui Gan Lee Suan Chua Ramlan Aziz Luqman Chuah Abdullah Sze Pheng Ong Nyuk Ling Chin Chung Lim Law 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2018年第2期303-316,共14页
The drying kinetics of Roselle (Hibiscus sabdariffa L) of variety Terengganu (UMKL-1) and the quality attribution of Roselle were studied. The experiments were conducted using four different drying methods, includ... The drying kinetics of Roselle (Hibiscus sabdariffa L) of variety Terengganu (UMKL-1) and the quality attribution of Roselle were studied. The experiments were conducted using four different drying methods, including solar greenhouse drying (SD), solar greenhouse with intermittent heat pump drying (SIHP), hot air drying (HA) and heat pump drying (HP). Among the four drying methods, HP achieved the highest drying rate at a range from 0.054 g H20-(g DM)-1. rain-1 to 0.212 g H20-(g DM)-1. rain 1 while SD had the lowest drying rate, measured at 0.042 g H2O. (g DM) 1.min- 1. The analysis on colour kinetics revealed that there is no significant colour loss (p 〉 0.05) observed from HP's dried Roselle. Greater amount of flavonoid compounds i.e. protocatechuic acid was found in SD and SIHP dried finished product whereas HP's dried Roselle contains higher percentage of catechin as compared to other drying methods. 展开更多
关键词 hibiscus sabdariffa l.Drying Heat pump Total colour change Protocatechuic acid Catechin
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Effect of Roselle calyces extract on the chemical and sensory properties of functional cupcakes 被引量:1
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作者 A.R.Abdel-Moemin 《Food Science and Human Wellness》 SCIE 2016年第4期230-237,共8页
Roselle calyces(RC)are a major crop for export and used to make a common drink in Egypt.Dried RC are commercially available and appreciated to obtain concentrated extracts which might be used in the food and pharmaceu... Roselle calyces(RC)are a major crop for export and used to make a common drink in Egypt.Dried RC are commercially available and appreciated to obtain concentrated extracts which might be used in the food and pharmaceutical industries for color and heath benefits.The objective of this research was to determine the chemical and the sensory properties of cupcakes formulated with Roselle calyces extract(RCE).Proximate analysis,anthocyanins,ascorbic acid,titrable acidity,%retaining of anthocyanins,color and sensory evaluations were done.RC cupcakes had high sensory scores(P<0.05)compared to control cupcakes.The parameter a*was significantly red in the RC cupcakes compared to control cupcakes along with 77%retaining of anthocyanins.The consumption of 100 g of the RC cupcakes would provide 465 mg/100 g dry matter anthocyanin that is more than 2 folds of the minimum average of the daily intake of anthocyanins for Americans,along∼1/3 of the daily dietary fiber intake to achieve fiber adequacy according to the Scientific Advisory Committee on Nutrition.RC cupcake can be a functional food and would have a“clean”label with cost effective advantage. 展开更多
关键词 Roselle calyces hibiscus sabdariffa l. Anthocyanins Cupcakes FIBER Cost effective product
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实生苗嫁接对红麻细胞质雄性不育系与保持系的生长发育及越冬抗寒性的影响 被引量:6
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作者 黄其椿 赵洪涛 +4 位作者 刘吉敏 周瑞阳 赵艳红 王杰 李初英 《华中农业大学学报》 CAS CSCD 北大核心 2013年第3期8-14,共7页
为使红麻细胞质雄性不育系和保持系在华南地区安全越冬,实现多年生栽培,将不育系的繁殖固定于较低的回交世代,以抗寒性较强的红麻野生种H040、P3A×H040杂交种为砧木,P3A、P3B的实生苗为接穗,并以本砧嫁接为对照,探讨嫁接对红麻雄... 为使红麻细胞质雄性不育系和保持系在华南地区安全越冬,实现多年生栽培,将不育系的繁殖固定于较低的回交世代,以抗寒性较强的红麻野生种H040、P3A×H040杂交种为砧木,P3A、P3B的实生苗为接穗,并以本砧嫁接为对照,探讨嫁接对红麻雄性不育系和保持系的生长发育及越冬抗寒性的效应。聚类和隶属函数分析结果显示:抗寒性强弱可划分为5类,红麻野生种H040和杂交组合P3A×H040抗寒性最强,为1级;P3A/P3A×H040和P3A/H040抗寒性较强,2级;P3A CK和P3A/P3B CK抗寒性中等,3级;P3B/P3A×H040和P3B/H040抗寒性较弱,4级;P3B/P3A和P3B CK抗寒性最弱,5级。以抗寒性较强的实生苗为砧木嫁接可提高红麻接穗的抗寒性。 展开更多
关键词 红麻(hibiscus cannabilus l.) 细胞质雄性不育 嫁接 抗寒性
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不同浓度Cd、Pb对红麻抗逆生理特性的影响 被引量:5
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作者 李正文 李兰平 +2 位作者 周琼 李志刚 周瑞阳 《湖北农业科学》 北大核心 2013年第15期3568-3571,共4页
以红麻(Hibiscus cannabinus L.)细胞质雄性不育系P3A、恢复系992及杂交种F1为材料,采用桶栽试验研究不同浓度镉(Cd)、铅(Pb)胁迫下红麻生理生化特性的变化。结果表明,在Cd、Pb胁迫下3个红麻材料的丙二醛(MDA)、脯氨酸(Pro)含量与Cd、P... 以红麻(Hibiscus cannabinus L.)细胞质雄性不育系P3A、恢复系992及杂交种F1为材料,采用桶栽试验研究不同浓度镉(Cd)、铅(Pb)胁迫下红麻生理生化特性的变化。结果表明,在Cd、Pb胁迫下3个红麻材料的丙二醛(MDA)、脯氨酸(Pro)含量与Cd、Pb处理浓度呈正相关,即随Cd、Pb处理浓度的升高而升高,处理最高浓度时MDA、Pro含量达到最大;过氧化物酶(POD)活性与对照相比总体呈下降趋势;超氧化物歧化酶(SOD)活性在Cd、Pb处理下先上升后下降。所有生理指标变化趋势中以P3A变化最明显;F1变化最不明显,表现出明显的杂种优势。 展开更多
关键词 红麻(hibiscus cannabinus l ) 生理特性
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红麻下胚轴原生质体的分离与培养 被引量:6
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作者 牛英 刘恒蔚 +1 位作者 周瑞阳 田志宏 《湖北农业科学》 北大核心 2006年第2期149-152,共4页
采用暗培养3日龄的红麻无菌苗下胚轴,在0.45mol.L-1甘露醇的渗透压条件下,以5.0mg.L-1纤维素酶(CelluloseR-10)和5.0mg.L-1果胶酶(Pectinase)酶解12h,原生质体的产率最高,活力较强。以5.0×104个.mL-1的密度,在改良的KM8P培养基中,... 采用暗培养3日龄的红麻无菌苗下胚轴,在0.45mol.L-1甘露醇的渗透压条件下,以5.0mg.L-1纤维素酶(CelluloseR-10)和5.0mg.L-1果胶酶(Pectinase)酶解12h,原生质体的产率最高,活力较强。以5.0×104个.mL-1的密度,在改良的KM8P培养基中,附加0.5mg.L-12,4-D和0.5mg.L-16-BA,用微滴法培养,对原生质体的分裂和愈伤组织的形成极为有利。 展开更多
关键词 红麻(hibiscus canabius l) 下胚轴 原生质体 分离 培养
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施氮和耕作方式对红麻福红992农艺性状和产量的影响 被引量:1
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作者 宋岩 王道波 刘永贤 《湖北农业科学》 2015年第1期70-72,77,共4页
以红麻(Hibiscus cannabinus L.)的常规栽培品种福红992为例,探讨不同施氮和耕作方式对红麻生长发育和产量形成的影响,为红麻高产高效栽培提供合理的施肥和耕作依据。通过大田试验,研究了施氮和耕作方式对福红992农艺性状和干物质积累... 以红麻(Hibiscus cannabinus L.)的常规栽培品种福红992为例,探讨不同施氮和耕作方式对红麻生长发育和产量形成的影响,为红麻高产高效栽培提供合理的施肥和耕作依据。通过大田试验,研究了施氮和耕作方式对福红992农艺性状和干物质积累的影响,试验设3种耕作方式,即免耕、垄耕和深松耕(粉垄);3种施氮方式,即T1(20%作基肥,80%作追肥),T2(40%作基肥,60%作追肥)和T3(60%作基肥,40%作追肥)。结果表明,与免耕相比,深松耕的生麻产量、种子产量和地上部干质量分别增加15.1%、14.9%和14.5%,差异显著;与T1相比,T2生麻产量、种子产量和地上部干质量分别增加9.9%、30.3%和15.1%,差异显著;免耕和垄耕时,T2株高、皮厚、茎粗和生麻产量、种子产量、地上部干质量均达到最大值;深松耕时,T1株高、皮厚、茎粗和生麻产量、种子产量、地上部干质量均达到最大值。研究表明,深松耕T1时,生麻产量和种子产量同时达到最高。 展开更多
关键词 红麻(hibiscus cannabinus l.) 耕作方式 施氮方式 生麻产量 种子产量 农艺性状
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多年生宿根花卉芙蓉葵耐盐性研究 被引量:5
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作者 王文成 孙昌禹 +4 位作者 孙宇 郭艳超 薛志忠 李克晔 封晓辉 《河北农业科学》 2013年第6期22-27,共6页
为了给芙蓉葵在滨海盐渍地播种育苗和重盐碱地园林绿化应用提供科学依据,分别以芙蓉葵种子、幼苗和1 a生越冬根为试材,在人工模拟盐胁迫强度条件下,研究了不同浓度NaCl溶液处理对芙蓉葵种子发芽期和苗期耐盐性,以及越冬根萌生苗耐盐性... 为了给芙蓉葵在滨海盐渍地播种育苗和重盐碱地园林绿化应用提供科学依据,分别以芙蓉葵种子、幼苗和1 a生越冬根为试材,在人工模拟盐胁迫强度条件下,研究了不同浓度NaCl溶液处理对芙蓉葵种子发芽期和苗期耐盐性,以及越冬根萌生苗耐盐性的影响。结果表明:0.2%盐度胁迫会促进芙蓉葵种子萌发,随着NaCl浓度的增大,种子初始萌发时间延长,发芽率和萌发初期生长均受到抑制,且抑制程度均随盐胁迫浓度的增大而逐渐增强,盐度>0.6%后会显著抑制种子萌发,但每天给予12 h光照可以显著提高发芽率;盐胁迫会抑制芙蓉葵幼苗生长,且抑制作用随盐胁迫浓度的增大而逐渐增强,不影响其正常形态性状的最高盐胁迫浓度为0.6%,当盐胁迫浓度超过该阈值后,会使芙蓉葵幼苗的观赏价值明显降低;芙蓉葵越冬根发芽期的耐盐能力较强,盐度>1.2%后成活率显著下降。根据生理指标测定和幼苗外观形态观测结果,推断芙蓉葵种子萌发和幼苗生长的耐盐阈值是0.6%,越冬根正常萌发的耐盐阈值是1.2%。 展开更多
关键词 芙蓉葵 盐胁迫 发芽率 越冬根 形态指标
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芙蓉葵的黄萎病抗性鉴定
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作者 李志坤 刘坤 +4 位作者 张艳 张桂寅 吴立强 王省芬 马峙英 《农学学报》 2012年第3期13-16,共4页
为挖掘抗棉花黄萎病菌新种质,进而为棉花抗黄萎病育种提供有益基因资源。以芙蓉葵(Hibiscus moscheutos L.)、‘中棉所8号’和Pima90-53为材料,采用蛭石育苗和无菌育苗2种方法,对其黄萎病抗性进行了鉴定。结果表明,采用蛭石育苗,芙蓉葵... 为挖掘抗棉花黄萎病菌新种质,进而为棉花抗黄萎病育种提供有益基因资源。以芙蓉葵(Hibiscus moscheutos L.)、‘中棉所8号’和Pima90-53为材料,采用蛭石育苗和无菌育苗2种方法,对其黄萎病抗性进行了鉴定。结果表明,采用蛭石育苗,芙蓉葵表现出高抗黄萎病,病情指数为7.69,其抗性优于抗病的海岛棉品种‘Pima90-53’。陆地棉品种‘中棉所8号’表现出感病性状。采用无菌育苗,芙蓉葵黄萎病抗性优于海岛棉品种‘Pima90-53’。2种抗性鉴定方法中,均不能从接菌培养的芙蓉葵分离出黄萎病菌。说明芙蓉葵具有高抗棉花黄萎病的特性。 展开更多
关键词 芙蓉葵 陆地棉 海岛棉 黄萎病 抗性鉴定
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