Objective The neuroprotective function of heat shock protein A5(HSPA5)in ischemic stroke has been confirmed.This study aimed to investigate the effects of early aerobic exercise on neurological function recovery from ...Objective The neuroprotective function of heat shock protein A5(HSPA5)in ischemic stroke has been confirmed.This study aimed to investigate the effects of early aerobic exercise on neurological function recovery from cerebral ischemia/reperfusion and to determine whether these effects are associated with the expression level of HSPA5 in the ischemic penumbra.Methods A total of 72 male Sprague-Dawley rats were randomly assigned to the ischemia and exercise group[middle cerebral artery occlusion(MCAO)-Ex,n=18],ischemia and sedentary group(MCAO-St,n=18),sham-surgery and exercise group(Sham-Ex,n=18),or sham-surgery and sedentary group(Sham-St,n=18).The MCAO-Ex and MCAO-St groups were subjected to MCAO for 60 min,whereas the Sham-Ex and Sham-St groups were subjected to an identical operation without MCAO.Rats in the MCAO-Ex and Sham-Ex groups then ran on a treadmill for 30 min once a day for 5 consecutive days.After reperfusion,the motor function of the rats was scored by the Bederson neurological function test,balance beam test,and screen test.Nissl staining was conducted to assess morphological and structural change of nerve cells in the ischemic penumbra.The reverse transcription-quantitative polymerase chain reaction was applied to detect the mRNA expression of HSPA5.Western blot analysis was conducted to determine the protein expression of HSPA5.Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL)staining was carried out in the ischemic penumbra after MCAO.Results Rats receiving early treadmill exercise had lower Bederson neurological function,balance beam,and screen test scores on the 3rd,7th,and 14th days after MCAO;in addition,more neurons survived in the ischemic penumbra after MCAO,and higher mRNA and protein expression of HSPA5 and fewer TUNEL-positive stained cells were observed.Conclusion Our study demonstrated that early aerobic exercise can improve neurological function recovery after ischemia/reperfusion.Furthermore,the increased level of HSPA5 in the ischemic penumbra might be one of the mechanisms of enhanced neurological function recovery.展开更多
目的:探讨热休克蛋白5(heat shock protein family A member 5,HSPA5)对青蒿琥酯诱导人肝癌SMMC-7721细胞株化疗敏感性的影响。方法:用不同浓度青蒿琥酯处理SMMC-7721细胞,CCK-8法检测细胞活性,筛选最佳实验浓度。将SMMC-7721细胞按以...目的:探讨热休克蛋白5(heat shock protein family A member 5,HSPA5)对青蒿琥酯诱导人肝癌SMMC-7721细胞株化疗敏感性的影响。方法:用不同浓度青蒿琥酯处理SMMC-7721细胞,CCK-8法检测细胞活性,筛选最佳实验浓度。将SMMC-7721细胞按以下分组处理:对照组、青蒿琥酯组、青蒿琥酯+去铁胺组,用流式细胞术检测细胞内脂质来源活性氧水平;试剂盒检测细胞内丙二醛水平。用包装HSPA5干扰或过表达质粒的慢病毒感染SMMC-7721细胞,qRT-PCR和蛋白质印迹法分别测定转染后HSPA5 mRNA和蛋白表达;CCK-8法检测细胞活性,试剂盒检测细胞内丙二醛水平。结果:青蒿琥酯浓度为20μmol/L时,SMMC-7721细胞达到半数致死量,为最佳实验浓度;流式细胞术结果显示,青蒿琥酯处理的细胞内脂质来源活性氧水平、丙二醛水平明显升高,去铁胺可抑制青蒿琥酯导致的细胞内脂质来源活性氧和丙二醛升高(均P <0. 05);经过青蒿琥酯处理的HSPA5干扰组细胞活性水平明显低于未干扰组,丙二醛水平明显高于未干扰组(均P <0. 05)。结论:干扰HSPA5可能增强人肝癌SMMC-7721细胞株对青蒿琥酯的化疗敏感性。展开更多
基金This study was funded by Basic Research Projects in Shanxi Province(No.2015021178)Start-up Funds for Doctoral Research at Shanxi Datong University(No.2014-B-01).
文摘Objective The neuroprotective function of heat shock protein A5(HSPA5)in ischemic stroke has been confirmed.This study aimed to investigate the effects of early aerobic exercise on neurological function recovery from cerebral ischemia/reperfusion and to determine whether these effects are associated with the expression level of HSPA5 in the ischemic penumbra.Methods A total of 72 male Sprague-Dawley rats were randomly assigned to the ischemia and exercise group[middle cerebral artery occlusion(MCAO)-Ex,n=18],ischemia and sedentary group(MCAO-St,n=18),sham-surgery and exercise group(Sham-Ex,n=18),or sham-surgery and sedentary group(Sham-St,n=18).The MCAO-Ex and MCAO-St groups were subjected to MCAO for 60 min,whereas the Sham-Ex and Sham-St groups were subjected to an identical operation without MCAO.Rats in the MCAO-Ex and Sham-Ex groups then ran on a treadmill for 30 min once a day for 5 consecutive days.After reperfusion,the motor function of the rats was scored by the Bederson neurological function test,balance beam test,and screen test.Nissl staining was conducted to assess morphological and structural change of nerve cells in the ischemic penumbra.The reverse transcription-quantitative polymerase chain reaction was applied to detect the mRNA expression of HSPA5.Western blot analysis was conducted to determine the protein expression of HSPA5.Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL)staining was carried out in the ischemic penumbra after MCAO.Results Rats receiving early treadmill exercise had lower Bederson neurological function,balance beam,and screen test scores on the 3rd,7th,and 14th days after MCAO;in addition,more neurons survived in the ischemic penumbra after MCAO,and higher mRNA and protein expression of HSPA5 and fewer TUNEL-positive stained cells were observed.Conclusion Our study demonstrated that early aerobic exercise can improve neurological function recovery after ischemia/reperfusion.Furthermore,the increased level of HSPA5 in the ischemic penumbra might be one of the mechanisms of enhanced neurological function recovery.
文摘目的:探讨热休克蛋白5(heat shock protein family A member 5,HSPA5)对青蒿琥酯诱导人肝癌SMMC-7721细胞株化疗敏感性的影响。方法:用不同浓度青蒿琥酯处理SMMC-7721细胞,CCK-8法检测细胞活性,筛选最佳实验浓度。将SMMC-7721细胞按以下分组处理:对照组、青蒿琥酯组、青蒿琥酯+去铁胺组,用流式细胞术检测细胞内脂质来源活性氧水平;试剂盒检测细胞内丙二醛水平。用包装HSPA5干扰或过表达质粒的慢病毒感染SMMC-7721细胞,qRT-PCR和蛋白质印迹法分别测定转染后HSPA5 mRNA和蛋白表达;CCK-8法检测细胞活性,试剂盒检测细胞内丙二醛水平。结果:青蒿琥酯浓度为20μmol/L时,SMMC-7721细胞达到半数致死量,为最佳实验浓度;流式细胞术结果显示,青蒿琥酯处理的细胞内脂质来源活性氧水平、丙二醛水平明显升高,去铁胺可抑制青蒿琥酯导致的细胞内脂质来源活性氧和丙二醛升高(均P <0. 05);经过青蒿琥酯处理的HSPA5干扰组细胞活性水平明显低于未干扰组,丙二醛水平明显高于未干扰组(均P <0. 05)。结论:干扰HSPA5可能增强人肝癌SMMC-7721细胞株对青蒿琥酯的化疗敏感性。
