HLA-C,HLA-DP and HLA-DQ are thought to be benign due to low expression and few initial negative studies.Historically,most allocation programs used HLA-A,HLA-B and HLA-DR antigens for matching.With the advent and use o...HLA-C,HLA-DP and HLA-DQ are thought to be benign due to low expression and few initial negative studies.Historically,most allocation programs used HLA-A,HLA-B and HLA-DR antigens for matching.With the advent and use of single-bead antigen assays,more was learned about donor-specific antibodies(DSAs)against these antigens.Interest in these antigens and antibodies grew when cases of acute antibody-mediated rejection(AMR),mixed rejections,chronic AMR,and reduced graft survival were reported with DSAs against these antigens.Although the deleterious effects of these DSAs are more pronounced in retransplants,harmful effects have also been observed in first-time recipients.DSAs against each of these antigens can trigger rejection alone.Their combination with DSAs against HLA-A,HLA-B and HLA-DR can cause more damage.It has been shown that strategies that reduce mismatches for these antigen lead to fewer rejections and better graft survival.There is a need for greater consensus on the universal typing of these antigens prior to transplantation for better patient and graft outcomes.This review focuses on the interaction of these antigens with lymphocytes and killer immunoglobulin receptors,arguments for not typing them,detailed analyses of the literature about their harmful effects,potential strategies moving forward,and recommendations for the future.展开更多
目的:研究HLA-C(histocompatibility complex class C)和PDCD1(programmed cell death 1)在银屑病患者骨髓CD34^+细胞中的表达,以揭示银屑病患者造血干细胞的活性。方法:采用免疫磁珠法分离CD34^+细胞,RT-PCR法分别检测HLA-C、PDCD1的m...目的:研究HLA-C(histocompatibility complex class C)和PDCD1(programmed cell death 1)在银屑病患者骨髓CD34^+细胞中的表达,以揭示银屑病患者造血干细胞的活性。方法:采用免疫磁珠法分离CD34^+细胞,RT-PCR法分别检测HLA-C、PDCD1的mRNA表达。结果:银屑病患者骨髓CD34^+细胞HLA-CmRNA表达水平高于正常对照组(P<0.05),PDCD1 mRNA表达在银屑病组与正常对照组间比较无统计学意义(P>0.05),未发现HLA-C mRNA表达水平与银屑病皮损面积和疾病活动性指数(PASI)评分有直线相关性。结论:影响CD34^+细胞发育分化的某些基因在银屑病患者骨髓CD34^+细胞中表达异常,这些基因表达水平的异常可能引起骨髓造血干细胞活性异常,并参与银屑病的发病。展开更多
文摘HLA-C,HLA-DP and HLA-DQ are thought to be benign due to low expression and few initial negative studies.Historically,most allocation programs used HLA-A,HLA-B and HLA-DR antigens for matching.With the advent and use of single-bead antigen assays,more was learned about donor-specific antibodies(DSAs)against these antigens.Interest in these antigens and antibodies grew when cases of acute antibody-mediated rejection(AMR),mixed rejections,chronic AMR,and reduced graft survival were reported with DSAs against these antigens.Although the deleterious effects of these DSAs are more pronounced in retransplants,harmful effects have also been observed in first-time recipients.DSAs against each of these antigens can trigger rejection alone.Their combination with DSAs against HLA-A,HLA-B and HLA-DR can cause more damage.It has been shown that strategies that reduce mismatches for these antigen lead to fewer rejections and better graft survival.There is a need for greater consensus on the universal typing of these antigens prior to transplantation for better patient and graft outcomes.This review focuses on the interaction of these antigens with lymphocytes and killer immunoglobulin receptors,arguments for not typing them,detailed analyses of the literature about their harmful effects,potential strategies moving forward,and recommendations for the future.
文摘目的:研究HLA-C(histocompatibility complex class C)和PDCD1(programmed cell death 1)在银屑病患者骨髓CD34^+细胞中的表达,以揭示银屑病患者造血干细胞的活性。方法:采用免疫磁珠法分离CD34^+细胞,RT-PCR法分别检测HLA-C、PDCD1的mRNA表达。结果:银屑病患者骨髓CD34^+细胞HLA-CmRNA表达水平高于正常对照组(P<0.05),PDCD1 mRNA表达在银屑病组与正常对照组间比较无统计学意义(P>0.05),未发现HLA-C mRNA表达水平与银屑病皮损面积和疾病活动性指数(PASI)评分有直线相关性。结论:影响CD34^+细胞发育分化的某些基因在银屑病患者骨髓CD34^+细胞中表达异常,这些基因表达水平的异常可能引起骨髓造血干细胞活性异常,并参与银屑病的发病。
