<span style="font-family:Verdana;">Backgrou</span><span style="font-family:Verdana;">nd/Aim: Kuji amber is an interesting natural source for drug discovery</span><span>...<span style="font-family:Verdana;">Backgrou</span><span style="font-family:Verdana;">nd/Aim: Kuji amber is an interesting natural source for drug discovery</span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">because a new anti-allergic compound, named kujigamberol and several new compounds have been isolatated from it. It was important to evaluate the yield, biological activities and constituents of each methanol extract</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">of Kuji, Iwaki, Choshi, Mizunami and Ube ambers in Japan in order to establish if additional new compounds could be identified in these ambers. Materia</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">ls and </span><span style="font-family:Verdana;">Method: Biological activities of each extract were evaluated using growth-restoring </span><span style="font-family:Verdana;">activity of the mutant yeast strain involving Ca</span><sup><span style="font-family:Verdana;">2+</span></sup><span style="font-family:Verdana;">-signal transduction and inhibi</span><span style="font-family:Verdana;">tion activity of degranulation in rat basophilic leukemia (RBL)-2H3 cells. Constituents</span><span style="font-family:Verdana;"> of each extract were analyzed by high performance liquid chromatography (HPLC). Results: All ambers except Ube amber have growth-restoring activity against the mutant yeast. Both Kuji and Iwaki ambers inhibited the degranulation of RBL-2H3 cells induced by the calcium ionophore A23187 in a dose dependent manner. The main biologically active compound in Kuji amber, kujigamberol, was also isolated from Iwaki amber and analyzed by mass spectrometry (MS) and nuclear magnetic resonance (NMR). Conclusion: Kuji and Iwaki ambers appeared to have the same origin. Choshi, Mizunami, and Ube ambers are valuable sources for biologically active compounds which are different from those of Kuji amber.</span></span></span></span>展开更多
文摘<span style="font-family:Verdana;">Backgrou</span><span style="font-family:Verdana;">nd/Aim: Kuji amber is an interesting natural source for drug discovery</span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">because a new anti-allergic compound, named kujigamberol and several new compounds have been isolatated from it. It was important to evaluate the yield, biological activities and constituents of each methanol extract</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">of Kuji, Iwaki, Choshi, Mizunami and Ube ambers in Japan in order to establish if additional new compounds could be identified in these ambers. Materia</span></span></span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;">ls and </span><span style="font-family:Verdana;">Method: Biological activities of each extract were evaluated using growth-restoring </span><span style="font-family:Verdana;">activity of the mutant yeast strain involving Ca</span><sup><span style="font-family:Verdana;">2+</span></sup><span style="font-family:Verdana;">-signal transduction and inhibi</span><span style="font-family:Verdana;">tion activity of degranulation in rat basophilic leukemia (RBL)-2H3 cells. Constituents</span><span style="font-family:Verdana;"> of each extract were analyzed by high performance liquid chromatography (HPLC). Results: All ambers except Ube amber have growth-restoring activity against the mutant yeast. Both Kuji and Iwaki ambers inhibited the degranulation of RBL-2H3 cells induced by the calcium ionophore A23187 in a dose dependent manner. The main biologically active compound in Kuji amber, kujigamberol, was also isolated from Iwaki amber and analyzed by mass spectrometry (MS) and nuclear magnetic resonance (NMR). Conclusion: Kuji and Iwaki ambers appeared to have the same origin. Choshi, Mizunami, and Ube ambers are valuable sources for biologically active compounds which are different from those of Kuji amber.</span></span></span></span>
