In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH valu...In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH value at 6.0, the terbiumenrofloxacin complex had a characteristic fluorescence peak of Tb3＋ at 545 nm （λex= 328 nm）, which could be used for the determination of enrofloxacin. A simple, rapid and sensitive fluorescence method for the determination of enrofloxacin was thus established, and an optimal reaction condition was selected. Under the optimal reaction condition, there was a good linear relation （r2=0.992 3） between concentration of enrofloxacin in the range of 1.0×10^-1.0×10^-6 g/ml and its fluorescence intensity at 545 nm, with a detection limit of 1.3×10^-9 g/ml; the recovery for enrofloxacin in tablets was 97.7% with a variation coefficient of 1.4%; and for enrofloxacin in fish tissues, the recovery was 79.0%-94.5% with a variation coefficient of 2.0%-7.8%.展开更多
Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA ge...Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water,sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.Results In the detection of clinical samples,FQ-PCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water,sea food and winter-endurance specimen,FQ-PCR has superiority to regular ways.Conclusion The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive,and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.展开更多
使用P-E LS 50荧光光谱仪,在盐酸介质中,用同一激发波长(214nm)激发,分别测定 Pr(Ⅲ)(283urn)和Ce(Ⅲ)(356nm)发射峰强度.线性范围Pr_6O_11为0.20～15μg·ml^(-1);CeO_2为0.01～0.25μg·ml^(-1).检出限Pr_6O_(11)为0.008μg&#...使用P-E LS 50荧光光谱仪,在盐酸介质中,用同一激发波长(214nm)激发,分别测定 Pr(Ⅲ)(283urn)和Ce(Ⅲ)(356nm)发射峰强度.线性范围Pr_6O_11为0.20～15μg·ml^(-1);CeO_2为0.01～0.25μg·ml^(-1).检出限Pr_6O_(11)为0.008μg·ml^(-1);CeO_2为0.0011μg·ml^(-1).讨论了盐酸浓度、仪器带通、荧光稳定性及基体氧化钕对错铈测定的影响.结果表明,仪器带通选择B_(ex)=4.0nm,B_(em)=20.0nm及盐酸浓度为8+92时,Pr(Ⅲ)和Ce(Ⅲ)有较稳定的荧光强度,且基体氧化钛对镨铈测定无影响.用此法直接测定高纯氧化钕样品中痕量镨铈,结果令人满意.展开更多
基金Supported by Scientific Research Fund of Hanshan Normal University(511043)~~
文摘In order to establish a new method for the determination of enrofloxacin, on the basis of the fluorescence characteristic of terbium-enrofloxacin complex, it was found that in a HAc-NaAc buffer solution with a pH value at 6.0, the terbiumenrofloxacin complex had a characteristic fluorescence peak of Tb3＋ at 545 nm （λex= 328 nm）, which could be used for the determination of enrofloxacin. A simple, rapid and sensitive fluorescence method for the determination of enrofloxacin was thus established, and an optimal reaction condition was selected. Under the optimal reaction condition, there was a good linear relation （r2=0.992 3） between concentration of enrofloxacin in the range of 1.0×10^-1.0×10^-6 g/ml and its fluorescence intensity at 545 nm, with a detection limit of 1.3×10^-9 g/ml; the recovery for enrofloxacin in tablets was 97.7% with a variation coefficient of 1.4%; and for enrofloxacin in fish tissues, the recovery was 79.0%-94.5% with a variation coefficient of 2.0%-7.8%.
文摘Objective To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.Methods A diagnostic kit(FQ-PCR) was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water,sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.Results In the detection of clinical samples,FQ-PCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water,sea food and winter-endurance specimen,FQ-PCR has superiority to regular ways.Conclusion The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive,and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.
文摘使用P-E LS 50荧光光谱仪,在盐酸介质中,用同一激发波长(214nm)激发,分别测定 Pr(Ⅲ)(283urn)和Ce(Ⅲ)(356nm)发射峰强度.线性范围Pr_6O_11为0.20～15μg·ml^(-1);CeO_2为0.01～0.25μg·ml^(-1).检出限Pr_6O_(11)为0.008μg·ml^(-1);CeO_2为0.0011μg·ml^(-1).讨论了盐酸浓度、仪器带通、荧光稳定性及基体氧化钕对错铈测定的影响.结果表明,仪器带通选择B_(ex)=4.0nm,B_(em)=20.0nm及盐酸浓度为8+92时,Pr(Ⅲ)和Ce(Ⅲ)有较稳定的荧光强度,且基体氧化钛对镨铈测定无影响.用此法直接测定高纯氧化钕样品中痕量镨铈,结果令人满意.
