Epistasis is generally defined as the interaction between two or more genes or their mRNA or protein products to influence a single trait. Experimental evidence suggested that epistasis could be important in the deter...Epistasis is generally defined as the interaction between two or more genes or their mRNA or protein products to influence a single trait. Experimental evidence suggested that epistasis could be important in the determination of the genetic architecture of complex traits in domestic animals. Acetyl-coenzyme A carboxylase alpha （ACACA） and fatty acid binding protein 2 （FABP2） are both key factors of lipogenesis and transport. They may play a crucial role in the weight variability of abdominal adipose tissue in the growing chicken. In this study, the polymorphisms of c.2292G〉A in A CA CA and c.-561A〉C in FABP2 were detected among individuals from two broiler lines which were divergently selected for abdominal fat content. Epistasis between the two SNPs on abdominal fat weight （AFW） and ab- dominal fat percentage （AFP） was analyzed. The additive x additive epistatic components between these two SNPs were found significant or suggestively significant on both AFW and AFP in lean lines of the 9th and 10th generation; whereas, it was not significantly associated with either AFW or AFP in fat lines. At the same time, there were not any other significant epistatic components found in both generations or in both lines. Significant epistatic effects between these two SNPs found only in the lean lines could partly be due to the fact that the abdominal fat traits in these two experimental lines have been greatly modified by strong artificial selection. The results suggested that the epistasis mode may be different between the lean and fat chicken lines. Our results could be helpful in further understanding the genetic interaction between candidate genes contributing to phenotypic variation of abdominal fat content in broilers.展开更多
Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned f...Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned from Spodoptera litura.RT-PCR and Western blot analysis indicated that RNA and protein levels of S/FABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage.The expression of S/FABP2 protein was induced by starvation treatment.In vitro binding assay revealed that the recombinant S/FABP2 had high mffinities of binding Iong-chain fatty acids,such as palmitic acid,arachidonate and oleic acid.The results suggest that S/FABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis.This work provides experimental clues for understanding the potential function of S/FABP2 in fatty acid metabolism in S.litura.展开更多
Objective. To ascertain the relationship between the Ala54Thr variation of FABP2 gene and general as well as regional adipose tissue depots. Subjects. 165 subjects, in which 86 were sub...Objective. To ascertain the relationship between the Ala54Thr variation of FABP2 gene and general as well as regional adipose tissue depots. Subjects. 165 subjects, in which 86 were subjects with normal glucose tolerance (NGT) [age 54 45±9 80, male/female 1 05,body mass index (BMI)26 48±4 01] and 79 were subjects with non insulin dependent diabetes mellitus (NIDDM)(age 55 86±10 00,male/female 1 08,BMI 26 75±3 30). Design and measurements. An association study of FABP2 Ala54Thr variation detected by PCR/HhaI digestion with general and regional adipose tissue depots determined by BMI and magnetic resonance imaging [abdominal subcutaneous and visceral adipose tissue area (SA and VA) and femoral subcutaneous adipose tissue area (FA)]. Results. The geneotype and allele frequencies of FABP2 Ala54Thr variation in Chinese were quite close to the frequencies in American Caucasians and Pima Indians reported in the literature. Significant difference in genotype frequency distribution was observed between FA subgroups comparisons (FA≥75cm 2 versus FA<75cm 2 )in NIDDM subjects (X 2 =11 460,P=0 003),with significantly increased in Thr54 carrier[Thr54(+)]genotype frequency and Thr54 allele frequency in NIDDM subject with FA<75cm 2 (odd ratio for genotype was 4 62,X 2 =10 112,P=0 001;and for allele=2 36,X 2 =5 379,P=0 020).The FA in NIDDM Thr54(+)subgroup was significantly lower than that in subjects with NIDDM Thr54( )sugroup(61 19±21 51cm 2 versus 75 36±31 70cm 2 ,P=0 021). Stepwise regression analysis revealed that FABP2 Thr54 genotype variation was an independent factor contributing to the variation of FA in NIDDM(P=0 003). Conclusion. FABP2 is associated with regional adipose tissue depot.The decreased femoral subcutaneous adipose tissue depot in NIDDM subjects is related to FABP2 Thr54 variant.展开更多
基金supported by the National High-tech R&D Program (863 Program) (No. 2006AA10A120)the National Basic Research Program of China (973 Program) (No. 2006CB102105)the Earmarked Fund for Modern Agro-industry Technology Research system (No. nycytx-42)
文摘Epistasis is generally defined as the interaction between two or more genes or their mRNA or protein products to influence a single trait. Experimental evidence suggested that epistasis could be important in the determination of the genetic architecture of complex traits in domestic animals. Acetyl-coenzyme A carboxylase alpha （ACACA） and fatty acid binding protein 2 （FABP2） are both key factors of lipogenesis and transport. They may play a crucial role in the weight variability of abdominal adipose tissue in the growing chicken. In this study, the polymorphisms of c.2292G〉A in A CA CA and c.-561A〉C in FABP2 were detected among individuals from two broiler lines which were divergently selected for abdominal fat content. Epistasis between the two SNPs on abdominal fat weight （AFW） and ab- dominal fat percentage （AFP） was analyzed. The additive x additive epistatic components between these two SNPs were found significant or suggestively significant on both AFW and AFP in lean lines of the 9th and 10th generation; whereas, it was not significantly associated with either AFW or AFP in fat lines. At the same time, there were not any other significant epistatic components found in both generations or in both lines. Significant epistatic effects between these two SNPs found only in the lean lines could partly be due to the fact that the abdominal fat traits in these two experimental lines have been greatly modified by strong artificial selection. The results suggested that the epistasis mode may be different between the lean and fat chicken lines. Our results could be helpful in further understanding the genetic interaction between candidate genes contributing to phenotypic variation of abdominal fat content in broilers.
基金The study was supported by the National Natural Science Foundation of China(31330071 and 31772519)and the Natural Science Foundation of Guangdong Province,China(2017A030313210).
文摘Fatty acid-bindi ng proteins(FABPs)are a family of lipid chaper on es,which con tribute to systemic metabolic regulati on through diverse lipid signalings.In this study,a midgut-specific FABP gene(Slfabp2)was cloned from Spodoptera litura.RT-PCR and Western blot analysis indicated that RNA and protein levels of S/FABP2 gradually increased and reached a peak at the prepupal stage and maintained a high level during the pupal stage.The expression of S/FABP2 protein was induced by starvation treatment.In vitro binding assay revealed that the recombinant S/FABP2 had high mffinities of binding Iong-chain fatty acids,such as palmitic acid,arachidonate and oleic acid.The results suggest that S/FABP2 may have a unique function that transports intracellular fatty acids and can regulate the metabolism of lipids in metamorphosis.This work provides experimental clues for understanding the potential function of S/FABP2 in fatty acid metabolism in S.litura.
文摘Objective. To ascertain the relationship between the Ala54Thr variation of FABP2 gene and general as well as regional adipose tissue depots. Subjects. 165 subjects, in which 86 were subjects with normal glucose tolerance (NGT) [age 54 45±9 80, male/female 1 05,body mass index (BMI)26 48±4 01] and 79 were subjects with non insulin dependent diabetes mellitus (NIDDM)(age 55 86±10 00,male/female 1 08,BMI 26 75±3 30). Design and measurements. An association study of FABP2 Ala54Thr variation detected by PCR/HhaI digestion with general and regional adipose tissue depots determined by BMI and magnetic resonance imaging [abdominal subcutaneous and visceral adipose tissue area (SA and VA) and femoral subcutaneous adipose tissue area (FA)]. Results. The geneotype and allele frequencies of FABP2 Ala54Thr variation in Chinese were quite close to the frequencies in American Caucasians and Pima Indians reported in the literature. Significant difference in genotype frequency distribution was observed between FA subgroups comparisons (FA≥75cm 2 versus FA<75cm 2 )in NIDDM subjects (X 2 =11 460,P=0 003),with significantly increased in Thr54 carrier[Thr54(+)]genotype frequency and Thr54 allele frequency in NIDDM subject with FA<75cm 2 (odd ratio for genotype was 4 62,X 2 =10 112,P=0 001;and for allele=2 36,X 2 =5 379,P=0 020).The FA in NIDDM Thr54(+)subgroup was significantly lower than that in subjects with NIDDM Thr54( )sugroup(61 19±21 51cm 2 versus 75 36±31 70cm 2 ,P=0 021). Stepwise regression analysis revealed that FABP2 Thr54 genotype variation was an independent factor contributing to the variation of FA in NIDDM(P=0 003). Conclusion. FABP2 is associated with regional adipose tissue depot.The decreased femoral subcutaneous adipose tissue depot in NIDDM subjects is related to FABP2 Thr54 variant.
