Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,...Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,promoting blood and detumescence.Solanum Nigrum Linn fruit contains a variety of antioxidant enzymes,can remove the body produced by aerobic metabolism harmful substances.In this paper,a model of alcohol-induced liver injury in C57BL/6 mice was established to evaluate the protective effect of Solanum Nigrum Linn green fruit(SNGF)ethanolic extract on alcohol-induced liver injury.H&E staining and oil red O(ORO)staining showed that hepatic lobules were clearly demarcated,vacuoles were significantly reduced and lipid droplets were reduced in SNGF ethanolic extract treatment group.Serum levels of TC,TG,LDH,TBA,AKP,ALT and AST were decreased in the SNGF ethanolic extract treatment group,and SNGF ethanolic extract could clear reactive oxygen species(ROS)in time.MDA content was signifi cantly decreased after SNGF ethanolic extract treatment,while superoxide dismutase(SOD)and GSH-Px contents were increased after SNGF ethanolic extract treatment.These results suggest that SNGF ethanolic extract has a protective effect on alcohol-induced liver injury.展开更多
Background:Artemisia vulgaris,a medicinal aromatic plant,is widely used as a food item,tonic pharmaceutical,and cosmetic industry additive owing to its antibacterial,antihypertensive,hepatoprotective,antioxidant,and a...Background:Artemisia vulgaris,a medicinal aromatic plant,is widely used as a food item,tonic pharmaceutical,and cosmetic industry additive owing to its antibacterial,antihypertensive,hepatoprotective,antioxidant,and antispasmodic properties.But the effect of different geographic locations on the chemical composition and bioactivities of its extracts is unclear.Methods:Biological activities of essential oils and ethanol extracts of three varieties of Artemisia vulgaris leaves,which are grown in Shanxi province China,were studied.Results:Gas chromatography-mass spectrometry analysis revealed that the main components of essential oils were terpenes and ketones.Essential oils and ethanol extract of Artemisia vulgaris leaves possessed good antioxidant activities,and their half maximal inhibitory concentrations determined using 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)assays were 57.0 and 22.9μg/mL,respectively.The essential oils also exhibited remarkable antibacterial activity against three foodborne pathogenic bacterial strains.The ethanol extract presented a high anticancer activity against the MGC-803 human gastric cancer cell line.Conclusion:These biological activities were well correlated with the composition of the extract and EOs,which in turn is affected by the genetic composition of Artemisia vulgaris and geographic location and diverse climatic condition under which it is grown.These findings demonstrate the remarkable potential of Artemisia vulgaris as a valuable source of antioxidant,antibacterial,and anticancer agents.展开更多
Blackberry (Rubus fructicosus) is one of the fruit with the highest concentration of anthocyanins;however, its use is limited for making jams, jellies and liqueur, and recently, fruit concentrates in combination with ...Blackberry (Rubus fructicosus) is one of the fruit with the highest concentration of anthocyanins;however, its use is limited for making jams, jellies and liqueur, and recently, fruit concentrates in combination with pomegranate, blueberry and grape. One of the main problems with these pigments is their poor stability in solution, mainly in beverages as liqueur and juices, which depends on factors such as chemical structure, pH, temperature, light, water activity, and presence of oxygen. The effect of light on total monomeric anthocyanins content as well as the degradation rates and browning of two blackberry ethanolic extracts is to establish the conditions for storage of liqueur without adding artificial food coloring. The initial content of anthocyanins on extract without storage was 106 mg?l-1. The study assessed the light irradiation effect on the anthocyanins of blackberry ethanolic extract. The anthocyanins degradation followed the second order reaction kinetics with respect to illuminance of the light source. The t1/2 value at high illuminance (3968.30 lx) was 28.20 hours.展开更多
<strong>Objective: </strong>The present was initiated to study the antibacterial properties of the <i>Crateva adansonii</i> DC extract on germs commonly identified in skin and digestive infecti...<strong>Objective: </strong>The present was initiated to study the antibacterial properties of the <i>Crateva adansonii</i> DC extract on germs commonly identified in skin and digestive infections in Benin as well as the reversion of resistance to these aforementioned germs. <strong>Method: </strong>The bacteria’s sensitivity test to extracts was carried out by the microdilution method in liquid medium as well as the MIC and the reversion of bacterial resistance. For the determination of the MBC, this technique is used coupled with spreading on agar medium. <strong>Results: </strong>The results show an antibacterial activity of the extract with MICs between 0.625 - 5 mg/ml. The CMB of <i>Enterococcus faecalis</i> ATCC 29212 is 2.5 mg/ml while that of Methicillin-resistant <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> CIP 8039 is 5 mg/ml. The reversion of bacterial resistance has shown a synergy of action between our extract and conventional antibiotics.展开更多
Plants are sources of medicinal compounds,and they play a crucial role in human health maintenance.Abrus precatorius is one of the important medicinal plants that have been alleged for their medicinal properties.This ...Plants are sources of medicinal compounds,and they play a crucial role in human health maintenance.Abrus precatorius is one of the important medicinal plants that have been alleged for their medicinal properties.This research unraveled the pharmacological effect of ethanolic extract of Abrus precatorius on lipid peroxidation,liver parameters,and Na^(+)/K^(+)-ATPase activity in HgCl_(2) treated wistar rats.Twenty-four(24)albino wistar rats weighing between 150-200 g were distributed into four groups of 6 animals each.Group A(control)received normal saline(0.9% NaCl),group B received 400 mg/kg of the extract only,group C received 4 mg/kg HgCl_(2) only,and group D received 400 mg/kg of extract+4 mg/kg of HgCl_(2).The treatment lasted for two weeks,and the animals were sacrificed on the 15th day.The blood,brains and livers were collected and used for assay of lipid peroxidation,liver function,and sodium pump activity.The results of liver function test revealed an elevated(P<0.05)level of serum aspartate transaminase,alanine transaminase,alkaline phosphatase,and total bilirubin in the group that received HgCl_(2) only(group C)when compared with the normal control(group A)that received normal saline only.However,the administration of extract in group D led to a marked(P<0.05)reduction in the activities of these enzymes and the level of total bilirubin when compared to the negative control(group C).On the contrary,HgCl_(2) caused a significant(P<0.05)reduction in serum total protein and albumin levels,but the extract reversed the effect of HgCl_(2) by elevating their concentrations.Nonetheless,the effect elicited by this extract is comparable to group A which received normal saline.Moreso,the result of lipid peroxidation revealed that HgCl_(2) treatment caused a marked(P<0.05)increase in the formation of lipid peroxidation adducts in both liver and brain homogenates in group C.On the contrary,administration of Abrus precatorius extract profoundly(P<0.05)inhibited HgCl_(2)-induced lipid peroxidation in group D.In addition,HgCl_(2) inhibited the activity of cerebral Na^(+)/K^(+)-ATPase,but the extract restored normalcy by increasing the activity of the enzyme in group D.Consequently,the results obtained justify the traditional use of Abrus precatorius and suggest that Abrus precatorius leaves may be used for management of liver diseases,oxidative stress-linked diseases and some neurodegenerative ailments.展开更多
Objective:To investigate the effect of the ethanolic extract of Rosa laevigata Michx.fruit on rats with mesangial proliferative glomerulonephritis based on the NLRP3 inflammasome pathway.Methods:Thirty Wistar rats wer...Objective:To investigate the effect of the ethanolic extract of Rosa laevigata Michx.fruit on rats with mesangial proliferative glomerulonephritis based on the NLRP3 inflammasome pathway.Methods:Thirty Wistar rats were divided into three groups,a blank control group,a diabetic nephropathy(DN)model group,and an ethanolic extract intervention group,according to the random number table method,with 10 rats in each group.One day before the experiment,basic feeding was initiated for all the rats;the changes in activity and weight of each group of rats were observed and recorded after 7 d,and a rat model of renal function injury was established after 1 d.Results:Compared with the control group,the model group had significantly higher kidney/body ratio,24 h urine protein,serum creatinine(SCr),blood urea nitrogen(BUN),glomerular mesangial cell(GMC)count,and extracellular matrix(ECM)positive area ratio(P<0.05);the same indicators were significantly lower in the intervention group than in the model group(P<0.05).The NLRP3 inflammasome pathway in renal intrinsic cells was activated in the intervention group.The overactivation of NLRP3 inflammasome is known to promote interleukin(IL)-1βrelease,which was inhibited in the intervention group.Conclusion:The ethanolic extract of Rosa laevigata Michx.fruit has a protective effect on renal intrinsic cells and may be related to NLRP3 inflammasome pathway,suggesting that the fruit of Rosa laevigata Michx.has a potential role in protecting renal intrinsic cells from inflammatory damage.NLRP3 inflammasomes are involved in the development of various chronic inflammatory diseases,such as acute and chronic glomerulonephritis and renal fibrosis.展开更多
OBJECTIVE： Use of cisplatin, a conventional anticancer drug, is restricted because it generates strong hepatotoxicity by accumulating in liver. Therefore its anticancer potential can only be fully exploited if its ow...OBJECTIVE： Use of cisplatin, a conventional anticancer drug, is restricted because it generates strong hepatotoxicity by accumulating in liver. Therefore its anticancer potential can only be fully exploited if its own toxicity is considerably reduced. Towards this goal, ethanolic extract of the plant, Boldo （Peumus boldus）, known for its antihepatotoxic effects, was used simultaneously with cisplatin, to test its ability to reduce cisplatin＇s cytotoxicity without affecting its anticancer potential. METHODS： The cytotoxicity of Boldo extract （BE） and cisplatin, administered alone and in combination, was determined in three cancer cell lines （A549, HeLa, and HepG2） and in normal liver cells （WRL-68）. Drug-DNA interaction, DNA damage, cell cycle, apoptosis, reactive oxygen species （ROS） and mitochondrial membrane potential （MMP, Aψ） were also studied. Hepatotoxicity and antioxidant activity levels were determined by alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and glutathione assays in mice. The cytotoxicity of related proteins was tested by Western blotting. RESULTS： Co-administration of BE and cisplatin increased viability of normal cells, but had no effect on the viability of cancer cells. Boldo protected liver from damage and normalized different antioxidant enzyme levels in vivo and also reduced ROS and re-polarized MMP in vitro. Bax and cytochrome c translocation was reduced with caspase 3 down-regulation. Further, a drug- DNA interaction study revealed that BE reduced cisplatin＇s DNA-binding capacity, resulting in a reduction in DNA damage. CONCLUSION： Results indicated that a low dose of BE could be used beneficially in combination with cisplatin to reduce its toxicity without hampering cisplatin＇s anticancer effect. These findings siqnify a potential future use of BE in cancer therapy.展开更多
Objective： To identify the free radical scavenging activity of ethanolic extract of Evolvulus alsinoides. Methods： The free radical scavenging activity was evaluated by in vitro methods like reducing power assay, to...Objective： To identify the free radical scavenging activity of ethanolic extract of Evolvulus alsinoides. Methods： The free radical scavenging activity was evaluated by in vitro methods like reducing power assay, total antioxidant activity, 2,2-diphenyl-l-picrylhydrazyl （DPPH） reduction, superoxide radical scavenging activity, 2,2＇-azinobis-（3-ethylbenzothiazoline-6-sulfonic acid） （ABTS＊） scavenging activity, hydroxyl radical scavenging assay, and nitric oxide radical scavenging assay, which were studied by using ascorbic acid as standard. Results： The extract showed significant activities in all antioxidant assays compared with the reference antioxidant ascorbic acid. The total antioxidant activity as well as the reducing power was also found to increase in a dose-dependent manner. Conclusion： Evolvulus alsinoides may act as a chemopreventive agent, providing antioxidant properties and offering effective protection from free radicals.展开更多
Objective:To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams’semen extender for obtaining the best-cryopreserved semen quality.Methods:Tris-based semen extender was supple...Objective:To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams’semen extender for obtaining the best-cryopreserved semen quality.Methods:Tris-based semen extender was supplemented with 0.0,0.40,0.48,and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months(n=80).Pooled(n=16)post-thaw semen characteristics were determined.Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde(MDA),nitric oxide(NO),glutathione peroxidase(GPx),superoxide dismutase(SOD),ascorbic acid,zinc,copper,total cholesterol,low-density lipoproteins(LDL),lactate dehydrogenase(LDH),and alkaline phosphatase(ALP).Results:The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility(P<0.001),sperm progressive motility(P<0.001),live sperm(P<0.001),sperm plasma membrane integrity(P<0.001),acrosome integrity(P<0.001),SOD(P<0.05),zinc(P<0.001),total cholesterol(P<0.001),and LDL(P<0.001)with the lowest percentage of abnormal sperm morphology(P<0.001),the lowest lipid peroxidation(MDA,P<0.01),ascorbic acid(P>0.05),and LDH(P>0.05).Conclusions:Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.展开更多
Objective:To compare the antibacterial activity and antiseptic effect of ethanol extract of Peganum harmala L.(P.harmala)seeds and Betadine on eight reference strains of Streptococcus pneumoniae,Salmonella typhimurium...Objective:To compare the antibacterial activity and antiseptic effect of ethanol extract of Peganum harmala L.(P.harmala)seeds and Betadine on eight reference strains of Streptococcus pneumoniae,Salmonella typhimurium,Escherichia coli,Corynebacterium pseudotuberculosis,Klebsiella pneumoniae,Staphylococcus aureus,Micrococcus luteus which are known to cause different types of skin infections,and Corynebacterium pseudotuberculosis isolated from an abscess on horse's neck.Methods:The antibacterial activity was assessed using a disc diffusion method.The minimum inhibitory concentration was tested by serial dilution method and the percentage of bacterial growth inhibition by absorbance microplate reader device.The minimum bactericidal concentration was then recorded.Clinical outcomes were obtained through washed up healing time of longitudinal and surface skin on the back of 16 rats with concentrations of 10,50,100,150 and 500 mg/mL of P.harmala extract.Results:The results of in vitro experiments showed that the lowest minimum inhibitory concentration(0.68-1.3 mg/mL)and minimum bactericidal concentration(1.3-5 mg/mL)values were observed in the ethanol extract of P.harmala seeds.Also the results of in vivo experiment showed that wound healing in the concentration of 50 mg/mL of this plant extract was better and quicker than Betadine.Conclusions:This study confirmed that ethanol extract of P.harmala has appropriate effect on the microorganisms and the healing of skin wounds in comparison with Betadine.展开更多
Background and objective:In northern China's cold regions,the prevalence of metabolic dysfunction-associated steatotic liver disease(MASLD)exceeds 50%,significantly higher than the national and global rates.MASLD ...Background and objective:In northern China's cold regions,the prevalence of metabolic dysfunction-associated steatotic liver disease(MASLD)exceeds 50%,significantly higher than the national and global rates.MASLD is an important risk factor for cardiovascular and cerebrovascular diseases,including coronary heart disease,stroke,and tumors,with no specific therapeutic drugs currently available.The ethanol extract of cassia seed(CSEE)has shown promise in lowering blood lipids and improving hepatic steatosis,but its mechanism in treating MASLD remains underexplored.This study aims to investigate the therapeutic effects and mechanisms of CSEE.Methods:MASLD models were established in male Wistar rats and golden hamsters using a high fat diet(HFD).CSEE(10,50,250 mg/kg)was administered via gavage for six weeks.Serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),as well as liver TC and TG,were measured using biochemical kits.Histopathological changes in the liver were evaluated using Oil Red O staining,Hematoxylin-eosin(H&E)staining,and transmission electron microscopy(TEM).HepG2 cell viability was assessed using the cell counting kit-8(CCK8)and Calcein-AM/PI staining.Network pharmacology was used to analyze drug-disease targets,and western blotting was used to confirm these predictions.Results:CSEE treatment significantly reduced serum levels of TC,TG,LDL-C,ALT,and AST,and improved liver weight,liver index,and hepatic lipid deposition in rats and golden hamsters.In addition,CSEE alleviated free fatty acid(FFA)-induced lipid deposition in HepG2 cells.Molecular biology experiments demonstrated that CSEE increased the protein levels of p-AMPK,p-ACC,PPARα,CPT1A,PI3K P110 and p-AKT,while decreasing the protein levels of SREBP1,FASN,C/EBPα,and PPARγ,thus improving hepatic lipid metabolism and reducing lipid deposition.The beneficial effects of CSEE were reversed by small molecule inhibitors of the signaling pathways in vitro.Conclusion:CSEE improves liver lipid metabolism and reduces lipid droplet deposition in Wistar rats and golden hamsters with MASLD by activating hepatic AMPK,PPARα,and PI3K/AKT signaling pathways.展开更多
[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the co...[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC.The HPLC conditions were as follows;Alltima NH2 （5 μm,4.6 mm × 150 mm） column separation;the mobile phase was acetonitrile-2%H3PO4 （90：10）;the flowing velocity was 1 ml/min;test wavelength was 213 nm,the width of belt was 16 nm;reference wavelength was 300 nm,the width of belt was 80 nm.[Result] By the single factor and orthogonal tests,the optimum conditions were found as follows：ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1：25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%.[Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.展开更多
Objective To investigate the antidiabetic, antioxidant and hypolipidemic efficacy of Cynodon dactylon in diabetic rats. Methods The experimental rats were randomly divided into three groups： Group I： control; Group...Objective To investigate the antidiabetic, antioxidant and hypolipidemic efficacy of Cynodon dactylon in diabetic rats. Methods The experimental rats were randomly divided into three groups： Group I： control; Group II： Alloxan diabetic, untreated; and Group III： Alloxan diabetic treated with ethanolic extract of C. dactylon leaves （450 mg/kg bw）. Experimental diabetes was induced by alloxan in a single dose of 150 mg/kg bw. Results A Significant diminution of fasting blood sugar level was observed and also significant increase in HDL and decrease （P0.05） in cholesterol, triglyceride, LDL and VLDL were observed after 15 days of treatment. The investigation also revealed, the activities of AST, ALT, ALP, AP, LDH, and CPK （P0.05） were decreased in the extract‐supplemented group. The significant decrease in protein content and SOD, CAT, GPx, and GSH （P0.05） activity and increase in LPO in plasma were found to be ameliorated after treatment. Conclusion Our result supports the fact that administration of extract of C. dactylon leave is able to reduce hyperglycemia and hyperlipidemia risk and also reduced the oxidative stress in diabetic rats.展开更多
[Objective]The aim was to study the sedative and hypnotic effects of Tartary Buckwheat ethanol extract and lay a foundation for further expanding the application of Tartary Buckwheat. [Method]The effects of Tartary Bu...[Objective]The aim was to study the sedative and hypnotic effects of Tartary Buckwheat ethanol extract and lay a foundation for further expanding the application of Tartary Buckwheat. [Method]The effects of Tartary Buckwheat ethanol extract on pentobarbital sodium and mice spontaneous action times were recorded. [Result]Ethanol extract of Tartary Buckwheat could prolong mice sleep duration caused by pentobarbital sodium （suprathreshold dosage）,increase mice sleep number caused by pentobarbital sodium （subthreshold dosage）,and obviously inhibit mice spontaneous action times. [Conclusion]Ethanol extract of Tartary Buckwheat has a function of sedation.展开更多
[Objective] The aim was to compare the molluscicide activity of ethanol extract of Sapium sebiferum,Pterocarya stenoptera and Cryptomeria fortunei.[Method] The molluscicide activity of ethanol extract of S.sebiferum,P...[Objective] The aim was to compare the molluscicide activity of ethanol extract of Sapium sebiferum,Pterocarya stenoptera and Cryptomeria fortunei.[Method] The molluscicide activity of ethanol extract of S.sebiferum,P.stenoptera and C.fortunei was determined through poison test of Oncomelania hupensis in laboratory.[Result] S.sebiferum,P.stenoptera and C.fortunei had certain molluscicide effect,and S.sebiferum and C.fortunei had better effect with little difference than P.stenoptera;ethanol could extract the effective molluscicide component from plant more completely,and its molluscicide effect was better than that of plant infusion.[Conclusion] Our study could provide theory reference for the plant selection of controlling O.hupensis ecologically.展开更多
Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride(CCl_4) induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperi...Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride(CCl_4) induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperitoneal injection of CCl_4,1 mL/kg body weight for every 72 h.The ethanolic extract of Premna corymbosa leaves were administrated at doses of 200 & 400 mL/kg body weight, p.o.,daily for 14 days.The hepatotoxicity and its prevention was assessed by serum markers like serum alkaline phosphatase(SALP),serum triglycerides(STG),serum total protein(STP), serum cholesterol(SC),and liver wet weight and histopathological studies of the liver.Results:In treatment with the ethanolic extract,the toxic effect of CCl_4 was controlled significantly(P<0.01) by restoration of the levels of biochemical parameters as compared to normal and standard drug silymarin treated groups.The liver weight was reduced by the ethanolic extract treated groups. The histopathology of the liver sections evidenced the hepatoprotective activity.Conclusion:The ethanolic extract of the leaves of Premna corymbosa possess significant acute hepatoprotective activity.Premna corymbosa can be recommended for the liver disorders.展开更多
Ethanolic extracts of five plant material from Eucalyptus gluaca, Melia azadrach, Mentha arvensis, Olea europaea leaves and periearp of Punica granatum were investigated for insecticidal activity against the confused ...Ethanolic extracts of five plant material from Eucalyptus gluaca, Melia azadrach, Mentha arvensis, Olea europaea leaves and periearp of Punica granatum were investigated for insecticidal activity against the confused flour beetle Tribolium confusum DuVal in the laboratory. Different concentrations 2.5%, 5%, 7.5%, 10% using filter paper test were implemented in the experiment. Mortality was assessed after 24, 48, 72 hrs of treatment and LDs0 were calculated at respective time intervals. Data analysis was made by using factorial Complete Randomized Design （CRD）. The results revealed that all of the tested materials with some variations had lethal effects against the pest as compared with the untreated check. The time 24 h showed higher insecticidal effect with the tested concentrations for the larvae and adult of Tribolium confusum. Strong toxicity effect was observed at concentration 5% of M.. arvensis for adult followed by E. gluaca, O. europaea, M.. azedrach and P. granatum. For larval stage the extract of O. europaea and E. gluaca with concentration 7.5%, 1%, respectively caused high mortality followed by M. arvensis, M. azedrach and P. granatum. The LDs0 values for adults and larvae were calculated to be 1.33 and 1.42 with M.. arvensis and O. europaea, respectively.展开更多
The purpose of the study was to evaluate the efficiency of the microwave (MW) extraction method by comparing it with a conventional method through evaluation of antimicrobial and synergism/antagonism activity of each ...The purpose of the study was to evaluate the efficiency of the microwave (MW) extraction method by comparing it with a conventional method through evaluation of antimicrobial and synergism/antagonism activity of each aquatic and ethanolic extracts samples, which were extracted from the dried plants (<em>Ficus sycomorus</em> leaves, <em>Lawsonia inermis</em> leaves and <em>Glycerrhiza glabra</em> Linn.). Nine samples of each plant for both solvents were irradiated with MW at several power outputs (180 w, 360 w, and 540 w) in several interval times (1, 2 and 3 minutes). The antibacterial activities of extracts and the synergistic effect between plants and antibiotics were evaluated using disk diffusion method against clinical isolated <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. The result revealed that the inhibition zone for more than 50% using aquatic and ethanolic samples results (extracted in two minutes and MW power 180 w) had shown the optimum extract and better antibiotic activity for each plant. Also, the results of ethanolic extracts used against selected microorganisms showed antimicrobial and synergistic effect with most antibiotics better than aquatic extracts. Our results indicate the possibility of using MW apparatus as an extractor to obtain bioactive compounds from plants and thus used in the treatment of bacterial infections, and some results of this study were encouraging. However, the antagonistic reactions of some extracts with some antibiotics and their use in combination should be further studied for<em> in vitro</em> activities. It is clearly a need to be furthermore evaluated, to identify the effective components, the mode of action and the possible toxic effect <em>in-vivo</em> of these ingredients.展开更多
Lagenaria siceraria （Molina） Standl., known as bottle gourd is widely available throughout Vietnam as an edible vegetable. Bottle gourd is a commonly fruit but there are little reports about its biological activitie...Lagenaria siceraria （Molina） Standl., known as bottle gourd is widely available throughout Vietnam as an edible vegetable. Bottle gourd is a commonly fruit but there are little reports about its biological activities. The aim of the present study was to evaluate antioxidant activity and anti-hyperglycemic potential of ethanolic extract of bottle gourd. The ethanolic extract was tested for its antioxidant activity on chelating power, the ferric reducing/antioxidant power assay （FRAP） and the 1, 1-diphenyl-2-picryl-hydrazyl （DPPH） free radical scavenging assay. The results showed that chelating activity was 80.02%, FRAP value was 92.84 μmol Fe^2＋/g and active radical scavenging which showed an IC50 was 49.50 μg/ml. The five different doses of ethanolic extract were treated by oral tolerance to mice body before oral glucose tolerance and blood glucose level was measured using glucose oxidase method. The result indicated that inhibition percentage of ethanolic extract of bottle gourd at the dose of 400 mg/kg in glucose-induced hyperglycemic mice was effectively similar to standard drug glibenclamide. The results obtained indicate that ethanolic extract from bottle gourd is the potential antioxidant and anti-hyperglycemic agent.展开更多
Plants of Baccharis （Asteraceae） genus are commonly known in Argentina as ＂carqueja＂. The antimicrobial activity and minimal inhibitory concentration of B. articulata, B. trimera and B. crispa aqueous and ethanoli...Plants of Baccharis （Asteraceae） genus are commonly known in Argentina as ＂carqueja＂. The antimicrobial activity and minimal inhibitory concentration of B. articulata, B. trimera and B. crispa aqueous and ethanolic extracts were evaluated by using the micro-well dilution method. Previously, the components of extracts were analyzed by spectroscopial means. Gram-positive bacteria were more sensitive to Baccharis species extracts than Gram-negative bacteria. Out of 3 plant species, B. trimera showed significant antibacterial activity and aqueous and ethanolic extracts were active against Staphylococcus aureus （MIC = 2,500 μg/mL and 1,250 μg/mL, respectively） and Listeria monocytogenes （MIC = 625 μg/mL and 625 μg/mL, respectively）. All ethanolic extracts inhibited the growth of the selected Gram-positive （MIC values ranged between 625 μg/mL and 1,250 μg/mL）. Therefore, all Gram-negative bacteria were resistant to the ethanolic and aqueous extracts tested. One flavone, genkawanin, was identified from the three ethanolic extracts as the responsible of antibacterial activity. Two terpenes, hawtriwaic acid and bacrispine, were identified from ethanolic extract of B. crispa and B. trimera as the responsibles of antibacterial activity. These preliminary studies corroborated the antimicrobial activity of the selected plants used in folklore medicine. Therefore, they could be potential sources of new antimicrobial agents used in treatment of infectious diseases.展开更多
文摘Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,promoting blood and detumescence.Solanum Nigrum Linn fruit contains a variety of antioxidant enzymes,can remove the body produced by aerobic metabolism harmful substances.In this paper,a model of alcohol-induced liver injury in C57BL/6 mice was established to evaluate the protective effect of Solanum Nigrum Linn green fruit(SNGF)ethanolic extract on alcohol-induced liver injury.H&E staining and oil red O(ORO)staining showed that hepatic lobules were clearly demarcated,vacuoles were significantly reduced and lipid droplets were reduced in SNGF ethanolic extract treatment group.Serum levels of TC,TG,LDH,TBA,AKP,ALT and AST were decreased in the SNGF ethanolic extract treatment group,and SNGF ethanolic extract could clear reactive oxygen species(ROS)in time.MDA content was signifi cantly decreased after SNGF ethanolic extract treatment,while superoxide dismutase(SOD)and GSH-Px contents were increased after SNGF ethanolic extract treatment.These results suggest that SNGF ethanolic extract has a protective effect on alcohol-induced liver injury.
基金This work was financially supported by the National Natural Science Foundation of China(Grant No.32001817)the Science and Technology Innovation Plan of Colleges and Universities of Shanxi Province(2020L0298)the College student innovation project of North University of China and the start-up funds for scientific research at North University of China(No.304-1101285714).
文摘Background:Artemisia vulgaris,a medicinal aromatic plant,is widely used as a food item,tonic pharmaceutical,and cosmetic industry additive owing to its antibacterial,antihypertensive,hepatoprotective,antioxidant,and antispasmodic properties.But the effect of different geographic locations on the chemical composition and bioactivities of its extracts is unclear.Methods:Biological activities of essential oils and ethanol extracts of three varieties of Artemisia vulgaris leaves,which are grown in Shanxi province China,were studied.Results:Gas chromatography-mass spectrometry analysis revealed that the main components of essential oils were terpenes and ketones.Essential oils and ethanol extract of Artemisia vulgaris leaves possessed good antioxidant activities,and their half maximal inhibitory concentrations determined using 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)assays were 57.0 and 22.9μg/mL,respectively.The essential oils also exhibited remarkable antibacterial activity against three foodborne pathogenic bacterial strains.The ethanol extract presented a high anticancer activity against the MGC-803 human gastric cancer cell line.Conclusion:These biological activities were well correlated with the composition of the extract and EOs,which in turn is affected by the genetic composition of Artemisia vulgaris and geographic location and diverse climatic condition under which it is grown.These findings demonstrate the remarkable potential of Artemisia vulgaris as a valuable source of antioxidant,antibacterial,and anticancer agents.
文摘Blackberry (Rubus fructicosus) is one of the fruit with the highest concentration of anthocyanins;however, its use is limited for making jams, jellies and liqueur, and recently, fruit concentrates in combination with pomegranate, blueberry and grape. One of the main problems with these pigments is their poor stability in solution, mainly in beverages as liqueur and juices, which depends on factors such as chemical structure, pH, temperature, light, water activity, and presence of oxygen. The effect of light on total monomeric anthocyanins content as well as the degradation rates and browning of two blackberry ethanolic extracts is to establish the conditions for storage of liqueur without adding artificial food coloring. The initial content of anthocyanins on extract without storage was 106 mg?l-1. The study assessed the light irradiation effect on the anthocyanins of blackberry ethanolic extract. The anthocyanins degradation followed the second order reaction kinetics with respect to illuminance of the light source. The t1/2 value at high illuminance (3968.30 lx) was 28.20 hours.
文摘<strong>Objective: </strong>The present was initiated to study the antibacterial properties of the <i>Crateva adansonii</i> DC extract on germs commonly identified in skin and digestive infections in Benin as well as the reversion of resistance to these aforementioned germs. <strong>Method: </strong>The bacteria’s sensitivity test to extracts was carried out by the microdilution method in liquid medium as well as the MIC and the reversion of bacterial resistance. For the determination of the MBC, this technique is used coupled with spreading on agar medium. <strong>Results: </strong>The results show an antibacterial activity of the extract with MICs between 0.625 - 5 mg/ml. The CMB of <i>Enterococcus faecalis</i> ATCC 29212 is 2.5 mg/ml while that of Methicillin-resistant <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> CIP 8039 is 5 mg/ml. The reversion of bacterial resistance has shown a synergy of action between our extract and conventional antibiotics.
文摘Plants are sources of medicinal compounds,and they play a crucial role in human health maintenance.Abrus precatorius is one of the important medicinal plants that have been alleged for their medicinal properties.This research unraveled the pharmacological effect of ethanolic extract of Abrus precatorius on lipid peroxidation,liver parameters,and Na^(+)/K^(+)-ATPase activity in HgCl_(2) treated wistar rats.Twenty-four(24)albino wistar rats weighing between 150-200 g were distributed into four groups of 6 animals each.Group A(control)received normal saline(0.9% NaCl),group B received 400 mg/kg of the extract only,group C received 4 mg/kg HgCl_(2) only,and group D received 400 mg/kg of extract+4 mg/kg of HgCl_(2).The treatment lasted for two weeks,and the animals were sacrificed on the 15th day.The blood,brains and livers were collected and used for assay of lipid peroxidation,liver function,and sodium pump activity.The results of liver function test revealed an elevated(P<0.05)level of serum aspartate transaminase,alanine transaminase,alkaline phosphatase,and total bilirubin in the group that received HgCl_(2) only(group C)when compared with the normal control(group A)that received normal saline only.However,the administration of extract in group D led to a marked(P<0.05)reduction in the activities of these enzymes and the level of total bilirubin when compared to the negative control(group C).On the contrary,HgCl_(2) caused a significant(P<0.05)reduction in serum total protein and albumin levels,but the extract reversed the effect of HgCl_(2) by elevating their concentrations.Nonetheless,the effect elicited by this extract is comparable to group A which received normal saline.Moreso,the result of lipid peroxidation revealed that HgCl_(2) treatment caused a marked(P<0.05)increase in the formation of lipid peroxidation adducts in both liver and brain homogenates in group C.On the contrary,administration of Abrus precatorius extract profoundly(P<0.05)inhibited HgCl_(2)-induced lipid peroxidation in group D.In addition,HgCl_(2) inhibited the activity of cerebral Na^(+)/K^(+)-ATPase,but the extract restored normalcy by increasing the activity of the enzyme in group D.Consequently,the results obtained justify the traditional use of Abrus precatorius and suggest that Abrus precatorius leaves may be used for management of liver diseases,oxidative stress-linked diseases and some neurodegenerative ailments.
基金This work was supported by the Health Commission of Hebei Province under the project Chuanxiong Extract Improves Inflammatory Response in Rats with Pyelonephritis Through IL-6/STAT3 Signaling Pathway(Project Number:20231486).
文摘Objective:To investigate the effect of the ethanolic extract of Rosa laevigata Michx.fruit on rats with mesangial proliferative glomerulonephritis based on the NLRP3 inflammasome pathway.Methods:Thirty Wistar rats were divided into three groups,a blank control group,a diabetic nephropathy(DN)model group,and an ethanolic extract intervention group,according to the random number table method,with 10 rats in each group.One day before the experiment,basic feeding was initiated for all the rats;the changes in activity and weight of each group of rats were observed and recorded after 7 d,and a rat model of renal function injury was established after 1 d.Results:Compared with the control group,the model group had significantly higher kidney/body ratio,24 h urine protein,serum creatinine(SCr),blood urea nitrogen(BUN),glomerular mesangial cell(GMC)count,and extracellular matrix(ECM)positive area ratio(P<0.05);the same indicators were significantly lower in the intervention group than in the model group(P<0.05).The NLRP3 inflammasome pathway in renal intrinsic cells was activated in the intervention group.The overactivation of NLRP3 inflammasome is known to promote interleukin(IL)-1βrelease,which was inhibited in the intervention group.Conclusion:The ethanolic extract of Rosa laevigata Michx.fruit has a protective effect on renal intrinsic cells and may be related to NLRP3 inflammasome pathway,suggesting that the fruit of Rosa laevigata Michx.has a potential role in protecting renal intrinsic cells from inflammatory damage.NLRP3 inflammasomes are involved in the development of various chronic inflammatory diseases,such as acute and chronic glomerulonephritis and renal fibrosis.
基金supported by a grant of University of Grant Commission,New Delhi,India,sanctioned to Jesmin Mondal through Maulana Azad National Fellowship scheme
文摘OBJECTIVE： Use of cisplatin, a conventional anticancer drug, is restricted because it generates strong hepatotoxicity by accumulating in liver. Therefore its anticancer potential can only be fully exploited if its own toxicity is considerably reduced. Towards this goal, ethanolic extract of the plant, Boldo （Peumus boldus）, known for its antihepatotoxic effects, was used simultaneously with cisplatin, to test its ability to reduce cisplatin＇s cytotoxicity without affecting its anticancer potential. METHODS： The cytotoxicity of Boldo extract （BE） and cisplatin, administered alone and in combination, was determined in three cancer cell lines （A549, HeLa, and HepG2） and in normal liver cells （WRL-68）. Drug-DNA interaction, DNA damage, cell cycle, apoptosis, reactive oxygen species （ROS） and mitochondrial membrane potential （MMP, Aψ） were also studied. Hepatotoxicity and antioxidant activity levels were determined by alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and glutathione assays in mice. The cytotoxicity of related proteins was tested by Western blotting. RESULTS： Co-administration of BE and cisplatin increased viability of normal cells, but had no effect on the viability of cancer cells. Boldo protected liver from damage and normalized different antioxidant enzyme levels in vivo and also reduced ROS and re-polarized MMP in vitro. Bax and cytochrome c translocation was reduced with caspase 3 down-regulation. Further, a drug- DNA interaction study revealed that BE reduced cisplatin＇s DNA-binding capacity, resulting in a reduction in DNA damage. CONCLUSION： Results indicated that a low dose of BE could be used beneficially in combination with cisplatin to reduce its toxicity without hampering cisplatin＇s anticancer effect. These findings siqnify a potential future use of BE in cancer therapy.
文摘Objective： To identify the free radical scavenging activity of ethanolic extract of Evolvulus alsinoides. Methods： The free radical scavenging activity was evaluated by in vitro methods like reducing power assay, total antioxidant activity, 2,2-diphenyl-l-picrylhydrazyl （DPPH） reduction, superoxide radical scavenging activity, 2,2＇-azinobis-（3-ethylbenzothiazoline-6-sulfonic acid） （ABTS＊） scavenging activity, hydroxyl radical scavenging assay, and nitric oxide radical scavenging assay, which were studied by using ascorbic acid as standard. Results： The extract showed significant activities in all antioxidant assays compared with the reference antioxidant ascorbic acid. The total antioxidant activity as well as the reducing power was also found to increase in a dose-dependent manner. Conclusion： Evolvulus alsinoides may act as a chemopreventive agent, providing antioxidant properties and offering effective protection from free radicals.
文摘Objective:To select the appropriate concentrations of methanolic pomegranate extract supplemented in rams’semen extender for obtaining the best-cryopreserved semen quality.Methods:Tris-based semen extender was supplemented with 0.0,0.40,0.48,and 0.56 mg/mL pomegranate peel methanolic extract to extend semen collected from five native rams twice weekly for two months(n=80).Pooled(n=16)post-thaw semen characteristics were determined.Thawed seminal plasma of all supplemented and control groups were used to measure malondialdehyde(MDA),nitric oxide(NO),glutathione peroxidase(GPx),superoxide dismutase(SOD),ascorbic acid,zinc,copper,total cholesterol,low-density lipoproteins(LDL),lactate dehydrogenase(LDH),and alkaline phosphatase(ALP).Results:The supplementation of Tris-based semen extender with 0.48 mg/mL semen extender resulted in the highest post-thaw sperm total motility(P<0.001),sperm progressive motility(P<0.001),live sperm(P<0.001),sperm plasma membrane integrity(P<0.001),acrosome integrity(P<0.001),SOD(P<0.05),zinc(P<0.001),total cholesterol(P<0.001),and LDL(P<0.001)with the lowest percentage of abnormal sperm morphology(P<0.001),the lowest lipid peroxidation(MDA,P<0.01),ascorbic acid(P>0.05),and LDH(P>0.05).Conclusions:Pomegranate peel methanolic extract 0.48 mg/mL supplemented to Tris-based semen extender of rams is the best enrichment in preserving the sperm post-thaw characteristics via improving biochemical profiles and antioxidant capacity.
文摘Objective:To compare the antibacterial activity and antiseptic effect of ethanol extract of Peganum harmala L.(P.harmala)seeds and Betadine on eight reference strains of Streptococcus pneumoniae,Salmonella typhimurium,Escherichia coli,Corynebacterium pseudotuberculosis,Klebsiella pneumoniae,Staphylococcus aureus,Micrococcus luteus which are known to cause different types of skin infections,and Corynebacterium pseudotuberculosis isolated from an abscess on horse's neck.Methods:The antibacterial activity was assessed using a disc diffusion method.The minimum inhibitory concentration was tested by serial dilution method and the percentage of bacterial growth inhibition by absorbance microplate reader device.The minimum bactericidal concentration was then recorded.Clinical outcomes were obtained through washed up healing time of longitudinal and surface skin on the back of 16 rats with concentrations of 10,50,100,150 and 500 mg/mL of P.harmala extract.Results:The results of in vitro experiments showed that the lowest minimum inhibitory concentration(0.68-1.3 mg/mL)and minimum bactericidal concentration(1.3-5 mg/mL)values were observed in the ethanol extract of P.harmala seeds.Also the results of in vivo experiment showed that wound healing in the concentration of 50 mg/mL of this plant extract was better and quicker than Betadine.Conclusions:This study confirmed that ethanol extract of P.harmala has appropriate effect on the microorganisms and the healing of skin wounds in comparison with Betadine.
基金The animal protocols were approved by the Ethics Committee of the Second Affiliated Hospital of Harbin Medical University(SYDW2019-258).
文摘Background and objective:In northern China's cold regions,the prevalence of metabolic dysfunction-associated steatotic liver disease(MASLD)exceeds 50%,significantly higher than the national and global rates.MASLD is an important risk factor for cardiovascular and cerebrovascular diseases,including coronary heart disease,stroke,and tumors,with no specific therapeutic drugs currently available.The ethanol extract of cassia seed(CSEE)has shown promise in lowering blood lipids and improving hepatic steatosis,but its mechanism in treating MASLD remains underexplored.This study aims to investigate the therapeutic effects and mechanisms of CSEE.Methods:MASLD models were established in male Wistar rats and golden hamsters using a high fat diet(HFD).CSEE(10,50,250 mg/kg)was administered via gavage for six weeks.Serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),as well as liver TC and TG,were measured using biochemical kits.Histopathological changes in the liver were evaluated using Oil Red O staining,Hematoxylin-eosin(H&E)staining,and transmission electron microscopy(TEM).HepG2 cell viability was assessed using the cell counting kit-8(CCK8)and Calcein-AM/PI staining.Network pharmacology was used to analyze drug-disease targets,and western blotting was used to confirm these predictions.Results:CSEE treatment significantly reduced serum levels of TC,TG,LDL-C,ALT,and AST,and improved liver weight,liver index,and hepatic lipid deposition in rats and golden hamsters.In addition,CSEE alleviated free fatty acid(FFA)-induced lipid deposition in HepG2 cells.Molecular biology experiments demonstrated that CSEE increased the protein levels of p-AMPK,p-ACC,PPARα,CPT1A,PI3K P110 and p-AKT,while decreasing the protein levels of SREBP1,FASN,C/EBPα,and PPARγ,thus improving hepatic lipid metabolism and reducing lipid deposition.The beneficial effects of CSEE were reversed by small molecule inhibitors of the signaling pathways in vitro.Conclusion:CSEE improves liver lipid metabolism and reduces lipid droplet deposition in Wistar rats and golden hamsters with MASLD by activating hepatic AMPK,PPARα,and PI3K/AKT signaling pathways.
基金Supported by National Natural Science Foundation of China(30560119)Supported by Natural Science Foundation of Guangxi(0991030)+3 种基金Innovational Plan Project for Guangxi Postgraduate Ed-ucation(2008105930817M46)Project of Science Research Project of Guangxi Education Department([2006]26),Project of Science Research Project of Guangxi Education Department(200708LX180)Scientific Researc-hing fund project of Guangxi University(X081020)Fund Project for Guangxi Young People(0832008)~~
文摘[Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources.[Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC.The HPLC conditions were as follows;Alltima NH2 （5 μm,4.6 mm × 150 mm） column separation;the mobile phase was acetonitrile-2%H3PO4 （90：10）;the flowing velocity was 1 ml/min;test wavelength was 213 nm,the width of belt was 16 nm;reference wavelength was 300 nm,the width of belt was 80 nm.[Result] By the single factor and orthogonal tests,the optimum conditions were found as follows：ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1：25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%.[Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.
文摘Objective To investigate the antidiabetic, antioxidant and hypolipidemic efficacy of Cynodon dactylon in diabetic rats. Methods The experimental rats were randomly divided into three groups： Group I： control; Group II： Alloxan diabetic, untreated; and Group III： Alloxan diabetic treated with ethanolic extract of C. dactylon leaves （450 mg/kg bw）. Experimental diabetes was induced by alloxan in a single dose of 150 mg/kg bw. Results A Significant diminution of fasting blood sugar level was observed and also significant increase in HDL and decrease （P0.05） in cholesterol, triglyceride, LDL and VLDL were observed after 15 days of treatment. The investigation also revealed, the activities of AST, ALT, ALP, AP, LDH, and CPK （P0.05） were decreased in the extract‐supplemented group. The significant decrease in protein content and SOD, CAT, GPx, and GSH （P0.05） activity and increase in LPO in plasma were found to be ameliorated after treatment. Conclusion Our result supports the fact that administration of extract of C. dactylon leave is able to reduce hyperglycemia and hyperlipidemia risk and also reduced the oxidative stress in diabetic rats.
基金Supported by Key Projects in the National Science &Technology Pillar Program during the Eleventh Five-year Plan Period(2006BAD02B06)Projects in Sichuan Province Administration of Traditional Chinese Medicine (2006750)~~
文摘[Objective]The aim was to study the sedative and hypnotic effects of Tartary Buckwheat ethanol extract and lay a foundation for further expanding the application of Tartary Buckwheat. [Method]The effects of Tartary Buckwheat ethanol extract on pentobarbital sodium and mice spontaneous action times were recorded. [Result]Ethanol extract of Tartary Buckwheat could prolong mice sleep duration caused by pentobarbital sodium （suprathreshold dosage）,increase mice sleep number caused by pentobarbital sodium （subthreshold dosage）,and obviously inhibit mice spontaneous action times. [Conclusion]Ethanol extract of Tartary Buckwheat has a function of sedation.
基金Supported by National Key Technology R&D Program(2006BAD03A1505)National Forestry Bureau Construction Project([2007]Number527)~~
文摘[Objective] The aim was to compare the molluscicide activity of ethanol extract of Sapium sebiferum,Pterocarya stenoptera and Cryptomeria fortunei.[Method] The molluscicide activity of ethanol extract of S.sebiferum,P.stenoptera and C.fortunei was determined through poison test of Oncomelania hupensis in laboratory.[Result] S.sebiferum,P.stenoptera and C.fortunei had certain molluscicide effect,and S.sebiferum and C.fortunei had better effect with little difference than P.stenoptera;ethanol could extract the effective molluscicide component from plant more completely,and its molluscicide effect was better than that of plant infusion.[Conclusion] Our study could provide theory reference for the plant selection of controlling O.hupensis ecologically.
文摘Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride(CCl_4) induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperitoneal injection of CCl_4,1 mL/kg body weight for every 72 h.The ethanolic extract of Premna corymbosa leaves were administrated at doses of 200 & 400 mL/kg body weight, p.o.,daily for 14 days.The hepatotoxicity and its prevention was assessed by serum markers like serum alkaline phosphatase(SALP),serum triglycerides(STG),serum total protein(STP), serum cholesterol(SC),and liver wet weight and histopathological studies of the liver.Results:In treatment with the ethanolic extract,the toxic effect of CCl_4 was controlled significantly(P<0.01) by restoration of the levels of biochemical parameters as compared to normal and standard drug silymarin treated groups.The liver weight was reduced by the ethanolic extract treated groups. The histopathology of the liver sections evidenced the hepatoprotective activity.Conclusion:The ethanolic extract of the leaves of Premna corymbosa possess significant acute hepatoprotective activity.Premna corymbosa can be recommended for the liver disorders.
文摘Ethanolic extracts of five plant material from Eucalyptus gluaca, Melia azadrach, Mentha arvensis, Olea europaea leaves and periearp of Punica granatum were investigated for insecticidal activity against the confused flour beetle Tribolium confusum DuVal in the laboratory. Different concentrations 2.5%, 5%, 7.5%, 10% using filter paper test were implemented in the experiment. Mortality was assessed after 24, 48, 72 hrs of treatment and LDs0 were calculated at respective time intervals. Data analysis was made by using factorial Complete Randomized Design （CRD）. The results revealed that all of the tested materials with some variations had lethal effects against the pest as compared with the untreated check. The time 24 h showed higher insecticidal effect with the tested concentrations for the larvae and adult of Tribolium confusum. Strong toxicity effect was observed at concentration 5% of M.. arvensis for adult followed by E. gluaca, O. europaea, M.. azedrach and P. granatum. For larval stage the extract of O. europaea and E. gluaca with concentration 7.5%, 1%, respectively caused high mortality followed by M. arvensis, M. azedrach and P. granatum. The LDs0 values for adults and larvae were calculated to be 1.33 and 1.42 with M.. arvensis and O. europaea, respectively.
文摘The purpose of the study was to evaluate the efficiency of the microwave (MW) extraction method by comparing it with a conventional method through evaluation of antimicrobial and synergism/antagonism activity of each aquatic and ethanolic extracts samples, which were extracted from the dried plants (<em>Ficus sycomorus</em> leaves, <em>Lawsonia inermis</em> leaves and <em>Glycerrhiza glabra</em> Linn.). Nine samples of each plant for both solvents were irradiated with MW at several power outputs (180 w, 360 w, and 540 w) in several interval times (1, 2 and 3 minutes). The antibacterial activities of extracts and the synergistic effect between plants and antibiotics were evaluated using disk diffusion method against clinical isolated <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. The result revealed that the inhibition zone for more than 50% using aquatic and ethanolic samples results (extracted in two minutes and MW power 180 w) had shown the optimum extract and better antibiotic activity for each plant. Also, the results of ethanolic extracts used against selected microorganisms showed antimicrobial and synergistic effect with most antibiotics better than aquatic extracts. Our results indicate the possibility of using MW apparatus as an extractor to obtain bioactive compounds from plants and thus used in the treatment of bacterial infections, and some results of this study were encouraging. However, the antagonistic reactions of some extracts with some antibiotics and their use in combination should be further studied for<em> in vitro</em> activities. It is clearly a need to be furthermore evaluated, to identify the effective components, the mode of action and the possible toxic effect <em>in-vivo</em> of these ingredients.
文摘Lagenaria siceraria （Molina） Standl., known as bottle gourd is widely available throughout Vietnam as an edible vegetable. Bottle gourd is a commonly fruit but there are little reports about its biological activities. The aim of the present study was to evaluate antioxidant activity and anti-hyperglycemic potential of ethanolic extract of bottle gourd. The ethanolic extract was tested for its antioxidant activity on chelating power, the ferric reducing/antioxidant power assay （FRAP） and the 1, 1-diphenyl-2-picryl-hydrazyl （DPPH） free radical scavenging assay. The results showed that chelating activity was 80.02%, FRAP value was 92.84 μmol Fe^2＋/g and active radical scavenging which showed an IC50 was 49.50 μg/ml. The five different doses of ethanolic extract were treated by oral tolerance to mice body before oral glucose tolerance and blood glucose level was measured using glucose oxidase method. The result indicated that inhibition percentage of ethanolic extract of bottle gourd at the dose of 400 mg/kg in glucose-induced hyperglycemic mice was effectively similar to standard drug glibenclamide. The results obtained indicate that ethanolic extract from bottle gourd is the potential antioxidant and anti-hyperglycemic agent.
文摘Plants of Baccharis （Asteraceae） genus are commonly known in Argentina as ＂carqueja＂. The antimicrobial activity and minimal inhibitory concentration of B. articulata, B. trimera and B. crispa aqueous and ethanolic extracts were evaluated by using the micro-well dilution method. Previously, the components of extracts were analyzed by spectroscopial means. Gram-positive bacteria were more sensitive to Baccharis species extracts than Gram-negative bacteria. Out of 3 plant species, B. trimera showed significant antibacterial activity and aqueous and ethanolic extracts were active against Staphylococcus aureus （MIC = 2,500 μg/mL and 1,250 μg/mL, respectively） and Listeria monocytogenes （MIC = 625 μg/mL and 625 μg/mL, respectively）. All ethanolic extracts inhibited the growth of the selected Gram-positive （MIC values ranged between 625 μg/mL and 1,250 μg/mL）. Therefore, all Gram-negative bacteria were resistant to the ethanolic and aqueous extracts tested. One flavone, genkawanin, was identified from the three ethanolic extracts as the responsible of antibacterial activity. Two terpenes, hawtriwaic acid and bacrispine, were identified from ethanolic extract of B. crispa and B. trimera as the responsibles of antibacterial activity. These preliminary studies corroborated the antimicrobial activity of the selected plants used in folklore medicine. Therefore, they could be potential sources of new antimicrobial agents used in treatment of infectious diseases.
