目的:探讨人乳头状瘤病毒(HPV) E6/E7 mRNA与液基薄层细胞学检查(TCT)联合检测在宫颈癌风险预测中的应用价值。方法:选取2023年1月至2024年6月于延安市人民医院行宫颈癌和癌前病变筛查的216例患者作为研究对象,对其资料进行回顾性分析,...目的:探讨人乳头状瘤病毒(HPV) E6/E7 mRNA与液基薄层细胞学检查(TCT)联合检测在宫颈癌风险预测中的应用价值。方法:选取2023年1月至2024年6月于延安市人民医院行宫颈癌和癌前病变筛查的216例患者作为研究对象,对其资料进行回顾性分析,该部分患者均同时行HPV E6/E7 mRNA与液基薄层细胞学检测联合及组织病理学检查,分析HPV E6/E7 mRNA与液基薄层细胞学单独检测及两者联合检测宫颈癌的灵敏性、特异性、阳性预测值及阴性预测值。结果:216例患者中,HPV E6/E7mRNA与液基薄层细胞学单独检测的敏感性、特异性、阳性预测值、阴性预测值分别为78.41%、42.50%、85.71%、30.91%和71.59%、62.50%、89.36%、33.33%;HPV E6/E7 mRNA与液基薄层细胞学联合检测的敏感性、特异性、阳性预测值、阴性预测值为93.19%、65.00%、92.13%、68.42%,差异均有统计学意义(P Purpose: To explore the application value of HPV E6/E7 mRNA and Thinprep cytologic test (TCT) in predicting the risk of cervical cancer. Methods: 216 patients who underwent HPV E6/E7 mRNA and TCT at Yan’an People’s Hospital from January 2023 to June 2024 were selected as the study subjects. The data were retrospectively analyzed. All patients underwent HPV E6/E7 mRNA combined with TCT and histopathological examination. The sensitivity, specificity, positive predictive value, and negative predictive value of HPV E6/E7 mRNA and TCT detected separately and combinedly for cervical cancer were analyzed. Results: Among 216 patients, the sensitivity, specificity, positive predictive value, and negative predictive value of HPV E6/E7 mRNA and TCT detected separately were 78.41%, 42.50%, 85.71%, 30.91% and 71.59%, 62.50%, 89.36% and 33.33%;The sensitivity, specificity, positive predictive value, and negative predictive value of combined detection of HPV E6/E7 mRNA and TCT were 93.19%,65.00%, 92.13%, and 68.42%, with statistically significant differences (P < 0.05). Conclusion: The combined detection of HPV E6/E7 mRNA and TCT can effectively improve the positive detection rate of cervical cancer and precancerous lesions, significantly increase sensitivity and negative predictive value, and have good consistency with the gold standard for biopsy. It can effectively determine the infection status and severity of HPV, reduce unnecessary colposcopy biopsy, reduce the psychological burden of patients, and have good application value in predicting the risk of cervical cancer.展开更多
BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human p...BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.展开更多
文摘目的:探讨人乳头状瘤病毒(HPV) E6/E7 mRNA与液基薄层细胞学检查(TCT)联合检测在宫颈癌风险预测中的应用价值。方法:选取2023年1月至2024年6月于延安市人民医院行宫颈癌和癌前病变筛查的216例患者作为研究对象,对其资料进行回顾性分析,该部分患者均同时行HPV E6/E7 mRNA与液基薄层细胞学检测联合及组织病理学检查,分析HPV E6/E7 mRNA与液基薄层细胞学单独检测及两者联合检测宫颈癌的灵敏性、特异性、阳性预测值及阴性预测值。结果:216例患者中,HPV E6/E7mRNA与液基薄层细胞学单独检测的敏感性、特异性、阳性预测值、阴性预测值分别为78.41%、42.50%、85.71%、30.91%和71.59%、62.50%、89.36%、33.33%;HPV E6/E7 mRNA与液基薄层细胞学联合检测的敏感性、特异性、阳性预测值、阴性预测值为93.19%、65.00%、92.13%、68.42%,差异均有统计学意义(P Purpose: To explore the application value of HPV E6/E7 mRNA and Thinprep cytologic test (TCT) in predicting the risk of cervical cancer. Methods: 216 patients who underwent HPV E6/E7 mRNA and TCT at Yan’an People’s Hospital from January 2023 to June 2024 were selected as the study subjects. The data were retrospectively analyzed. All patients underwent HPV E6/E7 mRNA combined with TCT and histopathological examination. The sensitivity, specificity, positive predictive value, and negative predictive value of HPV E6/E7 mRNA and TCT detected separately and combinedly for cervical cancer were analyzed. Results: Among 216 patients, the sensitivity, specificity, positive predictive value, and negative predictive value of HPV E6/E7 mRNA and TCT detected separately were 78.41%, 42.50%, 85.71%, 30.91% and 71.59%, 62.50%, 89.36% and 33.33%;The sensitivity, specificity, positive predictive value, and negative predictive value of combined detection of HPV E6/E7 mRNA and TCT were 93.19%,65.00%, 92.13%, and 68.42%, with statistically significant differences (P < 0.05). Conclusion: The combined detection of HPV E6/E7 mRNA and TCT can effectively improve the positive detection rate of cervical cancer and precancerous lesions, significantly increase sensitivity and negative predictive value, and have good consistency with the gold standard for biopsy. It can effectively determine the infection status and severity of HPV, reduce unnecessary colposcopy biopsy, reduce the psychological burden of patients, and have good application value in predicting the risk of cervical cancer.
基金Scientific Research Project of Hubei Provincial Health Commission,No.WJ2021M189。
文摘BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical cancer.Early detection and treatment can reduce the incidence of cervical cancer.AIM To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and diagnosis.METHODS One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected.These patients were graded CIN based on colposcopy and cervical pathology.The positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all groups.Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr.The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.RESULTS The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,respectively.Sensitivity and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was 0.789.Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.
